29 results about "Gnaphalium affine" patented technology

The invention provides a probiotic composition for improving immunity and a preparation method thereof, and belongs to the technical field of probiotics. The preparation method comprises the following steps: fermenting selenium-enriched nostoc sphaeroides through selenium-enriched saccharomycetes to extract selenium-enriched nostoc sphaeroides polysaccharide and selenium-enriched nostoc sphaeroides protein peptide, mixing with traditional Chinese medicine polysaccharide obtained by extracting astragalus membranaceus, rhodiola rosea, codonopsis pilosula and pseudo-ginseng, and reacting with ferric salt to prepare a polysaccharide-Fe / selenium compound; solid residues are mixed and fermented, and active protein peptide is prepared from the solid residues and selenium-rich nostoc sphaeroides protein peptide; the probiotic composition capable of improving immunity is prepared by extracting a polysaccharide-Fe / selenium compound and a polysaccharide-Fe / selenium compound through an organic solvent, extracting a permeate through an organic solvent to obtain an active component, collecting a bacterial liquid, mixing and embedding the bacterial liquid in the polysaccharide-Fe / selenium compound and sodium alginate, and uniformly mixing the bacterial liquid with the active protein peptide, the active component, taurine and ginsenoside Rg3, so that the probiotic composition capable of improving immunity can play good anti-inflammatory, anti-oxidation, immunoregulation, anti-tumor, anti-virus and other roles, and has a good application prospect. Wide application prospects are realized.

This invention discloses a method for preparing wild jujube (Gnaphalium affine) essential oil that cannot be chemically replicated, belonging to the field of natural plant essential oil extraction technology. The method includes whole-plant cleaning pretreatment, low-temperature slicing, low-temperature fine crushing, low-pressure fractional distillation, graded condensation collection of aromatic fractions, separation, low-temperature aging, and filtration steps. This invention, through low-temperature slicing and fine crushing pretreatment combined with low-pressure fractional distillation at 0.02–0.08 MPa and no higher than 45°C, along with gradient condensation and low-temperature aging at 2–8°C, maximizes the preservation of natural synergistic trace components in the wild jujube essential oil. In gas chromatography-mass spectrometry (GC-MS) analysis, the obtained essential oil exhibits a characteristic aroma component retention rate ≥90% and contains at least three undisclosed natural synergistic trace components, whose aroma and activity cannot be completely replicated through chemical flavoring. This invention's process is mild and efficient, suitable for large-scale production, and provides a high-value-added pathway for the deep processing of wild jujube.

This application provides an open tissue culture method for *Gnaphalium affine*, comprising the following steps: S1: sterilizing *Gnaphalium affine* explants to obtain pretreated explants; S2: inserting the pretreated explants into a first modified MS medium for a first-stage culture to obtain first-stage explants; S3: transferring the first-stage explants to a second modified MS medium for a second-stage culture to obtain second-stage explants; S4: transferring the second-stage explants to a third modified MS medium for a third-stage culture to obtain primary seedlings. By sequentially adding cell-free fermentation filtrate and PPM to the three-stage modified MS medium, efficient culture of *Gnaphalium affine* explants in an open environment is achieved, reducing contamination rate and browning degree, while promoting explant healing, adventitious bud differentiation, and root primordia formation, thus improving the seedling survival rate of open tissue culture of *Gnaphalium affine*.

The invention relates to the technical field of medicines, and discloses a preparation method and application of an alcohol extract of root tuber of pilose asiabell. The preparation method comprises the following steps: crushing dried tuberous roots of cayenne pepper, sieving, weighing cayenne pepper powder, adding 95% ethanol, soaking and extracting at room temperature for 2 days, stirring once within 2 hours, once stirring for 30 minutes, performing suction filtration after soaking to obtain ethanol leaching liquor, soaking and extracting filter residues for 2 times, merging the ethanol leaching liquor, transferring into a rotary vacuum concentrator, and concentrating to obtain the cayenne pepper pure extract. Concentrating and drying to obtain a maozhou root tuber alcohol extract; experimental verification shows that the prepared pure extract of the sauced head has the effect of relieving the fatty liver, so that the pure extract of the sauced head can be used for preparing the medicine for relieving the fatty liver.

