1180 results about "Cordyceps sp." patented technology

The invention provides a method of producing worm grass lotus seeds by virtue of solid state fermentation of lotus seeds. The method comprises the following steps: on the basis of utilizing the steamed lotus seeds as a worm grass solid state fermentation culture medium, inoculating 3-8ml worm grass liquid strain to per 100 g of the lotus seeds; culturing for 10-30 days at the temperature of 5-30 DEG C and the humidity of 60-90% in dark, namely obtaining worm grass lotus seeds on which hyphae are overgrown; and culturing the worm grass lotus seeds on which the hyphae are overgrown at the temperature of 5-30 DEG C and the humidity of 80-95% for 10-30 days with the illumination of 8-15 hours per day, thus obtaining the worm grass lotus seeds on which worm grass sporocarps are grown. The invention provides a new worm grass source and a new lotus seed health food. According to the invention, the medical effect of worm grass and the nutrition of the lotus seeds are organically combined, so that the product has various nutrient components, is especially rich in active components such as cordycepin, a cordycepic acid, a worm grass polyose and the like and has high edible and medical values; and the worm grass lotus seed fungus preparation process is simple and can easily realize large-scale production, popularization and implementation.

The invention discloses a health-care product for recuperating specific intrinsic constitution. The health-care product comprises the following raw materials in parts by weight: 1-8 parts of ginseng, 0.5-8 parts of cordyceps militaris, 1-9 parts of rhizoma polygonati and 1-8 parts of lilies; or 1-4 parts of longan aril, 1-5 parts of lotus seed, 1-2 parts of fingered citron, 1-2 parts of Chinese flowering quince, 1-6 parts of angelica dahurica, 1-6 parts of honeysuckle, 1-5 parts of red jujubes, 1-2 parts of mulberry leaves, 2-4 parts of Chinese yam, 2-6 parts of Chinese wolfberry, 1-4 parts of raspberry, 1-4 parts of euglenophyta, 1-2 parts of walnut kernels, 2-6 parts of horseradish tree leaves, 2-6 parts of snow lotus culture, 1-6 parts of male flowers of eucommia ulmoides, 1-8 parts of Peruvian ginseng powder, 1-4 parts of Acai, 1-4 parts of Noni puree, 1-4 parts of eucommia ulmoides seed oil, 1-2 parts of colla corii asini, 1-2 parts of hyacinth bean and 1-4 parts of Chia seed are added. According to the formation mechanism of the specific intrinsic constitution and the recuperating principle of symptoms, (artificially planted) ginseng capable of reinforcing vital energy and improving immunity, cordyceps militaris capable of complementing essence, qi and spirit of a human body, rhizoma polygonati capable of nourishing qi and yin, and the lilies capable of nourishing yin, clearing heat and promoting salivation are carefully selected from new food raw materials to be used as main raw materials, and the advantages of new resources are fully exerted.

The invention provides an extraction and purification method of cordycepin from Cordyceps militaris, and belongs to the deep-processing technical field of agricultural products. The method comprises the following steps: crushing Cordyceps militaris raw material, mixing the raw material with water at 1:20 raw material/water ratio, performing ultrasonic extraction, and performing centrifugal filtration on obtained extracting solution and pouring in a macroporous resin column at the adsorption flow of 1.8BV/hr; taking 15% methanol aqueous solution as an eluant to perform resin column elution, and decompressing and condensing obtained eluent at the temperature of 60 DEG C by a rotary evaporator to obtain Cordyceps militaris cordycepin concentrate. The method can help realize aqueous solution extraction of the cordycepin from the Cordyceps militaris raw material at low temperature, and the extraction rate reaches 85%; and polysaccharide and protein can be removed from the extract by macroporous resin separation and purification, thus the content of the cordycepin in the extract can reach 10%. The cordycepin in the Cordyceps militaris product has high activity, can be taken as a raw material for producing cordycepin monomer and can also be directly taken as an additive of health products and functional food, thus the cordycepin has wide market development prospect.

