The invention discloses a preparation method of a Zhenshi stagnation eliminating decoction and application of the Zhenshi stagnation eliminating decoction in
pancreatic cancer treatment, and belongs to the technical field of traditional Chinese medicines. The preparation method comprises the following steps: weighing 6-12 parts by weight of
radix bupleuri, 8-15 parts by weight of
codonopsis pilosula, 10-15 parts by weight of fried
coix seed, 6-12 parts by weight of rhizoma bolbostemmae, 12-20 parts by weight of
turtle shell, 8-15 parts by weight of
pericarpium citri reticulatae, 6-12 parts by weight of fried immature bitter orange and 2-5 parts by weight of
coptis chinensis; the medicinal materials are decocted twice and then concentrated into extract with the
relative density of 1.10-1.25 to prepare a water decoction, and the water decoction can be further processed into granules or tablets. By up-regulating Bax
protein and down-regulating Bcl-2 and Bcl-xl
protein expression, the decoction induces
tumor cell apoptosis, inhibits mouse
pancreatic cancer transplantation tumor growth, maintains tumor-bearing mouse body
mass and prolongs swimming time, and the safety of the decoction is obviously superior to that of
chemotherapy drugs; according to the invention, the
traditional Chinese medicine theory and modern
pharmacology are combined, the anti-
pancreatic cancer traditional Chinese medicine compound with multiple targets and low toxic and side effects is provided, the compound has high efficiency,
repeatability and
dosage form diversity, and the blank of the
traditional Chinese medicine compound in accurate treatment of pancreatic
cancer is filled.