Artificial cultivation method for increasing content of cordyceps sobolifera polysaccharide in cordyceps sobolifera sporocarp
A technology of Cordyceps fruiting bodies and Cordyceps cicadae, which is applied in cultivation, plant cultivation, mushroom cultivation, etc., can solve problems such as unsatisfactory polysaccharide content of Cordyceps cicadae, and achieve the effect of increasing the content and improving the nutritional value
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Embodiment 1
[0029] In this example, the seed liquid obtained above was cultured in a solid medium for fruiting bodies.
[0030] The solid matrix is prepared according to the following contents: 10 parts by weight of rice, 30 parts by weight of corn dregs, 10 parts by weight of bran, 20 parts by weight of cicada pupa powder, 5 parts by weight of dead leaves of Jiyueyue plant, crumbs of leaves of Jiyueyue plant 12 parts by weight, 0.5 parts by weight of EM bacteria powder (do not add temporarily);
[0031] Prepare the nutrient solution according to the following contents: glucose 20g / L, peptone 30g / L, potassium dihydrogen phosphate 2g / L, dipotassium hydrogen phosphate 1g / L, magnesium sulfate 2g / L;
[0032] According to the mass ratio of 1:0.8, mix the above solid medium (excluding EM bacteria powder) and nutrient solution, seal the prepared medium, place it in a high-pressure humid heat sterilizer, and sterilize it at 121°C for 40 minutes.
[0033] At room temperature and under sterile c...
Embodiment 2
[0037] In this example, the seed liquid obtained above was cultured in a solid medium for fruiting bodies.
[0038] The solid substrate is prepared according to the following contents: 20 parts by weight of rice, 20 parts by weight of corn dregs, 20 parts by weight of bran, 10 parts by weight of cicada pupa powder, 12 parts by weight of dead leaves of Jiyueyue plant, crumbs of leaves of Jiyueyue plant 5 parts by weight, 2 parts by weight of EM bacteria powder (do not add temporarily);
[0039] Prepare the nutrient solution according to the following contents: glucose 30g / L, peptone 20g / L, potassium dihydrogen phosphate 4g / L, dipotassium hydrogen phosphate 4g / L, magnesium sulfate 4g / L;
[0040] According to the mass ratio of 1:1.5, mix the above solid medium (excluding EM bacteria powder) and nutrient solution, seal the prepared medium, place it in a high-pressure humid heat sterilizer, and sterilize it at 121°C for 40 minutes.
[0041] At room temperature and under sterile co...
Embodiment 3
[0045] In this example, the seed liquid obtained above was cultured in a solid medium for fruiting bodies.
[0046] The solid substrate is prepared according to the following contents: 15 parts by weight of rice, 25 parts by weight of corn dregs, 15 parts by weight of bran, 15 parts by weight of cicada pupa powder, 8 parts by weight of dead leaves of Jiyueyue plant, crumbs of leaves of Jiyueyue plant 10 parts by weight, 1 part by weight of EM bacteria powder (do not add temporarily);
[0047] Prepare the nutrient solution according to the following contents: glucose 25g / L, peptone 25g / L, potassium dihydrogen phosphate 3g / L, dipotassium hydrogen phosphate 2g / L, magnesium sulfate 3g / L;
[0048] According to the mass ratio of 1:1.2, mix the above solid medium (excluding EM bacteria powder) and nutrient solution, seal the prepared medium, place it in a high-pressure humid heat sterilizer, and sterilize it at 121°C for 40 minutes.
[0049] At room temperature and under sterile con...
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