258 results about "Pearl shell" patented technology

The invention provides a method of producing pearls on the basis of pearl shell mantel cell culture. The method includes: cutting mantel of a pearl shell into small slices, scattering to obtain single cells by enzymatic depolymerization, performing primary culture prior to subculturing, centrifuging, preparing solution by obtained cells, quantitatively injecting connective tissue in pearl shell mantel with the solution, placing the pearl shells in a culture area, and culturing for one cycle to obtain pearls.

The present invention relates to the growing method of pearl, especially color pearl. Before nucleus transplanting operation of pearl shell, pearl nucleus and transplanted cell piece are treated biologically with rose and other colored animal or plant nutriment; and after further cell piece transplantation and pearl transplanting, the pearl shell is cultivated in sea water to produce color pearl through cell fluid secretion and transmission. It has the beneficial effects of producing color pearl with bright and fast color via the simple biological process.

The invention discloses an in-vitro culture method for the pearl shell mantle tissue and a special culture solution thereof. The culture medium comprises hydrolyzed pearl liquor, shell liquor, antibiotics and seawater. The method conventionally comprises the following steps: taking the material from a nacre to obtain the mantle epithelium, removing the skirt band part, cutting the tissue into tissue blocks with the size of about 1mm to 2mm after purification treatment, putting the tissue blocks in the protease solution for digestion, and adding the culture solution to stop the digestion. The preliminarily digested tissue blocks are respectively put into a culture dish or cling to the pearl core which is covered by adherence substance. The cultured pearl shell mantle tissue grows in good conditions; after 4 hours, the cells are emigrated from the tissue blocks; and after 2 days in-vitro pearl sacs are formed effectively. The method can be used to inject the anucleate pearl sacs at different positions in the pearl shell with the inserted pearl nucleus so as to culture the anucleate pearl. The method has low cost, a high survival rate, a high pearl formation rate, and little damage to the nacre.

The invention relates to a Pteria penguin one-body two-use high efficient pearl cultivation method. Firstly, a core feeder is used for implanting a pearl core used for cultivating a dissociated pearl in the visceral sac of the Pteria penguin which is cultivated well; then the Pteria penguin in which the pearl core is implanted is put back to the culture water area for cultivating for a period of time; and a molding core which is used for cultivating blister pearl is implanted under the pallium of the Pteria penguin; the Pteria penguin in which the cores are implanted is put back to the culture water area for pearl cultivation; after the mature of pearl cultivation, the dissociated pearl is harvested from the visceral sac of the Pteria penguin and the blister molding pearl is harvested under the pallium. In the invention, as the one-body two-use high efficient pearl cultivation method for cultivating the dissociated pearl by implanting the core in the visceral sac and cultivating the blister pearl by implanting the core under the pallium in the visceral sac of the Pteria penguin by twice core implanting is adopted, the yield of pearl cultivation is improved efficiently, and the economic benefit is high. As the core feeder which consists of an outer tube, a core feeding needle and an adjustment device is adopted, the core implanting is safer, the orientation is more correct, and the working efficiency is high.

The invention discloses a treating process for preparing ceramic materials by pearl shells and wasted pearl bodies, and simultaneously further discloses a formula and a sintering technology for preparing daily-used ceramic and glaze by powder materials prepared by the treating process. The formula of shell porcelain body is as follows: 17-30 percent of mineral powder prepared by treatment, 10-20 percent of porcelain clay, 45-58 percent of kaoline, 0-5 percent of quartz, and 5-15 percent of feldspar; the formula of glaze of shell porcelain body is as follows: 1.36-4 percent of mineral powder prepared by treatment, 0,8-4 percent of porcelain clay, 3.6-11.6 percent of kaoline, 0-1 percent of quartz, 0.4-3 percent of feldspar, and 80-92 percent of high-boron high-zinc lead-free transparent frit; and the sintering technology of the shell porcelain is as follows: 1170-1220 DEG C of high-temperature plain temperature, and 1050-1100 DEG C of low-temperature glaze firing temperature. The invention has the advantages of energy conservation and consumption reduction, safety and environmental protection, and economy and practicalness.

