483 results about "Cordyceps sp." patented technology

The invention relates to a saccharomyces cerevisiae strain for efficiently synthesizing cordycepin as well as a construction method and application of the saccharomyces cerevisiae strain, and belongs to the technical field of microbial genetic engineering. The construction method provided by the invention comprises the following steps: carrying out multi-copy genome integrated expression on heterologous codon optimized 2 '-carbonyl-3'-deoxyadenosine reductase genes and 3 '-adenosine monophosphate phosphohydrolase genes, so as to increase the dosage of key enzymes; and supplementing the defective gene of the engineering strain to obtain the saccharomyces cerevisiae strain B5U3 with high yield of cordycepin. According to the obtained saccharomyces cerevisiae strain for efficiently synthesizing the cordycepin, the cordycepin yield reaches 2.92 g / L after shake flask fermentation, the cordycepin yield reaches 7.5 g / L after fermentation in a fermentation tank, and a foundation is laid for safer and more reliable industrial production of the cordycepin.

The invention relates to the technical field of microbial genetic engineering, in particular to a method for improving cordycepin synthesized by saccharomyces cerevisiae and saccharomyces cerevisiae engineering bacteria. According to the invention, codon-optimized 2 '-carbonyl-3'-deoxyadenosine reductase gene and 3 '-adenosine monophosphate phosphohydrolase gene from cordyceps militaris are expressed by using plasmids, and then the plasmids are transformed into host cells to obtain the saccharomyces cerevisiae engineering bacteria. And inoculating a seed solution obtained by seed culture of the activated saccharomyces cerevisiae engineering bacteria into a fermentation culture medium containing a metabolic effector, and carrying out fermentation culture to synthesize cordycepin. Metabolic effectors (Cu < 2 + >, Fe < 2 + >, Mg < 2 + >, Zn < 2 + >, citric acid, cysteine, aspartic acid, glycine, VB1 and tea polyphenol) are added into a fermentation culture medium strain, and the variety and concentration of the metabolic effectors are optimized, so that the yield and synthesis efficiency of cordycepin are remarkably improved. By using the combination of the strain obtained by the invention and the optimal effector, the yield of cordycepin in a fermentation tank reaches 2.9 g / L.

The invention provides a preparation method of a cordyceps militaris fermentation product with anti-aging, mitochondrial function enhancing, anti-oxidation and autophagy promoting effects, and the preparation method comprises the following steps: inoculating cordyceps militaris into a fermentation substrate for liquid fermentation culture to obtain a fermentation mycelium; the fermented mycelium is subjected to wall breaking or centrifugal separation, concentration and freeze-drying, and the cordyceps militaris fermented product is prepared; wherein the fermentation substrate comprises oat bran and / or lily. The cordyceps militaris fermentation product prepared by the method shows remarkable anti-aging, anti-oxidation, mitochondrial function enhancing and autophagy promoting effects in in-vitro cell experiments and in-vivo animal models, the process period is short, the preparation process is simple and easy, liquid fermentation in the whole process is suitable for large-scale production, and the preparation cost is low.

The invention relates to the technical field of data processing, in particular to a data processing method for component content detection.The method comprises the steps that a chromatogram of a cordyceps militaris extract is obtained through high performance liquid chromatography, the abscissa of the chromatogram is retention time, and the ordinate of the chromatogram is an absorbance value; and performing baseline correction on the chromatogram by using an ALS algorithm, in the correction process, adjusting the peak sensitivity of the ALS algorithm based on the adaptive weight of each data point in the chromatogram, and performing baseline correction so as to detect the cordycepin concentration in the cordyceps militaris extract based on the chromatogram after baseline correction. According to the method, the baseline correction accuracy of the chromatographic data of the cordyceps militaris extract can be improved, so that accurate detection of the concentration of cordycepin in cordyceps militaris is realized.

