284 results about "Alkaline proteinase" patented technology

The invention discloses a preparation method and permeation promotion application of a high-purity sponge bone needle, and belongs to the technical field of sponge bone needles. Comprising the following steps: cutting a natural sponge into pieces, soaking in deionized water, carrying out ultrasonic oscillation, filtering, and cleaning to obtain a pretreated sponge; carrying out enzymolysis on the pretreated sponge with alkaline protease to obtain a primary zymolyte; and carrying out enzymolysis with ginger protease to obtain a secondary enzymolysis product, and carrying out vacuum freeze drying to obtain the high-purity sponge spicule. The natural sponge is subjected to enzymolysis treatment through alkaline protease and ginger protease, and the high-purity sponge spicule is obtained; wheat oligopeptide is grafted on the surface of a sponge spicule, and giant salamander skin collagen, a pomegranate seed extract, a lonicerae flos extract, a centella asiatica extract, an early celery extract, an iris tectorum extract, an oyster shell extract and calcium chloride mixed treatment materials are loaded, so that the repairing effect on skin inflammation acne is improved.

The invention discloses a preparation method and application of anti-photoaging collagen polypeptide. The preparation method comprises the following steps: unfreezing and cleaning silver carp skin, airing and cutting; naHCO3 is added, and soaking is carried out; adding Na2CO3 and carrying out ultrasonic impurity removal; performing ultrasonic-assisted treatment; adding protease for enzymolysis treatment; establishing an enzymolysis kinetic model under the alkaline protease condition; standing, cooling and centrifuging the enzymatic hydrolysate, collecting supernate, filtering, and performing vacuum freeze drying to obtain faint yellow SCSCP; the light aging resistant peptide SCSCP02 is separated, and the active peptide sequences of the light aging resistant peptide SCSCP02 are GPPGPPGTPGPQ, SGLPGPIGPPGPR and GLPGPIGPPGPR. The active substances which are extracted from the fish skin and have the anti-photoaging effect can reduce the risk of skin light damage caused by UVR by acting on various treatment targets, resource waste can be reduced, environmental pollution caused by biological resources can be relieved, and sustainable development of the industry can be promoted.

The invention relates to the technical field of biology, in particular to a DPP-IV inhibitory peptide extracted from buffalo cheese protein hydrolysate and application of the DPP-IV inhibitory peptide. An active component with the molecular weight smaller than 3 kDa is obtained through alkaline protease enzymolysis, ultrafiltration separation and LC-MS / MS identification, and the DPP-IV enzyme inhibitory activity IC50 value of the active component is 12.6 mg / mL. Four new DPP-IV inhibitory peptide fragments are found, are amino acid sequences in a sequence table SEQ ID NO: 1-4, have good DPP-IV inhibitory activity, and have good application prospects in being developed into functional foods or medicines for regulating blood sugar. In conclusion, the enzymolysis product and the peptide fragment can be prepared into antidiabetic drugs or functional foods, and have the advantages of being natural, safe and efficient. The invention provides a new strategy for high-valued utilization of buffalo milk resources and treatment of type 2 diabetes.

The invention provides a whey protein deep hydrolysate with low sensitization and an allergy relieving effect as well as a preparation method and application of the whey protein deep hydrolysate. According to the method, the alkaline protease, the neutral protease and the glutamyltransferase are sequentially used for carrying out first enzymolysis, second enzymolysis and third enzymolysis on the whey protein raw material, and the whey protein hydrolysate which is low in molecular weight, good in taste, good in digestion and absorption performance, low in sensitization and capable of effectively relieving allergic reaction of the organism is obtained.