The invention relates to a Chinese caterpillar fungus instant tea which comprises the raw materials in parts by weight: 20-40 parts of cordyceps fruiting body, 5-15 parts of sealwort, 10-20 parts of medlar, 10-20 parts of puerarin, 10-20 parts of poria cocos, 5-10 parts of lotus seeds, 5-10 parts of ginseng or American ginseng and 40-50 parts of isomalto-oligosaccharide. The Chinese caterpillar fungus instant tea is prepared by carrying out water extraction or superfine grinding on the raw materials, mixing and pelleting. The Chinese caterpillar fungus instant tea is a solid instant drink which has the functions of improving the immunity, protecting the cells of liver and kidney, regulating the intestinal flora, promoting the proliferation of bifidobacterium, improving gastroenteric function and the like for the middle-aged and elderly people and the organism with sub-health state.

The invention relates to a method for extracting a composition containing nano-oligopeptide from cordyceps militaris as well as the composition extracted by the method. In the method, the cordyceps militaris is hydrolyzed by a complex enzymatic method to obtain the small molecular nano-oligopeptide. The invention is characterized in that the molecular weight of the nano-oligopeptide is mainly distributed within 100-1000D; and preferably the Gaussian distribution peak area percentage of the nano-oligopeptide the molecular weight of which is 100-500 D in the composition is more than 50%. The cordyceps militaris oligopeptide extract provided by the invention reserves other active ingredients such as cordycepin, ecdysone, adenosine, cordyceps polysaccharide, cordycepic acid, trace elements, vitamins and free amino acid. The invention further relates to an application of the composition containing the nano-oligopeptide extracted from the cordyceps militaris in preparing medicines, healthcare products and cosmetics.

The invention discloses a culture medium for cultivating cordyceps militaris, a preparation method of the culture medium, a culture method of cordyceps militaris and a preparation method of an oral cordyceps militaris tablet. The culture medium for cultivating cordyceps militaris is an aqueous solution and comprises 130 to 140g/L of dry silkworm chrysalis meal, 60 to 65g/L of rice flour, 1.2 to 1.5g/L of potassium dihydrogen phosphate and 0.8 to 1.2g/L of sodium dihydrogen phosphate. The culture method of cordyceps militaris comprises the following steps of inoculation, culture and harvest re-culture. The preparation method of the oral cordyceps militaris tablet comprises the following steps of drying, multiple cold sterilization treatment, crushing, wet granulation and tabletting. Cordyceps militaris cultured by the culture medium provided by the invention has nutrient content far higher than nutrient content of cordyceps militaris cultured by other culture methods, has a golden color, a luster and a heavy fragrance. In the preparation of the dried oral cordyceps militaris tablet, multiple cold sterilization treatment is carried out after drying. Through low-temperature superfine crushing, a cordyceps militaris spore wall breaking effect is guaranteed and a nutrition constituent loss is reduced.

The present invention discloses a kidney supplementing and fatigue preventing maca compound tableting candy and a preparation method thereof. The kidney supplementing and fatigue preventing maca compound tableting candy is prepared from main materials and accessory materials. The main materials are maca powder, cordyceps militaris powder and ginseng extract. The accessory materials are sorbitol, xylooligosaccharide, maltodextrin and microcrystalline cellulose. The kidney supplementing and fatigue preventing maca compound tableting candy consists of the following raw and accessory materials in weight percentages: 5-20% of maca powder, 1-5% of cordyceps militaris powder, 0.5% of ginseng extract, 20-70% of sorbitol, 2% of xylooligosaccharide, 3% of maltodextrin and 20% of microcrystalline cellulose. The preparation method consists of the following main steps: 1. crushing; 2. mixing; 3. granulating; 4. tableting; and 5. packaging. The maca and cordyceps militaris powder are blended together. The maca contains macaenes and macamides which can promote sexual function. The cordyceps militaris powder can supplement man's essence, qi and mind, is a true energy supplement agent, strengthens the roles of functional nutrients, and has health-care functions of supplementing kidneys and strengthening yang, preventing fatigue and improving sub-health.