This invention discloses a method for cultivating pearl grouper, belonging to the field of aquaculture technology. Through careful design of active component configuration and juvenile feeding according to size, component A includes the following raw materials: β-chitosan, oxalic acid, and *Gnaphalium affine* extract; component B includes the following raw materials: sugarcane extract, rye seed powder, and oryzanol; component C includes the following raw materials: fig polysaccharide, yeast β-glucan, and eucalyptus oil. When the juvenile size is 2.2~5.0cm, a basic feed supplemented with components A and B is provided; when the juvenile size is 5.1~10cm, a basic feed supplemented with components A and C is provided; when the juvenile size is greater than 10cm, a basic feed supplemented with components A, B, and C is provided. The cultivation method of this application can improve the resistance of pearl grouper to *Vibrio harveyi* infection and / or its low-temperature tolerance, and the cultivation method is simple and the raw materials are readily available.

This invention provides a traditional Chinese medicine compound for treating chronic pharyngitis, belonging to the field of biomedical technology. The invention comprises a self-formulated traditional Chinese medicine compound consisting of *Pearl Umbrella*, *Trichosanthes kirilowii*, *Sapindus mukorossi*, *Lycoperdon perlatum*, *Panax japonicus*, *Gnaphalium affine*, *Aster tataricus*, and prepared licorice root for the treatment of chronic pharyngitis. Addressing the complex pathogenesis of chronic pharyngitis, characterized by a mixture of deficiency and excess, phlegm and blood stasis, and yin deficiency, the ingredients in the compound are rationally combined and work synergistically to achieve the effects of promoting blood circulation without depleting yin, clearing heat without harming yang, resolving phlegm without hindering the spleen, nourishing yin without being cloying, combining tonification and purgation, using both warming and cooling methods, and treating both the root cause and the symptoms. This compound is suitable for the treatment and management of chronic pharyngitis.

The invention discloses a saussurea involucrata polysaccharide, a nanoemulsion thereof, and a preparation method and application of the saussurea involucrata polysaccharide. The specific method comprises the following steps: firstly, drying and grinding saussurea involucrata peel into powder; selecting bacillus altitudinis cultured to the second generation as a fermentation strain, carrying out inoculation fermentation treatment on the powder, and filtering to obtain supernate; and adding ethanol for alcohol precipitation overnight, centrifuging, performing rotary evaporation, removing protein by a Sevage method, dialyzing, purifying, freeze-drying, and repeating the operation to obtain the product. According to the present invention, the Prunus persica pericarp polysaccharide is extracted and purified, the standardized process is established, the Prunus persica polysaccharide nano-emulsion is prepared, and the high encapsulation efficiency and the stability are achieved by optimizing the surfactant system; according to the invention, the saussurea nipponica polysaccharide and the nanoemulsion thereof are innovatively applied to shrimp coating preservation, and a wax layer barrier is broken through by utilizing the nano-size effect and the interface adaptability of the saussurea nipponica polysaccharide, so that microbial proliferation can be remarkably inhibited, fat oxidation can be reduced, the water binding capacity and sensory quality can be maintained, and the shelf life can be effectively prolonged.

The invention discloses a stripe rust resistance gene YrRC1 derived from roegneria ciliata as well as an encoding protein and application thereof. The stripe rust resistance gene YrRc1 provided by the invention is derived from a roegneria ciliata 2Sc chromosome, the nucleotide sequence of the gene is SEQ ID NO.1, and the amino acid sequence of the gene is SEQ ID NO.2. The invention further discloses a preparation method of the stripe rust resistance gene YrRc1. The invention also protects the application of the YrRC1 in improving the disease resistance of plants. The YrRc1 gene is induced by puccinia striiformis to realize rapid up-regulation expression; a VIGS system is utilized to silence YrRc1 in a disease-resistant material DA2Sc to enhance the plant susceptibility; the YrRc1 gene is introduced into a stripe rust susceptible variety Fielder through a transgenic technology, so that the stripe rust resistance can be remarkably improved. Therefore, the YrRc1 can positively regulate and control the stripe rust resistance and has important significance on wheat disease-resistant breeding.