The invention discloses a preparation method of a crocodile hydrolyzed protein zinc skin care product, and relates to the technical field of skin care. The method comprises the following steps: step a, pretreating raw materials: taking fresh crocodile meat and bones, cleaning, mechanically crushing until the particle size is less than 5mm, and adding water according to the ratio of 1: (3-5) to prepare homogenate; step b, performing three-step enzymolysis, namely performing enzymolysis by adopting alkaline protease, flavourzyme and zinc chelating enzyme in sequence; according to the constructed technology of three-step enzymolysis and three-stage membrane separation, accurate preparation of crocodile protein zinc is achieved, and the zinc chelating rate is increased to 90% or above through the gradient effect of alkaline protease, flavourzyme and zinc chelating enzyme and directional release of zinc binding sites and is remarkably higher than that of a traditional single-enzyme hydrolysis technology; meanwhile, a combined purification technology of a ceramic membrane, an ultrafiltration membrane and a nanofiltration membrane is adopted to replace chemical precipitation and organic solvent extraction, so that the completeness of active ingredients is reserved, the risk of environmental pollution is reduced, and the method conforms to the green manufacturing trend of food industry.

The invention discloses a preparation method for simultaneously and efficiently extracting crocodile polypeptide and crocodile oil, and relates to the technical field of biological extraction, crocodile meat is used as a raw material, and heating micro-pressure treatment is sequentially performed to promote component dissolution; a multi-stage enzymolysis process is adopted, alkaline protease, neutral protease and lipase are sequentially used, and a calcium phosphate system is used for assisting filtration and a natural antioxidant is added in a gradient manner in the enzymolysis process; performing plate frame filtration and standing layering subsequently to realize crude separation of the oil peptide; finally, the crocodile polypeptide liquid is subjected to multi-stage membrane filtration and spray drying, the crocodile crude oil is subjected to ultrasonic-assisted fishy smell removal, ceramic membrane fine filtration and gradient cooling phase change refining, and after enzymolysis treatment and before filtration, a composite additive is added into enzymatic hydrolysate and stirred. No organic solvent exists in the whole process, and the product yield, purity and oxidation stability are remarkably improved.

The invention discloses a corn peptide for dispelling effects of alcohol and protecting liver as well as a preparation method and application thereof, and belongs to the technical field of biological peptide extraction. The preparation method comprises the following steps: crushing and sieving corn, dissolving the corn in deionized water, adjusting the pH value to be alkaline, and performing ultrasonic-assisted extraction to obtain corn homogenate; centrifuging, taking supernate, adjusting the pH to be neutral, centrifuging, adding an ethanol solution with the mass fraction of 10%, standing, carrying out continuous flow centrifugation, suspending precipitates in distilled water, dialyzing in a dialysis bag, and freeze-drying to obtain crude corn protein; the preparation method comprises the following steps: preparing an aqueous solution, carrying out variable-temperature treatment, adjusting the pH value to be alkaline, adding trypsin and alkaline protease, carrying out enzymolysis, repeating the enzymolysis twice, and after the enzymolysis is finished, carrying out heating enzyme deactivation, centrifugation, sediment discarding, ultrafiltration, centrifugation, supernatant taking and spray drying to obtain the corn peptide. The corn peptide can remarkably shorten the drunkenness time and sobering time of mice and reduce the drunkenness duration, and has good effects of dispelling the effects of alcohol and protecting the liver.

The present invention discloses a black chicken liver hydrolyzed peptide that can be used to improve ulcerative colitis, and its preparation method and application, which belong to the field of biomedicine technology. The preparation method of the black chicken liver hydrolyzed peptide comprises the following steps: making black chicken liver into minced meat and mixing it with water, heating the obtained liquid material, adding alkaline protease for enzymatic hydrolysis, inactivating the enzyme and collecting the supernatant by centrifugation, and freeze-drying to obtain the black chicken liver hydrolyzed peptide. The present invention adopts formaldehyde titration to detect the degree of hydrolysis, adopts SDS-PAGE electrophoresis and Tricine-SDS-PAGE electrophoresis to detect the molecular weight, performs sequence analysis by LC-MS / MS, uses a database to predict the biological function of peptide segments, detects the antioxidant and blood pressure lowering ability of black chicken liver peptide by in vitro experiments, and detects the effect of black chicken liver peptide in improving colitis by mouse experiments. The results show that the black chicken liver peptide prepared by the present invention has the advantages of antioxidant, blood pressure lowering and improvement of ulcerative colitis.