The invention relates to a breeding method for a rapid growing line of seawater pearl shells, which comprises the steps of: taking a certain population of Pinctada martensii Dunker as a basic population, firstly selecting individuals with shell width being 10-40 percent higher than average value from the basis population by taking shell width as a selection index, then selecting individuals with shell height or weight being 10-60 percent higher than average value as breeding parents, dissecting the breeding parents, taking sexual glands to conduct artificial insemination, selecting and reserving preparative parents at a young shell stage according to a proportion of 20-30 percent, and finally conducting more than three generations of continuous selection to obtain the rapid-growing large excellent Pinctada martensii Dunker line (variety). The breeding method has the advantages that the method is simple, convenient and feasible, the cost for nursing the parents can be reduced, the growth speed and the individual size of the Pinctada martensii Dunker can be effectively improved and the pearl producing effect is improved.

The invention relates to a lift long line windproof culturing method of pearl shell. A series of upright main lines are fixed by floats and weights in the culturing area and slip rings are arranged on the main lines. The slip rings are connected with each other by long lines and a pull rope is arranged on the slip ring. The whole pull rope and the pearl shells cultured on the pull rope can be risen or dropped under the action of pulling the pull rope upwards or the gravity actions of slip rings, pull rope and the pearl shells cultured on the pull rope. The windproof function can be achieved when the long lines drop down to the underlayer of the water body and the culturing management operations can be carried out when the long lines rise up to the surface layer of the water body.

The invention discloses a pearl nucleus active liquid used for saltwater pearl culture and a preparation method thereof. In the pearl culture process, by using drug-treated pearl nuclei, immunological rejection on the pearl nuclei after a pearl shell nucleus-implanting operation can be reduced, the migration and the division of small cells are irritated, the formation of pearl sacs is promoted, the wound infection after the operation is reduced, and the goal of comprehensively improving the pearl quality and yield by reducing the nucleus-spitting rate, increasing the thickness of pearl layers and improving the survival rate of cultured pearls is achieved. In the invention, the pearl nucleus active liquid is prepared by utilizing glacial acetic acid, chitosan, tetracycline antibiotics and sterilized filtered seawater. When the pearl nuclei treated by the invention are used for pearl culture, the survival rate of the cultured pearls is increased by 2.59 to 7.33 percent, the pearl-containing rate is increased by 9.8 to 20.07 percent, and the high-quality pearl rate is increased by 5.97 to 12.55 percent.

A method capable of promoting stripped shellfish eggs to be mature in vitro is characterized by first stripping off mature shellfish to obtain eggs and sperm, filtering and washing the eggs after stripping, then washing the eggs after being treated by ammonia water at a concentration of 0.005-0.01% for 10-15min and then by 5-hydroxytryptamine at a concentration of 0.001-0.002M for 30-45min, finishing artificial fertilization after combination of the eggs and sperm, which are promoted to be mature in vitro and enabling fertilized eggs to hatch and grow to normal D-type larvae. The shellfish is abalone, bloody clams, sinonovacula constricta, clams, pearl shells or mussels. In the method, the complementary actions of the ammonia water and 5-hydroxytryptamine are adopted, therefore, the respective side effects of the ammonia water and 5-hydroxytryptamine are reduced, the fertilization rate and hatchability are improved and the deformity is less. The method is simple in operation and can be applied to seedling production.

