370 results about "Cordyceps sp." patented technology

The invention provides cytokine hydrogel as well as a preparation method and application thereof in skin injury repair, and belongs to the technical field of biological medicines. Comprising the following steps: (1) adding beta-GP into a chitosan solution to obtain a CS-beta-GP pregel solution; (2) dissolving Pluronic F127 and hyaluronic acid in a PBS (Phosphate Buffer Solution) until the Pluronic F127 and the hyaluronic acid are completely transparent to obtain an F127-HA solution; and (3) sequentially dissolving trehalose, cordycepin, a cell factor composition and collagen peptide in a PBS solution, incubating to obtain a cell factor mixed solution, and mixing the cell factor mixed solution with the CS-beta-GP pregel solution and the F127-HA solution. According to the invention, a dual temperature-sensitive system is established, the gel can be quickly formed under the conditions of injection at low temperature and body temperature, the effective period of active ingredients is prolonged by utilizing a trehalose stabilization technology, and meanwhile, the anti-inflammatory effect of cordycepin and the cell repair effect of collagen peptide are combined, so that the efficient repair of skin injury can be realized.

The invention relates to a cordyceps militaris cultivation device which comprises a cultivation frame and a cultivation box, the cultivation frame comprises a plurality of sets of independent cultivation chambers, and each set of cultivation chambers are divided into cultivation cavities of a plurality of levels according to cultivation stages; an inoculated culture medium is contained in the culture box; each group of cultivation chambers is internally provided with an independent transfer mechanism, the transfer mechanisms control the cultivation boxes to gradually transfer from low-grade cultivation cavities to high-grade cultivation cavities according to needs, each cultivation cavity is internally provided with an independent illumination system, and the cultivation cavities of the same grade are internally provided with the same ventilation system; the cultivation boxes in the static state and the corresponding cultivation cavities form closed or sealed spaces; efficient partitioned independent cultivation management is achieved, environment parameters such as illumination, temperature and humidity can be better controlled on the basis of the growth stage of mycelia, high-yield and high-quality cordyceps militaris can be obtained, and the growth processes of the mycelia in different cultivation boxes can be compared and observed more visually.

The invention discloses a method for preparing a cordyceps culture medium by taking polygonatum kingianum as a culture medium, and relates to the technical field of culture medium preparation. The method comprises the following steps: performing enzymolysis treatment on a polygonatum kingianum raw material to obtain enzymatic hydrolysate rich in micromolecular nutrition; active components of polygonatum kingianum are extracted to prepare microcapsules with response release characteristics; preparing at least two kinds of 3D biological printing ink by utilizing the enzymatic hydrolysate and a component containing the microcapsule and a polygonatum kingianum material which is not subjected to enzymolysis; the ink is constructed into a three-dimensional culture medium with a layered or gradient structure layer by layer through a 3D printing technology and is solidified, the culture medium prepared by the method can realize gradient distribution of nutrients and programmed release of active ingredients, and an optimized microenvironment is provided for growth of cordyceps sinensis, so that the culture efficiency, yield and quality of cordyceps sinensis are effectively improved.

The invention belongs to the technical field of medicine crystals, and relates to cordycepin-oxalate as well as a preparation method and application thereof. The invention relates to a preparation method of cordycepin-oxalate. The preparation method comprises the following steps: (1) physical mixing: sufficiently and uniformly mixing oxalic acid and cordycepin-crystal form II crystals; (2) liquid auxiliary grinding: adding an auxiliary agent, and continuously grinding to obtain a solid mixture; and (3) drying the solid mixture to obtain the cordycepin-oxalate. According to the method, the cordycepin-oxalic acid eutectic salt is prepared for the first time, the dissolution rate of the cordycepin-oxalic acid eutectic salt is obviously lower than the dissolution rate of cordycepin reported in the prior art, and the cordycepin-oxalic acid eutectic salt has extremely high potential medicinal application value. The cordycepin-oxalic acid eutectic salt has important technical innovation and market application potential due to the slow release effect.

