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Antioxidant peptide of hairtail fish protein as well as preparation method and uses of antioxidant peptide

A technology for antioxidant peptides and fish protein, which is applied in peptide preparation methods, chemical instruments and methods, and food preparation, can solve the problems of high-activity antioxidant peptides that have not been reported, and achieve good lipid peroxidation inhibition, good scavenging effect

Active Publication Date: 2015-09-23
北京姿美堂生物技术股份有限公司
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Problems solved by technology

[0004] However, the applicant found that the preparation of antioxidant peptides from hairtail fish meat by enzymatic hydrolysis technology is in a blank stage, and the preparation of high-activity antioxidant peptides and their applications using enzymatic hydrolysis products as materials has not been reported.

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  • Antioxidant peptide of hairtail fish protein as well as preparation method and uses of antioxidant peptide
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  • Antioxidant peptide of hairtail fish protein as well as preparation method and uses of antioxidant peptide

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Embodiment Construction

[0022] The present invention will be further described in detail below with reference to the embodiments of the accompanying drawings.

[0023] The preparation method of hairtail fish protein antioxidant peptide, the preparation process is as follows: hairtail fish meat "defatting" enzymatic hydrolysis "enzymatic hydrolysate" ultrafiltration "macroporous resin purification" cation exchange chromatography "gel filtration chromatography" high performance liquid chromatography Preparation of "antioxidant peptides.

[0024] The specific steps are:

[0025] 1) Pretreatment of hairtail and fish meat: Take Japanese hairtail ( Trichiurus japonicus ) The fish meat was processed into a homogenate with a high-speed tissue masher, heated to 85 °C and kept for 10 min, then added isopropanol according to the ratio of material to liquid 1g:4 mL, defatted at 25 °C for 24 h, and then centrifuged at 4 °C and 6000 rpm. Remove isopropyl alcohol for 15 min, and collect the solids of defatted h...

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Abstract

The invention discloses an antioxidant peptide of hairtail fish protein as well as a preparation method and uses of the antioxidant peptide. In particular, hairtail fish is used as raw materials; an enzymatic hydrolysis solution is obtained through degreasing and enzymolysis of protamex and neutral protease; the enzymatic hydrolysis solution is separated and purified through ultra-filtration, purification of macroporous resins, cation exchange resin chromatography, gel column chromatography, and reversed-phase high-performance liquid chromatography so as to obtain the antioxidant peptide Asn-Trp-Asp-Met-Glu-Lys-Ile-Trp, and the ESI-MS determining molecular weight is 1121.25da. The high-activity antioxidant peptide disclosed by the invention has a good scavenging effect on DPPH free radicals, hydroxyl radicals and superoxide anion free radicals; besides, the antioxidant peptide shows a good effect of lipid peroxidation inhibition. The antioxidant peptide has the advantages of safe use, zero toxic or side effects, high oxidative activity and the like, is liable to digest and absorb, and can be used as medicines, health food or food additives, and the like.

Description

technical field [0001] The invention relates to protein antioxidant peptides, in particular to hairtail fish meat protein antioxidant peptides and a preparation method thereof. Background technique [0002] Antioxidants are a class of substances that can weaken or eliminate free radical damage to the human body and protect food from oxidative damage and deterioration. Currently, chemically synthesized antioxidants are widely used in the food industry due to their low price. However, chemically synthesized antioxidants, such as butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), propyl gallate (PG), tert-butylhydroquinone (TBHQ), etc., to varying degrees Damage the liver, kidneys and other organs of the human body. Therefore, some countries and regions have clearly limited or prohibited the use of chemically synthesized antioxidants that have toxic side effects on the human body. Finding efficient and safe natural antioxidants to replace chemically synthesized ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C12P21/06C07K1/36C07K1/34C07K1/20C07K1/18C07K1/16A61K38/08A61P39/06A23L1/29A23L1/305
Inventor 王斌迟长凤邓尚贵孙坤来陈荫
Owner 北京姿美堂生物技术股份有限公司
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