The invention discloses a factory-like pearl culture system. With worsening of seaculture environments, seashore benches and neritic regions are no longer the only place for pearl culture, and factory-like pearl culture is a good choice. The factory-like pearl culture system is composed of a water pump house, an algal culture pond, a seawater filtering tank, a fresh water filtering tank and a pearl culture pond. Pearl shell seedlings are put in the pearl culture pond, filtered seawater according with seawater salinity, power of hydrogen and temperatures is injected into the pearl culture pond through a pipeline, seawater with algae is also injected into the pearl culture pond through a pipeline, and the algae are used for pearl shell culture. The factory-like pearl culture system integrates scientific research, ecology, culture and demonstration, factory-like pearl culture fundamentally solves the problem of environmental pollution in sea farming in the prior art and is free from influences of natural environment, tests show that the survival rate of pearls cultured through the factory-like pearl culture system reaches more than 95%, the rate of finished products also reaches more than 95%, and the factory-like pearl culture system represents one development direction of pearl culture.

The invention discloses a pearl cultivating method for nuclear pearl, a tool adopting the method and cultivated pearl. The pearl cultivating method for nuclear pearl comprises the following steps: nuclear-free pearl is used as a pearl nucleus or a shell is grinded to form the pearl nucleus; the pearl nucleus is arranged on a cell sheet and then the pearl nucleus with the cell sheet is implanted on viscera or pelecypodium of a mussel or a pearl shell with a tool; the mussel and the pearl shell is restored in water for culture; the mussel or the pearl shell are opened and a pearl formed around the pearl nucleus is harvested. As the pelecypodium of the mussel or the pearl shell is above a visceral sac and has large space compared with the visceral sac or a mantle, and the pelecypodium secretes more nacre, the pearl nucleus cannot be spit out easily if the pearl nucleus is implanted on the pelecypodium and the diameter of the pearl nucleus is expanded, moreover, the mussel or the pearl shell secretes more nacre after the pearl nucleus is coated. The pearl cultivating method ensures that the pearl nucleus can be inserted into the pelecypodium accurately, the cell sheet can wrap the pearl nucleus better and the pearl nucleus has no need of punching a hole. The pearl has large particle, good translucent luster, short growth cycle and high economic value.

The invention discloses a traditional Chinese medicine composition for treating a heart-deficiency timidity disease and a preparation method thereof. The traditional Chinese medicine composition comprises the following raw materials: radix pseudostellariae, ginseng, American ginseng, raw milkvetch root, yam, white peony, radix codonopsitis, pilose antler, aweto, bitter cardamon, prepared rhizome of rehmannia, angelica, root of straight ladybell, polygonatum, sealwort, fruits of glossy privet, turtle shells, tortoise plastron, shiandra, amber, storax, acorus gramineous, senso, borneol, muskiness, shelled cedar seed, polygala, ormer, pearl shell and uteroglobin. The traditional Chinese medicine composition can be prepared into any common oral preparation according to a general traditional Chinese medicine composition method, can remarkably eliminate the symptoms, such as palpitation and short breath, qi deficiency, chest distress, funk restless, timidity insomnia, night terror dreaminess, qi deficiency hypodynamia, spontaneous perspiration night sweating, arrhythmia, and the like, have demanded curative effect and remarkable curative effect and rapid action, basically adopt medicaments stipulated by the national pharmacopoeia for the homology of medicine and food for composition and have the advantages of low expense, basic toxic and side effect prevention, and the like.

The invention relates to the field of oral cleaning, and particularly relates to dendrobium officinale toothpaste with an oral repair function and a preparation technology of the dendrobium officinale toothpaste. The dendrobium officinale toothpaste with the oral repair function is at least prepared from the following components: 0.1-1.0 part of first dendrobium officinale extract, 1.0-10 parts of bletilla extract, 15.0-35.0 parts of sorbitol, 8.0-25.0 parts of hydrated silica, 5.0-12.0 parts of glycerinum, 1.0-3.0 parts of borneol, 1.0-6.0 parts of pearl shell powder, 0.5-3.0 parts of sodium lauryl sulfate, 1.0-10.0 parts of polyethylene glycol, 0.5-3.0 parts of xylitol, 0.3-1.0 part of sodium carboxymethylcellulose, 1.0-1.0 part of chondrus crispus, 0.1-1.0 part of xanthan gum, 3.0-8.0 parts of saccharin sodium, 1.0-3.0 parts of C119140, 1.0-3.0 parts of C142090 and 10-40 parts of water.