The invention discloses application of a traditional Chinese medicine composition in preparation of a product for resisting colon cancer and tumor hemagglutinin, and relates to the technical field of biology. The traditional Chinese medicine composition is prepared from the following components: 5-150 parts of American ginseng, 5-160 parts of lucid ganoderma, 1-90 parts of fermented cordyceps sinensis powder and 5-90 parts of roses. Or 5-150 parts of American ginseng, 5-160 parts of lucid ganoderma, 1-60 parts of cordyceps sinensis and 5-90 parts of rose. The traditional Chinese medicine composition provided by the invention can improve weight loss related to tumors, inhibit tumor growth, recover food intake, improve the athletic ability of a patient, relieve muscle and fat atrophy, inhibit spleen swelling, improve anemia and peripheral inflammation, and effectively relieve cancer-related fatigue caused by colon cancer, so that the traditional Chinese medicine composition provided by the invention has the advantages that the effect is obvious; the traditional Chinese medicine composition can be used for effectively treating colon cancer and malignant fluid, and has a wide application prospect in preparation of products for treating colon cancer and malignant fluid.

The invention provides a composition for improving a barrier function and application thereof, and relates to the technical field of cell injury nursing. Gamma-aminobutyric acid (GABA) and cordyceps militaris fermentation filtrate (FCM) are used for treating barrier damage for the first time. Specifically, the combination of the gamma-aminobutyric acid and the cordyceps militaris fermentation filtrate can significantly improve damage of an epithelial mechanical barrier and an immune barrier caused by external stimulation, so that the epithelial barrier function can be prevented and improved from multiple dimensions.

The invention relates to the technical field of mycelium culture, and provides a culture method and application of cordyceps militaris mycelium with high selenium content and high cordycepin content. The culture method comprises the following steps: inoculating cordyceps militaris mycelia into a liquid culture medium, and carrying out shake culture for 4-7 days to obtain cordyceps militaris liquid seeds; adding a plant selenium source into the solid culture medium, sterilizing to obtain a cordyceps militaris selenium-rich solid culture medium, adding the cordyceps militaris liquid seeds into the cordyceps militaris selenium-rich solid culture medium, and continuously culturing for 30-40 days to obtain the high-selenium and high-cordycepin cordyceps militaris mycelium. According to the culture method, the steps are simple and convenient, the raw materials are cheap and safe, the total selenium content of the produced mycelium is 0.76-191.45 mg / kg, the organic selenium content is more than 99%, the cordycepin content is 17707.3-24769 mg / kg, the content of various effective components such as the total selenium, the organic selenium and the cordycepin is outstanding, the yield is high, standardized popularization and application of the selenium industry in multiple fields are facilitated, and the wide market prospect is achieved.

This invention discloses a protein-polysaccharide ternary nanoparticle loaded with cordycepin, its preparation method, and its applications. The invention employs a layer-by-layer self-assembly method to modify the surface of zein nanoparticles. Since zein has a positive surface charge, the first layer modification uses the anionic polysaccharide sodium alginate for electrostatic bonding, and the second layer modification uses the cationic polysaccharide chitosan, constructing positively charged ternary composite nanoparticles. These nanoparticles have small particle size, high encapsulation efficiency, high drug loading capacity, and a sustained-release effect, significantly reducing cytotoxicity and significantly improving the therapeutic effect on osteoarthritis. This invention develops a novel cordycepin formulation, overcoming the limitations of cordycepin's clinical use.

The invention discloses application of cordyceps javanica Bd01 and a dispersible oil suspending agent thereof to prevention and control of spodoptera frugiperda, and relates to the technical field of biology. The research finds that the cordyceps javanica Bd01 has a strong infection lethal effect on larvae of spodoptera frugiperda at all ages, the pupation rate and eclosion rate of the larvae can be remarkably reduced, and the breeding cycle of pests can be effectively blocked. The invention further develops a dispersible oil suspending agent, and the suspending rate is effectively improved through formula optimization. The dosage form takes soybean oil as a dispersion medium, does not contain harmful solvents such as methylbenzene, is free of dust pollution and is friendly to operators and the environment, and ascorbic acid is added to improve the ultraviolet resistance of spores and prolong the effective period of the pesticide effect. In addition, the preparation also has a certain control effect on caffeinidae, is wide in application scene, provides a new scheme for green control of spodoptera frugiperda, solves the problems of chemical pesticide resistance and residue, and accords with the development requirements of green agriculture.