The invention provides cytokine hydrogel as well as a preparation method and application thereof in skin injury repair, and belongs to the technical field of biological medicines. Comprising the following steps: (1) adding beta-GP into a chitosan solution to obtain a CS-beta-GP pregel solution; (2) dissolving Pluronic F127 and hyaluronic acid in a PBS (Phosphate Buffer Solution) until the Pluronic F127 and the hyaluronic acid are completely transparent to obtain an F127-HA solution; and (3) sequentially dissolving trehalose, cordycepin, a cell factor composition and collagen peptide in a PBS solution, incubating to obtain a cell factor mixed solution, and mixing the cell factor mixed solution with the CS-beta-GP pregel solution and the F127-HA solution. According to the invention, a dual temperature-sensitive system is established, the gel can be quickly formed under the conditions of injection at low temperature and body temperature, the effective period of active ingredients is prolonged by utilizing a trehalose stabilization technology, and meanwhile, the anti-inflammatory effect of cordycepin and the cell repair effect of collagen peptide are combined, so that the efficient repair of skin injury can be realized.

The invention provides a skin-care and immunity-enhancing traditional Chinese medicine extract, milk powder and a preparation method, and relates to the technical field of food. The traditional Chinese medicine extract comprises the following components: red ginseng powder, cordyceps militaris powder, astragalus membranaceus powder, angelica sinensis powder, rehmannia powder, eucommia ulmoides leaf powder, fingered citron powder, rhizoma polygonati powder, sea-buckthorn powder, spina date seed powder, coix seed powder, donkey-hide gelatin powder, poria cocos powder, liquorice powder, longan aril powder, Chinese date powder, wolfberry fruit powder, longan powder and Chinese yam powder. By optimizing the components and combining the specific traditional Chinese medicine extract, ergothioneine and nervonic acid, it is found that the three components have the functions of synergistically promoting immunity and protecting skin, and under the specific proportion, the prepared milk powder for women has the better functions of promoting immunity and protecting skin.

The invention discloses a cordyceps militaris polysaccharide fluorescent carbon dot capable of being used for detecting and degrading tetracycline antibiotics as well as a preparation method and application thereof, and belongs to the technical field of nano materials and environmental monitoring. The cordyceps militaris polysaccharide fluorescent carbon dots are prepared by the following steps: dissolving cordyceps militaris polysaccharide and zinc chloride in water, and then carrying out high-temperature reaction; and after the reaction is finished, filtering, dialyzing and drying to obtain the cordyceps militaris polysaccharide fluorescent carbon dots. The carbon quantum dot prepared by the invention realizes the integration of detection and degradation of tetracycline antibiotics, and has a series of advantages of simplicity in operation, high selectivity, low detection limit, environmental friendliness and the like, thereby having important value in the technical fields of nano materials and environmental monitoring.

The invention relates to the field of functional food processing, and discloses a qi-tonifying and blood-activating dietary therapy formula based on the theory of qi and blood in traditional Chinese medicine, which comprises astragalus membranaceus, angelica sinensis, red dates, lycium barbarum, lycium ruthenicum, ginseng, salvia miltiorrhiza, mulberry, lucid ganoderma, cordyceps mycelium, rhodiola rosea, American ginseng, acanthopanax, grape seed extract and chrysanthemum. Through a compound formula based on the theory of qi and blood in traditional Chinese medicine, and by combining various core medicinal materials and functional plants such as the astragalus membranaceus, the angelica sinensis, the ginseng, the lucid ganoderma, the rhodiola rosea and the fructus lycii, the multi-level effects of tonifying qi, enriching blood, activating blood, resisting oxidation and regulating immunity are achieved. The technical effects of comprehensively regulating qi and blood and improving body immunity and resistance are achieved through the synergistic effect of the compound components, compared with a scheme of regulating qi and blood by depending on a single component in the prior art, the compound formula remarkably improves the overall drug effect, solves the problems that a single component is limited in regulating function and insufficient in curative effect, and is suitable for popularization and application. Therefore, the requirements of modern people on comprehensive conditioning and immunity enhancement are met.

