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Solid medium for producing plasmin by using cordyceps sobolifera and plasmin production method using same

A technology of solid medium and plasmin, which is applied in the field of microorganisms, can solve the problems that Paecilomyces cicadae has not been reported, and achieve the effect of easy preparation and simple formula

Inactive Publication Date: 2016-11-23
李伟
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although plasminase has been reported in other Cordyceps fungi such as Cordyceps sinensis and cordyceps militaris, it has not been reported in Paecilomyces cicadae

Method used

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  • Solid medium for producing plasmin by using cordyceps sobolifera and plasmin production method using same
  • Solid medium for producing plasmin by using cordyceps sobolifera and plasmin production method using same
  • Solid medium for producing plasmin by using cordyceps sobolifera and plasmin production method using same

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Experimental program
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Effect test

Embodiment 1

[0032] A culturing method (screening method) of a solid medium for producing plasmin by utilizing cicada flowers:

[0033] 1 Medium used

[0034] (1) Basic medium: 30 g of glucose, 1 g of potassium dihydrogen phosphate, 3 g of yeast extract, 0.2 g of magnesium sulfate 7hydrate, and 1000 g of water. 1000 g of water is equivalent to 1000 mL of water.

[0035] (2) Plasmin solid induction medium: Add 10g of the following different induction components to the basic medium: peptone, acid hydrolyzed casein, tryptone, starch, casein, Malt extract (malt extract), milk powder, soy peptone (Soytone), prepared into different components of plasmin induction medium. Finally, 1.5% agar was added to the above various media. Conventional high temperature sterilization (1.1 atmosphere pressure, sterilization at 121 ℃ for 20 minutes).

[0036] (3) Potato dextrose agar (PDA): Cut 200 g of potatoes into small pieces, add water to boil for 15 minutes, then filter with 2 layers of gauze, discard...

Embodiment 2

[0060] Example 2: qianrongm plasmin activity (U / mL)

[0061] Optimization of the induction components of solid medium for plasmin production by Cicada flower:

[0062] 1 Medium used

[0063] (4) Basic medium: 30 g of glucose, 1 g of potassium dihydrogen phosphate, 3 g of yeast extract, 0.2 g of magnesium sulfate 7-hydrate, and 1000 g of water. 1000 g of water is equivalent to 1000 mL of water.

[0064] (5) Plasmin solid induction medium: add peptone and tryptone to the basic medium, respectively. The test content gradient of peptone is 7, which are 5g, 7.5g, 10g, 12.5g, 15g, 17.5g, 20g; the test content gradient of tryptone is 7, which are 5g and 7.5g respectively. , 10g, 12.5g, 15g, 17.5g, 20g. The plasmin-inducing medium with different components was prepared according to the above components. Finally, 1.5% agar was added to the above various media. Conventional high temperature sterilization (1.1 atmosphere pressure, sterilization at 121 ℃ for 20 minutes).

[0065] (...

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Abstract

The invention discloses a solid medium for producing plasmin by using cordyceps sobolifera and a plasmin production method using the same, belonging to the field of microbial technology. The solid medium comprises the following active ingredients in parts by weight: 8-15 parts of peptone, 30 parts of glucose, 1 part of monopotassium phosphate, 3 parts of yeast extract, 0.2 part of magnesium sulfate heptahydrate, 15g of agar and 1000 parts of water. A plasmin production method using the solid medium for producing plasmin by using cordyceps sobolifera comprises the following steps: (1) inoculating a paecilomyces cicadidae strain (B5) to a PDA panel and culturing; (2) sterilizing the prepared solid medium and pouring into a sterilized petri dish; cooling to obtain a solid panel; teasing the hypha block with a teasing needle, inoculating to the prepared solid inducing medium panel and culturing; and (3) putting the cultured bacterial colony in a refrigerator overnight; fetching the hypha block with medium and adding into sterile water; mashing and standing; performing high-speed centrifugal separation; and fetching the supernate as a crude extract of plasmin for later use.

Description

technical field [0001] The present invention relates to a solid culture medium for producing plasmin by utilizing Cicada flower and a method for producing plasmin, belonging to the technical field of microorganisms, and in particular to a solid culture medium and a culturing method for inducing Paecilomyces cicadae to produce plasmin. Background technique [0002] Thrombotic disease is a common disease with high fatality rate, mainly including cerebral thrombosis, pulmonary embolism, coronary blood, venous thromboembolism, atherosclerotic thrombosis, peripheral arterial thrombosis, etc., which seriously threaten human health. The main methods of treatment for thrombosis are surgical and drug removal. According to statistics, there are more than 15 million patients with various thrombotic diseases in the world, and this disease is developing towards a younger age, requiring a large amount of thrombolytic drugs every year. [0003] The current thrombolytic drugs are divided i...

Claims

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Application Information

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IPC IPC(8): C12N9/68C12R1/79
CPCC12N9/6435C12Y304/21007
Inventor 李伟
Owner 李伟
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