The invention discloses rapid detection equipment for cordyceps sinensis. The rapid detection equipment comprises an image capturing system, a cordyceps sinensis real-time detection system and an alarm system, the image capturing system consists of a plurality of cameras and is used for acquiring multi-angle image data of an object to be detected; the cordyceps sinensis real-time detection system is composed of an automatic cordyceps sinensis detection model running based on a jetson nano platform and is used for rapidly detecting an object to be detected; according to the automatic detection method of the cordyceps sinensis, an improved YOLOv8 target detection algorithm is used. The method has the advantages that a picker holds the rapid cordyceps sinensis detection equipment by hand and does not need to climb to the ground to damage the local environment, the recognition efficiency can be greatly improved, the required labor cost is reduced, the damage to the ecological environment caused by a traditional multi-manpower and low-efficiency search mode is avoided, and the method is suitable for popularization and application. The supply amount of the cordyceps sinensis can be increased to a certain extent, the income of peasants in Tibetan areas is increased, and then sustainable growth of the cordyceps sinensis in the Qinghai-Tibet Plateau areas is protected.

The invention discloses a cordyceps militaris strain with high yield of ergothioneine and application of the cordyceps militaris strain, and belongs to the technical field of microorganisms and fermentation engineering. On the basis of the screened cordyceps militaris strain with high-yield ergothioneine potential, the cordyceps militaris strain is subjected to ultraviolet mutagenesis treatment, and a cordyceps militaris mutant strain which can be stably inherited and has high-yield ergothioneine is obtained through screening. According to the method, the yield of ergothioneine, cordycepin and cordyceps adenosine in the cordyceps militaris strain is synergistically improved on the basis of precursor directional metabolism remodeling by optimizing and screening precursor substances. The extracting solution of the cordyceps militaris strain can improve the survival rate of lactobacillus reuteri at the high temperature of 50 DEG C and has a protection effect on cells, and the active ingredients of the cordyceps militaris strain can provide an antioxidant protection effect to improve the viability of the cells in an extreme environment.

The invention belongs to the technical field of functional food, and particularly relates to a compound fermentation composition for regulating immunity and intestinal microecology as well as a preparation method and application of the compound fermentation composition. The invention provides a compound fermentation composition for regulating immunity and intestinal microecology. Comprising the following components: blueberry freeze-dried powder, an astragalus extract, a lycium ruthenicum extract, a day lily flower extract, grifola frondosa polysaccharide, schizochytrium limacinum powder, a red yeast rice extract, polygonatum polysaccharide, cordyceps militaris, xylooligosaccharide, yeast beta-glucan, lactoferrin, stevioside, a complex microbial inoculant and a complex enzyme. According to the compound fermentation composition disclosed by the invention, high-efficiency immunoregulation, long-acting intestinal balance and maximum component utilization are realized, and an efficient, stable and applicable solution is provided for regulating immunity and intestinal microecology.

The present application relates to the field of gene editing technology, bioengineering and application of microbial fermentation, and discloses a Pichia pastoris high-efficiency gene editing system, a construction method thereof and application in a high-yield cordycepin engineering strain.The construction of the Pichia pastoris CRISPR-Cas9 gene editing system mainly includes the following steps: the histidine HIS4 nutritional deficiency back complementation is used to realize the resistance-free assembly of KhCas9; the sgRNA-tRNA array and the IIS type restriction endonuclease combination are used to simplify the gene editing operation and realize the simultaneous and high-efficiency editing of multiple genes; and the Brex27 domain is fused with KhCas9 to strengthen the homologous recombination efficiency. By using the gene editing system, through the optimization of methanol assimilation, 3'-AMP and adenosine precursor supply, ATP / NADPH energy supply and the like, the construction of a cordycepin engineering strain without resistance gene residues is realized for the first time, the gene editing efficiency of Pichia pastoris is greatly improved, and a powerful tool is provided for the industrialized production of cordycepin.