This invention discloses a simple and rapid breeding method for artificially synthesizing wheat. It involves inducing incomplete meiosis in *Gnaphalium affine*, a close relative of wheat, under high red light (R / FR≥1.4) to produce unmeiotic divisions. This method falls under the field of crop breeding technology and includes the following steps: *Gnaphalium affine* (DD) cultured in a high red light (R / FR≥1.4) environment undergoes incomplete meiosis during reproductive growth to produce unmeiotic gametes. These gametes are then used as the male parent and crossed with *Langdon* (AABB). The resulting F1 generation (ABDD) is tetraploid. The F2 generation obtained by self-pollination of this F1 generation is hexaploid (AABBDD). Furthermore, the artificially synthesized hexaploid wheat offspring can be stably inherited.

This invention discloses a high SOD activity *Gnaphalium affine* juice, a *Gnaphalium affine* juice probiotic fermented product, and its preparation method. Using *Gnaphalium affine* as raw material, this invention obtains a high SOD activity *Gnaphalium affine* juice through a combination of repeated low-temperature treatment, pulping, ultrasonic crushing pretreatment, and heat treatment. Fermentation is carried out using *Lactobacillus mucilaginosus* Grx07, with the addition of a commercial fermenting agent to regulate the fermentation process. This achieves a synergistic effect between probiotics and *Gnaphalium affine* juice in terms of SOD activity, improving the SOD activity, viable cell count, and sensory quality of the fermented product. It has broad application prospects in the preparation of probiotic fermented foods with high antioxidant activity.

This invention relates to the field of veterinary medicine technology and provides a traditional Chinese medicine composition for treating coccidiosis in chickens, along with its preparation method. The composition is prepared from a combination of raw materials including *Gnaphalium affine*, *Hedyotis diffusa*, *Dendrobium nobile*, *Hemiberlesia argyi*, and *Thalictrum sylvestris*. This pure traditional Chinese medicine preparation exhibits no drug resistance, no drug residues, no toxic side effects, and no palatability issues. It also possesses advantages such as significant efficacy, rational formulation, simple preparation, low cost, and environmental friendliness. The traditional Chinese medicine composition of this invention has the effects of stopping diarrhea and killing parasites, clearing heat and drying dampness, and tonifying the middle and replenishing qi. It has a significant effect against coccidiosis in chickens, effectively preventing abnormal feces, intestinal bleeding, and intestinal ulcers caused by coccidiosis, and rapidly restoring the production performance of chicken flocks, thus improving breeding profits.

The invention provides a microorganism for improving grassland and application of the microorganism for improving the grassland, the microorganism for improving the grassland comprises Bradyrhizobium japonicum TG101, and further comprises one or more of Brevibacterium tumefaciens TG15, Phyllobacterium tumefaciens TG47 and Enterobacter ludwigii DP20, and the microorganism for improving the grassland further comprises one or more of Bradyrhizobium japonicum TG101, the Brevibacterium tumefaciens TG15, the Phyllobacterium tumefaciens TG47 and the Enterobacter ludwigii DP20. According to the application, novel application of the brevibacterium friariorum TG15, the leaf bacillus dulcis TG47 and the enterobacter ludwigii DP20 is innovatively found, that is, the three bacteria and the combination thereof can promote nodulation of the bradyrhizobium elwardii TG101; the compound microbial fertilizer disclosed by the invention has the functions of greatly improving the nodulation quantity, root nodule diameter and nitrogen fixation capability of lespedeza daurica in the field, and greatly improving the yield, quality and medicinal components of lespedeza daurica.