The intermediate net-cage culturing process of artificial pearl shell fry includes setting floated net cage with net coating inside gulf sea area, covering with two layers of shading net in 95 % transmittance, attaching pearl shell fry onto transverse rope, replacing net coating and collecting fallen live fry and culturing the pearl shell fry to 25-30 mm size. The present invention has high survival rate.

The invention relates to a traditional Chinese medicine formula for treating myopia, which comprises the following materials: a main material contains 10-30g of cassia seed, 5-10g of concha haliotidis, 30-50g of chrysanthemum, 2-5g of celosia seed, 2-5g of prunella vulgaris, 10-30g of mulberry leaf, 5-10g of pearl shell, 5-10g of mint, 10-30g of wolfberry and 2-5g of Radix Rehmanniae Preparata; and an auxiliary material of 1500-3000g of distilled water. A manufacturing method comprises the following steps: blending the above components, mashing, and placing in a container filled with water, and boiling and adjusting the firepower smaller. The steam of the traditional Chinese medicine formula is used for fumigating the eyes, each time lasts 30 minutes for twice, and the traditional Chinese medicine formula has efficacy for treating 80% of myopia.

The present invention belongs to the field of food technology, and is especially mussel protein product and its production process. The present invention provides one new type of protein product with the mussel clam as the side product of freshwater pearl shell as the main material and through alkali dissolution and acid precipitation. The production process includes leaching mussel clam in dilute alkali solution for 1-24 hr, centrifuging to obtain supernatant, regulating pH of the supernatant to isoelectric point to deposit protein, centrifuging separation to take precipitant, and drying to obtain mussel protein product. The mussel protein product is light yellow powder with protein content over 90 % and excellent functional characteristic, and may be used in food production.

A shellfish engineering culture method is characterized in that the shellfish engineering culture method comprises the following steps of: (1) arranging culture carriers of multilayer structures in a culture space; (2) Layering and tiling shellfishes in the culture carriers of the multilayer structures; and (3) drawing seawater to feed into the culture space as culture water of the shellfishes, wherein the culture water flows out of the culture space after passing through the culture carriers of the multilayer structures from bottom to top, and the shellfishes on the culture carriers of the multilayer structures obtain natural bait in the rising culture water as food layer by layer. Living bait in a natural sea area is fully utilized, the seawater is drawn into the semi-enclosed culture space by a water pump, the culture space is designed in a rising flowing water mode, the culture water flows out of the culture space after passing through the culture carriers of the multilayer structures from bottom to top, and the shellfishes on the culture carriers of the multilayer structures obtain the natural bait in the rising culture water as food layer by layer. According to the shellfish engineering culture method, utilization rate of the natural bait is largely improved, engineering high-density culture of the shellfishes such as pearl shells is achieved, and culture benefit is increased.

The invention discloses a traditional Chinese medicine feed for raising broiler chickens and a preparation method of the traditional Chinese medicine feed. The traditional Chinese medicine feed comprises a traditional Chinese medicine additive and a high-protein feed body, wherein the traditional Chinese medicine additive part comprises the following components and raw materials in parts by weight: 40-50 parts of calculus bovis syntheticus, 11-15 parts of eucommia ulmoides, 4-8 parts of asparagus cochinchinensis, 1-3 parts of medicine terminalia fruits, 7-10 parts of fleeceflower roots, 4-6 parts of pearl shells, 5-8 parts of scutellaria baicalensis, 8-10 parts of honeysuckles, 11-15 parts of bighead atractylodes rhizomes and 4-5 parts of Wei stones. Through the traditional Chinese medicine additive, the endocrine systems of the broiler chickens are changed, the immunity of the broiler chickens is strengthened, and the phenomenon that the meat quality of the broiler chickens is pale and flavorless can be effectively avoided; besides, the high-protein feed is matched with the traditional Chinese medicine additive, so that the content of proteins in broiler chicken eggs is promoted; besides, coarse materials, a beneficial viable bacterium preparation and a beneficial enzyme preparation are mixed, so that the craw of the broiler chickens generates peristalsis, and the decomposition and the absorption of proteins are promoted.