The invention discloses paecilomyces hepialid powder and a production process thereof, and belongs to the technical field of microorganisms. The production process comprises the following steps: S01, slant strain preparation; s02, shaking the seed solution in a flask; s03, fermenting in a fermentation tank; s04, performing centrifugal separation; s05, drying is carried out; and S06, crushing and sieving to obtain the paecilomyces hepialid powder. According to the paecilomyces hepialid powder obtained by the invention, the yield is 42 to 55 g / L, and the adenosine content is 13.29 to 14.83 g / kg; the content of cordycepin is 2.14 to 3.08 g / kg. Static-high-temperature fermentation is introduced in the fermentation process, the obtained paecilomyces hepialid powder has the characteristics of high biomass, high adenosine content and high cordycepin content, the problems of scarcity of cordyceps sinensis resources and high medicine price can be solved, the contradiction between supply and demand can be relieved, the environment can be protected, the cost of the cordyceps sinensis preparation can be reduced, and the application prospect is wide. And the effect of a medication group of the cordyceps sinensis preparation as a tonic drug is increased.

The invention discloses a traditional Chinese medicine composition for treating recurrent urinary tract infection as well as a preparation method and application thereof. The traditional Chinese medicine composition is prepared from 30-90 parts of astragalus membranaceus, 3-9 parts of cordyceps mycelia, 12-36 parts of rhizoma anemarrhenae, 10-30 parts of golden cypress and 6-18 parts of cinnamon. According to the pathogenesis characteristics of recurrent urinary tract infection and the principle of treatment based on syndrome differentiation of traditional Chinese medicine, the optimal raw material composition and dosage part ratio are obtained through screening, simplification and optimization of a large number of experiments, and the traditional Chinese medicine composition can remarkably improve the frequent micturition symptom, bacterial load and bladder pathological injury of rUTI mice. In addition, the drug-containing serum can promote migration of the SV-HUC-1 cells and reduce the level of inflammatory factors secreted by co-culture of the SV-HUC-1 cells and the UTI89 cells; the adhesion and internalization of the UTI89 to the cells are reduced. In addition, according to different bacterial strains and mouse modeling results, it is found that the modeling rate is obviously increased in a mode of enhancing infection every 24 hours, a recurrent urinary tract infection model of UTI89 infected C57BL / 6J is constructed, and a stable model is provided for screening drugs for treating recurrent urinary tract infection.

The invention discloses a composition for resisting altitude stress and improving hypoxia tolerance, and belongs to the field of nutritional health-care food and functional compositions. The composition comprises the following components in parts by mass: 20-60 parts of beet root powder, 10-50 parts of cordyceps militaris powder, 5-40 parts of astragalus membranaceus powder, 0.5-5 parts of ginseng powder and 1-10 parts of Chinese wolfberry fruit powder. The composition can be prepared into foodstuffs such as soft sweets, tablets, powder, drinks, capsules and the like or other nutritional supplement forms, so that the problems of single effect, indefinite action mechanism and poor compliance of the existing product are solved, and multi-target regulation of hematopoiesis enhancement, energy metabolism improvement, oxidative stress inhibition and circulation improvement is realized.

