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Fermentation method of antioxidant cordyceps sobolifera mycelia

A fermentation method and mycelium technology are applied in the field of fermentation of anti-oxidative cicadae mycelium, which can solve the problems of environment pollution caused by fermented liquid discharge and low yield of mycelium, and achieve the advantages of simple process, easy control and increased sources. Effect

Pending Publication Date: 2020-01-14
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on the artificial fermentation of cicadae is mainly to explore the medium components and growth conditions of mycelium growth, but there are problems such as low mycelium production and environmental pollution caused by the discharge of fermentation broth.

Method used

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  • Fermentation method of antioxidant cordyceps sobolifera mycelia
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  • Fermentation method of antioxidant cordyceps sobolifera mycelia

Examples

Experimental program
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Effect test

Embodiment 1

[0033] (1) Culture medium preparation: prepare PDA slant medium, seed culture fluid and fermentation medium; Potatoes are boiled 15min, filter and get juice wherein, PDA slant medium contains the potato juice that massfraction accounts for 20% of medium total mass, accounts for Glucose at 2% of the total mass of the medium, agar powder accounting for 1.5% of the total mass of the medium, the solvent is water, the pH is natural, and sterilized at 121°C for 30 minutes; the seed culture solution contains sucrose with a mass fraction of 2% of the total mass of the medium, containing Soluble starch whose mass fraction accounts for 3% of the total mass of the medium, soybean powder with a mass fraction of 2% of the total mass of the medium, and KH with a mass fraction of 0.1% of the total mass of the medium 2 PO 4 , containing MgSO with a mass fraction of 0.05% of the total mass of the medium 4 ·7H 2 O, the solvent is water, and the pH is natural. The volume of liquid in the shak...

Embodiment 2

[0044] (1) Culture medium preparation: prepare PDA slant medium, seed culture liquid and fermentation medium; Potatoes are boiled 30min, filter and get juice wherein, the potato juice that mass fraction accounts for 20% of the total mass of medium is contained in PDA slant medium, accounts for Glucose at 2% of the total mass of the medium, agar powder accounting for 2% of the total mass of the medium, the solvent is water, the pH is natural, and sterilized at 121°C for 30 minutes; the seed culture solution contains sucrose with a mass fraction of 2% of the total mass of the medium, containing The mass fraction accounts for 3% of the total mass of the medium soluble starch, contains the mass fraction of 2% of the total mass of the medium for soybean powder, and contains the mass fraction of 0.1% of the total mass of the medium for KH 2 PO 4 , containing MgSO with a mass fraction of 0.05% of the total mass of the medium 4 ·7H 2 O, the solvent is water, and the pH is natural. ...

Embodiment 3

[0055] (1) Culture medium preparation: prepare PDA slant medium, seed culture liquid and fermentation medium; Potatoes are boiled 20min, filter and get juice wherein, the potato juice that mass fraction accounts for 20% of medium total mass is contained in PDA slant medium, accounts for Glucose at 2% of the total mass of the medium, agar powder accounting for 1.9% of the total mass of the medium, the solvent is water, the pH is natural, and sterilized at 121°C for 30 minutes; the seed culture solution contains sucrose with a mass fraction of 2% of the total mass of the medium, containing The mass fraction accounts for 3% of the total mass of the medium soluble starch, contains the mass fraction of 2% of the total mass of the medium for soybean powder, and contains the mass fraction of 0.1% of the total mass of the medium for KH 2 PO 4 , containing MgSO with a mass fraction of 0.05% of the total mass of the medium 4 ·7H 2 O, the solvent is water, and the pH is natural. The v...

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Abstract

The invention provides a fermentation method of antioxidant cordyceps sobolifera mycelia. The fermentation method comprises the following steps: respectively preparing a PDA slant culture medium, a seed culture solution and a fermentation culture medium; dividing the paecilomyces cicadae mother strain through sterile operation, and inoculating the paecilomyces cicadae mother strain to the PDA slant culture medium for activation culture to obtain an activated strain; inoculating the activated strain into a seed culture solution to obtain a primary strain; inoculating the primary strain into theseed culture solution, and culturing the primary strain according to a method for culturing the activated strain to obtain a secondary strain; inoculating the primary strain or the secondary strain into the seed culture solution for culturing to obtain a fermentation tank seed solution; inoculating the fermentation tank seed solution into the fermentation culture medium, and carrying out fermentation culture to obtain cordyceps sobolifera fermentation liquor; and discharging the cordyceps sobolifera fermentation liquor, and carrying out semi-solid fermentation culture to obtain the fermentation product of the antioxidant cordyceps sobolifera mycelia. The fermentation method is suitable for fermentation production of cordyceps sobolifera mycelia, the obtained mycelia is high in biomass andstrong in antioxidant activity, the yield of active substance polysaccharide and cordycepic acid can be remarkably increased, and the source of health-care food raw materials is increased.

Description

technical field [0001] The invention relates to a method for fermenting mycelia, in particular to a method for fermenting anti-oxidation cicada mycelium. Background technique [0002] Cicadae, or Cordyceps cicadae, is a fungus with both medicinal and edible uses, and it is also one of the traditional Chinese medicinal materials in my country. Cicada has a wide range of pharmacological activities. Cicada can enhance the immune regulation function of the body, nourish and strengthen the body, improve the nutritional status of the body, relieve heat and pain, improve kidney function, resist radiation, fatigue, stress, oxidation, and bacteriostasis , Anti-tumor, anti-virus, regulating lipid metabolism, lowering blood pressure, lowering blood sugar, anti-convulsant, etc., widely used in clinical practice. The ingredients and medicinal value of cicadae are comparable to those of Cordyceps sinensis, and have high edible, nutritional and medicinal values. It is considered to be one...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 凡军民杨雨靖曹正黄小忠郁宝锋宋小玉
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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