The invention discloses pearl calcium ion particles and a production method thereof. 8 to 12 weight percent of pearl, 58 to 62 weight percent of coral, 8 to 12 weight percent of pearl shell, 8 to 12 weight percent of shrimp shell, and 8 to 12 weight percent of crab shell are evenly mixed according to the proportion, are stirred, are calcined and dried for 12 hours at high temperature of between 1,100 and 1,250 DEG C to remove unnecessary impurities, and then are cooled; then the sintered substance is ground, is then placed into a container for oxidation, and then is cooled to obtain finished pearl calcium ion powder; in addition, the powder is mixed with pottery clay for pelleting, calcination and temperature reduction to finish another finished product, namely the pearl calcium ion particles, thereby ensuring that calcium ion components can be released and alkaline calcium ion water can be obtained when the powder or the particles containing 85 to 95 percent of pearl calcium ions are placed into water.

The invention relates to the field of ceramic material, and particularly relates to a preparation method of highly oriented nanometer MAX phase ceramics and MAX phase in-situ synthesized oxide nanometer composite ceramics. Nanometer lamellar powder of the MAX phase ceramics or an embryo formed by the nanometer lamellar powder is adopted as the material, the granularity of nanometer lamellar particles of the MAX phase ceramics in the powder or the embryo is between 20-400 nanometers, and the oxygen content is between 0.0001%-20% mass fraction; MAX phase grains in ceramics obtained after sintering of the material is lamellar or cambiform, and lamellar grains has high orientation. According to the invention, orientation is generated by pressurized deformation by utilizing the special characteristic of the nanometer lamellar MAX powder, so as to obtain a lamellar structure similar to that in a natural pearl shell; such structure, like brick fragments used for building, has very high forcebearing capacity and resistance to external load and crack expansion.

The invention provides a folium apocyni veneti oral liquid for preventing and curing palpitaition, insomnia and neurasthenia. The folium apocyni veneti oral liquid contains the following raw materials, by weight, 10-50 parts of folium apocyni veneti, 10-20 parts of uncaria, 10-20 parts of polygala root, 10-20 parts of poria with hostwood, 10-20 parts of arborvitae seed, 10-20 parts of spine date seed, 10-20 parts of red sage root, 10-20 parts of Ligusticum wallichii, 10-20 parts of corydalis tuber, 10-20 parts of epimeddium, 10-20 parts of polygonatum rhizome, 10-20 parts of Radix Polygoni Multiflori Preparata, 10-20 parts of ligustrum lucidum, 10-20 parts of Chinese magnoliavine, 10-20 parts of cortex lycii, 10-20 parts of pearl shell, 10-20 parts of calcined oyster shell, 10-20 parts of white peony root, 10-20 parts of periostracum cicadae, 10-20 parts of cherokee rose-hip and 10-20 parts of albizia bark. The oral liquid provided by the invention has functions of strengthening and nourishing marrow and essence, reinforcing qi, nourishing blood, strengthening brain and tranquilizing mind, is used for kidney essence deficiency, dizziness caused by deficiency of both qi and blood, hypodynamia, forgetfulness and insomnia and can greatly achieve the effect of improving neurasthenia as for neurasthenia patients with the above symptoms.

The present invention relates to a method for non-destructive judgment of pearl quality by measuring the ultraviolet-visible reflectance spectrum and / or ultraviolet-visible fluorescence spectrum of a pearl or pearl shell to be inspected and comparing the values obtained with those preliminarily measured for a normal pearl or pearl shell. The present invention also relates to a non-destructive inspection apparatus of pearl quality. According to the present invention, the quality of the aimed pearl or pearl shell can be easily and rapidly judged under a non-destructive condition.