This invention discloses the application of dermal ethylsin in the preparation of antibacterial and repairing cosmetics or pharmaceuticals, and compositions containing it. Dermal ethylsin can inhibit Propionibacterium acnes, Staphylococcus aureus, and / or Staphylococcus epidermidis, and can promote keratinocyte migration and / or upregulate the expression of repair-related genes. Cytotoxicity tests show that dermal ethylsin has good safety. Human efficacy evaluation shows that serums containing dermal ethylsin can significantly reduce inflammatory papules, improve skin redness, and improve barrier function. Therefore, dermal ethylsin can be used as a dual-action antibacterial and repairing active agent in cosmetics or pharmaceuticals for acne skin care, sensitive skin repair, and / or post-medical aesthetic repair. Dermal ethylsin is derived from *Gnaphalium affine*, has good safety, and is non-irritating to the skin.

This invention discloses a method for correcting seed testing data of *Gnaphalium affine* based on transfer learning, belonging to the field of seed quality testing technology. This method collects benchmark data from standard equipment under standard conditions and target data from different testing equipment and environmental conditions to construct an equipment-environment error database and fit an error influence function. The benchmark data is set as the source domain, and the target data as the target domain. A transfer correction model is trained to correct the target domain data, and the classification threshold is dynamically updated. The corrected data is then mapped to a unified feature space to verify consistency, ultimately outputting standardized results and data traceability information applicable across laboratories. This method effectively eliminates systematic errors, standardizes testing data, improves correction accuracy and data reusability, ensures result reliability and traceability, and provides technical support for the standardization of seed testing.

The present application relates to the technical field of external medicine for treating urticaria, and discloses a composition for treating urticaria, which comprises the following components: Cudrania tricuspidata, Eucalyptus globulus, Cymbopogon citratus, Datura stramonium, Kaempferia rotunda, Sageretia theezans, Gleditsia sinensis, camphor, Gnaphalium affine and Gynura japonica. The composition is externally applied to the skin of the affected part, and has the effects of clearing heat and detoxifying, removing dampness and swelling, dispelling wind and relieving itching.

This invention relates to a traditional Chinese medicine composition for treating sleep disorders and its preparation method. The composition, by weight, is prepared from the following raw materials: 15-30 parts calcined amethyst, 10-20 parts Albizia bark, 5-15 parts *Gnaphalium affine*, 5-15 parts silkworm cocoon, 10-30 parts yam, 5-10 parts *Bupleurum chinense*, 15-30 parts wheat bran, 2-4 parts *Panax notoginseng* powder, 10-20 parts *Adenophora stricta*, 3-6 parts clove, and 3-6 parts nutmeg. Based on the principle of combining disease and syndrome differentiation in traditional Chinese medicine, this invention selects natural drugs with heart-nourishing and calming effects, and formulates them according to a specific weight ratio. This provides a traditional Chinese medicine composition for treating sleep disorders that is highly effective and low-cost. Simultaneously, it provides a corresponding preparation method that is simple, easy to implement, and readily produced, maximizing the extraction of the effective components of each drug element, and has good market application prospects.

A consumable is provided. The consumable includes (a) at least one sweetener, and (b) a sweetness modifier comprising an extract or fraction from Gnaphalium affine or at least one flavonoid compound. The at least one sweetener is present is a sweetening amount.

According to the device, a conveyor belt is arranged on a machine body in a penetrating mode, a partition plate is arranged on the upper surface of the conveyor belt, a vibration motor is arranged on the left side of the machine body, the output end of the vibration motor is fixedly connected with a flow guide frame, the flow guide frame is located above the partition plate, and an electric telescopic rod is fixedly connected into the machine body. An electric telescopic rod is arranged on the machine body, a C-shaped plate is fixedly connected to the output end of the electric telescopic rod, a sponge block is arranged on the lower surface of the C-shaped plate, a cleaning assembly and a screening assembly are arranged on the machine body, and two robots are arranged in the machine body and located on the two sides of the conveying belt correspondingly. The sedum aizoon is cleaned to remove soil and then screened, so that the appearance is clearer, screening is facilitated, improvement of subsequent seedling raising and transplanting uniformity is facilitated, meanwhile, the sedum aizoon can be limited, and displacement during cleaning is avoided.