The invention aims to provide a chlorella pyrenoidosa peptide with an effect of preventing alcoholic liver injury, which is prepared by the following steps: crushing chlorella pyrenoidosa, mixing with water, freezing and thawing, and performing ultrasonic treatment to prepare homogenate; alkaline protease is added for enzymolysis, and then flavourzyme is added for enzymolysis; and after the enzymolysis product is adsorbed by activated carbon, the supernate is adsorbed by using an SP Sephadex C25 cation exchange medium. The amino acid sequence of the chlorella pyrenoidosa peptide is PSEQ ID NO: 1-5. The chlorella pyrenoidosa peptide provided by the invention is obtained by enzymolysis, adsorption and cation exchange chromatography technologies, and is verified by a HepG2 cell model to obviously relieve alcoholic liver injury. The raw materials are natural and safe, the process is accurate and efficient, and industrialization potential is achieved. Meanwhile, the peptide is suitable for development of functional food, health care products and medicines, and a low-toxicity and sustainable liver protection solution is provided.

The invention provides earthworm protein peptide as well as a preparation method and application thereof, and belongs to the technical field of medicines. The preparation method comprises the following steps: drying and crushing earthworms to obtain earthworm powder, adding the earthworm powder into water, adding immobilized compound enzyme for enzymolysis, carrying out magnet separation, adjusting the pH value of enzymatic hydrolysate for precipitation, collecting solids, adding the solids into water, adding a purifying agent for adsorption separation, adding the solids into a deep eutectic solvent, heating for desorption, carrying out magnet separation, carrying out ultrafiltration on residual liquid, and collecting protein peptide to obtain the earthworm protein peptide. The immobilized compound enzyme is prepared by immobilizing modified snail protease and alkaline protease on chitosan modified MOF / magnetic microspheres; the purifying agent is a passivated angiotensin converting enzyme immobilized magnetic agar ball. The high-activity earthworm protein peptide has the advantages of simple preparation method, high purity, good inhibition effect on angiotensin converting enzyme, safety, high efficiency, no side effect, good antihypertensive effect on hypertensive patients, good patient compliance, and wide application prospect.

The invention discloses an enzymolysis preparation process of wood frog skin micromolecule III type collagen peptide. The enzymolysis preparation process comprises the following steps: mixing defatted wood frog skin powder with water, and pretreating; adding a papain-neutral protease-glutamine protease hydrolysis system into the pretreated mixed solution, continuously hydrolyzing, performing centrifugal treatment after the hydrolysis is completed, heating supernate, adding alkaline protease into the supernate for hydrolysis and enzyme inactivation, and performing centrifugal treatment to obtain clear liquid rich in collagen peptide; carrying out multistage membrane separation coupling chromatography purification treatment and low-temperature vacuum concentration treatment on the clear liquid rich in collagen peptide to obtain a concentrated solution; and mixing the concentrated solution with a trehalose-hyaluronic acid composite wall material, and performing spray drying to obtain the collagen peptide nanocapsule. Efficient preparation and function enhancement of the wood frog skin type III collagen peptide can be achieved, and the prepared collagen peptide has the purity larger than or equal to 99.5% and the activity retention rate larger than 98% and is excellent in heat stability and moisturizing performance.

The invention provides a nutrition enhancer beneficial to bone health of middle-aged and elderly people, a preparation method of the nutrition enhancer and application of the nutrition enhancer in preparation of medial care food, and belongs to the field of nutritious food. The nutrient supplement comprises a plant fermentation product, osteopontin peptide, collagen peptide, N-acetylglucosamine and calcium, vitamins and mineral substances can be added, and the plant fermentation product is obtained by performing anaerobic fermentation on rhizoma drynariae and chestnuts through a complex microbial inoculant composed of lactobacillus reuteri and bifidobacterium longum subsp. Longum; the collagen peptide is prepared by performing enzymolysis on bovine bones through alkaline protease and flavourzyme; the hydrogel is prepared by adopting a sodium alginate-calcium chloride-carboxymethyl chitosan micro-capsule embedding process. An in-vitro experiment shows that the nutrition enhancer can remarkably promote osteoblast proliferation, differentiation and mineralization; animal experiments show that the bone mineral density of osteoporosis model mice can be effectively improved. The nutrition enhancer disclosed by the invention is suitable for preparing care food and bone health products, and has a remarkable effect of improving bone health of middle-aged and elderly people.