The invention relates to health tea with effects of decreasing uric acid and protecting the liver and the kidney and a preparation method thereof. The health tea is prepared from the following raw materials in parts by weight: 20 to 30 parts of kudzuvine roots, 20 to 28 parts of coix seeds, 20 to 25 parts of herba lophatheri, 15 to 25 parts of Poria cocos, 10 to 15 parts of radix glycyrrhizae, 10 to 15 parts of fresh rhizoma imperatae, 8 to 15 parts of folium mori, 5 to 10 parts of fructus lycii, 5 to 10 parts of cordyceps militaris and 1 to 2 parts of enzyme powder. The health tea has the effects of decreasing uric acid and protecting the liver and the kidney, and the functions of clearing away heat and toxic materials, decreasing uric acid and the like are achieved through the effective ingredients; the ingredients such as the herba lophatheri and Poria cocos contained in the health tea are relatively high in diuretic effects, and are absorbed by intestines and stomach when tea water enters the human body, so that the effect of neutralizing alkaline substances in blood is achieved, the alkaline substances are discharged metabolically in a urine form, urate dirty in the kidney can be cleaned, and the capacity of the kidney for filtering out uric acid is increased.

The invention discloses an extraction technology of cordyceps militaris polysaccharide and polypeptide. The extraction technology comprises the following steps: drying cordyceps militaris, grinding cordyceps militaris to a size of 80 to 120 meshes, adding distilled water, evenly stirring, carrying out a supersonic treatment for 1 to 3 minutes; heating to a temperature of 60 to 90 DEG C, carrying out reactions for 20 to 60 minutes under stirring, filtering, collecting the filter cakes and the filtrate, adding ethanol into the filtrate, allowing the filtrate to stand still to precipitate cordyceps militaris polysaccharide; adding the filter cakes into distilled water, stirring, adjusting the pH value of the solution to 8-10 by sodium hydroxide, adding alkaline protease to carry out hydrolysis for 8 to 12 hours, boiling the solution, stopping the enzyme hydrolysis reactions, filtering, and subjecting the filtrate to HPLC purification so as to obtain the cordyceps militaris polypeptide. The provided extraction technology can extract cordyceps militaris polysaccharide and polypeptide at the same time, fully utilizes the cordyceps militaris resources, and moreover has the advantages of mild extraction conditions, high product bioactivity, and simple and easy extraction method.

The invention relates to a lepidium meyenii walp and cordyceps militaris health-care food and a preparation method thereof. The lepidium meyenii walp and cordyceps militaris health-care food comprises the following raw material components in percentage by weight: 30%-40% of lepidium meyenii walp extractives, 10%-20% of cordyceps militaris extractives and 40%-60% of auxiliary materials and is prepared into a tablet and the like through a specific method. The lepidium meyenii walp and cordyceps militaris health-care food disclosed by the invention increases the content of effective components and nutritional components which are contained in the lepidium meyenii walp and the cordyceps militaris, ensures the edible value and health-care value of a lepidium meyenii walp and cordyceps militaris tablet, is simple in preparation method and lower in production cost, achieves the breakthrough development on the aspect of bioavailability of the lepidium meyenii walp and cordyceps militaris tablet and achieves the optimal effect on the aspect of enhancing immunity and especially on the aspect of treating a chronic fatigue syndrome and an asthma syndrome.

The invention discloses a fungal fibrinolytic enzyme and a preparation method thereof. The fungal fibrinolytic enzyme is prepared by taking a Cordyceps militaris strain as a strain and performing liquid fermentation culture, separation and purification. The relative molecular weight of the fibrinolytic enzyme is about 21,000. The Cordyceps militaris fibrinolytic enzyme has good thrombolytic performance, is free from obvious acute toxicity, has prospects for clinical trial, development and application, and adds a new member to a rare fibrinolytic enzyme family. Particularly, a preferable fibrinolytic-enzyme Cordyceps militaris strain C.LSG-1 obtained through severe screening is rough in growth conditions, short in enzyme production cycle and capable of harvesting a large amount of Cordyceps militaris mycelium during enzyme production, and is the strain excellent in production performance. The Cordyceps militaris fibrinolytic enzyme is an extracellular enzyme which is particularly beneficial to subsequent separation and purification during preparation. The fungal fibrinolytic enzyme takes corn protein with extensive sources as a main culture medium, thereby having low cost for raw materials and providing a novel way for developing and utilizing the prior resources.

