Paecilomyces cicadae and application

A technology of Paecilomyces cicadae and antibacterial agent, which is applied in Paecilomyces cicadae and its application field, and achieves the effect of good application prospect

Active Publication Date: 2018-07-24
HANGZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, most of the bacteriostatic agents used in the market are chemical agents, which can cause certain harm to the human body and the environment. However, biol...

Method used

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  • Paecilomyces cicadae and application
  • Paecilomyces cicadae and application
  • Paecilomyces cicadae and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 strain isolation and purification and identification

[0031] (1) Operation

[0032] 1. Strain isolation and screening

[0033] Clean the surface of the collected fresh cicadae, soak in mercuric chloride aqueous solution with a volume concentration of 0.10% for 1 min, soak in an aqueous solution of ethanol with a volume concentration of 75% for 1 min for disinfection, blot dry with sterile filter paper, and separate the tissues with a sterile scalpel. Inoculate on potato dextrose agar (PDA) plates (containing 10 μg / mL chloramphenicol), and culture at 28°C for 5 days. The cultivated cicadae samples were separated by streaking and coating methods, and strains of different shapes were picked and inoculated on new PDA plates, cultured at 28°C for 3 days, and pure strains were obtained by continuous separation for 3 times. After expanded culture, the bacteria were inoculated in PDA liquid medium, fermented at 28°C and 160rpm for 5 days, and the fermentation metab...

Embodiment 2

[0068] The preparation of embodiment 2 Paecilomyces cicadae CH113.1 bacteriostatic agent

[0069] (1) Slant culture: Paecilomyces cicadae CH 113.1 was inoculated into the slant medium, cultured at 28° C. for 5 days, and the slant cells were obtained. The slant medium is a PDA solid medium, and its composition is the same as in Example 1.

[0070] (2) Fermentation culture: pick an inoculation loop of bacteria from the slant and inoculate it into the seed medium, cultivate at 28° C. and 200 rpm for 5 days to obtain the seed liquid; the seed medium is a PDA liquid medium, the composition of which is the same as in Example 1.

[0071] (3) The seed solution was transferred to the fermentation medium with an inoculum volume concentration of 5.0%, and fermented at 28° C. and 200 rpm for 5 days to obtain a fermentation broth. Fermentation medium is PDA liquid medium, and composition is the same as embodiment 1.

[0072](4) Bacteriostatic agent: Paecilomyces cicadae CH 113.1 fermente...

Embodiment 3

[0073] The antibacterial performance of embodiment 3 Paecilomyces cicadae CH113.1 bacteriostatic agent

[0074] Escherichia coli, Bacillus cereus, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Enterococcus faecalis, Pseudomonas aeruginosa, Klebsiella pneumoniae, Salmonella paratyphi B, Candida albicans, Streptococcus Cortella sp., Penicillium expanse and Aspergillus niger were used as indicator bacteria, and the antibacterial activity of Paecilomyces cicadae CH 113.1 bacteriostatic agent was determined by Oxford cup method. Take OD 600 Prepare the indicator bacterium LB plate for the indicator bacteria liquid of 0.80% by volume concentration 2.0%, the Oxford cup is placed on the plate evenly horizontally, and the experimental group adds 200 μ L of Paecilomyces cicadae CH 113.1 bacteriostatic agent prepared in Example 2 Sterile aqueous solution, after standing at 4°C for 2 hours, cultured overnight at 37°C, measured the diameter of the inhibition z...

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Abstract

The invention discloses paecilomyces cicadae and application in preparing an antibacterial agent, a probiotic preparation and an antibacterial fresh-keeping film. A novel strain-paecilomyces cicadae CH 113.1 is first separated from field entomogenous fungus cordyceps sobolifera. A strain fermentation product has an inhibition effect for conventional pathogenic microorganisms, the inhibition rate for the escherichia coli can reach 70 percent or more, and the biological bacteriostatic agent can be applied to the fresh keeping of food. The strain has a good application prospect in the field of food.

Description

(1) Technical field [0001] The invention relates to Paecilomyces cicadae and its application as a bacteriostatic agent. (2) Background technology [0002] Pathogenic bacteria refer to microorganisms that can cause diseases, mainly including bacteria, viruses, spirochetes, rickettsia, chlamydia, mycoplasma, fungi, and actinomycetes. Generally speaking, pathogenic bacteria refer to bacteria in pathogenic microorganisms. The pathogenicity of bacteria is related to its virulence, invasion quantity and invasion portal. Although the vast majority of bacteria are harmless or even beneficial, a considerable portion can cause disease, thereby causing harm to the human body. Bacteriostats are substances that inhibit the growth of bacteria or fungi. A bacteriostatic agent may not kill the bacteria, but it can inhibit its growth, preventing harmful bacteria or fungi from overgrowing and endangering health. Commonly used antibacterial agents (Bacteriostat) are some antibiotic substan...

Claims

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Application Information

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IPC IPC(8): C12N1/14A23L3/3571C08L5/06C08K5/00C08K5/053C08J5/18C12R1/79
CPCA23L3/3571C08J5/18C08J2305/06C08K5/0058C08K5/053C12N1/145C12R2001/79Y02A40/90
Inventor 石陆娥张瑜唐振兴
Owner HANGZHOU NORMAL UNIVERSITY
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