A pharmaceutical composition for treating headache, prepared from eleven Chinese herbal medicines: Chinese angelica root, ligusticum chuanxiong, radix paeoniae lactiflorae, prepared rhizome of rehmannia, uncaria tomentosa, leatherleaf milletia, prunella vulgaris, sicklesenna seed, pearl shell, corydalis tuber, and asarum, and a proper amount of adjuvant materials. A preparation method of the pharmaceutical composition, and uses thereof in the preparation of drugs for treating various headaches, traumatic cranial nerve syndrome, dizziness and vertigo, vexation and irritability, insomnia and dreaminess.

The invention discloses a forced nucleus retention method in nucleated pearl cultivation and a nucleus retainer used in the method. The nucleus retainer comprises a nucleus retaining bar and a base, wherein the nucleus retaining bar is used for retaining a nucleus, and the base is used for fastening the nucleus retaining bar to a pearl shell. The method includes: fastening the base to the edge of the pearl shell through bolts or fastening the base to the outer side of the pearl shell in an adhered manner, extending one end of the nucleus retaining bar into an outer holding cavity of the pearl shell, exerting certain pressure to hold the nucleus retaining bar onto the outer side of a visceral sac at the position of the nucleus, and fastening the other end of the nucleus retaining bar onto the base; placing the pearl shell in culture water for 10 to 15 days of temporary culture, and removing the nucleus retainer; and forcedly retaining the nucleus which is implanted and has a wound healed in the pearl shell without 'exposure'. The forced nucleus retention method and the nucleus retainer are effective in nucleus fixation, nucleus retention rate of nucleus implanted shells can reach 100% under experimental conditions, and the problem that a nucleus of a large nucleated pearl is 'exposed' during culturing is solved.

The invention relates to a traditional Chinese medicine for treating the insomnia, which is prepared by the following active pharmaceutical ingredients by weight: 5-10g of cortex albiziae, 3-5g of shelled cedar seed, 10-20g of dragon bone fossilized powder, 6-12g of radix astragali, 8-15g of pearl shell, 3-5g of radix salviae miltiorrhizae, 3-5g of flower bud of Japanese apricot, 4-6g of liquorice, 4-8g of white atractylodes rhizome, 5-10g of tuckahoe, 2-5g of cortex moutan, 2-4g of radix paeoniae alba and 8-10g of bupleurum chinense. The traditional Chinese medicine for treating the insomnia disclosed by the invention can treat the insomnia caused by various symptoms, and has lower cost and no toxic or side effect. In the invention, the clinically effective rate is 80%, and the curative ratio is 75%.

The invention relates to a traditional Chinese medicinal composition for treating retrograde menstruation. The traditional Chinese medicinal composition is prepared from the following active pharmaceutical ingredients: by weight, 15-25 parts of pearl shell, 12-18 parts of dried rehmannia root, 12-18 parts of Radix Astragali, 12-18 parts of madder, 12-18 parts of radix paeoniae rubrathe, 12-18 parts of Cyperus Rotundus L, 12-18 parts of motherwort, 12-18 parts of imperata rhizome, 10-15 parts of yerbadetajo herb, 8-12 parts of Chinese angelica, 8-12 parts of Radix Codonopsis, 8-12 parts of red sage root, 4-8 parts of achyranthes root, 4-8 parts of cape jasmine, 4-8 parts of tree peony bark, 4-8 parts of coptis, 4-8 parts of safflower, 4-8 parts of licorice and 2-4 parts of radix bupleuri. The traditional Chinese medicinal composition provided by the invention has advantages of stable curative effect, little side-effect and low cost.

