Preparation method of compound microbial seaweed fertilizer

A technology of compound microorganisms and seaweed fertilizer, which is applied in the field of fertilizers, can solve problems such as difficulties, damage to seaweed nutrients, and environmental pollution, and achieve the effects of promoting germination and growth, reducing the use of pesticides, and improving aggregate structure

Active Publication Date: 2015-07-15
DALIAN UNIV OF TECH
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  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

However, it is still difficult to use microorganisms to fully ferment seaweed to effectively release its nutrients, because seaweed contains a large amount of polysaccharides that are difficult to decompose and high concentrations of salt
At this stage, there are still many defects in the preparation of seaweed fertilizer by fermentation. First, there is a lack of ef

Method used

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  • Preparation method of compound microbial seaweed fertilizer
  • Preparation method of compound microbial seaweed fertilizer

Examples

Experimental program
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Effect test

Embodiment 1

[0036] 1. A strain of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) WB1, the strain preservation number is: CGMCC No.8873, Gram stain positive, rod-shaped, the surface of the colony is rough, with bulges, irregular edges, and can degrade seaweed polysaccharides. Secretes a variety of glycosidases and proteases, and has a wide range of antibacterial activities.

[0037] 2. A strain of Bacillus subtilis (Bacillus subtilis) ABTNL-2, strain preservation number: CGMCC NO.10038, Gram stain positive, rod-shaped, relatively large colony, rough surface, irregular edges, and bacterial growth Active substances such as subtilisin, polymyxin, nystatin, and gramicidin produced in the process have obvious inhibitory effects on various pathogenic bacteria.

[0038] 3. After activating the two bacillus strains in step 1 and step 2, inoculate them into the seaweed fermentation medium with an equal inoculation amount of 5% (V / V) respectively, and semi-solid fermentation culture to obta...

Embodiment 2

[0059] 1. A strain of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) WB1, the strain preservation number is: CGMCC No.8873, Gram stain positive, rod-shaped, the surface of the colony is rough, with bulges, irregular edges, and can degrade seaweed polysaccharides. Secretes a variety of glycosidases and proteases, and has a wide range of antibacterial activities.

[0060] 2. A strain of Bacillus subtilis (Bacillus subtilis) ABTNL-2, strain preservation number: CGMCC NO.10038, Gram stain positive, rod-shaped, relatively large colony, rough surface, irregular edges, and bacterial growth Active substances such as subtilisin, polymyxin, nystatin, and gramicidin produced in the process have obvious inhibitory effects on various pathogenic bacteria.

[0061] 3. After activating the two strains of bacillus in step 1 and step 2, inoculate them into the seaweed fermentation medium with an equal inoculation amount of 5% (V / V) respectively, and obtain a fermented liquid through se...

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Abstract

The invention discloses a preparation method of a compound microbial seaweed fertilizer. Screened bacillus amylolique faciens WB1 with high alginate lyase activity and bacillus subtilis ABTNL-2 capable of producing various of ablastins are used for fermenting seaweed subjected to enzyme-aid micronization treatment, so as to generate a great deal of micromolecular special-effect metabolites capable of promoting growth of crops, such as alginooligosaccharide, thereby improving the bioactivity of the seaweed fertilizer. Moreover, the advantage that the bacillus amylolique faciens WB1 and the bacillus subtilis ABTNL-2 are capable of inhibiting various pathogenic bacteria of crops can be put into a full play; the compound microbial seaweed fertilizer has a high nutrition added value and has the pesticide-fertilizer integrated functions of improving soil properties and preventing various diseases.

Description

technical field [0001] The invention relates to the technical field of fertilizers, in particular to a method for preparing compound microbial seaweed fertilizer. Background technique [0002] The long-term and extensive use of chemical fertilizers has gradually caused problems such as soil barrenness, compaction, and nutritional imbalances. The long-term abuse of pesticides has also led to serious problems such as enhanced resistance of pathogenic bacteria and environmental pollution. In order to ensure the high-speed, sustainable and green development of agriculture, the Ministry of Agriculture of my country has paid more and more attention to the use of organic fertilizers, and proposed to gradually reduce the use of chemical fertilizers and pesticides in agriculture. Seaweed is rich in a variety of nutrients and is an excellent raw material for the manufacture of natural organic fertilizers. Its fermented and degraded small molecule oligosaccharides contain natural plant...

Claims

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Application Information

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IPC IPC(8): C05G3/00C05G3/04C05F17/00C05G3/80
CPCY02W30/40
Inventor 王熙涛徐永平续彦龙龚改林王丽丽李晓宇车鉴
Owner DALIAN UNIV OF TECH
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