105 results about "Cordyceps sobolifera" patented technology

The invention discloses a method for artificially culturing paecilomyces cicadae and application of a culturing product thereof. The method for artificially culturing paecilomyces cicadae in large scales comprises the steps of: preparing strains, dosing and packing into a box, sterilizing, inoculating, solid-fermenting, collecting and the like. The paecilomyces cicadae is cultured by utilizing grains, such as corn flour, bran, wheat, barley, rice, millet, broomcorn and the like and culture mediums of bagasse, cane sugar, shell powder, silkworm chrysalis meal and potassium nitrate. The raw materials are obtained from local resources, a large amount of production cost is saved, the culturing period is shortened, and cordyceps sobolifera obtained by culturing has the advantages of high quality and favorable stability and the like. A culture obtained by the invention can be used for preparing foods, health-care products, drugs and cosmetics with functions of fighting tumor, regulating immunity, reducing blood sugar, blood fat and blood pressure, improving eye sight, resisting radiation, dispelling heat and easing pains, calming and hypnotizing, nourishing and strengthening, improving kidney function and the like.

The invention discloses a bionic culture method for cordyceps sobolifera, which includes fungus separation, screening, spore culture, infected body impregnation, medium culture, harvest and other steps and culture conditions thereof. The method can simulate the wild ecological environment and overcome the limit of the growth period and season of wild cordyceps sobolifera, mass culture is easy, the adenosine content of the cultured bionic cordyceps sobolifera is higher than the adenosine content of wild cordyceps sobolifera, the quality is high, and the bionic cordyceps sobolifera has extremely high market popularization value.

The invention discloses a preparation method of a cordyceps sobolifera and burdock oral liquid, and belongs to the technical field of extraction of nutritional components of plants. The preparation method of the cordyceps sobolifera and burdock oral liquid comprises the following steps: (1) preparing cordyceps sobolifera cordyceps liquid seeds; (2) cultivatiing cordyceps sobolifera cordyceps; (3) extracting after the materials are smashed with hot water and carrying out homogenate; (4) carrying out high shear and superfine grinding with wet method; (5) hydrolyzing cellulose, amylase, and pectinase; (6) filtering, concentrating and filling to obtain the cordyceps sobolifera and burdock oral liquid. The preparation method is short in product production cycle, avoids raw material loss in product production process and waste discharge in production process, and is an environment-friendly production method with a high product output rate and simple production technology; besides, as health care type functional food, the cordyceps sobolifera and burdock oral liquid can be drunk for a long time as daily health care, can be used as a product for the adjuvant therapies of related diseases, and broadens the application fields of cordyceps sobolifera cordyceps and burdock root.

The invention discloses a coffee flavor health-maintaining sea cucumber and dendrobium officinale solid beverage. The coffee flavor health-maintaining sea cucumber and dendrobium officinale solid beverage is prepared from the following raw materials in parts by weight: 4-6 parts of sea cucumbers, 8-10 parts of ground coffee, 22-25 parts of dendrobium officinale, 11-13 parts of broccolis, 3-5 parts of yellow rice or millet wine, 9-11 parts of rock candy, 1-2 parts of erycibe obtusifolia benth, 7-9 parts of amomum kravanh, 10-12 parts of tomato juice, 8-10 parts of raspberries, 3-4 parts of cordyceps sobolifera, 14-16 parts of buckwheat powder, 1-2 parts of fennels, 6-7 parts of pollen pini, 6-7 parts of walnut protein powder, 7-9 parts of corn soybean milk, 3-4 parts of enteromorpha, 1-2 parts of lysimachia christinae hance, 9-12 parts of a nutritional supplement and a suitable amount of water. The coffee flavor health-maintaining sea cucumber and dendrobium officinale solid beverage has rich coffee flavor, and savory and mellow taste; dendrobium officinale has the effects of promoting the secretion of saliva or body fluid and nourishing stomach, moistening lung and tonifying kidney, and improving eyesight; furthermore, the content of proteins, calcium, potassium, zinc, iron, selenium and manganese is high and a plurality of amino acids are contained, so that the beverage has the effects of enriching blood and moistening dryness; and the coffee flavor health-maintaining sea cucumber and dendrobium officinale solid beverage is suitable for people with low immunity, anepithymia, palpitation and shortness of breath and dizziness to drink and has high nutritive value and good health effect.