The invention discloses an intractable insomnia treatment medicine. The intractable insomnia treatment medicine is prepared from 30-120 g of spine date seeds, 10-20 g of Flos Albiziae, 10-20 g of rhizoma gastrodiae, 10-20 g of Radix Polygalae, 10-20 g of Acorus gramineus, 10-100 g of keels, 10-100 g of oysters, 10-60 g of magnets, 15-40 g of pearl shells, 10-40 g of Caulis Polygoni Multiflori, 10-30 g of platycladi seeds, 10-20 g of Poria cum Ligno Hospite, 10-50 g of Dens Draconis and 10-20 g of Chinese magnoliavine. The medicine has scientific and reasonable compatibility, and above medicines have synergism to realize the efficacy of eliminating phlegm and inducing resuscitation, nourishing the liver and the kidney, and nourishing the heart and tranquilizing mind, so the medicine has an extremely treatment effect on insomnia caused by various reasons; and additionally, the medicine has the advantages of high curative effect, fast effect, low cost, convenience in taking, good safety and no side effects.

The invention discloses a pearl shell nucleus insertion pearl culture worktable which comprises a rectangular worktable body, a base, a flexible small spot lamp, a water tank, a connecting water pipe and an object placing frame. Baffle plates are arranged at the front edge, the left edge and the right edge of the rectangular worktable body respectively, and an operating frame is fixed on the base. Each worktable is used by one nucleus insertion worker, various tools needed by nucleus insertion operations can be cleaned and stored on the worktable body, the whole nucleus insertion operation is more ordered, working efficiency is improved, the small spot lamp is arranged on the worktable and can be integrally bent, the workers can adjust a lamp holder, so that light of the lamp irradiates into an opened pearl culture shell, the nucleus insertion operation can be performed in all places and at any time and cannot be affected by weather and environments, the worktable can be randomly moved and placed and is matched with a temporary culture device and a delivery route of the pearl culture shell, so that the nucleus insertion operation is more scientific and ordered, and the worktable has a fine application prospect.

The invention provides a method of culturing pearls by using pearl nucleuses with tissue engineering scaffolds. The method is characterized in that a layer of degradable high-molecular polymer with a uniform thickness, serving as a tissue engineering scaffold, is attached to the surface of a pearl nucleus which is to be implanted into a pearl mussel; the tissue engineering scaffold is three-dimensional network space capable of accommodating and growing cells; mantle tissue of pearl shells is separated into single existent free mantle cells by using the enzyme digestion method and a mantle cell suspension with a certain cell density is prepared; mantle cells are attached to the pearl nucleus and then the pearl nucleus and the mantle cell suspension are implanted in a nucleus insertion position of the pearl mussel for culture of a nucleus pearl. The method is simple in operation, requires no in-vitro culture of cells and solves the problem of high technology and equipment requirements of a cell culture method; aseptically packaged pearl nucleuses can be directly transported to culture sites for operation and the transportation is convenient; the method has low requirements for equipment and operators, reduces the production cost greatly and is suitable for large-scale production and application.

The invention relates to a method for culturing nucleated pearls by using magnetic attraction fixed pearl nucleuses. The method comprises the following steps of: implanting magnetic pearl nucleuses and mantle cell small pieces into nuclear potential positions of pearl shells or (clams) by the conventional nucleus implanting surgical method; placing nucleus fixing magnets outside visceral sacs or mantles at the positions of the pearl nucleuses, wherein the magnets stretch magnetic attraction generated by the magnetic pearl nucleuses by isolating from the epidermal tissues of the visceral sacs or the mantles to prevent the pearl nucleuses from being protruded out from cuts through which the pearl nucleuses are implanted; placing the shells (or the clams) implanted with the nucleuses into a culturing water area, revitalizing for 10 to 20 days, and opening the shells (or the clams) implanted with the nucleuses by a shade-drying method and the like to take the nucleus fixing magnets out after the surgical wounds of the shells (or the clams) implanted with the nucleus are basically healed; and placing the shells (or the clams) implanted with the nucleuses into the culturing water area, and culturing for a certain time to form the magnetic nucleated pearls. By the method for culturing the nucleated pearls, the nucleus implanting and keeping rate can be effectively improved by more than 90 percent, and spherical, heteromorphic and image nucleated pearls with a magnetic health care effect can be produced.