The invention discloses a passivating agent for reducing the activity of Pb and Cr in alkaline soil and application thereof, and belongs to the field of heavy metal soil remediation. The passivating agent is prepared from, by weight, 5-20 parts of a microorganism bacterium agent, 10-30 parts of pomegranate pomace, 15-35 parts of coffee grounds, 5-30 parts of cordyceps militaris culture medium residues and 10-35 parts of blackberry pomace. Compared with the prior art, the passivating agent can significantly treat the alkaline farmland soil which is slightly polluted by plumbum and chromium, is low in cost and easy to operate and manage and has the very good application and popularization value.

A cordyceps matrix for food is prepared from the high-grade rice, potato and carrot through boiling, high-pressure sterilizing, loading in bottle, preparing nutritive liquid, mixing it with the cordyceps strain liquid, inoculating in into said bottle, and fermenting culture. A cordyceps food is prepared from said cordyceps matrix, cooked rice flour, milk plant ol, cane sugar and additive.

The invention provides a method for preparing polypeptide of Cordyceps militaris by enzymolysis and product development. Cultivated Cordyceps militaris is dried at a temperature of between 37 and 72 DEG C and is crushed into powder, a biocatalyst (enzyme) of which weight is 0.007 to 0.05 percent of the weight of the Cordyceps militaris is added, and a zymoexciter is added at the same time. The hydrolysis is performed for 3 to 6h under water bath condition that the temperature is between 25 and 55 DEG C and the pH value is between 1.0 and 10.0, then the centrifugation and ultrafiltration are performed to obtain a clear yellowy Cordyceps militaris hydrolysate, and the hydrolysis rate is between 70 and 80 percent. The Cordyceps militaris hydrolysate contains a large amount of small molecular polypeptide, growth factors, esters (phospholipid, steroid compounds, fatty acid, and so on), more than 20 amino acids, trace elements and various effective medicinal components such as cordycepin, cordycepic acid, and so on. An obtained product can be applied to a plurality of aspects such as pharmacy, health care products and food, cosmetics, and so on, has convenient application, time saving and no pollution, and can develop a plurality of new products.

The invention relates to a traditional Chinese medicine composition playing roles of stopping bleeding, diminishing inflammations and preventing bacteria and a toothpaste containing the traditional Chinese medicine composition. The traditional Chinese medicine composition comprises the following components: a cordyceps militaris extract, a pseudo-ginseng extract, a scutellaria baicalensis extract and tea polyphenol. The composition provided by the invention plays roles in stopping bleeding, diminishing inflammations and preventing bacteria, and can be applied to the toothpaste to prevent oral diseases.

The invention provides a cordyceps militaris factory production method and process. The cordyceps militaris factory production method and process include the steps of introducing mother seeds and enabling the mother seeds to be separated out, carrying out sporocarp generating tests, expanding propagation of the mother seeds, manufacturing breeder seeds, manufacturing liquid cultivars and making culture media, sterilizing and cooling, inoculating, carrying out production management, carrying out spawn fermentation, cultivating by light, ventilating, carrying out miscellaneous bacterium and pest control, physical control and chemical control, harvesting, managing after the harvest, packaging, transporting and storing. According to the method and process, the yield of cordyceps militaris is high, the operation is easy, the efficiency is high, large-scale production is facilitated, and the produced cordyceps militaris is economical and practical, low in production cost and easy to store and transport.

