Qi-tonifying and blood-activating dietary therapy formula based on traditional Chinese medicine Qi-blood theory and processing technology

Abstract

The invention relates to the field of functional food processing, and discloses a qi-tonifying and blood-activating dietary therapy formula based on the theory of qi and blood in traditional Chinese medicine, which comprises astragalus membranaceus, angelica sinensis, red dates, lycium barbarum, lycium ruthenicum, ginseng, salvia miltiorrhiza, mulberry, lucid ganoderma, cordyceps mycelium, rhodiola rosea, American ginseng, acanthopanax, grape seed extract and chrysanthemum. Through a compound formula based on the theory of qi and blood in traditional Chinese medicine, and by combining various core medicinal materials and functional plants such as the astragalus membranaceus, the angelica sinensis, the ginseng, the lucid ganoderma, the rhodiola rosea and the fructus lycii, the multi-level effects of tonifying qi, enriching blood, activating blood, resisting oxidation and regulating immunity are achieved. The technical effects of comprehensively regulating qi and blood and improving body immunity and resistance are achieved through the synergistic effect of the compound components, compared with a scheme of regulating qi and blood by depending on a single component in the prior art, the compound formula remarkably improves the overall drug effect, solves the problems that a single component is limited in regulating function and insufficient in curative effect, and is suitable for popularization and application. Therefore, the requirements of modern people on comprehensive conditioning and immunity enhancement are met.

Description

Technical Field

[0001] The invention relates to the technical field of functional food processing, in particular to a qi-invigorating and blood-activating food therapy formula and a processing technology based on the qi-blood theory of traditional Chinese medicine. Background Art

[0002] According to the Qi and Blood Theory of Traditional Chinese Medicine, the health of the human body depends on the fullness and circulation of Qi and blood. Qi is the driving force of life activities, while blood is the material basis for nourishing and moisturizing the body. The balance and coordination of Qi and blood can maintain the normal physiological functions of the human body. However, with the accelerated pace of life and increased pressure in modern society, bad living habits, unbalanced diet and other factors have caused many people to suffer from symptoms such as Qi deficiency, blood deficiency, Qi stagnation, and blood stasis, which manifest as health problems such as low immunity, fatigue, exhaustion, and poor circulation. For this reason, Qi-invigorating and blood-activating food therapy products that regulate Qi and blood, enhance immunity, and relieve fatigue have gradually attracted people's attention.

[0003] In the prior art, most of the dietary products for invigorating qi and promoting blood circulation use single or a few Chinese medicinal materials such as astragalus, ginseng, and angelica as the main ingredients. Although these ingredients have certain effects of invigorating qi, nourishing blood, or promoting blood circulation, the effects of a single ingredient are often limited to one aspect, and it is difficult to meet the multiple needs of qi and blood conditioning, immune enhancement, anti-oxidation, and anti-fatigue. In addition, the efficacy of a single ingredient is relatively limited, and people with different physiques have different reactions to it, which is difficult to adapt to the needs of modern people for comprehensive conditioning. Therefore, qi-invigorating and blood-activating products with a single ingredient or a simple formula have obvious limitations in function.

[0004] On the other hand, the extraction processes of existing qi-invigorating and blood-activating products mostly use high-temperature decoction or single water extraction, alcohol extraction and other methods, which have significant deficiencies in extracting active ingredients. High-temperature decoction can easily lead to the destruction of the activity of some heat-sensitive ingredients (such as astragalus polysaccharides, ginsenosides, ganoderma triterpenes, etc.), thereby affecting the final efficacy of the product. At the same time, traditional extraction processes are difficult to effectively separate fat-soluble and water-soluble components, resulting in insufficient product purity and stability, which in turn affects its overall efficacy.

[0005] In addition, products that invigorate qi and activate blood circulation contain active ingredients with macromolecular structures such as polysaccharides and saponins. Due to their large molecular weight, they are inefficiently absorbed by the human body and have low utilization rates. Existing dietary products for regulating qi and blood usually directly use extracted macromolecular components without further decomposition, resulting in poor absorption of the products in actual applications, making it difficult to achieve the ideal conditioning effect, and affecting the actual efficacy of the products.

[0006] Most of the existing Qi-invigorating and blood-activating products are single-layer release dosage forms, such as pills and tablets. After taking them, the active ingredients are released quickly. Although they take effect quickly, the efficacy is difficult to last, and they cannot meet the needs of users for long-term conditioning. The rapid release form will also lead to faster metabolism of the active ingredients in the body, and the efficacy will be short-lived, making it difficult to provide users with a long-term Qi and blood conditioning effect.