The invention discloses a method for preparing high-F-value oligopeptide from whey protein, and belongs to the technical field of food biology. According to the method, whey protein is taken as a raw material, step-by-step synergistic enzymolysis of alkaline protease-chymotrypsin-flavourzyme is performed, and an activated carbon selective adsorption dearomatization process is combined, so that the F value and the hydrolysis degree of oligopeptide are remarkably improved. The enzymolysis efficiency is high, and the final hydrolysis degree is gt; 30%; the dearomatization effect is excellent, and the product F value is gt; 26; the method is simple in process and low in cost, high-value conversion of whey protein can be achieved, and the prepared high-F-value oligopeptide can be applied to the fields of sports nourishment, food for special medical purposes and the like and has remarkable industrial value.

The invention discloses an egg yolk polypeptide capable of improving learning cognitive ability and a preparation method and application thereof, and the egg yolk polypeptide capable of improving learning cognitive ability is obtained by combining ultrasonic alkali treatment with alkaline protease enzymolysis and desalting by using an integrated membrane separation technology. According to the preparation method, the compact structure of the yolk can be destroyed through the ultrasonic alkali treatment and the enzymolysis process, the enzymolysis efficiency of the yolk can be improved, a large number of bioactive peptides are released, the bioactive peptide yield of a final product is stabilized to be 60% or above, the molecular weight of the bioactive peptides is intensively distributed to be 180-2000 Da, and the nutrition utilization rate is improved. The egg yolk polypeptide prepared by the method can significantly increase the value-added rate of nerve cells and improve the spatial memory ability and cognitive flexibility of mice, ultrasonic alkali treatment is combined with biological enzymolysis, no organic solvent or strong acid and strong alkali is added, and the production process of natural protein hydrolysate is natural and safe and meets the food-grade safety production standard.

The invention relates to a method for reducing the anaphylaxis of natural latex by multi-enzyme cascade catalysis of rubber protein. The invention relates to the technical field of catalysis of rubber protein hydrolysis, in particular to a method for reducing the anaphylaxis of natural latex by thoroughly hydrolyzing rubber protein through multi-enzyme cascade catalysis. The method for reducing the anaphylaxis of the natural latex by catalyzing the rubber protein through the synergistic effect of multiple enzymes from the perspective of industrial production comprises the following steps: adding an enzyme A and an enzyme B into the natural latex for reaction so as to reduce the anaphylaxis of the natural latex, the enzyme A is alkaline protease; and the B enzyme is at least one of aminopeptidase, carboxypeptidase B, carboxypeptidase A and carboxypeptidase Y. According to the method disclosed by the invention, the enzyme A-endopeptidase and the enzyme B-exopeptidase are subjected to multi-enzyme-stage combined treatment and concerted catalysis, so that the enzyme A-endopeptidase and the enzyme B-exopeptidase are completely hydrolyzed or are completely hydrolyzed into more amino acid substances and short-chain peptides (oligopeptides) as far as possible, and the amino acid substances and the short-chain peptides (oligopeptides) are retained in latex. Protein is highly hydrolyzed into amino acid with higher safety, so that the possibility of I-type anaphylactic reaction caused by protein allergens is greatly reduced or even eliminated.

The invention discloses camellia seed meal oligopeptide as well as a preparation method and application thereof. The method comprises the following steps: S1, pretreating camellia seed meal to obtain a pretreated substance; s2, performing enzymolysis and wall breaking to obtain first enzymatic hydrolysate; wherein the enzymolysis wall breaking adopts a mixed enzyme, and the mixed enzyme comprises amylase and cellulase; s3, extracting crude protein to obtain crude protein; s4, carrying out crude protein enzymolysis on the crude protein to obtain a second enzymatic hydrolysate; wherein the crude proteolysis adopts mixed protease, and the mixed protease comprises first protease and second protease; wherein the first protease is one or more of alkaline protease, neutral protease and acid protease; the second protease is papain; and S5, purifying the second enzymatic hydrolysate to obtain the camellia seed meal oligopeptide. The average molecular weight of the obtained product is smaller than 1000 Daltons, and the product has biological activity and high application value in the fields of cosmetics and functional food.