This invention relates to the field of biotechnology, specifically to a PiRNA primer set for detecting *Gnaphalium affine* infection in Italian honeybees, a kit for the same, and its application. The primer set includes the following primers: piR-1182394-F, piR-1182394-R, U6-F, and U6-R. The nucleotide sequence of piR-1182394-F is shown in SEQ ID No. 1; the nucleotide sequence of piR-1182394-R is shown in SEQ ID No. 2; the nucleotide sequence of U6-F is shown in SEQ ID No. 3; and the nucleotide sequence of U6-F is shown in SEQ ID No. 4. This study discovered and identified a specific PiRNA molecule, piR-1182394, whose expression changed significantly after *Gnaphalium affine* infection of Italian honeybee worker larvae. The primer set provided in this study can be used for the early diagnosis of chalkbrood in Italian honeybees, with a diagnostic time earlier than traditional methods. It can provide a diagnostic basis for whether bee colony larvae are infected with chalkbrood before the larvae develop symptoms, providing a new perspective and target for elucidating the molecular mechanism of Italian honeybee resistance to chalkbrood, and laying a theoretical foundation for the future development of novel bee disease control technologies based on RNA interference.

A preparation method of the active part comprises the following steps: taking dry bergenia scopulosa rhizome, crushing, sieving to obtain powder, weighing a certain amount of powder, adding a methanol solution with the volume fraction of 80%, carrying out ultrasonic extraction, centrifuging, and collecting supernate, so as to obtain the active part. Concentrating the supernatant to 1 / 2-1 / 3 volume, carrying out D101 type macroporous adsorption resin column chromatography, eluting with distilled water, a 30% ethanol solution, a 50% ethanol solution and a 70% ethanol solution in sequence, eluting with two column volumes by each eluent, collecting fractions obtained by different eluents, and carrying out vacuum concentration and drying to obtain components F1, F2, F3 and F4 in sequence; wherein the component F3 is the active site.

This invention provides a lung-clearing and cough-relieving herbal remedy, which, based on the total weight percentage of the dried plant materials used to prepare the remedy, comprises the following main components: 8-10% *Corydalis yanhusuo*, 8-10% *Gnaphalium affine*, 8-10% *Citrus reticulata* peel, 7-10% *Houttuynia cordata*, 7-10% *Trichosanthes kirilowii*, 7-9% *Houttuynia cordata*, 7-9% *Polygonum cuspidatum*, 7-9% *Sarcandra glabra*, 6-8% *Adenophora stricta*, 6-8% *Fritillaria cirrhosa*, and 5-7% *Perilla frutescens*. This invention utilizes 11 medicinal herbs... Among the list of items that are both food and medicine, this product is precisely formulated according to the theories of "four qi and five flavors" and meridian tropism. It is rich in natural active ingredients such as fritillary alkaloids, fritillary alkaloid A and B, hesperidin, and houttuynia cordata. It has both dietary and medicinal properties, and is extremely safe. In the first stage, it targets and detoxifies, clears blood vessels and eliminates the root cause of the disease. In the second stage, it reduces inflammation and kills bacteria, repairs damaged alveoli and promotes regeneration. This progressive treatment can not only dissipate pulmonary ground-glass nodules and solid nodules, but also inhibit density transformation and prevent recurrence.

This invention discloses a method for intercropping multiple forage grasses in citrus orchards. Addressing the drawbacks of traditional clean cultivation management and single-grass intercropping in citrus orchards, it employs a mixed sowing of four varieties: ryegrass, white clover, gnaphalium affine, and milkvetch. It includes standardized steps for orchard pretreatment, seed pretreatment, precision sowing, seedling management, growth period management, forage harvesting and returning to the field, and water and fertilizer regulation. Strict control of parameters at each stage is maintained, and a specific ratio of compound bio-fertilizer is selected to optimize the citrus water and fertilizer management scheme. This invention achieves year-round closed coverage of the citrus orchard through the complementary characteristics of the four forage grasses, improving the control rate of noxious weeds, reducing soil erosion, increasing soil organic matter content, and increasing citrus yield per acre. It combines the advantages of improving soil fertility, suppressing weeds and conserving moisture, improving quality and efficiency, and promoting a green and ecological environment, thus constructing a highly efficient and sustainable citrus ecological planting system.