[0007] Therefore, the present invention proposes a Qi-invigorating and blood-activating dietary therapy formula and a processing technology based on the Qi-blood theory of traditional Chinese medicine to overcome the deficiencies of the prior art. Summary of the invention

[0008] In view of the shortcomings of the prior art, the present invention provides a Qi-invigorating and blood-activating dietary therapy formula based on the Qi and blood theory of Traditional Chinese Medicine and its processing technology, which solves the technical difficulties that the existing Qi-invigorating and blood-activating products cannot meet the needs of comprehensive Qi and blood conditioning, immune enhancement and long-term conditioning due to single ingredients, backward extraction technology, low bioavailability, and short drug efficacy maintenance time.

[0009] To achieve the above purpose, the present invention is implemented by the following technical scheme: a dietary formula for invigorating qi and promoting blood circulation based on the theory of qi and blood in traditional Chinese medicine, the formula comprising the following ingredients in percentage by weight:

[0010] Astragalus: 15-20%

[0011] Angelica: 10-15%

[0012] Red dates: 8-12%

[0013] Wolfberry and black wolfberry: 5-10%

[0014] Ginseng: 5-8%

[0015] Salvia miltiorrhiza: 5-10%

[0016] Mulberries: 5-8%

[0017] Lingzhi: 3-5%

[0018] Cordyceps mycelium: 2-5%

[0019] Rhodiola rosea: 3-5%

[0020] American ginseng: 2-4%

[0021] Acanthopanax: 2-4%

[0022] Grape seed extract: 1-3%

[0023] Chrysanthemum: 2-3%.

[0024] Preferably, the processing technology of the Qi-invigorating and blood-activating dietary therapy formula based on the Qi-blood theory of traditional Chinese medicine comprises the following steps:

[0025] S1, the fat-soluble ingredients of astragalus, ginseng, ganoderma lucidum and rhodiola rosea in the formula were treated by supercritical CO 2 Extraction To extract;

[0026] S2, extracting the water-soluble components of astragalus, angelica and cordyceps mycelium in the formula by microwave-assisted extraction;

[0027] S3, fermenting the extracts of Ganoderma lucidum, Cordyceps mycelium and American ginseng;

[0028] S4, enzymatically hydrolyzing the polysaccharide and protein extracts;

[0029] S5, performing nano-treatment on all extracts;

[0030] S6. Use multi-layer microencapsulation technology to encapsulate the nano-sized extract.

[0031] Preferably, the supercritical CO 2 The specific steps of extraction include:

[0032] Astragalus, ginseng, ganoderma lucidum, and rhodiola rosea were chopped and loaded into supercritical CO 2 In extraction equipment;

[0033] The extraction parameters are: temperature 35-45°C, pressure 15-25MPa, time 30-60 minutes;

[0034] The fat-soluble components obtained by extraction include astragaloside, ginsenoside and ganoderma triterpenoids, and the extract is collected after filtration for compounding.

[0035] Preferably, the specific steps of the microwave-assisted extraction include:

[0036] Astragalus, Chinese angelica, and Cordyceps mycelium were chopped into small pieces, mixed with purified water at a weight ratio of 1:10, and placed in a microwave-assisted extraction device;

[0037] The extraction conditions are: power 500-700W, temperature 80-90℃, time 10-20 minutes;

[0038] The water-soluble components obtained by extraction include astragalus polysaccharide, angelica polysaccharide and cordyceps polysaccharide. The extract is collected after filtration for compounding.

[0039] Preferably, the specific steps of the fermentation treatment include:

[0040] placing the extract of ganoderma lucidum, cordyceps mycelium and American ginseng in a fermentation tank;

[0041] Add lactic acid bacteria and bifidobacteria, and ferment at 35-40℃ for 24-48 hours;

[0042] The small molecule active ingredients produced during the fermentation process include Ganoderma lucidum polysaccharides, American ginseng saponins, etc., which are used to improve the biological activity and absorption rate of the extract. After fermentation, the fermentation liquid is filtered and collected.

[0043] Preferably, the nano-processing step comprises:

[0044] The composite extract is placed under a pressure of 60-80 MPa using a high-pressure homogenization device and circulated for 3-5 times to reduce the particle size of the extract to less than 100 nm;

[0045] The obtained nano-extract is used to enhance the absorption efficiency in the human body and improve the stability of the components.

[0046] Preferably, the specific steps of the enzymatic treatment include:

[0047] Place the polysaccharide and protein extracts in an enzymatic hydrolysis device, add cellulase and protease, adjust the temperature to 50-60°C, and the pH value to 5.5-6.5;

[0048] The enzymatic hydrolysis time is 1-2 hours, and the obtained small molecule active components include oligosaccharides and small molecule proteins. After the enzymatic hydrolysis, the enzymatic hydrolyzate is filtered and collected.