The invention provides a cordyceps militaris fermentation extract and application thereof in preservation of agaricus bisporus, and belongs to the technical field of deep processing of edible mushrooms and preservation of agricultural products. The preparation method of the cordyceps militaris fermentation extract comprises the following steps: (1) taking cordyceps militaris sporocarp, adding water, grinding, and performing enzymolysis by using cellulase and papain in sequence to obtain cordyceps militaris pulp; (2) adding glucose and waste beer yeast powder into the cordyceps militaris pulp, then inoculating lactobacillus paracasei and bifidobacterium bifidum, and fermenting; and (3) after fermentation is completed, carrying out ultrasonic crushing, centrifuging to remove thalli, and carrying out spray drying to obtain the cordyceps militaris fermentation extract. The method can effectively reduce the browning index and weight loss ratio of agaricus bisporus, can slow down the increase of malonaldehyde, reduces the activity of PPO enzyme, and prevents enzymatic browning caused by the synergistic effect of the PPO enzyme and peroxidase. The SOD enzyme activity in the agaricus bisporus can be kept, active oxygen in tissues can be removed, and the metabolic balance of the active oxygen can be maintained.

The invention relates to a cordyceps militaris cultivation device which comprises a cultivation frame and a cultivation box, the cultivation frame comprises a plurality of sets of independent cultivation chambers, and each set of cultivation chambers are divided into cultivation cavities of a plurality of levels according to cultivation stages; an inoculated culture medium is contained in the culture box; each group of cultivation chambers is internally provided with an independent transfer mechanism, the transfer mechanisms control the cultivation boxes to gradually transfer from low-grade cultivation cavities to high-grade cultivation cavities according to needs, each cultivation cavity is internally provided with an independent illumination system, and the cultivation cavities of the same grade are internally provided with the same ventilation system; the cultivation boxes in the static state and the corresponding cultivation cavities form closed or sealed spaces; efficient partitioned independent cultivation management is achieved, environment parameters such as illumination, temperature and humidity can be better controlled on the basis of the growth stage of mycelia, high-yield and high-quality cordyceps militaris can be obtained, and the growth processes of the mycelia in different cultivation boxes can be compared and observed more visually.

The invention discloses a preparation method of cordyceps sobolifera polysaccharide and application of the cordyceps sobolifera polysaccharide in relieving acute chemical liver injury. Monosaccharide of the cordyceps sobolifera polysaccharide (abbreviated as CCPW) is composed of glucose, mannose and galactose according to the molar ratio of 8.918: 1: 0.356, and the relative molecular mass is 2.62 * 10 < 4 > Da. Efficacy experiments show that the cordyceps sobolifera homogeneous polysaccharide CCPW can significantly inhibit increase of ALT and AST in a CCl4-induced acute chemical liver injury model mouse, effectively improve the degree of liver cell disruption and swelling, relieve liver inflammatory response, relieve CCl4-induced mouse liver tissue injury, protect mouse intestinal barriers, recover intestinal microbial balance and have obvious liver protection activity. The cordyceps sobolifera polysaccharide CCPW is clear in composition and remarkable in effect, and has wide development and application prospects in the aspect of relieving acute chemical liver injury.

The invention discloses a method for preparing a cordyceps culture medium by taking polygonatum kingianum as a culture medium, and relates to the technical field of culture medium preparation. The method comprises the following steps: performing enzymolysis treatment on a polygonatum kingianum raw material to obtain enzymatic hydrolysate rich in micromolecular nutrition; active components of polygonatum kingianum are extracted to prepare microcapsules with response release characteristics; preparing at least two kinds of 3D biological printing ink by utilizing the enzymatic hydrolysate and a component containing the microcapsule and a polygonatum kingianum material which is not subjected to enzymolysis; the ink is constructed into a three-dimensional culture medium with a layered or gradient structure layer by layer through a 3D printing technology and is solidified, the culture medium prepared by the method can realize gradient distribution of nutrients and programmed release of active ingredients, and an optimized microenvironment is provided for growth of cordyceps sinensis, so that the culture efficiency, yield and quality of cordyceps sinensis are effectively improved.