The invention belongs to the technical field of microbial protein separation and extraction, and particularly relates to soluble microbial protein and a preparation method thereof. The method comprises the following steps: fermenting and culturing microbial thalli to a logarithmic phase, and centrifuging to obtain mycelia; the mycelium is freeze-dried and then smashed, and mycelium powder is obtained; the mycelium powder is placed in water to be dispersed and homogenized, and homogenized homogenate is obtained; finally, alkaline protease is added into the homogenate for enzymolysis and enzyme deactivation, and the soluble microbial protein is obtained. On one hand, microbial cell walls are directly destroyed through mechanical shearing force and collision action generated by high-speed rotation of the wall breaking machine, and intracellular proteins and active ingredients are released; on the other hand, high-pressure homogenization is combined, mechanical shear force and cavitation effect are utilized to destroy a protein compact structure and expose restriction enzyme cutting sites, and alkaline protease is combined for directional hydrolysis, so that the enzymolysis time is greatly shortened, the efficiency is improved, and the extraction rate is greatly improved.

The invention belongs to the field of biological material extraction, and particularly relates to a method for extracting collagen peptide from marine organisms, which comprises the following steps: S1, crushing marine organism raw materials, soaking the crushed marine organism raw materials in a citric acid buffer solution containing alginate oligosaccharide for swelling treatment, and then performing high-voltage pulse electric field treatment to obtain pretreated collagen slurry; and S2, carrying out first-stage enzymolysis treatment on the pretreated collagen slurry through alkaline protease, and then carrying out second-stage enzymolysis treatment through collagenase and flavourzyme. S3, performing separation treatment through a first ultrafiltration membrane to obtain first filtrate, performing deodorization treatment on the first filtrate through a first nanofiltration membrane of which the surface is coated with a beta-cyclodextrin adsorbent to obtain second filtrate, and performing separation treatment on the second filtrate through a second nanofiltration membrane to obtain third filtrate. And S4, concentrating, desalting and drying the third filtrate to obtain collagen peptide powder. Through the synergistic effect of multiple steps, the concentration of the molecular weight of the collagen peptide and the efficient deodorization are realized on the basis of retaining the active structure of the collagen.

The invention discloses a hippophae rhamnoides seed meal protein-based hypoglycemic peptide and a preparation method thereof, and the preparation method comprises the following steps: mixing hippophae rhamnoides seed meal powder with water, adjusting the pH value with sodium hydroxide, stirring, and centrifuging to obtain a supernatant; adjusting the pH value of the supernatant with hydrochloric acid, standing, centrifuging, washing the obtained precipitate with distilled water until the precipitate is neutral, pre-freeze-drying, and freeze-drying to obtain SSP powder; the preparation method comprises the following steps: mixing SSP powder with water to obtain an SSP solution, carrying out water bath preheating, adding protease, carrying out enzymolysis, carrying out water bath heating on the solution, adjusting the pH value of the solution to be neutral, collecting a centrifugal supernatant, and carrying out low-temperature dialysis to obtain the hypoglycemic peptide based on the sea buckthorn seed meal protein, wherein the protease comprises alkaline protease, compound protease, neutral protease, bromelain and papain, and the invention aims to excavate the development prospect of the sea-buckthorn seed meal active peptide and expand the application potential of the sea-buckthorn seed meal active peptide in different disease fields.

The invention relates to the field of polypeptides, in particular to a black ginseng polypeptide and a preparation method and application thereof. The black ginseng polypeptide is obtained by taking black ginseng as a raw material through high-pressure homogenization wall breaking and alkaline protease enzymolysis. The polypeptide has an anti-aging function. The black ginseng polypeptide provided by the invention is more efficient, safer and more environment-friendly, and has a good application prospect in the fields of cosmetics, functional foods and biological medicines.

