Preparation method and application of instant isatistinctoria leaf extract
A technology of woad leaves and extracts, which is applied in the field of preparation of instant woad leaves extracts, can solve problems such as poor eyebrow application effect
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Embodiment 1
[0018] a. Freezing 500g of fresh Hotan woad leaves at -80°C for 5 hours;
[0019] B, the frozen woad leaf in step a is pulverized using a pulverizer with cooling;
[0020] c, adding purified water to the pulverized Isatis indigo leaves in the step b according to the weight-to-volume ratio of feed to liquid at 1:3, stirring and extracting for 4 hours, and repeatedly extracting 4 times;
[0021] d. Combine the extracts in step c, filter, 4000r / min, temperature 4°C, centrifuge for 10min, take the supernatant, and freeze-dry to obtain 26.3g of dark green Isatis indica leaf extract freeze-dried powder, place at temperature - Store in refrigerator at 20°C.
Embodiment 2
[0023] a. Freezing 1 kg of fresh Hotan woad leaves at -80°C for 10 hours;
[0024] B, the frozen woad leaf in step a is pulverized using a pulverizer with cooling;
[0025] c, adding purified water to the pulverized Isatis indigo leaves in the step b according to the weight-to-volume ratio of feed to liquid 1:3, stirring and extracting for 4 hours, and repeatedly extracting 3 times;
[0026] d. Combine the extracts in step c, filter, 4000r / min, temperature 4°C, centrifuge for 10min, take the supernatant and freeze-dry to obtain 53g of dark green woad leaf extract freeze-dried powder, place at temperature - Store in refrigerator at 20°C.
Embodiment 3
[0028] a. Freezing 2kg of fresh woad leaves at a temperature of -80°C for 8h;
[0029] B, the frozen Chinese woad leaf in step a is crushed using a pulverizer with a cooling device;
[0030] c, adding purified water to the woad leaves pulverized in step b and adding purified water to stir and extract for 4 hours, and repeatedly extracting 4 times;
[0031] d. Combine the extracts in step c, filter, rotate at 4000r / min, temperature 4°C, centrifuge for 10min, take the supernatant, and freeze-dry to obtain 104g of instant Isatis indigo leaf extract freeze-dried powder, which is placed at a temperature of - Store in refrigerator at 20°C.
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