Method of preparing chondroitin sulfate

A technology of chondroitin sulfate and cartilage, which is applied in the field of directly preparing chondroitin sulfate from animal cartilage, can solve the problems of large amount of saline and alkali, long extraction time, insufficient extraction, etc., and achieve simplified preparation process, easy industrial production, The effect of shortening the production time

Inactive Publication Date: 2007-08-08
NANCHANG WANHUA BIOCHEM PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, during alkali treatment, the sulfhydryl group on the chondroitin sulfate can be detached, causing the degradation of chondroitin sulfate, reducing the yield of chondroitin sulfate, and the cost is higher
The disclosed patent "chondroitin sulfate and its extraction method" (patent application number: 96116057.8) adopts the dilute salt dilute alkali method, and its disadvantage is that the amount of saline alkali

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] (1) Put 10 kg of clean pig nose bones into the reactor, add about 40 L of water, add 0.08 kg of 2709 alkaline protease, adjust the pH to 8.5, control the temperature at about 48 ° C, and stir for 6 hours;

[0015] (2) After the reaction of cartilage fragments is complete, filter or centrifuge to remove slag, adjust the pH of the filtrate to 4.5 with hydrochloric acid, add 1.0 kg of activated carbon per 100 L of filtrate, stir for 30 minutes, filter or centrifuge to remove slag and decolorize;

[0016] (3) Add cetylpyridinium chloride 0.04kg to the clarified enzymolysis solution obtained in the upper step, so that the concentration of cetylpyridinium chloride in the solution reaches 0.1%, stir and stand for 3 hours, filter or centrifuge to obtain the precipitate ;

[0017] (4) Adding concentration to the precipitate in the previous step is 2% sodium acetate to dissociate, then add 95% ethanol that is 2.5 times the volume of the dissociated solution, so that the final con...

Embodiment 2

[0020] (1) Put 50 kg of clean pig nose bones into the reactor, add about 200 L of water, add 0.3 kg of 2709 alkaline protease, adjust the pH to 8.5, control the temperature at 45 ° C, and stir for 6 hours;

[0021] (2) After the reaction of cartilage fragments is complete, filter or centrifuge to remove slag, adjust the pH of the filtrate to 4.5 with acetic acid, add 0.2 kg of activated carbon per 100 L of filtrate, stir for 20 minutes, filter or centrifuge to remove slag and decolorize;

[0022] (3) Add 0.2 kg of cetylpyridinium chloride to the clarified enzymolysis solution obtained in the upward step, so that the concentration of cetylpyridinium chloride in the solution reaches 0.1%, stir and stand for 3 hours, filter or centrifuge to obtain the precipitate ;

[0023] (4) adding concentration to the precipitate in the previous step is 1% sodium chloride to dissociate, then add 95% ethanol 2 times the volume of the dissociated solution, so that the final concentration of eth...

Embodiment 3

[0026] (1) Put 120kg of clean pig nose bones into the reactor, add about 480L of water, add 1.8kg of 2709 alkaline protease, adjust the pH to 8.5, control the temperature at about 55°C, and stir for 6 hours;

[0027] (2) After the reaction of cartilage fragments is complete, filter or centrifuge to remove slag, the filtrate is adjusted to pH 4.5 with hydrochloric acid, add 2.0 kg of activated carbon per 100 L of filtrate, stir for 60 minutes, filter or centrifuge to remove slag, decolorize;

[0028] (3) add cetylpyridinium chloride 0.24kg in the clarification enzymolysis solution that step up obtains, make cetylpyridinium chloride concentration reach 0.05% in the solution, stir and leave standstill for 3 hours, filter or centrifuge to obtain precipitation thing;

[0029] (4) adding concentration to the precipitate in the previous step is 3% sodium acetate to dissociate, then add 95% ethanol 5 times the volume of the dissociated solution, so that the final concentration of etha...

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PUM

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Abstract

The invention discloses a making method of chondroitin sulfate, which comprises the following steps: grinding chondroitin sulfate; enzymolyzing through 2709 alkaline proteinase; filtering; decoloring; sedimenting liquid through cetylpiridinium chloride; dissociating the sediment; sedimenting through alcohol; drying; obtaining the product.

Description

technical field [0001] The invention relates to a method for preparing chondroitin sulfate, in particular to a method for directly preparing chondroitin sulfate from animal cartilage. Background technique [0002] Chondroitin sulfate is an acidic mucopolysaccharide prepared from animal cartilage tissue. It is mostly present in animal cartilage, nasal bone, throat bone, cattle, horse septum and trachea, and shark cartilage. It is an acidic mucopolysaccharide. Under the action of 2709 alkaline protease, mucopolysaccharide can be dissociated and released from the protein in cartilage tissue. Chondroitin sulfate can be clinically used to prevent atherosclerosis, improve immunity, treat rheumatism, nephritis and hearing impairment caused by streptomycin. As a food additive, chondroitin sulfate can be used for emulsification, moisturizing and odor removal of food. Applied in cosmetics, it can regulate skin cell metabolism, promote nutrient absorption, maintain skin moisture and ...

Claims

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Application Information

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IPC IPC(8): C08B37/08
Inventor 张万山杨华英张志武屈巍朱月华刘兰花曾英万偲李菊根
Owner NANCHANG WANHUA BIOCHEM PHARMA
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