Beche-de-mer ovum nurture and preparation method thereof

A technology of sea cucumber eggs and nutritional products, which is applied in the fields of extracting nutritious food from marine animals, enzymatic hydrolysis, freeze-drying, and chemical ion exchange. It can solve the problems of reporting, waste of rare resources, and underutilization of nutrients and functional factors. Achieve the effect of improving safety and quality and reducing pollution

Inactive Publication Date: 2009-02-11
DALIAN POLYTECHNIC UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In aquatic product processing enterprises, sea cucumber flowers have been treated as waste during sea cucumber processing so far, and a large number of nutrients and functional factors in sea cucumber eggs have

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Separate 1 kg of sea cucumber eggs and intestines from the body wall, cut them into pieces, and wash them. Stir and mix the shredded sea cucumber eggs and intestines with 50 kg of water, adjust the pH to 5, and then place them in a water bath at 50°C for 6 hours. . Add 1 kg of pepsin to the sea cucumber eggs and intestines treated with endogenous enzymes, and use continuous stirring at 30°C for 2 hours to enzymolyze the above pH value during hydrolysis; then adjust the pH to neutral, inactivate the enzyme at high temperature, and cool to Centrifuge at 3500r / min for 30min at room temperature to obtain a supernatant, repeat the pepsin treatment step with 0.5kg of precipitate and 5kg of water, and combine the centrifuged supernatants for later use; put the 50L centrifuged supernatant obtained above at 35°C Concentrate under reduced pressure to 10 L; the above concentrate is treated with a weakly acidic cation exchange series column with a column volume of 1000 ml and a flo...

Embodiment 2

[0028] Separate 10kg of sea cucumber eggs and intestines from the body wall, cut them into pieces, and wash them. Stir and mix the shredded sea cucumber eggs and intestines with 100kg of water, adjust the pH to 7, and then place them in a water bath at 30°C for 2 hours. Add 2 kg of pepsin to the sea cucumber eggs and intestines treated with endogenous enzymes, and use continuous stirring at 50°C for 5 hours to enzymolyze the above pH value; Centrifuge at 4000r / min for 10min at room temperature to obtain a supernatant, repeat the pepsin treatment step with 8kg of precipitate and 80kg of water, and combine the centrifuged supernatants for later use; Concentrate under reduced pressure to 50L; the above concentrated solution is treated with a weakly acidic cation exchange series column with a column volume of 5000ml and a flow rate of 1000ml / min. Freeze-dry the collected concentrate, adjust the freezing temperature to 30°C, the cold trap temperature to -25°C, and the vacuum degree...

Embodiment 3

[0030] Separate 100g of sea cucumber eggs and intestines from the body wall, cut them into pieces, and wash them. Stir and mix the shredded sea cucumber eggs and intestines with 8000g of water, adjust the pH to 3, and then place them in a water bath at 45°C for 3 hours. Add 120g of pepsin to the sea cucumber eggs and intestines treated with endogenous enzymes, and use continuous stirring at 50°C for 3 hours to enzymolyze the above pH value during hydrolysis; then adjust the pH to neutral, high temperature to inactivate the enzyme, and cool to Centrifuge at room temperature at 5000r / min for 15min to obtain a supernatant, add 70g of the precipitate and add 140g of water to repeat the pepsin treatment step, and combine the centrifuged supernatants for later use; decompress the 7800ml centrifuged supernatant obtained above at 60°C Concentrate to 800ml; the above concentrate is treated with a weakly basic cation exchange series column with a column volume of 8000ml and a flow rate o...

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PUM

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Abstract

The invention discloses a sea cucumber egg nutriment and a preparation method thereof. The sea cucumber egg nutriment is white or light yellow hydroscopic powder free of odor and taste and highly soluble to water and ethanol. The preparation method comprises sea cucumber egg and intestine breaking and enzymolysis, metal ion removal through ion exchange, and freeze drying, wherein the enzymolysis employs conjugated enzymes of exogenous enzymes and endogenous enzymes or single exogenous enzymes; single exogenous enzyme enzymolysis refers to the enzymolysis using single pepsinase or using the mixture of trypsinase, dispase and alkaline proteinase. The invention not only makes wastes in sea cucumber processing with traditional methods into an invigorant rich in nutrition but also reduces pollution of the wastes to the environment. The processing method is simple and reasonable and can be industrialized easily.

Description

technical field [0001] The invention belongs to the field of extracting nutritious food from marine animals, and relates to the technical fields of enzymatic hydrolysis, chemical ion exchange and freeze-drying. Background technique [0002] Sea cucumber eggs, also known as sea cucumber flowers, have extremely high nutritional value. Sea cucumber eggs are generally collected from sea cucumbers in May and June each year. Because of the short collection time and low yield, sea cucumber eggs are very valuable and rare nourishing treasures. In aquatic product processing enterprises, sea cucumber flowers have been treated as waste during sea cucumber processing so far, and a large number of nutrients and functional factors in sea cucumber eggs have not been fully utilized, resulting in a complete waste of this rare resource. [0003] At present, there are no related products of sea cucumber eggs, and no relevant literature and information reports have been seen. Contents of the ...

Claims

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Application Information

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IPC IPC(8): A23L1/333A23L1/015A23L1/025A23B4/03A23B4/037A23P1/06A23L5/20A23L5/30A23L17/50
Inventor 朱蓓薇姜春家董秀萍于建伟周大勇李冬梅陈雪娇赵鸿霞
Owner DALIAN POLYTECHNIC UNIVERSITY
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