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Method for preparing protein hydrolyzate from abalone viscera through complex enzyme hydrolysis

A protein hydrolyzate and compound enzymatic hydrolysis technology are applied in the field of compound enzymatic hydrolysis of abalone viscera to prepare protein hydrolyzate, which can solve problems such as low protein hydrolysis yield, and achieve the effects of high hydrolysis rate, complete hydrolysis, and easy control of process conditions.

Inactive Publication Date: 2010-12-15
福州日兴水产食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using a single proteolytic enzyme, the yield of protein hydrolysis is low

Method used

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  • Method for preparing protein hydrolyzate from abalone viscera through complex enzyme hydrolysis

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] 1. After the abalone viscera is taken out and cleaned, it is roughly crushed with a beater, and then put into a colloid mill for micronization treatment for 10 minutes. The speed of the colloid mill is 3000 rpm to obtain abalone viscera slurry;

[0016] 2. Take a certain amount of abalone viscera slurry, add water and stir well, and adjust the solid-liquid ratio to 1:1;

[0017] 3. Add sodium hydroxide solution with a mass concentration of 1mol / L to adjust the pH of the mixture to 8;

[0018] 4. Add the compound enzyme uniformly mixed with alkaline protease and papain, heat for enzymatic hydrolysis treatment, stir once every 10-15 minutes to obtain enzymatic hydrolyzate, wherein the weight ratio of alkaline protease and papain is 2 : 1, the addition amount of compound enzyme is 0.5%, the enzymatic hydrolysis treatment temperature is 45 ℃, and the time is 5h;

[0019] 5. Put the hydrolyzate in a jacketed pot, heat to 85°C, inactivate the enzyme for 10 minutes, inactivat...

Embodiment 2

[0023] 1. After the abalone viscera is taken out and cleaned, it is roughly crushed with a beater, and then put into a colloid mill for micronization treatment for 10 minutes. The speed of the colloid mill is 3000 rpm to obtain abalone viscera slurry;

[0024] 2. Take a certain amount of abalone viscera slurry, add water and stir well, and adjust the solid-liquid ratio to 3:2;

[0025] 3. Add sodium hydroxide solution with a mass concentration of 1mol / L to adjust the pH of the mixture to 7.5;

[0026] 4. Add the compound enzyme uniformly mixed with alkaline protease and papain, heat for enzymatic hydrolysis treatment, and stir once every 10-15 minutes to obtain an enzyme hydrolyzate, wherein the weight ratio of alkaline protease and papain is 1 : 1, the addition amount of compound enzyme is 0.6%, the enzymatic hydrolysis treatment temperature is 50℃, and the time is 4.5h;

[0027] 5. Put the hydrolyzate in a jacketed pot, heat to 85°C, inactivate the enzyme for 10 minutes, in...

Embodiment 3

[0031] 1. After the abalone viscera is taken out and cleaned, it is roughly crushed with a beater, and then put into a colloid mill for micronization treatment for 10 minutes. The speed of the colloid mill is 3000 rpm to obtain abalone viscera slurry;

[0032] 2. Take a certain amount of abalone viscera slurry, add water and stir well, and adjust the solid-liquid ratio to 3:1;

[0033] 3. Add sodium hydroxide solution with a mass concentration of 1mol / L to adjust the pH of the mixture to 7.5;

[0034] 4. Add the compound enzyme uniformly mixed with alkaline protease and papain, heat for enzymatic hydrolysis treatment, stir once every 10-15 minutes to obtain enzymatic hydrolyzate, wherein the weight ratio of alkaline protease and papain is 2 : 1, the addition amount of compound enzyme is 0.7%, the enzymatic hydrolysis treatment temperature is 50 ℃, and the time is 5h;

[0035] 5. Put the hydrolyzate in a jacketed pot, heat to 85°C, inactivate the enzyme for 10 minutes, inactiv...

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PUM

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Abstract

The invention discloses a method for preparing protein hydrolyzate from abalone viscera through complex enzyme hydrolysis, bitterness shielding and other technologies. Complex enzymes comprise alkali protease and papain. The protein hydrolyzate is prepared from abalone viscera by cleaning, pulping, hydrolyzing with the enzymes, inactivating the enzymes and shielding bitterness by using ethyl maltol in sequence. The method effectively utilizes a side product, namely viscera, generated during abalone processing, and the bitterness of the protein hydrolyzate is avoided; the prepared protein hydrolyzate has high content of free amino acid, high absorptivity of human body, rich nutrition and delicious flavor, and can be used as an additive to be widely applied to products such as cans, sauces,fodders, plant nutrient solutions and the like.

Description

technical field [0001] The invention specifically relates to a method for preparing protein hydrolyzate by compound enzymatic hydrolysis of abalone viscera. Background technique [0002] Abalone is delicious and nutritious. Every 100 grams of fresh abalone contains 12.6 grams of protein, 0.8 grams of fat, and 6.6 grams of carbohydrates. It also contains calcium, phosphorus, iron, zinc and other minerals and vitamins needed by the human body. "Diet Therapeutic Materia Medica" records that abalone enters the liver to clear blood stasis, enters the intestines to cleanse dirt, does not hurt vitality, strengthens yang, and generates a hundred pulses. Abalone has high nutritional value and medicinal value, and is one of the organisms with the most comprehensive amino acid content and the lowest fat and cholesterol content in the ocean. [0003] The viscera of abalone are often discarded as leftovers, and the leftovers discarded during the processing of canned products account for...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/04
Inventor 江铭福
Owner 福州日兴水产食品有限公司
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