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Yak bone protein peptide with DPP-IV inhibitory activity and preparation method thereof

A technology for DPP-IV and inhibitory activity, which is applied in the field of yak bone protein peptide and its preparation, can solve the problems of no relevant research on the peptide, and achieve the effect of simple preparation method, excellent DPP-IV inhibition and anti-oxidation function

Active Publication Date: 2017-09-08
人民国肽集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, there is no relevant research on the preparation of peptides with DPP-IV inhibitory activity from yak bone

Method used

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  • Yak bone protein peptide with DPP-IV inhibitory activity and preparation method thereof
  • Yak bone protein peptide with DPP-IV inhibitory activity and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Example 1: Preparation of yak bone protein peptide with DPP-IV inhibitory and antioxidant functions

[0067] (1) Choose 100 grams of frozen fresh yak bones, wash them with clean water that meets the drinking water hygiene standards, and use a ox bone crusher to beat the yak bones into bone particles after cleaning the yak bones, add water twice the total amount of yak bone particles in the Treat at 121°C for 5 hours, obtain yak bone cooking liquid with bone residue removed by centrifugation, cool to 20°C, and remove fat through a liquid separation tank to obtain yak bone protein liquid.

[0068] (2) Adjust the temperature of the yak bone protein solution obtained in (1) to 65° C., and use an ultrasonic generator to process ultrasonic waves (frequency 80 kH) for 15 minutes.

[0069] (3) regulating the protein content in the yak bone protein liquid is 8%, according to the percentage by weight of protein weight 0.8% in the yak bone protein liquid, add composite protease an...

Embodiment 2

[0073] Example 2: Preparation of yak bone protein peptide with DPP-IV inhibitory and antioxidant functions

[0074] (1) Choose 500 grams of frozen fresh yak bones, wash them with clean water that meets the drinking water hygiene standards, and use a bone crusher to beat the yak bones into bone particles after cleaning the yak bones, add 1.5 times the total amount of yak bone particles in water Treat at 130°C for 3 hours, centrifuge and filter to obtain yak bone cooking liquid without bone slag, cool to 30°C, and remove fat through a liquid separation tank to obtain yak bone protein liquid.

[0075] (2) Adjust the temperature of the yak bone protein solution obtained in (1) to 70° C., and use an ultrasonic generator to process ultrasonic waves (frequency 70 kH) for 20 minutes.

[0076] (3) regulating the protein content in the yak bone protein liquid is 9%, adding compound protease according to the percentage by weight of protein weight 1.0% in the yak bone protein liquid and c...

Embodiment 3

[0080]Example 3: Preparation of yak bone protein peptide with DPP-IV inhibitory and antioxidative functions

[0081] (1) Choose 1000 grams of frozen fresh yak bones, wash them with clean water that meets the drinking water hygiene standards, and use a ox bone grinder to beat the yak bones into bone particles after cleaning the yak bones, add 3.0 times the total amount of yak bone particles in water Treat at 125°C for 3 hours, then centrifuge to obtain yak bone high-temperature cooking liquid without bone slag, cool to 25°C, and remove fat through a liquid separation tank to obtain yak bone protein liquid.

[0082] (2) Adjust the temperature of the yak bone protein solution obtained in (1) to 75° C., and use an ultrasonic generator to process ultrasonic waves (frequency 60 kH) for 25 minutes.

[0083] (3) regulating the protein content in the yak bone protein liquid is 9%, adding the compound protease according to the weight percentage of 0.6% of the protein weight in the yak b...

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Abstract

The invention provides yak bone protein peptide with DPP-IV inhibitory activity and a preparation method thereof. The yak bone protein peptide provided by the invention is optimally prepared from the enzymolysis products by conducting multiple-step enzymolysis by multilevel compound protease on the yak bone protein prepared from yak bone, and the compound protease contains alkali protease, neutral proteinase, trypsin and flavourzyme. The yak bone protein peptide provided by the invention has excellent DPP-IV inhibition and antioxidation functions, and can be used as a DPP-IV inhibitor and antioxidation raw material to be applied to special foods and nutritional foods.

Description

technical field [0001] The invention relates to the field of animal food processing, more specifically, to yak bone protein peptide with DPP-IV inhibition and anti-oxidation functions and a preparation method. Background technique [0002] Hypoglycemic functional factors refer to biologically active components that can reduce blood sugar concentration and improve symptoms of diabetic patients. At present, there are many natural product-type hypoglycemic factors, mineral-type hypoglycemic factors, and vitamin-type hypoglycemic factors with different mechanisms of action. Natural product hypoglycemic factors can be divided into flavonoids, [0003] Active polysaccharides, alkaloids, saponins, terpenes, polypeptides, etc. Since the artificial synthesis of insulin, various oral or injectable drugs such as sulfonylureas, biguanides, insulin sensitizers, and sugar inhibitors have come out one after another. However, chemical drugs often produce certain toxic and side effects, an...

Claims

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Application Information

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IPC IPC(8): C07K7/06C07K14/47C12P21/06A61K38/08A61P39/06A23L33/18A23C9/152
CPCA23C9/1526A23V2002/00A61K38/00A23L33/18C07K7/06C07K14/47C12P21/06A23V2200/326A23V2200/328A23V2200/322A23V2200/30A23V2250/55
Inventor 张恒罗永康
Owner 人民国肽集团有限公司
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