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Application of neomangiferin in vascular endothelial cell protection

A new mangiferin, vascular endothelium technology, applied in the field of medicine, to achieve the effect of increasing total SOD activity, inhibiting oxidative damage and reducing ROS level

Inactive Publication Date: 2017-11-28
TONGHUA HUAXIA PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Nowadays, there are many studies on Anemarrhena extracts, but for neomangiferin, further research is needed

Method used

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  • Application of neomangiferin in vascular endothelial cell protection
  • Application of neomangiferin in vascular endothelial cell protection
  • Application of neomangiferin in vascular endothelial cell protection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1. Culture of EA.Hy926 cells

[0031] EA.Hy926 cells were thawed with DMEM medium containing 10% FBS at 37°C, 5% CO 2 cultivated under conditions. When the cells grew to 90%, they were digested with trypsin-EDTA and passaged. Cells in the logarithmic growth phase were used for experiments.

[0032] 2. Effects on the proliferation of EA.Hy926 cells

[0033] Cells in the logarithmic growth phase were seeded in 96-well culture plates at 10,000 cells / well, with a volume of 100 μL per well.

[0034] After 24 hours, replace with the medium containing neomangiferin and continue culturing for 24 hours. Afterwards, the culture medium in the culture plate was aspirated, and 100 μL of MTT solution (final concentration: 0.5 mg / mL) was added to each well, and placed in the incubator for 4 hours. Aspirate the MTT solution in the culture plate, add 100 μL of DMSO solution to each well, and shake at 37°C for 10 minutes. The OD value was measured on a TECAN Infinite F200 fluoresce...

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PUM

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Abstract

The invention discloses application of neomangiferin in vascular endothelial cell protection. The neomangiferin can reduce the ROS level in vascular endothelial cells and enhance the total SOD activity in the vascular endothelial cells; oxidative damage of the cells is inhibited effectively, and then a new acting pathway and the therapeutic mechanism of the neomangiferin on treatment of cardiovascular diseases and diabetic vascular complications are clear; and bases are provided for the clinical medicine of the neomangiferin.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of neomangiferin in protecting vascular endothelial cells. Background technique [0002] Neomangiferin is isolated from the traditional Chinese medicine Zhimu and is the main active ingredient of Zhimu. Neomangiferin (NMF) is a kind of Xanthone glycosides with molecular formula C 25 h 28 o 16 , the relative molecular mass is 584, and the chemical structural formula is shown in formula (1): [0003] [0004] At present, there are many researches on the water extract of Anemarrhena, such as: Ichiki and Miura et al. (Ichiki, H.; Miura, T.; Kubo, M.; Ishihara, E.; Komatsu, Y.; Tanigawa, K.; Okada, M., New antidiabetic compounds, mangiferin and its glucoside. Biological & Pharmaceutical Bulletin 1998, 21, 1389-1390; Miura, T.; Ichiki, H.; Hashimoto, I.; Kubo, M.; Ishihara, E.; Komatsu, Y.; Okada, M.; Ishida, T., Antidiabetic activity of a xanthone compound, ma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61P9/00A61P3/10A61P27/02A61P13/12
CPCA61K31/7048
Inventor 邹敬韬华楠杨振中赵莜萍
Owner TONGHUA HUAXIA PHARMA
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