This invention discloses a highly active compound health supplement made from Cordyceps sinensis, Artemisia argyi, and rhubarb, and its preparation method, belonging to the field of health food and pharmaceutical preparation processing technology. The method includes: adding artificial Cordyceps sinensis ultrafine powder, prepared Rhubarb tanguticum fine powder, Artemisia argyi leaf extract, microcrystalline cellulose PH102, and maltodextrin into a mixer and premixing at 25-35 rpm to allow the powder to adhere to the surface of the excipients; after tableting, the tablets are subjected to ultra-high pressure treatment at 350-380 MPa. This invention achieves uniform distribution and performance optimization of the formulation components without damaging the activity of heat-sensitive components through precise coupling of premixing strength and ultra-high pressure modification parameters. Experiments show that the obtained formulation has an adenosine retention rate ≥96%, a dissolution rate ≥85% after 5 minutes, and excellent moisture resistance and mechanical strength, solving the technical problems of large activity loss, easy moisture absorption, and slow dissolution in traditional formulations.

The invention relates to a lycium barbarum fermented product with a liver protection function as well as a preparation method and application thereof, and belongs to the technical field of fermentation. The fermentation liquor is prepared by taking a specific traditional Chinese medicine compound consisting of radix puerariae, semen hoveniae, fructus lycii, rhizoma polygonati, flos chrysanthemi, pericarpium citri reticulatae and cordyceps militaris as a matrix through a double-bacterium synergistic fermentation process. The preparation method comprises the following steps: firstly, pre-fermenting a compound extract by using Akkermansia muciniphila bacteria, enriching propionic acid and optimizing the matrix; the preparation method comprises the following steps: carrying out primary fermentation by using lactic acid bacteria combined with Lactobacillus paracasei E10, L. paracasei JN-1 and L. paracasei JN-8 (in a volume ratio of 3: 3: 2), and carrying out secondary fermentation by using lactobacillus combined with Lactobacillus paracasei E10, L. paracasei JN-1 and L. paracasei JN-8 (in a volume ratio of 3: 3: 2). The traditional Chinese medicine composition realizes double synergy of efficient conversion of traditional Chinese medicine active ingredients and intestinal-hepatic axis regulation and control, and is suitable for prevention and adjuvant therapy of alcoholic liver injury and non-alcoholic fatty liver disease.

To provide an endoplasmic reticulum stress suppressing composition and a liver function improving composition.SOLUTION: A composition for suppressing endoplasmic reticulum stress and / or a composition for improving hepatic functions, comprising one or more plants selected from the group consisting of Plantago asiatica, Hamilton's spindle tree, panicled hydrangea, Japanese plum yew, Japanese mallotus, and Papaver rhoeas, and / or an extract thereof.SELECTED DRAWING: None

The invention provides a pulmonary nodule targeting food and medicine homologous composition based on metabonomics screening and application thereof. By analyzing a pulmonary nodule specific metabolic profile, active components, namely platycodin D, mogroside V, oyster active peptide and fermented cordycepin, which can cooperatively regulate and control an arachidonic acid inflammation pathway, a glutathione antioxidant pathway and a tricarboxylic acid cycle energy pathway are reversely screened out. Animal experiments prove that the composition can reduce the volume of pulmonary nodules by 58.3%, human body application shows that the nodules of 76.7% of subjects are reduced, and liver and kidney toxicity is avoided. The composition can be prepared into tablets, capsules and other dosage forms, can be used as functional food or adjuvant therapy drugs for high risk groups of pulmonary nodules, and realizes accurate nutrition intervention.

1. Name of the product for this design: Packaging Box (Snow Lotus Cordyceps Mixture). 2. Application of this design: This design is intended for use in pharmaceutical packaging. 3. The key design features of this product are the combination of shape and pattern. 4. The image or photograph that best illustrates the design's key points: a 3D model.

