Application of Cordyceps sobolifera water extract in preparation of acute liver injury preventing and treating drug

A technology of acute liver injury and water extract, which is applied in the field of preparation of medicines for preventing and treating acute liver injury by water extract of cicada flower, can solve the problem that the effect of medicine and the relevant mechanism of action are not yet clear, and can protect liver function and relieve liver inflammation. Response, the effect of prevention and treatment of acute liver injury

Inactive Publication Date: 2017-05-24
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been reported in the literature that cicadae has anti-tumor, anti-oxidation, immune-enhancing, liver-protecting and anti-inflammatory effects, but its specific pharmacological effects and related mechanisms have not yet been clarified.

Method used

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  • Application of Cordyceps sobolifera water extract in preparation of acute liver injury preventing and treating drug
  • Application of Cordyceps sobolifera water extract in preparation of acute liver injury preventing and treating drug
  • Application of Cordyceps sobolifera water extract in preparation of acute liver injury preventing and treating drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Experiment of the protective effect of cicadae flower water extract on acute liver injury model

[0027] Grouping: SPF grade C57BL / 6 male mice were randomly divided into 6 groups, namely: ① normal control group; ② model group; ③ positive drug group; ④ low-dose cicada flower water extract group; Dosage group; ⑥ high-dose group of cicadae flower water extract; administration: ③ two weeks with bicyclol 200mg / kg orally administered, ④-⑥ with 100mg / kg, 200mg / kg, 400mg / kg of cicadae flower water The extract was given by intragastric administration for two weeks, and ①-② was given the same amount of drinking water as a control. Modeling and material collection: D-GalN / LPS solution (600mg / kg, 30μg / kg) was injected intraperitoneally to create a model of acute liver injury. Eight hours later, the eyeball was removed to collect blood, and the liver was quickly separated by laparotomy. Detection of ALT, AST and ALP activities in serum; preparation of liver homogenate, ac...

Embodiment 2

[0029] Embodiment 2 The influence of cicadae flower water extract on liver pathology

[0030] Grouping: SPF grade C57BL / 6 male mice were randomly divided into 6 groups, namely: ① normal control group; ② model group; ③ positive drug group; ④ low-dose cicada flower water extract group; Dosage group; ⑥ high-dose group of cicadae flower water extract; administration: ③ two weeks with bicyclol 200mg / kg orally administered, ④-⑥ with 100mg / kg, 200mg / kg, 400mg / kg of cicadae flower water The extract was given by intragastric administration for two weeks, and ①-② was given the same amount of drinking water as a control. Modeling and material collection: D-GalN / LPS solution (600mg / kg, 30μg / kg) was injected intraperitoneally to create an acute liver injury model. After 8 hours, the liver was separated by laparotomy, and the right lobe of the liver was cut off about 3.5mm×3.5mm. After paraformaldehyde fixation, gradient dehydration, routine paraffin embedding, and HE staining after sectio...

Embodiment 3

[0032] Example 3 The effect of cicadae flower water extract on liver neutrophil infiltration

[0033] Grouping: SPF grade C57BL / 6 male mice were randomly divided into 6 groups, namely: ① normal control group; ② model group; ③ positive drug group; ④ low-dose cicada flower water extract group; Dosage group; ⑥ high-dose group of cicadae flower water extract; administration: ③ two weeks with bicyclol 200mg / kg orally administered, ④-⑥ with 100mg / kg, 200mg / kg, 400mg / kg of cicadae flower water The extract was given by intragastric administration for two weeks, and ①-② was given the same amount of drinking water as a control; modeling and material collection: acute liver injury model was induced by intraperitoneal injection of D-GalN / LPS solution (600mg / kg, 30μg / kg) for 8 hours Afterwards, the liver was separated by laparotomy, liver homogenate was prepared, and MPO activity in the liver homogenate was detected;

[0034] The results showed that the water extract of cicadae can signif...

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Abstract

The invention belongs to the field of traditional Chinese medicine production and relates to an application of Cordyceps sobolifera water extract in preparation of an acute liver injury preventing and treating drug. Based on D-galactosamine/lipopolysaccharide induced acute liver injury model experiments, results prove that the Cordyceps sobolifera water extract can relieve lipid peroxidation and oxidative stress injury of liver cells by reducing transaminase (AST and ALT) and alkaline phosphatase (ALP) in serum, remarkably relieves infiltration of inflammatory factors of the liver and improves liver lesion obviously, meanwhile, the Cordyceps sobolifera water extract can relieve infiltration of neutrophils in the liver, inhibit transcription and expression of related inflammatory mediators, reduce inflammation induced inflammatory factor and NO release and inhibit MAPK (mitogen-activated protein kinases) pathway activation. The Cordyceps sobolifera water extract can be used for preparing the acute liver injury preventing and treating drug and is used for protecting liver function and relieving liver inflammation.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine pharmacy, and relates to the new medicinal use of the water extract of the Chinese herbal medicine cicadae, in particular to the use of the water extract of the cicadae in preparing medicines for preventing and treating acute liver injury. Background technique [0002] According to reports, acute liver injury has a sudden onset and rapid development, which can lead to multiple organ failure and even life-threatening. Therefore, it is a serious topic that has attracted great attention of those skilled in the art in clinical research practice. Studies have confirmed that the occurrence and development of acute liver injury is accompanied by an obvious inflammatory response, including the generation of inflammatory factors, the accumulation and infiltration of macrophages and immune cells in the liver, and the activation of related inflammatory signaling pathways. Usually, clinical practic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/066A61P1/16A61K35/64
Inventor 朱依谆杨少薇
Owner FUDAN UNIV
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