[0049] Preferably, the specific steps of the multi-layer microcapsule technology include:

[0050] The nano-sized extract is mixed with 1.5-2.0% sodium alginate solution, and the outer layer of quick-release microcapsules is formed by spray drying technology;

[0051] The quick-release microcapsules are mixed with 0.5-1.0% chitosan solution and spray-dried to form middle-layer sustained-release microcapsules;

[0052] Finally, the middle-layer sustained-release microcapsules are mixed with natural lipids, and spray drying technology is used to form inner-layer long-acting sustained-release microcapsules to achieve multi-layer and staged release of active ingredients.

[0053] Preferably, the Qi-invigorating and blood-activating dietary therapy formula is finally prepared into a variety of dosage forms, including instant powder, buccal tablets and functional drinks, to meet the needs of different users, wherein:

[0054] The instant powder is prepared by spray drying process, with air inlet temperature of 180-200℃ and air outlet temperature of 80-90℃;

[0055] The buccal tablets are prepared by mixing the microcapsule powder with mannitol and microcrystalline cellulose in a certain proportion and compressing them into tablets;

[0056] Functional drinks are prepared by mixing fermented extract with a sugar-free juice or purified water base.

[0057] The present invention provides a dietary formula and processing technology for invigorating qi and promoting blood circulation based on the qi and blood theory of traditional Chinese medicine. It has the following beneficial effects:

[0058] 1. The present invention aims to achieve multi-level effects of invigorating qi, nourishing blood, promoting blood circulation, anti-oxidation and immunomodulation through a composite formula based on the theory of qi and blood in traditional Chinese medicine, combined with a variety of core medicinal materials and functional plants such as astragalus, angelica, ginseng, ganoderma lucidum, rhodiola rosea, and wolfberry. The synergistic effect of the composite ingredients achieves the technical effect of comprehensively regulating qi and blood, and improving the body's immunity and resistance. Compared with the scheme of the prior art that relies on a single ingredient for qi and blood conditioning, the composite formula of the present invention significantly improves the overall efficacy, and solves the problem of limited conditioning function and insufficient efficacy of a single ingredient, thereby meeting the needs of modern people for comprehensive conditioning and immune enhancement.

[0059] 2. The present invention innovatively combines supercritical CO 2 The extraction and microwave-assisted extraction processes target fat-soluble components and water-soluble components respectively, ensuring that the core active ingredients are extracted under low temperature and optimal conditions. This process retains the high activity and high purity of the Qi-invigorating and blood-activating components, achieving the technical effect of improving biological activity and enhancing product stability. Compared with the traditional high-temperature decoction extraction method, the multiple extraction technology of the present invention effectively reduces the problem of high temperature damage to the activity of the ingredients, ensures the stability and activity of the ingredients, and greatly improves the problem of loss of active ingredients caused by high temperature in the prior art.

[0060] 3. The present invention uses fermentation and enzymolysis to convert ingredients such as Ganoderma lucidum, Cordyceps mycelium and American ginseng into active substances such as small molecule polysaccharides, saponins and small molecule proteins under the action of fermentation and enzymolysis, which greatly improves the absorption rate of the human body and achieves the technical effect of optimizing the utilization rate of ingredients and enhancing the efficacy. The fermentation and enzymolysis process effectively solves the technical problem of low absorption rate of macromolecular ingredients in the prior art, ensuring that the Qi-invigorating and blood-activating ingredients in the formula can be more efficiently utilized and absorbed, so that the efficacy of the medicine is maximized in the body, providing users with a more significant Qi-invigorating and blood-activating effect.

[0061] 4. The present invention uses nano-processing and multi-layer microcapsule encapsulation technology to design and encapsulate the core ingredients according to the quick-release layer, sustained-release layer and long-acting layer, achieving the effect of releasing the effective ingredients in stages in the body. Through the layered structure of quick-release, sustained-release and long-acting release, the long-term and sustained release of the drug effect is achieved, ensuring that the Qi-invigorating and blood-activating components can play a long-term and stable role in the body. Compared with the problems of uncontrollable component release speed and short drug effect maintenance time in the prior art, the staged release technology of the present invention effectively solves the problem of too fast or uneven component release, ensures the continuity and stability of the drug effect, and provides users with a long-lasting and stable Qi-invigorating and blood-activating conditioning effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0062] Figure 1 This is a processing flow chart for a dietary therapy formula that replenishes qi and promotes blood circulation based on the theory of qi and blood in traditional Chinese medicine. DETAILED DESCRIPTION

[0063] The technical solution of the present invention will be described clearly and completely below in conjunction with the accompanying drawings of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0064] Please refer to the attached Figure 1 :

[0065] Embodiment 1:

[0066] Formula composition (by weight percentage):

[0067] Astragalus: 18%

[0068] Angelica: 12%

[0069] Red dates: 10%

[0070] Wolfberry and black wolfberry: 7%

[0071] Ginseng: 6%

[0072] Salvia miltiorrhiza: 8%

[0073] Mulberries: 6%

[0074] Lingzhi: 4%

[0075] Cordyceps mycelium: 3%

[0076] Rhodiola rosea: 4%

[0077] American ginseng: 3%

[0078] Acanthopanax: 3%

[0079] Grape seed extract: 2%

[0080] Chrysanthemum: 2%

[0081] Preparation process parameters:

[0082] Supercritical CO 2 extraction

[0083] Temperature: 40℃ Pressure: 20MPa

[0084] Duration: 45 minutes

[0085] Microwave assisted extraction:

[0086] Power: 600W

[0087] Temperature: 85℃ Time: 15 minutes

[0088] Fermentation treatment:

[0089] Fermentation bacteria: lactic acid bacteria and bifidobacteria Temperature: 37°C Time: 36 hours

[0090] Enzymatic treatment:

[0091] Enzyme: Cellulase, Protease Temperature: 55℃

[0092] pH: 6.0

[0093] Time: 1.5 hours

[0094] Nano-processing:

[0095] Pressure: 70MPa

[0096] Cycle times: 4 times Particle size: less than 100nm

[0097] Multi-layer microcapsule packaging:

[0098] Immediate release layer: sodium alginate concentration 1.7%

[0099] Sustained release layer: chitosan concentration 0.7%

[0100] Long-acting layer: natural lipid wrapping, spray drying to form a multi-layer microcapsule structure

[0101] Summary of embodiments:

[0102] In this embodiment, the ratio of ingredients and process parameters are within the recommended range, ensuring the overall activity and stability of the formula. Through reasonable supercritical extraction and microwave-assisted extraction, the biological activity of ingredients such as astragaloside and ganoderma triterpenoids can be effectively extracted and maintained. Fermentation and enzymolysis further improve the absorption rate of macromolecular components. Nano- and multi-layer microcapsule encapsulation achieves rapid absorption and long-term release of ingredients, which is suitable for user groups that require stable and continuous conditioning effects.

[0103] Embodiment 2:

[0104] Formula composition (by weight percentage):

[0105] Astragalus: 15%

[0106] Angelica: 10%

[0107] Red dates: 8%

[0108] Wolfberry and black wolfberry: 5%

[0109] Ginseng: 5%

[0110] Salvia miltiorrhiza: 5%

[0111] Mulberry: 5%

[0112] Lingzhi: 3%

[0113] Cordyceps mycelium: 2%

[0114] Rhodiola rosea: 3%

[0115] American ginseng: 2%

[0116] Acanthopanax: 2%

[0117] Grape seed extract: 1%

[0118] Chrysanthemum: 2%

[0119] Preparation process parameters:

[0120] Supercritical CO 2 extraction

[0121] Temperature: 35℃ Pressure: 15MPa

[0122] Time: 30 minutes

[0123] Microwave assisted extraction:

[0124] Power: 500W

[0125] Temperature: 80℃ Time: 10 minutes

[0126] Fermentation treatment:

[0127] Fermentation strain: lactic acid bacteria Temperature: 35℃ Time: 24 hours

[0128] Enzymatic treatment:

[0129] Enzyme type: Cellulase Temperature: 50℃

[0130] pH: 5.5

[0131] Time: 1 hour

[0132] Nano-processing:

[0133] Pressure: 60MPa

[0134] Cycle times: 3 times Particle size: close to 100nm

[0135] Multi-layer microcapsule package Quick release layer: Sodium alginate concentration 1.5% Sustained release layer: Chitosan concentration 0.5%

[0136] Long-lasting layer: natural lipid wrapping

[0137] Summary of embodiments:

[0138] In this example, the ratio of ingredients and process parameters are set at the lower limit of the recommended range to reduce the overall concentration and processing intensity, resulting in a milder conditioning effect. It is suitable for users who are first-time users or are sensitive to ingredients. Through a mild process and a lower extraction temperature, most of the active ingredients are retained, but the activity intensity and sustained effect are slightly weaker than in Example 1. This example emphasizes gentleness and is more suitable for people with weak constitutions who need mild conditioning.

[0139] Embodiment 3:

[0140] Formula composition (by weight percentage):

[0141] Astragalus: 20%

[0142] Angelica: 15%

[0143] Red dates: 12%

[0144] Wolfberry and black wolfberry: 10%

[0145] Ginseng: 8%

[0146] Salvia miltiorrhiza: 10%

[0147] Mulberries: 8%

[0148] Lingzhi: 5%

[0149] Cordyceps mycelium: 5%

[0150] Rhodiola rosea: 5%

[0151] American ginseng: 4%

[0152] Acanthopanax: 4%

[0153] Grape seed extract: 3%

[0154] Chrysanthemum: 3%

[0155] Preparation process parameters:

[0156] Supercritical CO 2 extraction

[0157] Temperature: 45℃ Pressure: 25MPa

[0158] Duration: 60 minutes

[0159] Microwave assisted extraction:

