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A process for improving the nutritional value of cottonseed meal by using microbial fermentation

A microbial fermentation method and nutritional value technology, applied in the field of bioengineering, can solve the problems of high production cost, long fermentation cycle, and weak industrial transformation ability

Active Publication Date: 2021-05-14
桂林精成生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A comprehensive analysis of the existing research results shows that although a series of research results have been obtained in the preparation of cottonseed protein feed by microbial fermentation, there are still problems such as complex fermentation process, long fermentation cycle, weak industrial transformation ability, high production cost, and high production cost. Technical drawbacks such as nutritional indicators can be further improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A process for improving the nutritional value of cottonseed meal by using microbial fermentation, the specific process steps include:

[0033] (1) Pretreatment: select cotton meal without mildew, crush it through a 40-mesh sieve, and adjust the moisture content of cotton meal to 40-60%;

[0034] (2) Seed liquid preparation:

[0035] Under the technical scheme, the saccharomycete is a mixed bacterium of Candida utilis and Kluyveromyces fragilis, and its dosage ratio is 7:3, and the lactic acid bacterium is Lactobacillus acidophilus;

[0036] ① The preparation method of the seed liquid of Aspergillus niger is as follows: take fresh bran, sieve the fine powder with a 60-mesh sieve, add water according to the ratio of bran: water = 1: 1.0-1.3, and mix well until there is no dry powder and no agglomeration. After mixing well, divide into 2000mL conical flasks, each 2000mL conical flask contains about 100g of wet bran, sterilize at 0.1MPa gauge pressure for 40-60min, cool to...

Embodiment 2

[0046] A process for improving the nutritional value of cottonseed meal by using microbial fermentation, the specific process steps include:

[0047] (1) Pretreatment: select cotton meal without mildew, crush it through a 40-mesh sieve, and adjust the moisture content of cotton meal to 40-60%;

[0048] (2) Seed liquid preparation:

[0049] Under the technical scheme, the saccharomyces is beer yeast, and the lactic acid bacteria are Lactobacillus bulgaricus;

[0050] ① The preparation method of the seed liquid of Aspergillus niger is as follows: take fresh bran, sieve the fine powder with a 60-mesh sieve, add water according to the ratio of bran: water = 1: 1.0-1.3, and mix well until there is no dry powder and no agglomeration. After mixing well, divide into 2000mL conical flasks, each 2000mL conical flask contains about 100g of wet bran, sterilize at 0.1MPa gauge pressure for 40-60min, cool to 35°C, put 1-2 Ring the activated spores of Aspergillus oryzae cv-8 and Aspergillu...

Embodiment 3

[0060] A process for improving the nutritional value of cottonseed meal by using microbial fermentation, the specific process steps include:

[0061] (1) Pretreatment: select cotton meal without mildew, crush it through a 40-mesh sieve, and adjust the moisture content of cotton meal to 40-60%;

[0062] (2) Seed liquid preparation:

[0063] Under the technical scheme, the yeast is Saccharomyces cerevisiae, and the lactic acid bacteria are Lactobacillus plantarum;

[0064] ① The preparation method of the seed liquid of Aspergillus niger is as follows: take fresh bran, sieve the fine powder with a 60-mesh sieve, add water according to the ratio of bran: water = 1: 1.0-1.3, and mix well until there is no dry powder and no agglomeration. After mixing well, divide into 2000mL conical flasks, each 2000mL conical flask contains about 100g of wet bran, sterilize at 0.1MPa gauge pressure for 40-60min, cool to 35°C, put 1-2 Ring the activated spores of Aspergillus oryzae cv-8 and Asper...

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Abstract

The invention discloses a process for improving the nutritional value of cottonseed meal by using microbial fermentation, integrating modern mixed-bacteria microbial fermentation technology, enzyme technology, etc., and developing a high protein, high amino acid content, low free gossypol content, high feed Cotton meal protein feed with a high rate of return, the specific process is a two-step process of mixed-bacteria aerobic fermentation and mixed-bacteria anaerobic fermentation, in which the aerobic fermentation bacteria group includes Aspergillus niger, yeast, Bacillus subtilis, and Bacillus pyrolyticus; Aerobic fermentative flora include Clostridium butyricum and lactic acid bacteria. The process adopts multi-bacteria mixed fermentation. Through the combination of different metabolic abilities, complex metabolic functions that are difficult for a single strain can be completed. The collaborative fermentation between microorganisms plays an important role in the effective transformation of various raw materials and the improvement of the quality of protein feed. The invention has the advantages of good product efficacy, simple and efficient process, short fermentation cycle, low production cost, etc., and is easy to produce on an industrial scale.

Description

technical field [0001] The invention relates to a process for improving the nutritional value of cotton meal by microbial fermentation, in particular to a production process for producing fermented cotton meal with high protein content and high feed return rate by using a variety of probiotics for collaborative fermentation, belonging to the field of bioengineering . Background technique [0002] The shortage of feed resources, especially protein feed, is one of the bottlenecks restricting the development of animal husbandry in my country. How to effectively alleviate the shortage of protein feed resources in my country is a major problem to be solved. Protein raw materials are the foundation of the development of feed industry. The shortage and high price of protein raw materials in my country have become the bottleneck restricting the development of animal husbandry. At present, with the improvement of people's living standards and the emphasis on the quality of life, the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K10/30A23K10/37A23K10/33A23K10/12A23K20/105A23K20/26A23K20/189
CPCA23K10/12A23K10/30A23K10/33A23K10/37A23K20/105A23K20/189A23K20/26Y02P60/87
Inventor 董书阁张顺涛陈希凯张志军
Owner 桂林精成生物科技有限公司
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