Preparation method and application of instant isatistinctoria leaf extract
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A technology of woad leaves and extracts, which is applied in the field of preparation of instant woad leaves extracts, can solve problems such as poor eyebrow application effect
Inactive Publication Date: 2020-04-21
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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Another storage method is to store it in the sun like tobacco leaves, but the effect of applying eyebrows is not as good as the former
Method used
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Embodiment 1
[0018] a. Freezing 500g of fresh Hotan woad leaves at -80°C for 5 hours;
[0019] B, the frozen woad leaf in step a is pulverized using a pulverizer with cooling;
[0020] c, adding purified water to the pulverized Isatis indigo leaves in the step b according to the weight-to-volume ratio of feed to liquid at 1:3, stirring and extracting for 4 hours, and repeatedly extracting 4 times;
[0021] d. Combine the extracts in step c, filter, 4000r / min, temperature 4°C, centrifuge for 10min, take the supernatant, and freeze-dry to obtain 26.3g of dark green Isatis indica leaf extract freeze-dried powder, place at temperature - Store in refrigerator at 20°C.
Embodiment 2
[0023] a. Freezing 1 kg of fresh Hotan woad leaves at -80°C for 10 hours;
[0024] B, the frozen woad leaf in step a is pulverized using a pulverizer with cooling;
[0025] c, adding purified water to the pulverized Isatis indigo leaves in the step b according to the weight-to-volume ratio of feed to liquid 1:3, stirring and extracting for 4 hours, and repeatedly extracting 3 times;
[0026] d. Combine the extracts in step c, filter, 4000r / min, temperature 4°C, centrifuge for 10min, take the supernatant and freeze-dry to obtain 53g of dark green woad leaf extract freeze-dried powder, place at temperature - Store in refrigerator at 20°C.
Embodiment 3
[0028] a. Freezing 2kg of fresh woad leaves at a temperature of -80°C for 8h;
[0029] B, the frozen Chinese woad leaf in step a is crushed using a pulverizer with a cooling device;
[0030] c, adding purified water to the woad leaves pulverized in step b and adding purified water to stir and extract for 4 hours, and repeatedly extracting 4 times;
[0031] d. Combine the extracts in step c, filter, rotate at 4000r / min, temperature 4°C, centrifuge for 10min, take the supernatant, and freeze-dry to obtain 104g of instant Isatis indigo leaf extract freeze-dried powder, which is placed at a temperature of - Store in refrigerator at 20°C.
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Abstract
The invention relates to a preparation method and application of an instant isatistinctoria leaf extract, in the method, fresh isatistinctoria leaves are used as a raw material, water-soluble substances in the isatistinctoria leaves are extracted by using purified water, and then dry powder is prepared by using a freeze-drying technology. The dry powder obtained through the method is dark green, the fresh isatistinctoria leaves can be converted into a powdery reserve raw material through the method, original active ingredients of the isatistinctoria leaves are reserved to the maximum degree, and the isatistinctoria leaves which are plants which are limited to grow by seasons can be used instantly. The main components of the extract are 31% of polysaccharide and 53% of protein, and in-vitroactivity tests show that the extract has certain antioxidant and antibacterial activity and is applied to preparation of drugs for treating treatment psoriasis or parapsoriasis guttata. The fresh isatistinctoria leaves used in the method are picked and squeezed at present, the original bioactive components of the isatistinctoria leaves are reserved, and the problem that the use of the fresh isatistinctoria leaves is limited by seasons is solved.
Description
technical field [0001] The invention relates to a preparation method and application of an instant woad leaf extract. Background technique [0002] There are about 30 species of Isatis indigo in the world (some taxonomists believe that there are more than 50 species), mainly distributed in Central Asia to the east of the Mediterranean Sea, Iran-Turan area as the distribution center, and eastward to Sakhalin Island and Japan, as far north as Central Europe and North America. There are 6 species and 3 varieties in my country, which are cultivated all over the country. Isatis genus is mainly distributed in Xinjiang, Inner Mongolia and the three northeast provinces in China. Among them, the cultivated species of Isatis native to my country is almost all over the country. This genus is a group with important medical and economic value, in which the dry root of Isatis indica is Radix Isatidis, and the dry leaves are Folium Isatidis. The unique variety of Xinjiang Woad in my coun...
Claims
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