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Substance content determination method and application thereof

A detection method and technology for substances, which are applied in chemical analysis by titration, medical preparations containing active ingredients, and drug combinations, etc., can solve the problems of many operation cost steps, insufficient internal quality of traditional Chinese medicine preparations, etc.

Active Publication Date: 2020-07-03
SHAANXI BUCHANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above-mentioned detection method has many steps in operation cost, which is obviously far from enough to control the internal quality of traditional Chinese medicine preparations.

Method used

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  • Substance content determination method and application thereof
  • Substance content determination method and application thereof
  • Substance content determination method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1 drug hirudin content detection method of the present invention

[0043] 1. Instruments and reagents

[0044] Analytical balance (Mettler, Switzerland), HW.SY11-KP2 intelligent constant temperature water bath (Beijing Changfeng Instrument Co., Ltd.), 3-18KS centrifuge (Germany Sigma), KQ-500E ultrasonic cleaning machine (Kunshan Ultrasonic Instrument Co., Ltd.), Longshengzhi Capsules (Shaanxi Buchang Co., Ltd., batch number: 181005), leeches (provided by Buchang Co., Ltd.), normal saline (Shijiazhuang No. Erdi Biotechnology Co., Ltd.), tris, hydrochloric acid, thrombin T8020 (batch number: 417G062, Beijing Suolaibao Technology Co., Ltd.)

[0045] 2. Methods and Results

[0046] 2.1 Solution preparation

[0047] 2.1.1 Preparation of the test solution

[0048] Take 0.4g, 0.4g, 0.4g, 0.4g, 0.4g, 0.4g, 0.4g, 0.3g, 0.2g, 0.1g respectively, add 2mL, 4mL, 4.44mL, 5mL, 5.7mL, 6.67 mL, 7.5mL, 6.67mL, 5mL of physiological saline, made with a concentration of 0.2g...

Embodiment 2

[0103] Embodiment 2 medicine is prepared as capsule

[0104] Prescription: 225g astragalus, 100g leech, 90g Chuanxiong, 90g angelica, 90g safflower, 113g peach kernel, 90g red peony, 90g woody, 90g calamus, 60g earthworm, 90g mulberry, 35g Acanthopanax extract;

[0105] Preparation method: take leeches, earthworms, and Acanthopanax dry extracts and grind them into fine powder; add water to decoct twice, and boil for the first time The second time is 2 hours, the second time is 1.5 hours, filter, combine the decoction, the relative density of the filtrate is 1.1-1.2 when the filtrate is concentrated to 60 ° C, spray dry into powder, add earthworm, leech, Acanthopanax powder and mix evenly, add medicine With starch, make 1000 grains, that is to say.

[0106] Usage and Dosage: Oral. 3 to 5 capsules at a time, 3 times a day.

Embodiment 3

[0107] Embodiment 3 medicine is prepared as tablet

[0108] Prescription: 225g astragalus, 100g leech, 90g Chuanxiong, 90g angelica, 90g safflower, 113g peach kernel, 90g red peony, 90g woody, 90g calamus, 60g earthworm, 90g mulberry, 35g Acanthopanax extract;

[0109] Preparation method: take leeches, earthworms, and Acanthopanax dry extracts and grind them into fine powder; add water to decoct twice, and boil for the first time The second time is 2 hours, the second time is 1.5 hours, filter, combine the decoction, the relative density of the filtrate is 1.1-1.2 when the filtrate is concentrated to 60°C, spray dry into powder, add earthworm, leech, Acanthopanax powder and mix evenly, add appropriate amount starch slurry, magnesium stearate, blended, granulated, and compressed into tablets.

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Abstract

The invention relates to a substance content determination method and an application thereof. The substance is prepared from 225 parts of astragalus membranaceus, 100 parts of leech, 90 parts of ligusticum wallichii, 90 parts of angelica sinensis, 90 parts of safflower carthamus, 113 parts of peach kernel, 90 parts of radix paeoniae rubra, 90 parts of elecampane, 90 parts of rhizoma acori graminei, 60 parts of lumbricus, 90 parts of parasitic loranthus and 35 parts of acanthopanax extract. The content detection method has characteristics of simple and rapid operation and good repeatability andaccuracy. The method is advantaged in that pharmacological experiments of the substance show that the substance can significantly improve the mouse behavior coordination ability, significantly shorten the mouse pole climbing time, enhance the mouse grabbing force and the mouse swimming time, and significantly relieve MPTP-induced neuron damage, the substance content determination method can effectively control the inherent quality of the drug, and the new treatment application of the substance enlarges the clinical medication disease treatment range of the drug.

Description

technical field [0001] The invention relates to a substance content determination method and an application thereof, belonging to the field of quality detection content methods. Background technique [0002] Containing multiple active ingredients hirudin and thrombin etc. in the contained drug leech of material of the present invention, it has effects such as anticoagulant, antithrombotic, fibrinolysis. Meng Xinyan, Yang Chengzhi, Research on the Correlation Between Plasma Antithrombin Level and Cerebral Infarction, Journal of Beihua University, 2018-11, It was found that the level of plasma antithrombin (AT-Ⅲ) was significantly decreased in the plasma of patients with cerebral infarction , and was highly correlated with the severity of cardiovascular and cerebrovascular diseases. Wu Yang, Yan Ge et al., Study on Quality Standards Evaluation of Antithrombin Activity of Compound Vipervein Capsules, Journal of Practical Medicine, 2019-01, literature report, using thrombin tit...

Claims

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Application Information

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IPC IPC(8): G01N31/16G01N30/90A61K36/888A61P9/00A61P9/10A61P25/16
CPCG01N31/16G01N30/90A61K36/481A61K35/62A61K36/236A61K36/232A61K36/286A61K36/736A61K36/71A61K36/65A61K36/285A61K36/888A61K36/185A61K36/254A61P25/16A61P9/10A61P9/00A61K2300/00
Inventor 王伟赵步长孙晓丽赵涛赵超王益民
Owner SHAANXI BUCHANG PHARMA
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