This invention discloses a method and product for preparing Pien Tze Huang herbal wine fermented on Cordyceps militaris culture medium, belonging to the field of health wine brewing technology. This method uses Cordyceps militaris culture medium (SCM) and Pien Tze Huang herbal wine (GFE) as raw materials, and prepares a health wine with hypoglycemic and antioxidant effects through a complex fermentation process including raw material pretreatment, enzymatic hydrolysis, yeast activation, anaerobic fermentation, and post-treatment. It aims to solve the problems of low raw material utilization, low active ingredient content, and insufficient antioxidant activity in existing technologies. Pretreatment employs ultrasonic extraction. Fermentation conditions are anaerobic fermentation at 28℃ for 18 days, with 3% sugar supplementation every 2 days, controlling the alcohol content at 6%-10% vol, and preferably using an SCM:GFE ratio of 2:1. The resulting product has an alcohol content of 8% vol, is rich in total phenols and total flavonoids, has an α-glucosidase inhibition rate of 82.6%, a DPPH free radical scavenging rate of 78.3%, and a FRAP value of 82.6 mM Fe. 2+ Equivalents / mL, with a harmonious flavor and significant health benefits, provide an effective way for the comprehensive development of Cordyceps militaris and Pien Tze Huang.

The application discloses a flowerpot filling system based on industrialized production of Cordyceps flower, and relates to the technical field of mushroom cultivation.The flowerpot filling system based on industrialized production of Cordyceps flower comprises an equal-amount discharging and laying mechanism and a compaction and leveling mechanism, the compaction and leveling mechanism comprises a placing cavity, a planting flowerpot is placed in the placing cavity, and the equal-amount discharging and laying mechanism is arranged on one side of the compaction and leveling mechanism.Through the arrangement of the compaction and leveling mechanism, the compacting treatment is performed on the laid culture medium raw materials, the compacting work of the culture medium can be quickly and uniformly completed, the production efficiency is higher compared with artificial compacting treatment, and the mechanical compacting can ensure that the culture medium in each flowerpot reaches the same compactness, helps to ensure the consistency of product quality, the accurate control of the compacting work can reduce the waste of the culture medium raw materials, realizes the reasonable utilization of resources, and reduces the cost.

The invention discloses a wild breeding optimization method for cordyceps sinensis, and relates to the technical field of cordyceps sinensis breeding. The cordyceps sinensis wild breeding optimization method comprises the following specific steps: separating high-activity strains from wild cordyceps sinensis, optimizing a synthesis path of key components by combining the screened high-activity strains with a gene editing technology, and inoculating pupae with the finally obtained bacterial liquid. And placing in a culture column to optimize the growth speed of hyphae, completing optimization of the content of active components, and simulating plateau low-temperature and low-oxygen environment in which the culture column is located. A metabolic pathway is optimized by combining high-activity strain screening and gene editing, the synthesis efficiency of key active components such as cordycepin and adenosine is remarkably improved, an ecological column with the light transmittance being larger than or equal to 85% is constructed, key ecological parameters of the high altitude of the Qinghai-Tibet Plateau are simulated and reproduced by combining ultraviolet intensity regulation and control and a low-temperature and low-oxygen environment, and the application prospect is wide. The breeding problem of loss of active ingredients in low-altitude cultivation is solved.

The invention discloses an immunomodulatory composition based on a cordycepin-selenium nano chelate and a preparation method thereof, and belongs to the technical field of biological medicines.The immunomodulatory composition is characterized in that 15-25 nm zero-valent selenium nano particles are chelated through hydroxyl of cordycepin and N7 site bidentate coordination, and a stable five-membered ring structure with the molar ratio is formed; the preparation method comprises the following steps: reducing sodium selenite in an inert atmosphere to generate selenium colloid, chelating the selenium colloid with a cordycepin ethanol solution, and carrying out ultrafiltration and freeze-drying to obtain the product. The invention aims to solve the problems of fuzzy structure, inconsistent batch, uncontrollable immunomodulatory function and the like caused by non-specific mixing in the prior art, and the composition can bidirectionally regulate and control IRF3 pathways, promote polarization of M2 type macrophages and proliferation of Treg cells, and has a clear immunomodulatory mechanism and excellent stability.

The invention discloses an application of a cordyceps militaris germ extract in preparation of an anti-tobacco mosaic virus pesticide, and belongs to the technical field of pesticides. The cordyceps militaris embryo extract has the effect of inhibiting activity on TMV, provides a new way for efficient utilization of cordyceps militaris embryos, promotes high-quality development of the cordyceps militaris cultivation industry, and has important production value and practical significance.