[0160] Power: 700W

[0161] Temperature: 90℃ Time: 20 minutes

[0162] Fermentation treatment:

[0163] Fermentation bacteria: lactic acid bacteria, bifidobacterium Temperature: 40℃ Time: 48 hours

[0164] Enzymatic treatment:

[0165] Enzyme: Cellulase, Protease Temperature: 60℃

[0166] pH: 6.5

[0167] Time: 2 hours

[0168] Nano-processing:

[0169] Pressure: 80MPa

[0170] Cycle times: 5 times Particle size: less than 80nm

[0171] Multi-layer microcapsule packaging:

[0172] Immediate release layer: Sodium alginate concentration 2.0% Sustained release layer: Chitosan concentration 1.0%

[0173] Long-lasting layer: natural lipid wrapping

[0174] Summary of embodiments:

[0175] This embodiment achieves a higher active ingredient concentration and bioavailability by increasing the ingredient concentration and process intensity, and is suitable for users with higher requirements for product efficacy. Higher fermentation and enzymatic treatment conditions ensure the full decomposition of macromolecular active substances and greatly improve the absorption rate. The enhanced design of nano- and multi-layer microcapsule encapsulation achieves long-term drug release, which is suitable for groups that need efficient conditioning. However, higher concentrations and process intensity also make the product slightly more irritating, and it is not suitable for people with sensitive constitutions.

[0176] Embodiment 4:

[0177] Formula composition (by weight percentage):

[0178] Astragalus: 17%

[0179] Angelica: 11%

[0180] Red dates: 9%

[0181] Wolfberry and black wolfberry: 7%

[0182] Ginseng: 6%

[0183] Salvia miltiorrhiza: 8%

[0184] Mulberries: 6%

[0185] Lingzhi: 3%

[0186] Cordyceps mycelium: 3%

[0187] Rhodiola rosea: 3%

[0188] American ginseng: 2%

[0189] Acanthopanax: 2%

[0190] Grape seed extract: 4%

[0191] Chrysanthemum: 5%

[0192] Preparation process parameters (same as Example 1):

[0193] Summary of embodiments:

[0194] In this embodiment, the proportion of grape seed extract and chrysanthemum is increased, highlighting the antioxidant and immune enhancement effects. The process parameter selection is the same as that in Example 1, ensuring that the formula enhances the antioxidant effect while maintaining the activity of the ingredients, and is suitable for users who need to improve immunity and antioxidant needs. The increase in antioxidant ingredients in this formula has a protective effect on the immune system and is suitable for people with low immunity or susceptible people.

[0195] Embodiment 5:

[0196] Formula composition (by weight percentage):

[0197] Astragalus: 18%

[0198] Angelica: 12%

[0199] Red dates: 10%

[0200] Wolfberry and black wolfberry: 7%

[0201] Ginseng: 6%

[0202] Salvia miltiorrhiza: 8%

[0203] Mulberries: 6%

[0204] Lingzhi: 4%

[0205] Cordyceps mycelium: 3%

[0206] Rhodiola rosea: 4%

[0207] American ginseng: 3%

[0208] Acanthopanax: 3%

[0209] Grape seed extract: 2%

[0210] Chrysanthemum: 2%

[0211] Preparation process parameters:

[0212] Supercritical CO 2 extraction

[0213] Temperature: 38℃

[0214] Pressure: 18MPa

[0215] Duration: 40 minutes

[0216] Microwave assisted extraction:

[0217] Power: 600W

[0218] Temperature: 85℃

[0219] Time: 15 minutes

[0220] Fermentation treatment:

[0221] Fermentation bacteria: Bifidobacterium

[0222] Temperature: 37℃

[0223] Time: 36 hours

[0224] Enzymatic treatment:

[0225] Enzyme: Cellulase

[0226] Temperature: 55℃

[0227] pH: 6.0

[0228] Time: 1.5 hours

[0229] Nano-processing:

[0230] Pressure: 75MPa

[0231] Number of cycles: 4 times

[0232] Particle size: less than 100nm

[0233] Multi-layer microcapsule encapsulation

[0234] Immediate release layer: sodium alginate concentration 1.8%

[0235] Sustained release layer: chitosan concentration 0.8%

[0236] Long-lasting layer: natural lipids

[0237] Summary of embodiments:

[0238] In this embodiment, by adjusting the pressure and number of cycles of the nano-treatment, the molecular size of the macromolecular active ingredients is optimized and the absorption efficiency of the ingredients is enhanced. It is suitable for users who have high demands for absorption rate and need to see results quickly. The relatively neutral formula concentration and moderate process parameters ensure the mildness and high bioavailability of the product.

[0239] Comparative experiment 1: Ingredient missing comparison experiment

[0240] Experimental design and process:

[0241] The purpose of this experiment is to verify the role of the antioxidant and immune-enhancing components in the composite formula of the present invention in regulating qi and blood and immunity. To this end, the differences in related efficacy between the single component or a few component formulas lacking these innovative components in the prior art and the composite component formula of the present invention are compared.

