Miichthys miiuy air bladder antioxidation peptide and application thereof

A technology of antioxidant peptides and swim bladders, applied in the fields of peptides, antidote, food science, etc., can solve the problems of increasing the difficulty of extracting antioxidant peptides, low purity of antioxidant peptides, etc., and achieve good lipid antioxidant capacity and enzymatic hydrolysis process. Easy-to-control, vitality-boosting effect

Inactive Publication Date: 2018-01-16
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this preparation method does not have a degreasing step, which increases the difficulty of extracting antioxidant peptides. In addition, the purity of the obtained antioxidant peptides is relatively low.

Method used

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  • Miichthys miiuy air bladder antioxidation peptide and application thereof
  • Miichthys miiuy air bladder antioxidation peptide and application thereof
  • Miichthys miiuy air bladder antioxidation peptide and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0040] A squid swim bladder antioxidant peptide, the preparation method of which specifically comprises the following steps:

[0041] 1) Pretreatment of squid swim bladder: After the squid (Miichthys miiuy) swim bladder is washed and homogenized to a paste, add 0.08M NaOH solution at a solid-to-liquid ratio of 1g:15mL, soak at 5°C for 12h, filter, and wash with distilled water Wash until neutral, add 10% isopropanol solution according to the solid-to-liquid ratio of 1g:8mL, degrease for 36 hours, filter, discard the filtrate, rinse the solid matter with distilled water with isopropanol, and dry to obtain defatted squid swim bladder;

[0042] 2) Enzymatic hydrolysis of squid swim bladder: Take the defatted squid swim bladder, add distilled water according to the ratio of solid to liquid 1g:8mL, adjust the pH value to 9, add 2% alkaline protease of the swim bladder weight, enzymolyze at 60°C for 4h, and then in Inactivate the enzyme at 90°C for 15 minutes, cool to room temperatu...

Embodiment 2

[0050] A squid swim bladder antioxidant peptide, the preparation method of which specifically comprises the following steps:

[0051] 1) Pretreatment of squid swim bladder: after the squid (Miichthys miiuy) swim bladder is cleaned and homogenized to a paste, add 0.1M NaOH at 1 g: 10 mL and soak at 4°C for 15 hours, filter, wash with distilled water until neutral, and Add 10% isopropanol solution to 1g:5mL, degrease for 48 hours, filter, discard the supernatant, rinse the isopropanol with distilled water, and dry to obtain defatted squid swim bladder;

[0052] 2) Enzymatic hydrolysis of squid swim bladder: take the defatted squid swim bladder and add distilled water according to the ratio of material to liquid: 1g:5mL. Adjust the pH value to 9-10, add 2.5% alkaline protease (enzyme activity ≥ 2.0×105U / g) of swim bladder weight, enzymolyze at 55°C for 3.5h, inactivate the enzyme at 95°C for 10min, cool to room temperature, centrifuge at 12000rmp for 20min, Collect the supernata...

Embodiment 3

[0062] A squid swim bladder antioxidant peptide, the preparation method of which specifically comprises the following steps:

[0063]1) Pretreatment of squid swim bladder: after the squid (Miichthys miiuy) swim bladder is cleaned and homogenized to a paste, add 0.1M NaOH at 1 g: 10 mL and soak at 4°C for 15 hours, filter, wash with distilled water until neutral, and Add 10% isopropanol solution to 1g:5mL, then add 0.022% and 0.004% sodium dodecylbenzenesulfonate and carvacrol by swim bladder weight, degrease for 48h, filter, discard the filtrate, and dissolve the solid matter with isopropanol with distilled water Rinse well and dry to obtain defatted squid swim bladder. The above-mentioned sodium dodecylbenzenesulfonate and carvacrol can play a synergistic effect with isopropanol to destroy the fat cell membrane, causing the fat in the cell to leak out, and at the same time increase the isopropyl alcohol. The dissolving ability of propanol can promote the rapid dissolution of ...

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Abstract

The invention discloses a Miichthys miiuy air bladder antioxidation peptide and application thereof. The antioxidation peptide Phe-Pro-Tyr-Leu-Arg-His (FPYLRH) is obtained through subjecting Miichthysmiiuy air bladders, which serve as a raw material, to defatting and alkaline proteinase enzymolysis to obtain an enzymolysis solution and subjecting the enzymolysis solution to ultrafiltration, anionexchange resin chromatography, gel column chromatography and out-phase high performance liquid chromatography separation and purification; proven by ESI-MS assaying, the antioxidation peptide has themolecular weight of 831.98Da. The antioxidation peptide has the beneficial effects that the prepared high-activity antioxidation peptide has a good scavenging action on DPPH free radicals and hydroxyl free radicals; meanwhile, the antioxidation peptide has a protective action on hydrogen peroxide induced human umbilical vein endothelial cell-HUVEC cellular oxidation injury and can serve as drugs,healthcare food or food additives.

Description

technical field [0001] The present invention relates to an antioxidant peptide, in particular to an antioxidant peptide of squid swim bladder and application thereof. Background technique [0002] Antioxidants are a class of substances that can help capture and neutralize free radicals to eliminate the damage caused by free radicals to the human body. They are food additives that can prevent or delay food oxidation, improve food stability and extend storage life. At present, chemically synthesized antioxidants, such as butyl hydroxyanisole, dibutyl hydroxytoluene, propyl gallate, tert-butyl hydroquinone, etc., are widely used in the food industry. However, chemically synthesized antioxidants damage the liver, kidneys and other organs of the human body to varying degrees, and some countries and regions have clearly limited or banned their use. Some natural antioxidants are also restricted by raw materials, prices and other factors, so the development of highly efficient and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C12P21/06C07K1/36C07K1/34C07K1/20C07K1/18A61K38/08A61P39/06A23L33/18
Inventor 王斌杨绣荣潘欣赵玉勤
Owner ZHEJIANG OCEAN UNIV
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