The application discloses a method for preparing oil-tea camellia seed oil by alkaline protease and endogenous protease synergistic demulsification, which comprises the following steps: 1) collecting emulsion; 2) extracting endogenous protease; 3) alkaline protease and endogenous protease synergistic demulsification: adding distilled water and alkaline protease into the emulsion for primary enzymolysis, the adding amount of the alkaline protease is 0.5-2% of the weight of the emulsion, the primary enzymolysis time is T1, and 60min<=T1<75min, adding endogenous protease according to 0.6-1.4% of the weight of the emulsion for secondary enzymolysis, the secondary enzymolysis time is T2, and 15min<=T2<=35min, the total time of the primary enzymolysis and the secondary enzymolysis is less than 100min, and oil emulsion is obtained; 4) enzyme inactivation and centrifugation, and oil-tea camellia seed oil is obtained. The alkaline protease and the endogenous protease are used for synergistic demulsification, which can not only improve the demulsification rate and the extraction rate of the oil-tea camellia seed oil, but also inhibit the generation of bitter amino acids, so that the prepared oil-tea camellia seed oil does not contain bitterness, and the flavor and quality of the oil-tea camellia seed oil are improved.

The invention discloses a preparation method of a red rice bran protein peptide compound with antioxidant and hypoglycemic effects. According to the method, red rice bran protein is extracted through an alkali-solution and acid-isolation method; hydrolyzing the red rice bran protein with alkaline protease to obtain a red rice bran protein hydrolysate; the red rice bran protein hydrolysate prepared by the invention has the functional characteristics of excellent emulsibility, foamability and oil absorbency, and has excellent antioxidant and hypoglycemic functional activities after being subjected to in-vitro simulated gastrointestinal digestion. The red rice bran protein hydrolysate can be used in plant-based food and dairy product substitutes and functional food with a hypoglycemic effect.

The present application relates to the technical field of small molecule peptide preparation, and specifically discloses a preparation process of selenium-rich antioxidant pig lung polypeptide. The preparation process of the selenium-rich antioxidant pig lung polypeptide comprises the following steps: washing and decontaminating fresh pig lung, peeling off the trachea, removing grease, and twisting into a minced meat shape to obtain pig lung pre-preparation material; mixing the pig lung pre-preparation material with a vitamin C aqueous solution, adding nano-silicon dioxide powder and eugenol to the mixture, and performing ultrasonic treatment; cooling to-10 to-30 DEG C, keeping for 1 to 2 hours, and then increasing the temperature to room temperature; homogenizing treatment, filtration, collecting the precipitate, and obtaining pretreated pig lung; adding water to the pretreated pig lung and stirring until uniform; adjusting the pH value of the system; adding a stabilizing alkaline protease for treatment; adjusting the system to be neutral; adding a neutral protease for enzymolysis; and cooling to room temperature to obtain pig lung enzymolysis liquid; centrifuging the pig lung enzymolysis liquid, ultrafiltering, collecting the permeate, nanofiltrating, collecting the concentrated liquid, and drying.

The application provides a mung bean protein salty taste enhancing peptide, a screening and preparation method and application. The method comprises the following steps: step 1, carrying out step-by-step enzymolysis on mung bean protein by using alkaline protease and flavor protease, carrying out ultrafiltration grading, and screening an initial extract of the salty taste enhancing peptide by using an electronic tongue; step 2, further identifying polypeptide sequences by using LC-MS / MS; and step 3, screening polypeptides with strong interaction capability with salty taste receptors TMC4 and ENaC by combining biological activity prediction, target active fragment screening, safety and physicochemical property evaluation and molecular docking analysis. The salty taste enhancing peptide of the mung bean protein obtained by screening comprises polypeptides represented by RPFF, APDLRGY, WDDIGGL, FDGF and AEFF, and contains RP, RG, DD, DGF and FF characteristic peptide segments, respectively. The polypeptides are synthesized by using a solid phase, and the salty taste enhancing effect of the polypeptides is verified by using an electronic tongue. When 0.03% of the above synthesized polypeptides are added to a 0.4% NaCl solution, the salty taste enhancing rates are 90%, 62%, 34%, 52% and 42%, respectively. The polypeptides can significantly enhance salty taste perception of a solution under low-salt conditions, and achieve the effect of salt reduction without taste reduction.

