The invention discloses an anti-fatigue energy composition which is prepared by the following raw materials in parts by weight: expanded black rice flour, oatmeal, coconut oil, corn syrup, soybean protein isolate powder, maltodextrin, carnitine, vine tea flavone extract, grape seed extract, quercetin extract, octacosanol, caffeine, branched chain amino acid, taurine, low fat cocoa and concentrated vanilla; the anti-fatigue energy composition is capable of rapidly supplementing sugar, fat, protein, electrolyte and other nutrients which are needed by the organism and promoting the nutrients to be absorbed, can be used for rapidly supplementing the consumed carbohydrate and the lost nutrients of human body caused by sweating excessively under the condition of exercises, especially medium and high intensity exercises and exercise training, and is beneficial for relieving central and peripheral fatigue; after the anti-fatigue energy composition is adopted, the antioxidant capacity and the aerobic metabolism level of the organism are improved, the oxygen utilization rate of cells can be improved, the moment and fatigue resistance of the organism can be better rapidly improved, and the sport injury is avoided.