Liquid fermentation culture medium for high-yield antioxidant cordyceps sobolifera mycelium and production method of antioxidant cordyceps sobolifera mycelium granules

A liquid fermentation and mycelium technology, applied in the field of fermentation technology and micropowder technology production, can solve the problems of scarce wild resources, limited natural resources, long growth cycle, etc., and achieve high biomass, strong antioxidant activity and low production cost low effect

Pending Publication Date: 2019-08-16
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing natural cicadae takes at least 5-6 years to grow. It can only be picked once a year from June to August in summer, and the price is 1-10 yuan per plant. Wild resources are scarce and decreasing year by year. Natural resources are limited and growth long cycle

Method used

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  • Liquid fermentation culture medium for high-yield antioxidant cordyceps sobolifera mycelium and production method of antioxidant cordyceps sobolifera mycelium granules
  • Liquid fermentation culture medium for high-yield antioxidant cordyceps sobolifera mycelium and production method of antioxidant cordyceps sobolifera mycelium granules
  • Liquid fermentation culture medium for high-yield antioxidant cordyceps sobolifera mycelium and production method of antioxidant cordyceps sobolifera mycelium granules

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The production process of high-yield antioxidant cicada mycelium granule is as follows: figure 1 shown.

[0037] 1) Strain activation: Aseptically divide the Paecilomyces cicadae cultured on the slant into mung bean size, inoculate it on the PDA slant medium, and culture it at 25°C for 5 days;

[0038]2) Seed liquid preparation: the activated bacteria are aseptically divided into soybean grain size, inoculated into the seed culture liquid (mass fraction sucrose 2%, soluble starch 3%, soybean powder 2%, KH 2 PO 4 0.1%, MgSO 4 ·7H 2 O 0.05%, pH is natural, liquid content is 40%, add 3-5 glass beads to every 250mL Erlenmeyer flask), inoculate 4 pieces in each bottle, and culture at 25°C and 180r / min for 3 days to obtain the first-grade species . Inoculate the cultivated primary species into the same seed culture solution with a volume fraction of 10% aseptically, and cultivate the secondary species under the same conditions as the primary species;

[0039] 3) Prepar...

Embodiment 2

[0049] 1) Strain activation: Paecilomyces cicadae cultured on a slant was aseptically divided into mung bean size, inoculated on PDA slant medium, and cultured at 25° C. for 5 days.

[0050] 2) Preparation of seed liquid: aseptically divide the activated bacteria into soybean grain size, inoculate into seed culture liquid (2% sucrose, 3% soluble starch, 2% soybean powder, KH 2 PO 4 0.1%, MgSO 4 ·7H 2 O 0.05%, pH is natural, liquid content is 40%, add 3-5 glass beads to every 250mL Erlenmeyer flask), inoculate 8 pieces in each bottle, and culture at 25°C and 180r / min for 2 days to obtain the first-grade species ; Inoculate the cultured first-class species into the same seed culture solution with a volume fraction of 10% aseptically, and cultivate the second-class species under the same conditions as the first-class species;

[0051] 3) Preparation of fermentation medium (take the preparation of 1000mL culture medium as an example): Weigh 50g of iron stick yam powder into a ...

Embodiment 3

[0061] 1) Strain activation: Aseptically divide the Paecilomyces cicadae cultured on the slant into mung bean size, inoculate it on the PDA slant medium, and culture it at 25°C for 5 days;

[0062] 2) Seed liquid preparation: the activated bacteria are aseptically divided into soybean grain size, inoculated into the seed culture liquid (mass fraction sucrose 2%, soluble starch 3%, soybean powder 2%, KH 2 PO 4 0.1%, MgSO 4 ·7H 2 O 0.05%, the pH is natural, the liquid content is 40%, add 3-5 glass beads to each 250mL Erlenmeyer flask), inoculate 3 pieces in each bottle, and culture at 25°C and 180r / min for 2 days to obtain the first-grade species . Inoculate the cultured primary species into the same seed culture solution with a volume fraction of 15% aseptically, and cultivate the secondary species under the same conditions as the primary species;

[0063] 3) Preparation of fermentation medium (take the preparation of 1000mL culture medium as an example): Weigh 10g of Tieg...

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Abstract

The invention discloses a liquid fermentation culture medium for a high-yield antioxidant cordyceps sobolifera mycelium and a production method of antioxidant cordyceps sobolifera mycelium granules. The culture medium comprises the following components in percentages by mass: 1-5% of glucose, 1-10% of a high molecular carbon source, 1-10% of a nitrogen source and 0-0.5% of an inorganic salt respectively, distilled water is used as a solvent, and the pH is natural. The high-yield cordyceps sobolifera mycelium is obtained by improved fermentation through the liquid fermentation culture medium for the high-yield antioxidant cordyceps sobolifera mycelium, the biomass content is high, the contents of effective components including polysaccharides and mannitol are high, the antioxidant activityis strong, and the source of health food raw materials is increased; meanwhile, the cordyceps sobolifera mycelium is obtained by a liquid fermentation technology, the production cost is low, the period is short, the limitation of environmental conditions is avoided, and the cordyceps sobolifera mycelium is suitable for large-scale industrial production and popularization; and the high-yield antioxidant cordyceps sobolifera mycelium granules produced by the invention have effects of easiness in absorption and utilization, convenience in carrying and strong nourishing.

Description

technical field [0001] The invention belongs to the production fields of fermentation technology and micropowder technology, and relates to Cordyceps health food, in particular to a liquid fermentation medium for high-yield anti-oxidation cicadae mycelium and a production method of the anti-oxidation cicadae mycelium granule. Background technique [0002] Cicadae (Cordyceps cicadae) is a fungus with both medicinal and edible uses, and it is also one of the traditional Chinese medicinal materials in my country. Researches at home and abroad have found that cicadae has a wide range of pharmacological activities. Cicadae can enhance the immune regulation function of the body, nourish and strengthen the body, improve the nutritional status of the body, relieve heat and analgesia, improve kidney function, anti-radiation, anti-fatigue, anti-stress, anti- Oxidation, antibacterial, antitumor, antiviral, regulating lipid metabolism, lowering blood pressure, lowering blood sugar, anti...

Claims

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Application Information

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IPC IPC(8): C12N1/14A23L31/00A23L33/00A23L2/39C12R1/79
CPCC12N1/14A23L31/00A23L33/00A23L2/39
Inventor 凡军民谢春芹杨雨靖张雪松刘海燕郁宝锋
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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