Culture method of selenium-enriched cordyceps sobolifera conidial powder

Abstract

The invention discloses a culture method of selenium-enriched cordyceps sobolifera conidial powder. Firstly, separation, purification and identification are performed on cordyceps sobolifera collected in a field to obtain Paecilomyces cicadae (Miquel.) Samson, plate cultivation is performed to obtain single colonies, then, the single colonies are transferred to a liquid medium for culture, a liquid secondary seed solution is obtained, the secondary seed solution is transferred to a composite culture medium containing organic selenium fertilizer, the temperature is controlled to 15-35 DEG C, the humidity is controlled to 60-90%, ventilation culture is performed for 4-6 weeks, and harvesting is performed to obtain selenium-enriched cordyceps sobolifera conidial powder. Artificial simulation is performed to obtain nutritional and growth conditions closest to the conditions needed by cordyceps sobolifera wild environment, growth of cordyceps sobolifera is not limited by season changes, the composite culture medium is closer to natural bamboo forest cordyceps sobolifera wild environment, the breathability is good, the water absorption and moisturizing performance are excellent, a fruiting body grows rapidly, spore head and conidial powder of the fruiting body are abundant, thick, solid, full, large in conidial powder amount, high in quality and easy to harvest, the content of selenium is 20-100 mg / Kg, the method is simple and low in cost, the spore harvesting period is short, and industrial production can be achieved.

Description

technical field [0001] The invention belongs to the field of biotechnology. More specifically, it relates to a method for cultivating selenium-enriched cicada spore powder. Background technique [0002] Cicada is a complex of insects and fungi formed by Paecilomyces cicadae parasitizing on cicada nymphs in the genus Cicadae of the genus Cicadae in the family Ergotaceae. It is rich in nutrients and active ingredients. Documents and ancient books record that cicada has the effects of dispersing wind-heat, calming convulsions, and improving eyesight. It is used to treat children with sky hangs, night crying and heart palpitations, dispelling wind and spasm, measles, red eyes, and excessive tears. The medicinal part is the whole plant of cicadae. Cicada is composed of three parts: sclerotium, spore bundle and spore powder. Most of the literature pharmacological research reports and clinical experience are used as medicine or research on the whole plant. According to modern ph...

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved in the present invention is to overcome the deficiency of existing cicadae flower utilization and the defect of cicadae spore powder artificial cultivation technology, to provide a kind of compound culture medium that utilizes fungus Paecilomyces cicadae and organic selenium fertilizer to artificially cultivate cicadae flower and The method for obtaining selenium-enriched cicadae spore powder, which uses the environment in which wild cicadae grows, artificially simulates the nutrition and growth conditions required by the wild environment, so that the growth of cicadae is not restricted by seasonal changes, the method is simple and It is easy to cultivate and harvest on a large scale, and the harvesting amount of selenium-enriched spore powder of cicadae is much higher than that of wild cicadae spore powder

Images