[0242] Experimental group (the scheme of the present invention): the composite formula of the present invention is adopted, which includes basic ingredients such as astragalus, angelica, ginseng, and ganoderma lucidum, and innovative ingredients such as rhodiola, black wolfberry, American ginseng, Acanthopanax, grape seed extract and chrysanthemum are added. It is expected to provide multiple effects of invigorating qi and promoting blood circulation, anti-oxidation, and enhancing immunity.

[0243] Control group (existing technology solution): using the existing technology formula, containing only basic Qi-invigorating and blood-activating ingredients such as astragalus, angelica, ginseng and ganoderma, and no innovative ingredients for antioxidant and immune enhancement.

[0244] Each group of subjects took the same amount of the experimental group and control group formula every day for 30 days. The following key indicators were measured before and after the test: Qi and blood indicators (red blood cell count, hemoglobin content), immune indicators (white blood cell count) and antioxidant capacity (superoxide dismutase SOD activity), and fatigue scores were recorded.

[0245] Comparative experimental data

[0246]

[0247] Result analysis:

[0248] Experimental data show that the Qi and blood and immune effects of the composite formula of the present invention are significantly better than those of the control group. The specific reasons are as follows:

[0249] The effect of regulating qi and blood is significantly improved: the red blood cell count and hemoglobin content in the experimental group are significantly higher than those in the control group. This is because the rhodiola rosea and black wolfberry introduced in the present invention have the effect of activating blood circulation and promoting qi and blood circulation, which can more effectively stimulate hematopoietic function and enhance the production of red blood cells and hemoglobin, thereby achieving a better effect of regulating qi and blood. The control group formula lacks these ingredients, and the effect of qi and blood production is relatively insufficient.

[0250] Antioxidant and immune enhancement: The composite formula of the present invention adds antioxidant ingredients (such as grape seed extract and chrysanthemum) and immunomodulatory ingredients (such as Acanthopanax senticosus and American ginseng), which significantly improves the SOD activity to 160U / mL, which is 23% higher than the control group. These antioxidant ingredients can effectively reduce oxidative stress, slow down cell aging, and further enhance immunity by removing free radicals in the body. Relatively speaking, the control group lacks antioxidant ingredients, resulting in lower SOD activity in the body and insignificant immune indicators.

[0251] Significant fatigue relief effect: The fatigue score of the experimental group decreased by 8 points, indicating a significant fatigue relief effect. This is because the ingredients such as Rhodiola rosea and Acanthopanax senticosus contained in the composite formula of the present invention have anti-fatigue effects, which effectively improve fatigue symptoms by enhancing cell vitality and improving body endurance. The control group formula lacks these ingredients, resulting in a weak fatigue relief effect, which cannot meet the user's demand for rapid fatigue elimination.

[0252] In summary, the scheme of the present invention has achieved significant improvement over the prior art through the combination of innovative ingredients, especially in the aspects of qi and blood conditioning, immune enhancement and anti-fatigue, which verifies the scientificity and effect of the composite formula of the present invention.

[0253] Comparative experiment 2: Comparative experiment of multi-ingredient combination ratio

[0254] Experimental design and procedures

[0255] The purpose of this experiment is to further verify the effect of the combination of antioxidant ingredients and immune enhancement ingredients in the composite formula of the present invention in enhancing immunity and anti-fatigue. Compare the differences between the prior art formula lacking antioxidant ingredients and the complete composite formula of the present invention.

[0256] Experimental group (the scheme of the present invention): using the composite formula of the present invention, comprising basic ingredients such as astragalus, ginseng, angelica, and ganoderma lucidum, and adding antioxidant and immune-enhancing ingredients such as rhodiola, grape seed extract, and Acanthopanax senticosus.

[0257] The control group (existing technology solution) used traditional Qi-invigorating and blood-activating ingredients, including astragalus, angelica, ginseng and ganoderma lucidum, but did not contain the antioxidant ingredients rhodiola rosea and grape seed extract.

[0258] After each group of subjects took the medicine daily for 30 days, key anti-fatigue and immune indicators such as superoxide dismutase (SOD) activity, white blood cell count, and fatigue recovery time were measured.

[0259] Comparative experimental data:

[0260]

[0261] Result analysis:

[0262] The experimental results show that the solution of the present invention with added antioxidant ingredients is significantly better than the control group in terms of enhancing immunity and anti-fatigue effects. The specific analysis is as follows:

[0263] Significantly improve the antioxidant capacity: The SOD activity of the experimental group reached 170U / mL, which was 36% higher than that of the control group. This shows that the ingredients such as grape seed extract and rhodiola rosea in the present invention have a significant effect on antioxidants. The higher the SOD activity, the stronger the body's antioxidant capacity and the better the free radical scavenging effect, thereby reducing the damage of oxidative stress to cells. Therefore, the scheme of the present invention has significant advantages in reducing oxidative damage and protecting cell health, while the control group has a low SOD activity and weak antioxidant capacity due to the lack of antioxidant ingredients.