The invention provides a cordyceps militaris mycelium and a preparation method and application thereof, and relates to the technical field of biological materials. According to the invention, chitinase is added into the cordyceps militaris mycelium material to carry out enzymolysis on chitin to obtain chitooligosaccharide and N-acetylglucosamine, so that beta-glucan in a mycelium framework is dissolved out, and the grafting efficiency of the cordyceps militaris mycelium material is improved; furthermore, the antibacterial effect of the antibacterial material is improved by carrying out grafting reaction on the cordyceps militaris mycelium material and an exogenous monomer antibacterial peptide which has antibacterial performance and contains carboxyl groups.

The invention belongs to the technical field of medicine crystals, and relates to cordycepin-oxalate as well as a preparation method and application thereof. The invention relates to a preparation method of cordycepin-oxalate. The preparation method comprises the following steps: (1) physical mixing: sufficiently and uniformly mixing oxalic acid and cordycepin-crystal form II crystals; (2) liquid auxiliary grinding: adding an auxiliary agent, and continuously grinding to obtain a solid mixture; and (3) drying the solid mixture to obtain the cordycepin-oxalate. According to the method, the cordycepin-oxalic acid eutectic salt is prepared for the first time, the dissolution rate of the cordycepin-oxalic acid eutectic salt is obviously lower than the dissolution rate of cordycepin reported in the prior art, and the cordycepin-oxalic acid eutectic salt has extremely high potential medicinal application value. The cordycepin-oxalic acid eutectic salt has important technical innovation and market application potential due to the slow release effect.

The invention discloses application of a traditional Chinese medicine composition in preparation of a product for resisting colon cancer and tumor hemagglutinin, and relates to the technical field of biology. The traditional Chinese medicine composition is prepared from the following components: 5-150 parts of American ginseng, 5-160 parts of lucid ganoderma, 1-90 parts of fermented cordyceps sinensis powder and 5-90 parts of roses. Or 5-150 parts of American ginseng, 5-160 parts of lucid ganoderma, 1-60 parts of cordyceps sinensis and 5-90 parts of rose. The traditional Chinese medicine composition provided by the invention can improve weight loss related to tumors, inhibit tumor growth, recover food intake, improve the athletic ability of a patient, relieve muscle and fat atrophy, inhibit spleen swelling, improve anemia and peripheral inflammation, and effectively relieve cancer-related fatigue caused by colon cancer, so that the traditional Chinese medicine composition provided by the invention has the advantages that the effect is obvious; the traditional Chinese medicine composition can be used for effectively treating colon cancer and malignant fluid, and has a wide application prospect in preparation of products for treating colon cancer and malignant fluid.

The invention provides a composition for improving a barrier function and application thereof, and relates to the technical field of cell injury nursing. Gamma-aminobutyric acid (GABA) and cordyceps militaris fermentation filtrate (FCM) are used for treating barrier damage for the first time. Specifically, the combination of the gamma-aminobutyric acid and the cordyceps militaris fermentation filtrate can significantly improve damage of an epithelial mechanical barrier and an immune barrier caused by external stimulation, so that the epithelial barrier function can be prevented and improved from multiple dimensions.

The invention relates to the technical field of mycelium culture, and provides a culture method and application of cordyceps militaris mycelium with high selenium content and high cordycepin content. The culture method comprises the following steps: inoculating cordyceps militaris mycelia into a liquid culture medium, and carrying out shake culture for 4-7 days to obtain cordyceps militaris liquid seeds; adding a plant selenium source into the solid culture medium, sterilizing to obtain a cordyceps militaris selenium-rich solid culture medium, adding the cordyceps militaris liquid seeds into the cordyceps militaris selenium-rich solid culture medium, and continuously culturing for 30-40 days to obtain the high-selenium and high-cordycepin cordyceps militaris mycelium. According to the culture method, the steps are simple and convenient, the raw materials are cheap and safe, the total selenium content of the produced mycelium is 0.76-191.45 mg / kg, the organic selenium content is more than 99%, the cordycepin content is 17707.3-24769 mg / kg, the content of various effective components such as the total selenium, the organic selenium and the cordycepin is outstanding, the yield is high, standardized popularization and application of the selenium industry in multiple fields are facilitated, and the wide market prospect is achieved.

