Preparation method of thermally responsive bladder extracellular matrix bioactive hydrogel

Abstract

The invention discloses a preparation method of a thermally responsive bladder extracellular matrix bioactive hydrogel, relates to the field related to bioactive hydrogel preparation methods, and solves the problems that most of existing methods for obtaining an extracellular matrix (ECM) in the prior art have the defects of insufficient elution and large damage to ECM, and preparation of bladder-derived ECM hydrogels has not been reported so far. The preparation method comprises the following steps: step 1: taking a fresh pig bladder, keeping the ureter and urethral structure, and conductingrinsing with 3000 mL of double distilled water for 2 h; step 2: after wrapping by tin foil paper, quickly place the wrapped object in a refrigerator of -80 DEG C for 1 hour, then taking the object outand balancing the object to room temperature; step 3: repeating the step two for two times; step 4: conducting perfusion and immersing by double distilled water for 4 h, and immersing the whole bladder in a perfusate; and step 5: conducting perfusion and immersing by 1% Triton X-100 for 4 h, and immersing the whole bladder in a 1% Triton X-100 perfusate, and then conducting rinsing by double distilled water for 20 min.

Description

technical field [0001] The invention relates to the related field of preparation methods of bioactive hydrogels, in particular to a preparation method of heat-responsive bladder extracellular matrix bioactive hydrogels. Background technique [0002] Studies in recent years have shown that stem cells have shown good clinical application prospects in promoting vascular endothelial regeneration and repairing bladder smooth muscle damage. Currently, there are two methods of stem cell transplantation for the treatment of bladder injury, namely 1) injection through peripheral blood vessels, including bladder artery and bladder vein injection; 2) local injection, including bladder wall injection. Studies have found that peripheral vascular injection is a more convenient method of stem cell transplantation. However, when peripheral vascular injection, the number of stem cells entering the target organ is small, and it may cause systemic adverse reactions, and the stem cells may stay...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for preparing a heat-responsive bladder extracellular matrix bioactive hydrogel, so as to solve the problem of insufficient elution or greater damage to the ECM in the existing methods for obtaining the extracellular matrix proposed in the background technology. And there are currently no reported issues with the preparation of bladder-derived ECM hydrogels

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