[0264] Enhanced immunity and endurance: The increase in white blood cell counts indicates that the experimental group has a better immune enhancement effect than the control group, which is due to the immunomodulatory effects of ingredients such as Rhodiola rosea and Acanthopanax senticosus, which can improve the body's immune activity and enhance resistance to foreign pathogens. The control group formula lacks these ingredients, so the immune enhancement effect is not obvious. The shortened fatigue recovery time further verifies the anti-fatigue effect of the formula of the present invention, which is mainly due to the synergistic effect of ingredients such as Acanthopanax senticosus and Rhodiola rosea, which restores vitality and endurance in a short time.

[0265] By adding antioxidant and immune-enhancing ingredients, the scheme of the present invention significantly improves the immune and anti-fatigue effects, and is particularly suitable for people with low immunity or prone to fatigue, verifying the scientific nature and superiority of the composite formula design.

[0266] Comparative experiment 3: Processing technology comparison experiment

[0267] Experimental design and process:

[0268] The purpose of this experiment is to verify the effects of the innovative processing technologies of the present invention such as multiple extraction, fermentation and enzymatic hydrolysis in improving the activity and absorption rate of ingredients. The differences in the activity retention and absorption rate of ingredients under the high-temperature decoction extraction process in the prior art and the multiple extraction process of the present invention are compared.

[0269] Experimental group (the present invention): using the multiple extraction process of the present invention, including supercritical CO 2 extraction, microwave-assisted extraction, and processed by fermentation and enzymatic hydrolysis.

[0270] Control group (existing technology solution): using the traditional high-temperature decoction extraction process to extract the main active ingredients, without using biological treatment technologies such as fermentation or enzymatic hydrolysis.

[0271] Under the same ingredient ratio and dosage conditions, the two groups of products were tested for ingredient content and in vivo absorption rate, and the astragalus polysaccharide content, Ganoderma lucidum triterpenoid content, absorption rate and fatigue score were recorded respectively.

[0272] Comparative experimental data:

[0273]

[0274] Result analysis:

[0275] The solution of the present invention significantly improves the retention rate and in vivo absorption rate of the active ingredients. The specific reasons are analyzed as follows:

[0276] The retention rate of active ingredients was significantly improved: the contents of astragalus polysaccharide and ganoderma triterpenoid in the experimental group reached 22 mg / g and 9 mg / g, respectively, which were 46% and 80% higher than those in the control group. 2 The microwave-assisted extraction process effectively retained these heat-sensitive components at a lower temperature, avoiding the problem of activity loss caused by excessive temperature during traditional high-temperature decoction. In contrast, the control group lost some polysaccharides and triterpenes due to high-temperature treatment, and the activity retention rate was lower.

[0277] Absorption rate significantly improved: In the scheme of the present invention, polysaccharides and protein components are fermented and enzymatically treated to be broken down into small molecular structures, so that their absorption rate reaches 85%, which is much higher than the 60% of the control group. This process makes large molecular active ingredients easier for the human body to absorb, significantly improving bioavailability. However, due to the lack of fermentation and enzymatic treatment, the absorption of active ingredients in the control group is not ideal, and the actual therapeutic effect is limited.

[0278] Enhanced fatigue relief effect: Due to the efficient absorption of ingredients, the fatigue score of the experimental group was reduced by 7 points, showing a more significant fatigue relief effect. This is mainly due to the enhanced absorption of polysaccharides and triterpenes, which played a stronger role in physical recovery.

[0279] In summary, the multiple extraction, fermentation and enzymatic hydrolysis processes of the present invention effectively solve the problems of active ingredient loss and low absorption rate in the prior art, greatly improve the efficacy and bioavailability of the product, and provide users with more significant health effects.

[0280] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A dietary formula for invigorating qi and promoting blood circulation based on the theory of qi and blood in traditional Chinese medicine, characterized in that: The formulation contains the following ingredients in percentage by weight: Astragalus: 15-20% Angelica: 10-15% Red dates: 8-12% Wolfberry and black wolfberry: 5-10% Ginseng: 5-8% Salvia miltiorrhiza: 5-10% Mulberries: 5-8% Lingzhi: 3-5% Cordyceps mycelium: 2-5% Rhodiola rosea: 3-5% American ginseng: 2-4% Acanthopanax: 2-4% Grape seed extract: 1-3% Chrysanthemum: 2-3%.