This invention discloses a protein-polysaccharide ternary nanoparticle loaded with cordycepin, its preparation method, and its applications. The invention employs a layer-by-layer self-assembly method to modify the surface of zein nanoparticles. Since zein has a positive surface charge, the first layer modification uses the anionic polysaccharide sodium alginate for electrostatic bonding, and the second layer modification uses the cationic polysaccharide chitosan, constructing positively charged ternary composite nanoparticles. These nanoparticles have small particle size, high encapsulation efficiency, high drug loading capacity, and a sustained-release effect, significantly reducing cytotoxicity and significantly improving the therapeutic effect on osteoarthritis. This invention develops a novel cordycepin formulation, overcoming the limitations of cordycepin's clinical use.

The invention provides a peptide beverage capable of dispelling effects of alcohol, protecting liver and relieving fatigue and a preparation method and application thereof.The peptide beverage comprises hovenia dulcis thunb oligopeptide, walnut peptide, antioxidant peptide, energy release auxiliary peptide and the like, and the antioxidant peptide is prepared by mixing cordyceps militaris, sea-buckthorn and sunflower heads. The energy release auxiliary peptide is prepared from carnosine and arginine succinic acid peptide through microbial fermentation. The preparation method comprises the following steps: mixing the components according to a certain ratio, stirring, homogenizing, sterilizing and the like. The peptide beverage has the effects of dispelling the effects of alcohol, protecting the liver and relieving fatigue, and is suitable for preparing drugs or health-care products for dispelling the effects of alcohol and protecting the liver. The preparation method has the characteristics of strict raw material selection and fine preparation process, and ensures the quality and efficacy of the product. Through a scientific ratio and an advanced preparation technology, the peptide beverage provides a new choice for dispelling the effects of alcohol, protecting the liver and relieving fatigue, and has a wide market application prospect.

The invention discloses application of cordyceps javanica Bd01 and a dispersible oil suspending agent thereof to prevention and control of spodoptera frugiperda, and relates to the technical field of biology. The research finds that the cordyceps javanica Bd01 has a strong infection lethal effect on larvae of spodoptera frugiperda at all ages, the pupation rate and eclosion rate of the larvae can be remarkably reduced, and the breeding cycle of pests can be effectively blocked. The invention further develops a dispersible oil suspending agent, and the suspending rate is effectively improved through formula optimization. The dosage form takes soybean oil as a dispersion medium, does not contain harmful solvents such as methylbenzene, is free of dust pollution and is friendly to operators and the environment, and ascorbic acid is added to improve the ultraviolet resistance of spores and prolong the effective period of the pesticide effect. In addition, the preparation also has a certain control effect on caffeinidae, is wide in application scene, provides a new scheme for green control of spodoptera frugiperda, solves the problems of chemical pesticide resistance and residue, and accords with the development requirements of green agriculture.

The invention discloses rapid detection equipment for cordyceps sinensis. The rapid detection equipment comprises an image capturing system, a cordyceps sinensis real-time detection system and an alarm system, the image capturing system consists of a plurality of cameras and is used for acquiring multi-angle image data of an object to be detected; the cordyceps sinensis real-time detection system is composed of an automatic cordyceps sinensis detection model running based on a jetson nano platform and is used for rapidly detecting an object to be detected; according to the automatic detection method of the cordyceps sinensis, an improved YOLOv8 target detection algorithm is used. The method has the advantages that a picker holds the rapid cordyceps sinensis detection equipment by hand and does not need to climb to the ground to damage the local environment, the recognition efficiency can be greatly improved, the required labor cost is reduced, the damage to the ecological environment caused by a traditional multi-manpower and low-efficiency search mode is avoided, and the method is suitable for popularization and application. The supply amount of the cordyceps sinensis can be increased to a certain extent, the income of peasants in Tibetan areas is increased, and then sustainable growth of the cordyceps sinensis in the Qinghai-Tibet Plateau areas is protected.

