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Lactobacillus rhamnosus E2 for producing hyaluronic acid and application of lactobacillus rhamnosus E2

A technology of Lactobacillus rhamnosus and hyaluronic acid, applied in the field of microorganisms, can solve the problems of lack of scientific research evidence for the function of probiotics, affect the promotion of probiotics and their products, and rely on imports of probiotic strains, so as to promote the development of zebrafish The effects of repairing caudal fin damage, alleviating skin aging, and reducing ROS levels

Active Publication Date: 2022-07-26
FOSHAN LANGSEE BIOTECHNOLOGY CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, there are few studies and applications of probiotics in anti-aging and promoting tissue repair.
The probiotic strains used by domestic production enterprises have long relied on imports, and foreign strains may not be suitable for the physiological conditions of the gastrointestinal tract of Chinese residents
In addition, the function of probiotics lacks strong scientific research evidence, which seriously affects the promotion of probiotics and their products

Method used

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  • Lactobacillus rhamnosus E2 for producing hyaluronic acid and application of lactobacillus rhamnosus E2
  • Lactobacillus rhamnosus E2 for producing hyaluronic acid and application of lactobacillus rhamnosus E2
  • Lactobacillus rhamnosus E2 for producing hyaluronic acid and application of lactobacillus rhamnosus E2

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Isolation, identification and preservation of Lactobacillus rhamnosus E2

[0028] (1) Separation: After gradient dilution, the feces of the long-lived elderly were inoculated into anaerobic blood agar medium and MRS solid medium respectively, and anaerobic culture at 37°C for 48 hours, and single colonies on the plate were picked and streaked to obtain pure colonies. . The pure colonies on the plate were inoculated into MRS liquid medium, cultured anaerobically at 37°C for 12-16h, added 20% glycerol, and stored in a -80°C refrigerator.

[0029] (2) Morphological identification of strains: The screened strains were Gram-stained and observed under a microscope. Gram-positive bacteria were purple and Gram-negative bacteria were red.

[0030] (3) Molecular biological identification of strains: Genomic DNA was extracted from the obtained strains, and the full-length fragments of 16S rDNA were amplified by PCR technology using 16S rDNA universal primers 27F and 149...

Embodiment 2

[0037] Example 2 Determination of the ability of Lactobacillus rhamnosus E2 to produce hyaluronic acid

[0038] Lactobacillus rhamnosus E2 was inoculated into MRS liquid medium, placed in an anaerobic workstation at 37 °C for 14 h, and the concentration of the strain was adjusted to 1 × 10 6 CFU / mL, inoculated in fresh MRS liquid medium by volume fraction 5%, placed in a full-temperature shaker at 37°C, 150r / min for 0, 6, 12, 24, 48, 72 hours, and then used ELISA kit. (Nanjing Herbal Source Biotechnology Co., Ltd.) to detect the hyaluronic acid concentration in the fermentation supernatant of the strain. Each time point was tested 3 times. The experimental data are represented by x±SEM data,

[0039] see the results image 3 . Depend on image 3 It can be seen that Lactobacillus rhamnosus E2 can be fermented to produce hyaluronic acid after 6 hours of inoculation in the medium, and almost reaches the saturation concentration after 72 hours, and the final hyaluronic acid p...

Embodiment 3

[0040] Example 3 Preparation of Lactobacillus rhamnosus E2 fermentation supernatant (extracellular secretion) and bacterial suspension (cell)

[0041] The Lactobacillus rhamnosus E2 was activated and cultured and then inoculated into MRS liquid medium. After culturing at 37 °C for 15 h, the concentration of fermented bacteria was adjusted to 1 × 10 7 CFU / mL, 4°C, 6000r / min centrifugation for 10min to obtain the culture supernatant and bacterial cell precipitate, the supernatant liquid was filtered through a 0.22μm filter to obtain the fermentation supernatant (extracellular secretion); After washing, the cells were resuspended in PBS, and the cell concentration was adjusted to 1 × 10 7 CFU / mL to obtain bacterial suspension (cell).

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PUM

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Abstract

The invention discloses lactobacillus rhamnosus E2 for producing hyaluronic acid and application of the lactobacillus rhamnosus E2, and belongs to the technical field of microorganisms. The preservation number of the lactobacillus rhamnosus E2 for producing hyaluronic acid disclosed by the invention is CGMCC (China General Microbiological Culture Collection Center) No.21770, and the lactobacillus rhamnosus E2 can produce hyaluronic acid under a fermentation condition. The fermentation supernatant and bacterial suspension of the lactobacillus rhamnosus E2 disclosed by the invention can significantly reduce the level of reactive oxygen in the zebra fish body and significantly improve the activity of superoxide dismutase in the zebra fish body in an in-vivo oxidative stress model, and have the potential of being applied to in-vivo oxidation resistance and senescence delaying; in addition, in a zebra fish injury model, zebra fish tail fin injury repair can be remarkably promoted, and the good potential for promoting tissue injury repair is shown. The lactobacillus rhamnosus E2 disclosed by the invention has a huge potential application prospect in the aspects of resisting oxidation, delaying senescence and promoting tissue damage repair.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a hyaluronic acid-producing Lactobacillus rhamnosus E2 and its application. Background technique [0002] Hyaluronic acid (HA), also known as hyaluronic acid, is a linear acidic mucopolysaccharide composed of acetylglucosamine and glucuronic acid disaccharide units alternately connected. Hyaluronic acid, as the body's own substance, widely exists in the joint cavity, skin, vitreous body, cartilage, umbilical cord and other tissues of the human body. With age, the content of hyaluronic acid in the human body will gradually decrease. This is one of the main causes of stiff joints, aging skin, increased wrinkles, vertigo, and many more. A number of studies have proved that oral hyaluronic acid can promote the body's own synthesis of new hyaluronic acid, increase the content of hyaluronic acid in the body, and then improve the moisture of human skin, with anti-oxidation in v...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/26A61K35/747A61P39/06A61K8/99A61Q19/08A61Q19/00A23L29/00A23C9/123C12R1/225
CPCC12P19/26A61K35/747A61P39/06A61K8/99A61Q19/08A61Q19/00A23L29/065A23C9/1234A23V2400/175
Inventor 郑康帝马燕刘彦陈涛张召
Owner FOSHAN LANGSEE BIOTECHNOLOGY CO LTD
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