Enzymolysis and fermentation combined method for improving feeding nutritional value of rapeseed meal

A technology of nutritional value and rapeseed meal, which is applied in the field of feed processing, can solve the problems of loss of intermediate metabolites, consumption of large nutrient production capacity, and no biological fermentation method, etc., so as to increase the amount of addition, reduce the cost of breeding, and alleviate the resource shortage. depletion effect

Inactive Publication Date: 2016-04-20
侯文燕
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above studies have certain effects on the removal of phytic acid, they all use aerobic bacteria for aerobic fermentation. Compared with anaerobic fermentation, aerobic fermentation will consume a lot of nutrient production capacity, and at the same time, intermediate metabolites w

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A new process for improving the nutritional value of rapeseed meal by utilizing enzymolysis method and microbial fermentation method, the specific preparation steps are as follows:

[0033] (1) Crushing: crush the rapeseed meal and pass it through a 40-60 mesh sieve;

[0034] (2) Ingredients: according to rapeseed meal 96%, molasses 2%, NH 4 Cl1.0%, KH 2 PO4.3H 2 O0.5%, MgSO 4 .7H 2 Weigh the material at a ratio of 0.5%, mix it evenly, and adjust the moisture content of the material to be 15-20%;

[0035](3) Enzyme addition: Alkaline protease was added exogenously according to the mass ratio of rapeseed meal to 6%;

[0036] (4) Aspergillus niger activation: Activation of Aspergillus niger in Chapman’s medium at a ratio of 1:30;

[0037] (5) Inoculation: Inoculate Aspergillus niger into the fermentation medium according to the inoculation ratio of 5%;

[0038] (6) Mixing: Mix the fermentation medium after inserting Aspergillus niger and protease evenly;

[0039] ...

Embodiment 2

[0047] A new process for improving the nutritional value of rapeseed meal by utilizing enzymolysis method and microbial fermentation method, the specific preparation steps are as follows:

[0048] (1) Crushing: crush the rapeseed meal and pass it through a 40-60 mesh sieve;

[0049] (2) Ingredients: according to rapeseed meal 95%, molasses 3%, NH 4 Cl1.0%, KH 2 PO4.3H 2 O0.5%, MgSO 4 .7H 2 Weigh the material at a ratio of 0.5%, mix it evenly, and adjust the moisture content of the material to be 15-20%;

[0050] (3) Enzyme addition: Alkaline protease was added exogenously according to the mass ratio of rapeseed meal to 7%;

[0051] (4) Activation of Aspergillus niger: activate Aspergillus niger in Chapkin's medium at a ratio of 1:40;

[0052] (5) Inoculation: Inoculate Aspergillus niger into the fermentation medium according to the inoculation ratio of 6%;

[0053] (6) Mixing: Mix the fermentation medium after inserting Aspergillus niger and protease evenly;

[0054] (...

Embodiment 3

[0062] A new process for improving the nutritional value of rapeseed meal by utilizing enzymolysis method and microbial fermentation method, the specific preparation steps are as follows:

[0063] (1) Crushing: crush the rapeseed meal and pass it through a 40-60 mesh sieve;

[0064] (2) Ingredients: according to rapeseed meal 94%, molasses 4%, NH 4 Cl1.0%, KH 2 PO4.3H 2 O0.5%, MgSO 4 .7H 2 Weigh the material at a ratio of 0.5%, mix it evenly, and adjust the moisture content of the material;

[0065] (3) Enzyme addition: Alkaline protease was added exogenously according to the mass ratio of rapeseed meal to 8%;

[0066] (4) Activation of Aspergillus niger: Activation of Aspergillus niger in Chapkin's medium at a ratio of 1:50;

[0067] (5) Inoculation: Inoculate Aspergillus niger into the fermentation medium according to the inoculation ratio of 8%;

[0068] (6) Mixing: Mix the fermentation medium after inserting Aspergillus niger and protease evenly;

[0069] (7) Solid...

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PUM

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Abstract

The invention discloses a new process utilizing enzymolysis and microbiological fermentation to improve the feeding nutritional value of rapeseed meal. The method is characterized in that: the process combining the enzymolysis of exogenously added alkaline protease and microbial two-step anaerobic fermentation is employed to prepare a high value-added fermented rapeseed meal product that has high protein content, is rich in polypeptide, small peptide, amino acid, organic acid, probiotics and other nutrients, and has low content of phytic acid, tannin and other antinutritional factors. The fermented rapeseed meal product has a crude protein content of more than or equal to 50%, an acid soluble protein content of greater than or equal to 10%, a degree of hydrolysis of more than or equal to 35%, an organic acid content of more than or equal to 5%, a phytic acid content of less than or equal to 0.1%, and a volatile basic nitrogen content of less than or equal to 100mg/100g, the feed utilization rate is improved, and the culture cost is reduced. The fermented rapeseed meal can be applied to aquatic feed, enhances the adding amount of fermented rapeseed meal in aquatic feed, and can partially replace the application of fish meal, soybean meal and the like. In addition, the method provided by the invention adopts solid static fermentation, also the fermentation process has no need for sterilization, the production process is simple, the production cost is low, and the industrial production prospect is broad.

Description

technical field [0001] The invention relates to a new process for improving the feeding nutritional value of rapeseed meal by using an enzymatic hydrolysis method and a microbial fermentation method, and belongs to the field of feed processing. Background technique [0002] Protein raw materials are the foundation of the development of the feed industry. The shortage and high price of protein raw materials in my country have become one of the bottlenecks in the development of animal husbandry and aquaculture. At present, with the improvement of people's living standards and the emphasis on the quality of life, the demand for meat, eggs, milk, and aquatic products is increasing day by day. The production of grain and feed is facing severe challenges, and feed protein resources will become increasingly scarce. It is imminent to expand feed resources and develop unconventional feed. The important strategy to alleviate the contradiction is to improve the utilization rate of the...

Claims

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Application Information

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IPC IPC(8): A23K1/14A23K1/02A23K1/165A23K1/175
Inventor 候文燕
Owner 侯文燕
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