The invention discloses cordyceps cicadae/cordyceps sobolifera mycelium active substance and a composition for protecting nerve cells. Under the noncytotoxic concentration of 40 mu/ml, the cordyceps cicadae/cordyceps sobolifera mycelium active substance can be used for protecting the neurotoxicity of MPP+ to the dopaminergic neuron, and also can be used for inhibiting the cell apoptosis caused by the pressure of the endoplasmic reticulum caused by tunicamycin, and therefore, the cordyceps cicadae/cordyceps sobolifera mycelium active substance has the potential of preventing neurodegenerative diseases.

The invention discloses a culture method of selenium-enriched cordyceps sobolifera conidial powder. Firstly, separation, purification and identification are performed on cordyceps sobolifera collected in a field to obtain Paecilomyces cicadae (Miquel.) Samson, plate cultivation is performed to obtain single colonies, then, the single colonies are transferred to a liquid medium for culture, a liquid secondary seed solution is obtained, the secondary seed solution is transferred to a composite culture medium containing organic selenium fertilizer, the temperature is controlled to 15-35 DEG C, the humidity is controlled to 60-90%, ventilation culture is performed for 4-6 weeks, and harvesting is performed to obtain selenium-enriched cordyceps sobolifera conidial powder. Artificial simulation is performed to obtain nutritional and growth conditions closest to the conditions needed by cordyceps sobolifera wild environment, growth of cordyceps sobolifera is not limited by season changes, the composite culture medium is closer to natural bamboo forest cordyceps sobolifera wild environment, the breathability is good, the water absorption and moisturizing performance are excellent, a fruiting body grows rapidly, spore head and conidial powder of the fruiting body are abundant, thick, solid, full, large in conidial powder amount, high in quality and easy to harvest, the content of selenium is 20-100 mg/Kg, the method is simple and low in cost, the spore harvesting period is short, and industrial production can be achieved.

The invention relates to an artificial cultivation method of cordyceps sobolifera. The method comprises the following steps: step 1, expanded cultivation of cordyceps sobolifera strains; step 2, soil culture of strains obtained by expanded cultivation in the step 2, step 3, harvesting of coremium. In the step, the soil culture comprises dark culture and light culture phases. In the light culture, green light is utilized for irradiation. The method has the following beneficial effects: in the phase of cordyceps sobolifera cultivation, green light (single-color light) is utilized for irradiation; compared with white composite light, the yield of coremium is greatly increased.

The invention discloses a method for preparing health cordyceps sobolifera yellow wine with a cordyceps sobolifera culture medium, and belongs to the technical field of biology. The health cordyceps sobolifera yellow wine is prepared by adding the cordyceps sobolifera culture medium which is subjected to high-speed crushing and ultrasonic cell breakage and cylinder flushing water at the same time during cylinder flushing for fermentation with sticky rice serving as a raw material, to fully extract effective components of cordyceps sobolifera in the cordyceps sobolifera culture medium. Therefore, the prepared health cordyceps sobolifera yellow wine has certain effects of enhancing the immunity of a human body, resisting aging and preventing diseases; meanwhile, due to the full use of the cordyceps sobolifera culture medium prepared from harvested and cultured cordyceps sobolifera, the economic benefit of cordyceps sobolifera cultivation is increased.

The invention belongs to the technical field of artificial cultivation of edible mushrooms, and particularly relates to an artificial cultivation method for increasing the content of polysaccharide in cordyceps sobolifera sporocarp. According to the cultivation method for increasing the content of the cordyceps sobolifera polysaccharide in the cordyceps sobolifera sporocarp, on the basis of a conventional cordyceps sobolifera sporocarp cultivation process, dead leaves and pruned spindling leaves generated in succulent plants bred by themselves are added into a solid culture medium of cordyceps sobolifera, it is surprisingly found that in the solid fermentation process of cordyceps sobolifera, the dead leaves and leaves of the added succulent plants can effectively induce accumulation of the cordyceps sobolifera polysaccharide, and the content of the cordyceps sobolifera polysaccharide in the cordyceps sobolifera sporocarp is increased.