The invention discloses a cordyceps militaris extraction method and a cigarette containing the cordyceps militaris extract, which aim to solve the technical problems of increasing the cigarette aroma and reducing the objectionable constituents of the cigarette. The cordyceps militaris extraction method comprises the following steps of: drying, crushing, digesting, filtering and concentrating the cordyceps militaris, adding ethanol, standing, concentrating, and obtaining the cordyceps militaris extract. In the cigarette containing the cordyceps militaris extract, a tobacco shred contains the cordyceps militaris extract, and the weight of the cordyceps militaris extract is 0.001-10 percent of that of the tobacco shred. Compared with the prior art, the cordyceps militaris extract which is obtained after extraction is added in the tobacco shred, so the cigarette aroma can be increased, the smoke impact is improved, the smoke dryness of the cigarette is reduced, the smoke taste is improved, and the objectionable constituents of the cigarette are reduced.

The invention relates to a control method of the fingerprint spectrum quality of cordyceps sinensis powder raw material in botanical drug for strengthening vital qi and removing blood stasis, comprising the steps that: (1) cordyceps sinensis powder is extracted: 0.100g of cordyceps sinensis powder is taken, purified water is added, the ultrasonic extraction, the filtration and the sample injection are carried out; (2) the gradient elution with mobile phase is carried out: octadecyl silane bonded silica gel is taken as a filler, water and acetonitrile are taken as mobile phase to carry out the gradient elution for 0 to 30min and 0 to 7 percent B; (3) a standard fingerprint spectrum is established: the HPLC standard fingerprint spectrum of the cordyceps sinensis powder is determined, and 3 characteristic peaks are selected; (4) the quality control of the fingerprint spectrum is carried out: the relative retention time of No.2 peak uridine, No.3 peak guanosine and No.4 peak adenosine are 0.44 plus or minus 0.03, 0.68 plus or minus 0.03 and 1.00 respectively; the HPLC fingerprint spectrum of the sample is compared with the contrast fingerprint spectrum. The similarity calculated by the 5 common peaks is not less than 0.9, (5) the preparation of the cordyceps sinensis powder raw material is carried out; the control method has good repetitivity and can fully reflect the basic characteristics of nucleoside ingredients of the cordyceps sinensis powder.

A health-care food for improving immunity is prepared from propolis, ganoderma extract and cordyceps. Its preparing process is also disclosed.

The invention relates to the field of biological healthcare foods, in particular to a granular preparation prepared from ultramicro pure cordycep sporocarp powder and a preparation method thereof. Cordycep sporocarps and pure water are taken as raw materials; and the grain size of the ultramicro pure cordycep sporocarp powder is within 200-500nm. The preparation method comprises the following steps of: crushing cordycep sporocarp dried products to be ultramicro powders with grain sizes of 200-500nm, further granulating the prepared ultramicro powders through a granulating machine (experiment machine type is a DWY-5 type multi-kinetic-energy one-step granulating machine), sieving the prepared grains to be grain sizes within 250-1700mciron, and obtaining ultramicro pure cordycep sporocarp powder granular preparations. In the preparation method disclosed by the invention, any auxiliary granulation raw materials do not need to be added, thus a process of pure-material pure-preparation is realized, the production and process costs of the granular products are largely reduced at the same time, and the purity of products is guaranteed and improved; in addition, the invention has the advantages of simple application and operation and easiness in popularization.

The invention relates to the field of health-caring foods and particularly relates to cordyceps sinensis liquor, a preparation and an application thereof. A preparation method of the cordyceps sinensis liquor includes following steps: cultivating sporocarps of cordyceps sinensis in a container through a solid fermentation method and then filling basic liquor into the container with storage. The cordyceps sinensis liquor is prepared through a novel technology, in which activated components in a culture medium and cultivated products are completely retained, instead of a conventional technology in which the clear cordyceps sinensis liquor is prepared with the cordyceps sinensis as a raw material and through a series of complex technologies including filtration, distillation and blending and the like. The cordyceps sinensis liquor is good in appearance, is mellow and delicious, maintains the main activated components, is beneficial to human health and is excellent in market prospect.