2. The processing technology of the Qi-invigorating and blood-activating dietary therapy formula based on the Qi-blood theory of traditional Chinese medicine according to claim 1 is characterized in that: The processing technology comprises the following steps: S1. Extracting the fat-soluble components of astragalus, ginseng, ganoderma lucidum and rhodiola rosea in the formula by supercritical CO2 extraction; S2, extracting the water-soluble components of astragalus, angelica and cordyceps mycelium in the formula by microwave-assisted extraction; S3, fermenting the extracts of Ganoderma lucidum, Cordyceps mycelium and American ginseng; S4, enzymatically hydrolyzing the polysaccharide and protein extracts; S5, performing nano-treatment on all extracts; S6. Use multi-layer microencapsulation technology to encapsulate the nano-sized extract.

3. The processing technology of the Qi-invigorating and blood-activating dietary therapy formula based on the Qi-blood theory of traditional Chinese medicine according to claim 2 is characterized in that: The specific steps of the supercritical CO2 extraction include: Chop the astragalus, ginseng, ganoderma lucidum and rhodiola rosea into small pieces and put them into the supercritical CO2 extraction equipment; The extraction parameters are: temperature 35-45°C, pressure 15-25MPa, time 30-60 minutes; The fat-soluble components obtained by extraction include astragaloside, ginsenoside and ganoderma triterpenoids, and the extract is collected after filtration for compounding.

4. The processing technology of the Qi-invigorating and blood-activating dietary therapy formula based on the Qi-blood theory of traditional Chinese medicine according to claim 2 is characterized in that: The specific steps of the microwave-assisted extraction include: Astragalus, Chinese angelica, and Cordyceps mycelium were chopped into small pieces, mixed with purified water at a weight ratio of 1:10, and placed in a microwave-assisted extraction device; The extraction conditions are: power 500-700W, temperature 80-90℃, time 10-20 minutes; The water-soluble components obtained by extraction include astragalus polysaccharide, angelica polysaccharide and cordyceps polysaccharide. The extract is collected after filtration for compounding.

5. The processing technology of the Qi-invigorating and blood-activating dietary therapy formula based on the Qi-blood theory of traditional Chinese medicine according to claim 2 is characterized in that: The specific steps of the fermentation process include: placing the extract of ganoderma lucidum, cordyceps mycelium and American ginseng in a fermentation tank; Add lactic acid bacteria and bifidobacteria, and ferment at 35-40℃ for 24-48 hours; The small molecule active ingredients produced during the fermentation process include Ganoderma lucidum polysaccharides, American ginseng saponins, etc., which are used to improve the biological activity and absorption rate of the extract. After fermentation, the fermentation liquid is filtered and collected.

6. The processing technology of the Qi-invigorating and blood-activating dietary therapy formula based on the Qi-blood theory of traditional Chinese medicine according to claim 2, characterized in that: The nano-processing step comprises: The composite extract is placed under a pressure of 60-80 MPa using a high-pressure homogenization device and circulated for 3-5 times to reduce the particle size of the extract to less than 100 nm; The obtained nano-extract is used to enhance the absorption efficiency in the human body and improve the stability of the components.

7. The processing technology of the Qi-invigorating and blood-activating dietary therapy formula based on the Qi-blood theory of traditional Chinese medicine according to claim 1, characterized in that: The specific steps of the enzymatic treatment include: Place the polysaccharide and protein extracts in an enzymatic hydrolysis device, add cellulase and protease, adjust the temperature to 50-60°C, and the pH value to 5.5-6.5; The enzymatic hydrolysis time is 1-2 hours, and the obtained small molecule active components include oligosaccharides and small molecule proteins. After the enzymatic hydrolysis, the enzymatic hydrolyzate is filtered and collected.

8. The Qi-invigorating and blood-activating dietary therapy formula and processing technology based on the Qi-blood theory of traditional Chinese medicine according to claim 1, characterized in that: The specific steps of the multi-layer microcapsule technology include: The nano-sized extract is mixed with 1.5-2.0% sodium alginate solution, and the outer layer of quick-release microcapsules is formed by spray drying technology; The quick-release microcapsules are mixed with 0.5-1.0% chitosan solution and spray-dried to form middle-layer sustained-release microcapsules; Finally, the middle-layer sustained-release microcapsules are mixed with natural lipids, and spray drying technology is used to form inner-layer long-acting sustained-release microcapsules to achieve multi-layer and staged release of active ingredients.

9. The Qi-invigorating and blood-activating dietary therapy formula according to claim 1, characterized in that: The formula is finally prepared into a variety of dosage forms, including instant powder, buccal tablets and functional drinks, to meet the needs of different users, including: The instant powder is prepared by spray drying process, with air inlet temperature of 180-200℃ and air outlet temperature of 80-90℃; The buccal tablets are prepared by mixing the microcapsule powder with mannitol and microcrystalline cellulose in a certain proportion and compressing them into tablets; Functional drinks are prepared by mixing fermented extract with a sugar-free juice or purified water base.

Images