The invention provides a fermentation method of antioxidant cordyceps sobolifera mycelia. The fermentation method comprises the following steps: respectively preparing a PDA slant culture medium, a seed culture solution and a fermentation culture medium; dividing the paecilomyces cicadae mother strain through sterile operation, and inoculating the paecilomyces cicadae mother strain to the PDA slant culture medium for activation culture to obtain an activated strain; inoculating the activated strain into a seed culture solution to obtain a primary strain; inoculating the primary strain into theseed culture solution, and culturing the primary strain according to a method for culturing the activated strain to obtain a secondary strain; inoculating the primary strain or the secondary strain into the seed culture solution for culturing to obtain a fermentation tank seed solution; inoculating the fermentation tank seed solution into the fermentation culture medium, and carrying out fermentation culture to obtain cordyceps sobolifera fermentation liquor; and discharging the cordyceps sobolifera fermentation liquor, and carrying out semi-solid fermentation culture to obtain the fermentation product of the antioxidant cordyceps sobolifera mycelia. The fermentation method is suitable for fermentation production of cordyceps sobolifera mycelia, the obtained mycelia is high in biomass andstrong in antioxidant activity, the yield of active substance polysaccharide and cordycepic acid can be remarkably increased, and the source of health-care food raw materials is increased.

The invention belongs to the field of traditional Chinese medicine production and relates to an application of Cordyceps sobolifera water extract in preparation of an acute liver injury preventing and treating drug. Based on D-galactosamine/lipopolysaccharide induced acute liver injury model experiments, results prove that the Cordyceps sobolifera water extract can relieve lipid peroxidation and oxidative stress injury of liver cells by reducing transaminase (AST and ALT) and alkaline phosphatase (ALP) in serum, remarkably relieves infiltration of inflammatory factors of the liver and improves liver lesion obviously, meanwhile, the Cordyceps sobolifera water extract can relieve infiltration of neutrophils in the liver, inhibit transcription and expression of related inflammatory mediators, reduce inflammation induced inflammatory factor and NO release and inhibit MAPK (mitogen-activated protein kinases) pathway activation. The Cordyceps sobolifera water extract can be used for preparing the acute liver injury preventing and treating drug and is used for protecting liver function and relieving liver inflammation.

The invention discloses paecilomyces cicadae and application in preparing an antibacterial agent, a probiotic preparation and an antibacterial fresh-keeping film. A novel strain-paecilomyces cicadae CH 113.1 is first separated from field entomogenous fungus cordyceps sobolifera. A strain fermentation product has an inhibition effect for conventional pathogenic microorganisms, the inhibition rate for the escherichia coli can reach 70 percent or more, and the biological bacteriostatic agent can be applied to the fresh keeping of food. The strain has a good application prospect in the field of food.

The invention discloses a method for using a bamboo forest to cultivate cordyceps sobolifera. The method includes: building a site suitable for the gathering and living of cicadas, sowing cordyceps sobolifera strains in the bamboo forest after the cordyceps sobolifera strains are cultivated artificially, using light to attract the cicadas together to allow cicada larva to be infected by the cordyceps sobolifera strains when the cicada larva falls into the ground so as to obtain cordyceps sobolifera, in other words, selecting the bamboo forest suitable for the cicadas to live so as to create good environmental conditions for copulation, egg laying and larva growth of the cicadas, and allowing the cordyceps sobolifera strains to reproduce massively in the bamboo forest through artificial cultivation and cordyceps sobolifera strain sowing so as to allow the cicada larva to be infected by the cordyceps sobolifera strains when the cicada larva falls into the ground and increase the yield of the cordyceps sobolifera. The method has the advantages that the natural bamboo forest is fully utilized to create the environment which is benefit for the living of the cicadas, the number of the cordyceps sobolifera strains is increased through artificial sowing, yield of the natural cordyceps sobolifera is increased, the cordyceps sobolifera can be harvested for several years after the one-time sowing, and promising application prospect is achieved.

The invention relates to the field of biotechnology and discloses a production method for Cordyceps sobolifera mycelium and exopolysaccharide liquid fermentation. The production method comprises: using a strain of Cordyceps sobolifera as a starting strain, and acquiring mycelium and exopolysaccharide by liquid deep fermentation; grinding a test tube slant strain under aseptic condition, inoculating to a liquid medium to prepare a liquid strain, subjecting the liquid strain to liquid deep fermentation, shake culturing or stir culturing to obtain a culture, and separating mycelium and a culture solution by means of centrifugation or filtration and the like to obtain mycelium (dry weight) 5-8 g/L; subjecting the culture solution to ultrafiltration or ethanol precipitation to obtain exopolysaccharide, 0.5-1.2 g/L in yield. The production of Cordyceps sobolifera mycelium and exopolysaccharide may be carried out using the above method, and this method has high development value.

