The invention belongs to the field of marine organism small-molecular active peptides, and particularly relates to an anti-oxidation and DPP-IV inhibition active peptide derived from apostichopus japonicus by enzymolysis. The amino acid sequence of the anti-oxidation and DPP-IV inhibition active peptide is Ser-Arg-Pro-Gln-Tyr-Pro-Gln-Tyr-Pro-Ser. The anti-oxidation and DPP-IV inhibition active peptide is prepared by the following steps: adding water into fresh apostichopus japonicus for homogenizing, and placing the homogenate in an enzymolysis tank; performing enzymolysis with compound protease to obtain enzymatic hydrolysate; treating the enzymatic hydrolysate with a membrane separation technology to obtain a small-molecular active peptide crude product; separating the crude product through Sephadex LH-20 to obtain a small-molecular active peptide. The purity of the small-molecular active peptide as measured by adopting RP-HPLC (Reversed-Phase High Performance Liquid Chromatography)is greater than 95 percent, and the amino acid composition of the active peptide is measured by high performance liquid chromatography/mass spectrometry, so that the amino acid sequence of the small-molecular peptide is determined finally. The active small-molecular peptide derived from the apostichopus japonicus has the advantages of small molecular weight, simple separating-purifying steps, easiness in preparation, high purity and higher oxidization resistance and DPP-IV inhibition activity, has the characteristics of naturalness, safety and high efficiency, can be applied to the preventionand treatment of relevant diseases such as diabetes mellitus as an antioxidant as well as a DPP-IV inhibitor, and has a wide application prospect in the fields of foods, health care products and medicines.