The invention discloses a composition for improving adverse effects of chemotherapy as well as a preparation method, application and a product of the composition, and particularly relates to the technical field of medicines. The composition comprises the following raw materials: tortoise, soft-shelled turtle, antler, sea cucumber, fructus amomi, pericarpium citri reticulatae and hawthorn. Alkaline protease and flavourzyme are adopted for enzymolysis, and the obtained composition can remarkably reduce the tumor volume of tumor-bearing animals, increase the bone marrow DNA content of chemotherapy animals and remarkably increase the number of white blood cells and the number of red blood cells in peripheral blood of the chemotherapy animals. The composition disclosed by the invention is used for preparing related products for improving the adverse effects of chemotherapy, and the prepared products are easy to absorb and have a remarkable improvement effect on the adverse effects of chemotherapy.

The present disclosure belongs relates to a 2709 alkali protease mutant modified based on molecular dynamics calculation and use thereof in liquid detergents. The dynamic structure feature of a 2709 alkali protease derived from Bacillus licheniformis are studied by using a molecular dynamics method through computer simulation, which is a unique effective method capable of studying a relationship between the structure of the 2709 alkali protease and molecular dynamics as well as an enzyme function in an atomic level. After molecular dynamics calculation, appropriate sites are selected for protein engineering to obtain the 2709 alkali protease mutant suitable for industrial application of detergents. The protease mutant has significantly improved enzyme activity under the alkaline condition, is better in stability, not only has good decontamination effect but also has good component compatibility with components in the detergent, and can be widely applied to the field of washing industry.

The invention relates to a novel amino acid foliar fertilizer and a preparation method thereof, and belongs to the technical field of agricultural fertilizers. The method aims at solving the technical problems that in the prior art, the amino acid conversion rate is low, product stability is poor, and severe conditions such as strong acid and strong alkali are possibly used. The method comprises the following steps: mixing soybean meal, fish meal and saccharomyces cerevisiae thalli to form a compound protein raw material, performing two-stage crushing and degreasing, performing step-by-step enzymolysis by adopting a compound enzyme preparation compounded by alkaline protease and flavourzyme, performing enzyme deactivation, and performing ultrafiltration by adopting a ceramic membrane to obtain purified amino acid hydrolysate, and mixing the hydrolysate with a composite trace element mixed solution formed by EDTA-Fe, EDTA-Zn, boric acid, ammonium molybdate and citric acid, adding humic acid, a seaweed extract and an organic silicon penetrant, and carrying out high-speed shearing, filtering and vacuum degassing to finally obtain the product. The method is mainly used for producing the amino acid foliar fertilizer which is high in free amino acid content, good in stability and easy to absorb by plants.

The invention belongs to the technical field of protein, and relates to an anti-photoaging damage peptide and a preparation method thereof. The amino acid sequence of the peptide is KSYELPDGQVITI, and the key active fragment of the peptide is ELPDGQVIT. The peptide is obtained by performing enzymolysis on degreased maggot powder with alkaline protease, trypsin, papain, neutral protease and flavourzyme. The peptide can remove ROS free radicals after UVA irradiation, improve SOD activity and inhibit MDA generation, and finally alleviate oxidative stress caused by UVA.

The invention discloses a high-emulsification-activity gluten protein based on irradiation modification and a preparation method thereof in the field of wheat protein processing, and the method comprises the following steps: dispersing wheat gluten protein in deionized water, stirring to form a uniform suspension, adjusting the pH value, carrying out enzymolysis treatment on the suspension by using alkaline protease, and drying to obtain the high-emulsification-activity gluten protein. Centrifugally drying to obtain primary protein powder; dissolving the protein primary powder in an irradiation solvent to obtain a protein dispersion liquid, and irradiating the protein dispersion liquid by using cobalt-60 gamma rays as an irradiation source in an intermittent manner; and taking the irradiated protein dispersion liquid, and performing centrifugal drying to obtain the high-emulsification active gluten protein. The method comprises the following steps: firstly, carrying out limited hydrolysis on gluten protein by using protease to generate a smaller peptide fragment, so that the irradiation efficiency and the emulsion stability are improved; by adopting an intermittent irradiation treatment mode, protein aggregation caused by heat effect can be reduced, the emulsifying effect is improved, and the stability and adhesive force of an adhesive can be improved when the emulsion is applied to the adhesive.