The invention relates to a human immunity improving traditional Chinese medicine preparation and a preparation method. The method includes: a, preparing culture medium mixed solution; b, sterilizing;c, cooling, d, performing inoculation to obtain fermented liquid; c, performing standing treatment to obtain the human immunity improving traditional Chinese medicine preparation. The human immunity improving traditional Chinese medicine preparation is capable of improving an in-vivo micro-ecological environment, balancing and regulating intestinal probiotics, repairing damaged cells, replenishingvarious in-vivo active bio-enzymes, amino acids, nucleic acids, trace elements and the like, improving human immunity and preventing diseases. In addition, the human immunity improving traditional Chinese medicine preparation is capable of improving acidic physique into alkalescent physique, can be used for preparing pills, ointments, aqueous solutions and the like and has a great auxiliary treatment effect in human immunity improvement.