The invention provides a sensor-based cordyceps militaris fermented beverage fermentation evaluation method and system, and relates to the technical field of fermentation engineering.Multi-frequency ultrasonic signals are transmitted and received through an ultrasonic transceiver array deployed on the outer wall of a fermentation tank; and reconstructing the signal into an acoustic parameter three-dimensional distribution image of the fermentation liquid in the fermentation tank by utilizing a tomography algorithm. And based on a pre-established mapping relation, mapping the acoustic parameter three-dimensional distribution image into three-dimensional distribution of the fermentation state parameters. And finally, based on the three-dimensional distribution of the fermentation state parameters, evaluating the overall real state of the internal environment of the fermentation tank to obtain spatial non-uniformity quantitative data of the internal environment of the fermentation tank, and generating an instruction for regulating and controlling the fermentation process according to the data. The method effectively solves the problem that monitoring of a traditional local sensor is not accurate due to non-uniformity of the internal environment of a large industrial fermentation tank in the prior art.

The invention provides a composition for building bodies and improving sexual functions as well as a preparation method and application of the composition, and relates to the technical field of medicinal and edible traditional Chinese medicines and food resources. The composition is prepared from the following components in parts by mass: 20 to 50 parts of oyster peptide, 5 to 20 parts of maca extract, 4 to 18 parts of fructus lycii extract, 4 to 18 parts of herba cistanche extract, 1.5 to 9 parts of radix ginseng extract, 0.3 to 2 parts of cordyceps militaris extract, 0.3 to 2.5 parts of rhizoma polygonati extract and 0.6 to 1.5 parts of vitamin C. Under the synergistic effect of all the components in the composition formula, the antioxidant activity can be remarkably improved, the effects of resisting fatigue and strengthening the physique are achieved, then the composition is beneficial to the male sexual function in multiple ways, cavernosum smooth muscle relaxation and penile erection are promoted, the effects of building the body and improving the sexual function are achieved, and the composition especially plays an important role in improving the sexual function of middle-aged and elderly people.

The invention provides a recombinant cordyceps militaris strain with high yield of beta-glucan as well as a construction method and application of the recombinant cordyceps militaris strain, and belongs to the technical field of bioengineering and genetic breeding of edible fungi. According to the invention, a cordyceps militaris CmGls super-safe overexpression vector is constructed through a CRISPR-Cas9 technology, and then the cordyceps militaris CmGls super-safe overexpression vector is transferred into cordyceps militaris to obtain a recombinant cordyceps militaris strain with high yield of beta-glucan. According to the recombinant cordyceps militaris strain, membrane binding type beta-1, 3-glucan synthetase gene CmGls is accurately inserted into a safe port site CmSh1 of a cordyceps militaris genome, compared with a wild type cordyceps militaris strain, the growth rate of the recombinant cordyceps militaris strain is remarkably increased by 15% or above, and yellow phenotype and cell wall stress resistance are remarkably enhanced; after 6 days of submerged fermentation, the mycelium amount and the extracellular beta-glucan yield are respectively improved by more than 10%, and the strain has good application in improving beta-glucan synthesis.

The invention provides a preparation method of a seed-dressing agent for preventing rice insect pests and a product thereof. According to the method, the survival rate of cordyceps javanica during long-term storage of the seed dressing agent is improved through gelation of exosporin, addition of pyroclastic tuff and addition of casein; the thallus loading capacity of the seed dressing agent cordyceps javanica is improved through alkali treatment, negative pressure treatment, pressurized circulating filtration loading and lactose addition; by adding the binding agent prepared from the corn husks and adding the exowall essence gel prepared from the pollen, the falling rate of the seed dressing agent is reduced; the thallus slow-release capability of the seed dressing agent is improved by adding the exowall element gel prepared from the pollen; the prevention effect on rice planthoppers is improved through the insect manure, and insect pests are prevented.