The invention relates to cordyceps sobolifera micropowder tablets and a preparation method thereof. The cordyceps sobolifera micropowder tablets are tablets prepared from cordyceps sobolifera micropowder with the water content of 5% to 15%, the mesh of 1,000 to 10,000 and the particle size of 1.5 micrometers to 15 micrometers. The preparation method of the cordyceps sobolifera micropowder tablets comprises the following steps that 1, cordyceps sobolifera is crushed until the particle size is 1.5 micrometers to 15 micrometers and the water content is controlled to be 5% to 15%; 2, cordyceps sobolifera obtained in the step 1 is subjected to dry granulating, sieved with a 30-60-mesh sieve and then tableted, wherein a tabletting method comprises the step that the cordyceps sobolifera powder is prepressed before being tableted, the prepressing pressure is 0.8 kN to 30 kN, and the main pressure is 30 kN to 80 kN. According to the cordyceps sobolifera micropowder tablets and the preparation method thereof, the particle size of the powder obtained before cordyceps sobolifera tabletting is 1.5 micrometers to 15 micrometers, the dissolution rate is very high, the dissolution time is short, and the bioavailability is high.

The invention relates to the field of traditional Chinese medicine application and discloses a traditional Chinese medicine for treating liver and gallbladder damp-heat-type gallstone. The traditional Chinese medicine for treating liver and gallbladder damp-heat-type gallstone is prepared from Brandisia herb, medulla stachyuri, virgate wormwood, herb of common achyranthes, avicennia pricklyash root, broom cypress fruit, stringy stonecrop herb, Juncus roemerianus, semen nigellae, polyporus, hovenia dulcis thumb, coix seeds, rhizoma dioscoreae septemlobae, caulis clematidis armandii, fructus polygoni orientalis, curcuma tuber, snake bramble seed, rabdosia rubescens, imperata rhizome, pagodatree pod, biota orientalis, herba diplazii lancei, madder, herba cephalanoploris, bryophyllum, garden burnet, spiny amarauth herb or root, Chinese iris seed, pearl shell, cordyceps sobolifera, dogbane leaf and green tangerine peel as raw materials by mixing according to a certain ratio. The traditional Chinese medicine has the advantages of simple preparation processes, good absorption effects, long drug action time, substantial curative effects, no side effect, high safety, low production cost and timely remission of patient diseases.

The invention discloses a solid medium for producing plasmin by using cordyceps sobolifera and a plasmin production method using the same, belonging to the field of microbial technology. The solid medium comprises the following active ingredients in parts by weight: 8-15 parts of peptone, 30 parts of glucose, 1 part of monopotassium phosphate, 3 parts of yeast extract, 0.2 part of magnesium sulfate heptahydrate, 15g of agar and 1000 parts of water. A plasmin production method using the solid medium for producing plasmin by using cordyceps sobolifera comprises the following steps: (1) inoculating a paecilomyces cicadidae strain (B5) to a PDA panel and culturing; (2) sterilizing the prepared solid medium and pouring into a sterilized petri dish; cooling to obtain a solid panel; teasing the hypha block with a teasing needle, inoculating to the prepared solid inducing medium panel and culturing; and (3) putting the cultured bacterial colony in a refrigerator overnight; fetching the hypha block with medium and adding into sterile water; mashing and standing; performing high-speed centrifugal separation; and fetching the supernate as a crude extract of plasmin for later use.

The invention discloses an anti-helicobacter pylori probiotic composition as well as a preparation method and application thereof. The probiotic composition is prepared from the following raw materials in parts by weight of 60 to 80 parts of oat beta-glucan, 10 to 20 parts of probiotics, 1 to 4 parts of cordyceps sobolifera sporocarp, 1 to 4 parts of rice bran fatty alkanol, 1 to 4 parts of eucommia ulmoides male flower and 1 to 4 parts of psyllium husk. According to the composition, the oat beta-glucan is adopted to achieve the effect of protecting the probiotics, the dosage of the probiotics can be reduced, and the oat beta-glucan can promote the growth of the probiotics, so that the probiotics play a synergistic role; and meanwhile, the contained cordyceps sobolifera sporocarp (artificially cultivated), rice bran fatty alkanol, eucommia ulmoides male flower and psyllium husk all have the effect of inhibiting helicobacter pylori, so that helicobacter pylori infection is effectively prevented and improved.