Preparation method of compound microbial seaweed fertilizer
A technology of compound microorganisms and seaweed fertilizer, which is applied in the field of fertilizers, can solve problems such as difficulties, damage to seaweed nutrients, and environmental pollution, and achieve the effects of promoting germination and growth, reducing the use of pesticides, and improving aggregate structure
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Embodiment 1
[0036] 1. A strain of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) WB1, the strain preservation number is: CGMCC No.8873, Gram stain positive, rod-shaped, the surface of the colony is rough, with bulges, irregular edges, and can degrade seaweed polysaccharides. Secretes a variety of glycosidases and proteases, and has a wide range of antibacterial activities.
[0037] 2. A strain of Bacillus subtilis (Bacillus subtilis) ABTNL-2, strain preservation number: CGMCC NO.10038, Gram stain positive, rod-shaped, relatively large colony, rough surface, irregular edges, and bacterial growth Active substances such as subtilisin, polymyxin, nystatin, and gramicidin produced in the process have obvious inhibitory effects on various pathogenic bacteria.
[0038] 3. After activating the two bacillus strains in step 1 and step 2, inoculate them into the seaweed fermentation medium with an equal inoculation amount of 5% (V / V) respectively, and semi-solid fermentation culture to obta...
Embodiment 2
[0059] 1. A strain of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) WB1, the strain preservation number is: CGMCC No.8873, Gram stain positive, rod-shaped, the surface of the colony is rough, with bulges, irregular edges, and can degrade seaweed polysaccharides. Secretes a variety of glycosidases and proteases, and has a wide range of antibacterial activities.
[0060] 2. A strain of Bacillus subtilis (Bacillus subtilis) ABTNL-2, strain preservation number: CGMCC NO.10038, Gram stain positive, rod-shaped, relatively large colony, rough surface, irregular edges, and bacterial growth Active substances such as subtilisin, polymyxin, nystatin, and gramicidin produced in the process have obvious inhibitory effects on various pathogenic bacteria.
[0061] 3. After activating the two strains of bacillus in step 1 and step 2, inoculate them into the seaweed fermentation medium with an equal inoculation amount of 5% (V / V) respectively, and obtain a fermented liquid through se...
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