The invention discloses a preparation method of Chinese wolfberry powder, comprising the steps: using fresh or dry wolfberries as main raw materials; obtaining a wolfberry powder product by processing the fresh or dry wolfberries through the procedures of picking, cleaning or soaking, protecting color, pulping, grinding rubber, secondary processing, blending, homogenizing, sterilizing, concentrating, dry spraying, drying fog, collecting powder, sieving, packing and storing products, wherein the secondary processing procedure comprises the steps: reprocessing filtered coarse residues; decomposing the filtered coarse residues by adding pectinase; reprocessing the filtered coarse residues after being returned into a colloid mill; processing non-enzymatic coarse residues by adopting micronization to obtain fine powder; recovering the fine powder into a powder collecting bin; and then, obtaining a wolfberry powder product by processing the fine powder through the procedures of sieving, packing and storing products. The Chinese wolfberry powder has good color, high solubility, good mouth feel, no side effect, short production period and high profit. The preparation method of Chinese wolfberry powder provides high-quality powdered food for increasing the physical quality of humans and can be widely applied to the industries of food, health products, and the like.