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Application of Prussian blue nanoparticles in the preparation of drugs for preventing, delaying or treating neurodegenerative diseases

A Prussian blue and nanoparticle technology, applied in the field of biomedicine, can solve the problems of no relevant reports on the research of neurodegenerative diseases, and achieve the effects of improving learning and memory ability, easy surface modification, and easy mass production

Active Publication Date: 2021-07-13
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, there are no relevant reports on the application of Prussian blue nanoparticles in the prevention, delay or treatment of neurodegenerative diseases

Method used

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  • Application of Prussian blue nanoparticles in the preparation of drugs for preventing, delaying or treating neurodegenerative diseases
  • Application of Prussian blue nanoparticles in the preparation of drugs for preventing, delaying or treating neurodegenerative diseases
  • Application of Prussian blue nanoparticles in the preparation of drugs for preventing, delaying or treating neurodegenerative diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] In this example, a non-functional modified Prussian blue nanoparticle was prepared. Its preparation method comprises the following steps:

[0049] (1) Dissolve 0.01mM potassium ferrocyanide and 0.001mM mPEG-COOH (MW5000) in 5mL deionized water respectively, and mix well to obtain a clear solution A; dissolve 0.01mM ferric chloride in 5mL deionized water, Fully dissolve to obtain a clear solution B; add solution B dropwise to solution A, and react at 60°C for 1 hour;

[0050] (2) After the reaction system was lowered to 25° C., reacted for 1 hour, and centrifuged and washed to obtain Prussian blue nanoparticles without functional modification.

[0051] The particle size and potential of the prepared Prussian blue nanoparticles were characterized, and the results showed that the dynamic light scattering particle size was 80nm, and the surface potential was -32mV.

Embodiment 2

[0053] In this example, a single-target modified Prussian blue nanoparticle was prepared. Its preparation method comprises the following steps:

[0054] (1) Dissolve 0.01mM potassium ferrocyanide and 0.001mM mPEG-COOH (MW5000) in 5mL deionized water respectively, and mix well to obtain a clear solution A; dissolve 0.01mM ferric chloride in 5mL deionized water, Fully dissolve to obtain a clear solution B; add solution B dropwise to solution A, and react at 60°C for 1 hour;

[0055] (2) After the reaction system is lowered to 25°C, react for 1 hour, and centrifuge and wash to obtain Prussian blue nanoparticles without functional modification;

[0056] (3) Dissolve the obtained Prussian blue nanoparticles and 0.01mM 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride in 10mL deionized water, and react at 25°C for 15min to obtain a solution D;

[0057] (4) Add 0.01mM N-hydroxysuccinimide and 0.4μM Congo red to solution D, react at 25°C for 24h, centrifuge and wash, and ...

Embodiment 3

[0060] In this example, a double-targeted modified Prussian blue nanoparticle was prepared. Its preparation method comprises the following steps:

[0061] (1) Dissolve 0.01mM potassium ferrocyanide and 0.001mM mPEG-COOH (MW5000) in 5mL deionized water respectively, and mix well to obtain a clear solution A; dissolve 0.01mM ferric chloride in 5mL deionized water, Fully dissolve to obtain a clear solution B; add solution B dropwise to solution A, and react at 60°C for 1 hour;

[0062] (2) After the reaction system is lowered to 25°C, react for 1 hour, and centrifuge and wash to obtain Prussian blue nanoparticles without functional modification;

[0063] (3) Dissolve the obtained Prussian blue nanoparticles and 0.01mM 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride in 10mL deionized water, and react at 25°C for 10min to obtain a solution D;

[0064] (4) Add 0.01mM N-hydroxysuccinimide, 0.005mg transferrin, and 0.5μM Congo red to solution D, react at 25°C for 24 hou...

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Abstract

The invention relates to the application of Prussian blue nanoparticles in the preparation of drugs for preventing, delaying or treating degenerative diseases of the nervous system. The research of the present invention finds that the Prussian blue nanoparticles have remarkable efficacy in preventing, delaying or treating degenerative diseases of the nervous system. The results of cell-level experiments show that Prussian blue nanoparticles can reduce the ROS level in nerve cells stimulated by hydrogen peroxide, and increase the proportion of living cells in nerve cells stimulated by hydrogen peroxide; the results of animal-level experiments show that Prussian blue nanoparticles can significantly Reduce the expression level of oxidative stress markers in the hippocampus of neurodegenerative disease model mice, significantly improve the learning and memory abilities of neurodegenerative disease model mice, and improve motor dysfunction. Moreover, the preparation process of the Prussian blue nanoparticles is simple, easy for mass production, mild reaction conditions, and easy for surface modification.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and relates to a new application of Prussian blue nanoparticles, in particular to an application of Prussian blue nanoparticles in the preparation of drugs for preventing, delaying or treating degenerative diseases of the nervous system. Background technique [0002] Nervous system degenerative diseases are a class of irreversible neurological diseases caused by the loss of neuron cells in the brain and spinal cord, mainly including Alzheimer's disease, Parkinson's disease, amyotrophic lateral sclerosis, Huntington's disease, etc. As society ages, the incidence of neurodegenerative diseases continues to rise, resulting in huge social medical consumption and family pressure. Most neurodegenerative diseases still lack effective treatments, so finding ways to effectively prevent, delay and treat such diseases is an urgent problem to be solved. [0003] Oxidative stress plays a key role in the de...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K33/26A61K9/14A61P25/28A61P25/16A61P25/14A61P25/08A61P25/00A61P25/02
CPCA61K33/26A61K9/14A61P25/28A61P25/16A61P25/14A61P25/08A61P25/00A61P25/02A61K47/54A61K9/0019A61K9/0053A61K9/006A61K9/0014A61K9/0043A61K47/6843A61K47/64A61K47/644A61K47/18
Inventor 常津窦妍赵冬菊唐雨晴
Owner TIANJIN UNIV
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