Saccharomyces cerevisiae engineering strain for high yield of protopanaxadiol and construction method and use

A technology of protopanaxadiol and Saccharomyces cerevisiae, which is applied in the field of high-yielding protopanaxadiol Saccharomyces cerevisiae engineering strains and construction, which can solve the problems of cell growth toxicity and efficient synthesis of unfavorable products
CN110616231AInactive Publication Date: 2019-12-27TIANJIN UNIV
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
TIANJIN UNIV
Publication Date
2019-12-27
Estimated Expiration
Not applicable Β· inactive patent

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Abstract

The invention discloses a saccharomyces cerevisiae engineering strain for high yield of protopanaxadiol and a construction method and use. The construction method of the saccharomyces cerevisiae engineering strain for high yield of protopanaxadiol comprises the following steps: constructing a Pgk1p-YBP1-Cyc1t gene expression cassette, and performing introduction into a ura3 site of a saccharomycescerevisiae engineering strain to obtain the saccharomyces cerevisiae engineering strain W3a-Py for high yield of protopanaxadiol. The experimental results show that the saccharomyces cerevisiae engineering strain for high yield of protopanaxadiol can reduce an intracellular ROS level of the strain by 62.3% (84 h of fermentation), and the yield of protopanaxadiol in a shake flask is increased from1.40 g / L to 1.67 g / L.
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Description

technical field

[0001] The invention belongs to the technical field of bioengineering, and in particular relates to a high-yield protopanaxadiol Saccharomyces cerevisiae engineering strain, a construction method and application. Background technique

[0002] Protopanaxadiol is the active ingredient in ginseng, which has strong anti-tumor activity and can effectively kill and kill tumor cells. Moreover, protopanaxadiol basically has no toxic and side effects on the human body, and can also enhance the body's immunity, and is suitable for postoperative treatment after tumor surgery or chemotherapy. In addition, protopanaxadiol has excellent curative effect and effect in the treatment of coronary heart disease and depression, improving the body's immunity and learning ability.

[0003] In the production of protopanaxadiol, the traditional ginseng extraction method is restricted by the source of raw materials, pesticide residues and environmental protection issues, the producti...

Claims

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