The invention discloses a cordyceps militaris strain with high yield of ergothioneine and application of the cordyceps militaris strain, and belongs to the technical field of microorganisms and fermentation engineering. On the basis of the screened cordyceps militaris strain with high-yield ergothioneine potential, the cordyceps militaris strain is subjected to ultraviolet mutagenesis treatment, and a cordyceps militaris mutant strain which can be stably inherited and has high-yield ergothioneine is obtained through screening. According to the method, the yield of ergothioneine, cordycepin and cordyceps adenosine in the cordyceps militaris strain is synergistically improved on the basis of precursor directional metabolism remodeling by optimizing and screening precursor substances. The extracting solution of the cordyceps militaris strain can improve the survival rate of lactobacillus reuteri at the high temperature of 50 DEG C and has a protection effect on cells, and the active ingredients of the cordyceps militaris strain can provide an antioxidant protection effect to improve the viability of the cells in an extreme environment.

The invention belongs to the field of health care products and biological medicines, and particularly discloses an anti-aging stem cell PQQ cordyceps sinensis ganoderma lucidum anthocyanin compound preparation and application thereof.The preparation is composed of apple stem cell extract, PQQ, cordyceps militaris extract, ganoderma lucidum extract, procyanidine and pharmaceutic adjuvants and can be made into capsules or pills or tablets. The preparation method comprises the following steps: extracting the raw material components, uniformly mixing, adding the procyanidine and the auxiliary materials, performing high-pressure homogenization treatment, and finally preparing different dosage forms. Wherein the preparation of the apple stem cell extract, the cordyceps militaris extract and the lucid ganoderma extract comprises detailed steps. The compound preparation can be used for preparing health care products or medicines for preventing / treating senile nervous system diseases, especially medicines for preventing / improving Alzheimer's symptoms. In addition, the invention also provides a pharmaceutical composition containing the compound preparation and an acetylcholin esterase inhibitor.

The invention belongs to the technical field of functional food, and particularly relates to a compound fermentation composition for regulating immunity and intestinal microecology as well as a preparation method and application of the compound fermentation composition. The invention provides a compound fermentation composition for regulating immunity and intestinal microecology. Comprising the following components: blueberry freeze-dried powder, an astragalus extract, a lycium ruthenicum extract, a day lily flower extract, grifola frondosa polysaccharide, schizochytrium limacinum powder, a red yeast rice extract, polygonatum polysaccharide, cordyceps militaris, xylooligosaccharide, yeast beta-glucan, lactoferrin, stevioside, a complex microbial inoculant and a complex enzyme. According to the compound fermentation composition disclosed by the invention, high-efficiency immunoregulation, long-acting intestinal balance and maximum component utilization are realized, and an efficient, stable and applicable solution is provided for regulating immunity and intestinal microecology.

The invention mainly relates to a composition for reducing fat and losing weight as well as a preparation method and application thereof, and belongs to the technical field of biology. The composition comprises 30-60 parts of cordyceps flower sporocarp particles, 5-20 parts of morchella sporocarp particles, 10-25 parts of tartary buckwheat powder, 10-25 parts of a mulberry leaf water extract, 5-21 parts of a rhizoma polygonati water extract and 1-6 parts of a cassia seed water extract. The raw materials are mixed to obtain the composition. The composition can improve the blood fat level of hyperlipidemia, inhibit weight increase, reduce the volume of white fat cells, inhibit liver lipid deposition, reverse liver tissue and liver function damage, effectively reduce lipid and lose weight, and play a role in treating hyperlipidemia, obesity and fatty liver.

