Preparation method of cordyceps militaris polypeptide

A technology of Cordyceps militaris and slurry, which is applied in the field of bioengineering, can solve the problems of few polypeptide research methods and the like, and achieve the effects of realizing automatic control, simple operation and easy automatic control.

Inactive Publication Date: 2013-07-10
赤峰鼎基生物工程科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are few methods for preparing polypeptides from fresh Cordyceps

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: a kind of preparation method of Cordyceps militaris polypeptide comprises the following steps:

[0023] (a) removing impurities, cleaning, pulverizing and grinding fresh Cordyceps militaris to obtain Cordyceps militaris abrasive liquid;

[0024] (b) breaking the wall of the Cordyceps militaris grinding liquid by freezing and thawing the cells, freezing the Cordyceps militaris grinding liquid at -20° C. for 24 hours, and then placing it at 4° C. for 10 hours to obtain the broken wall Cordyceps militaris grinding liquid;

[0025] (c) adding the broken wall Cordyceps militaris grinding liquid to pure water for homogenization, and the weight ratio of the broken wall Cordyceps militaris grinding liquid to pure water is 1: 4 to obtain the Cordyceps militaris homogeneous slurry;

[0026] (d) adding a compound enzyme composed of fibrinase and pectinase to the homogenate of Cordyceps militaris, and enzymatically hydrolyzing it at a temperature of 36° C. for 5 hours...

Embodiment 2

[0033] Embodiment 2: a kind of preparation method of Cordyceps militaris polypeptide comprises the following steps:

[0034] (a) removing impurities, cleaning, pulverizing and grinding fresh Cordyceps militaris to obtain Cordyceps militaris abrasive liquid;

[0035] (b) breaking the wall of the Cordyceps militaris grinding liquid by freezing and thawing the cells, freezing the Cordyceps militaris grinding liquid at -20° C. for 24 hours, and then placing it at 4° C. for 10 hours to obtain the broken wall Cordyceps militaris grinding liquid;

[0036] (c) Adding the broken wall Cordyceps militaris grinding liquid to pure water for homogenization, the weight ratio of the broken wall Cordyceps militaris grinding liquid to the purified water is 1:5 to obtain the Cordyceps militaris homogeneous slurry;

[0037] (d) adding a compound enzyme composed of fibrinase and pectinase to the homogenate of Cordyceps militaris, and enzymatically hydrolyzing it for 4.5 hours at a temperature of 3...

Embodiment 3

[0044] Embodiment 3: a kind of preparation method of Cordyceps militaris polypeptide comprises the following steps:

[0045] (a) removing impurities, cleaning, pulverizing and grinding fresh Cordyceps militaris to obtain Cordyceps militaris abrasive liquid;

[0046] (b) breaking the wall of the Cordyceps militaris grinding liquid by freezing and thawing the cells, freezing the Cordyceps militaris grinding liquid at -20° C. for 24 hours, and then placing it at 4° C. for 10 hours to obtain the broken wall Cordyceps militaris grinding liquid;

[0047] (c) Adding the broken wall Cordyceps militaris grinding liquid to pure water for homogenization, the weight ratio of the broken wall Cordyceps militaris grinding liquid to pure water is 1: 6 to obtain the Cordyceps militaris homogeneous slurry;

[0048] (d) adding a compound enzyme composed of fibrinase and pectinase to the homogenate of Cordyceps militaris, and enzymatically hydrolyzing it for 4 hours at a temperature of 40° C.;

...

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PUM

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Abstract

The invention discloses a preparation method of cordyceps militaris polypeptide. The preparation method comprises the steps of: based on fresh cordyceps militaris as a raw material, preparing cordyceps militaris homogenate liquid by crushing, grinding, freeze thawing and homogenizing; carrying out enzymolysis of compound enzymes consisting of plasmase and pectinase, the enzymolysis of papain, the enzymolysis of alkaline proteinase and the enzymolysis of flavourzyme on the cordyceps militaris homogenate liquid; and finally carrying out enzyme inactivation, filtering by utilizing a molecular film filter and spray-drying on the cordyceps militaris homogenate liquid, so as to obtain cordyceps militaris polypeptide powder. The preparation method can be carried out at normal temperatures and pressures and has the advantages of simple operation method, energy conservation and environmental friendliness, low cost, high extraction efficiency of the cordyceps militaris polypeptide and high polypeptide contents of products.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a method for preparing a polypeptide of Cordyceps militaris. technical background [0002] The cultivation process of Cordyceps militaris is the result of biomimetic cultivation under the growth conditions of wild Cordyceps sinensis. It is rich in three major nutrients of protein, fat and sugar, and has high nutritional value. It has a wide range of uses and is suitable for beverages, alcohol, health care products, etc. It has a large market demand, broad prospects and high product value. At present, there are many researches on Cordyceps militaris at home and abroad, and the development and utilization of Cordyceps militaris are mostly based on extracting active substances such as cordycepin, cordyceps polysaccharide, and cordycepic acid. There are few methods for preparing polypeptides from fresh Cordyceps. How to better develop and utilize the new resource food of Cor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34
Inventor 刘永祥
Owner 赤峰鼎基生物工程科技有限公司
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