The present application relates to the field of gene editing technology, bioengineering and application of microbial fermentation, and discloses a Pichia pastoris high-efficiency gene editing system, a construction method thereof and application in a high-yield cordycepin engineering strain.The construction of the Pichia pastoris CRISPR-Cas9 gene editing system mainly includes the following steps: the histidine HIS4 nutritional deficiency back complementation is used to realize the resistance-free assembly of KhCas9; the sgRNA-tRNA array and the IIS type restriction endonuclease combination are used to simplify the gene editing operation and realize the simultaneous and high-efficiency editing of multiple genes; and the Brex27 domain is fused with KhCas9 to strengthen the homologous recombination efficiency. By using the gene editing system, through the optimization of methanol assimilation, 3'-AMP and adenosine precursor supply, ATP / NADPH energy supply and the like, the construction of a cordycepin engineering strain without resistance gene residues is realized for the first time, the gene editing efficiency of Pichia pastoris is greatly improved, and a powerful tool is provided for the industrialized production of cordycepin.

The invention discloses paecilomyces hepialid powder and a production process thereof, and belongs to the technical field of microorganisms. The production process comprises the following steps: S01, slant strain preparation; s02, shaking the seed solution in a flask; s03, fermenting in a fermentation tank; s04, performing centrifugal separation; s05, drying is carried out; and S06, crushing and sieving to obtain the paecilomyces hepialid powder. According to the paecilomyces hepialid powder obtained by the invention, the yield is 42 to 55 g / L, and the adenosine content is 13.29 to 14.83 g / kg; the content of cordycepin is 2.14 to 3.08 g / kg. Static-high-temperature fermentation is introduced in the fermentation process, the obtained paecilomyces hepialid powder has the characteristics of high biomass, high adenosine content and high cordycepin content, the problems of scarcity of cordyceps sinensis resources and high medicine price can be solved, the contradiction between supply and demand can be relieved, the environment can be protected, the cost of the cordyceps sinensis preparation can be reduced, and the application prospect is wide. And the effect of a medication group of the cordyceps sinensis preparation as a tonic drug is increased.

The invention discloses a traditional Chinese medicine composition for treating recurrent urinary tract infection as well as a preparation method and application thereof. The traditional Chinese medicine composition is prepared from 30-90 parts of astragalus membranaceus, 3-9 parts of cordyceps mycelia, 12-36 parts of rhizoma anemarrhenae, 10-30 parts of golden cypress and 6-18 parts of cinnamon. According to the pathogenesis characteristics of recurrent urinary tract infection and the principle of treatment based on syndrome differentiation of traditional Chinese medicine, the optimal raw material composition and dosage part ratio are obtained through screening, simplification and optimization of a large number of experiments, and the traditional Chinese medicine composition can remarkably improve the frequent micturition symptom, bacterial load and bladder pathological injury of rUTI mice. In addition, the drug-containing serum can promote migration of the SV-HUC-1 cells and reduce the level of inflammatory factors secreted by co-culture of the SV-HUC-1 cells and the UTI89 cells; the adhesion and internalization of the UTI89 to the cells are reduced. In addition, according to different bacterial strains and mouse modeling results, it is found that the modeling rate is obviously increased in a mode of enhancing infection every 24 hours, a recurrent urinary tract infection model of UTI89 infected C57BL / 6J is constructed, and a stable model is provided for screening drugs for treating recurrent urinary tract infection.

The invention discloses a composition for resisting altitude stress and improving hypoxia tolerance, and belongs to the field of nutritional health-care food and functional compositions. The composition comprises the following components in parts by mass: 20-60 parts of beet root powder, 10-50 parts of cordyceps militaris powder, 5-40 parts of astragalus membranaceus powder, 0.5-5 parts of ginseng powder and 1-10 parts of Chinese wolfberry fruit powder. The composition can be prepared into foodstuffs such as soft sweets, tablets, powder, drinks, capsules and the like or other nutritional supplement forms, so that the problems of single effect, indefinite action mechanism and poor compliance of the existing product are solved, and multi-target regulation of hematopoiesis enhancement, energy metabolism improvement, oxidative stress inhibition and circulation improvement is realized.