This invention provides the application of norzelamin in the preparation of NDM-1 enzyme inhibitors. Through cefotaxime hydrolysis assays, checkerboard minimum inhibitory concentration (MIC) tests, growth curves, bactericidal curves, hemolysis tests, and *Gnaphalium affine* infection experiments, it was demonstrated that norzelamin can inhibit NDM-1 enzyme activity and exhibits good synergistic antibacterial activity with the carbapenem antibiotic meropenem, enhancing the protective effect of meropenem against *Gnaphalium affine* larval infection models. Therefore, norzelamin, as a potential adjuvant for β-lactam antibiotics, can enhance the antibacterial effect of β-lactam antibiotics by inhibiting NDM-1 enzyme activity, which is of great significance for the clinical development of safe and effective β-lactam antibiotic adjuvants.

This invention provides a method for rapidly cultivating P5 shrimp larvae, comprising the following steps: transferring nauplii into a larval rearing pond for cultivation; after more than 80% of the nauplii have developed into zoea larvae, feeding them artificial feed for zoea larvae; after more than 80% of the zoea larvae have developed into mysid larvae, feeding them feed for Artemia nauplii and mysid larvae; after more than 80% of the mysid larvae have metamorphosed into shrimp larvae, feeding them rotifers and shrimp flakes; the artificial feed for zoea larvae includes: 45-55 parts of Schizochytrium powder, 15-27 parts of soy milk, 10-15 parts of egg yolk, and 15-20 parts of *Gnaphalium affine* oil. The artificial feed for mysid larvae includes: 40-44 parts of shrimp flakes, 12-16 parts of tubifex worm powder, 5-10 parts of mannan oligosaccharide, 10-14 parts of scabra oil, and 20-30 parts of *Eupatorium fortunei* oil. This invention shortens the development time while obtaining high-quality P5 shrimp larvae, and throughout the entire cultivation process, the shrimp larvae mortality rate is low and the seedling survival rate is high.

This utility model discloses a seed sowing and positioning device for *Hedysarum heterotropoides*, which includes a horizontal base frame. X-axis guide rails are fixedly connected to the inner walls of both sides of the horizontal base frame. A first bidirectional motor is fixedly connected to one end of each X-axis guide rail. The output end of the first bidirectional motor is threadedly connected to an X-axis slider via an X-axis lead screw. The X-axis slider is fixedly connected to a Y-axis guide rail. A second bidirectional motor is fixedly connected to one end of each Y-axis guide rail. The output end of the second bidirectional motor is threadedly connected to a Y-axis slider via a Y-axis lead screw. The device comprises a seed box, a rectangular through-hole, a mounting shell, batch feeding wheels, a stepper motor, a drive shaft, and a feeding hole, forming a flexibly controllable batch sowing mechanism. This prevents the amount of seeds sown at the sowing position from being too little or too much, making the sowing process more stable.

This application relates to the field of plant cultivation technology, and in particular to a method for establishing a high-frequency regeneration system for *Gnaphalium affine*. The method includes: S1, explant preparation and disinfection: *Gnaphalium affine* seeds are disinfected and inoculated onto a primary culture medium under aseptic conditions to obtain sterile seedlings; S2, shoot induction: the terminal buds of the sterile seedlings obtained in step S1 are cut as explants and inoculated into a first induction culture medium to induce the generation of shoot clusters; S3, shoot proliferation: the shoot clusters obtained in step S2 are divided and transferred to a second induction culture medium to promote the proliferation and growth of the shoot clusters; S4, rooting culture: the robust shoot clusters obtained in step S3 are transferred to a rooting culture medium to induce the formation of adventitious roots; S5, hardening and transplanting: the tissue culture seedlings with complete root development from step S4 are hardened and then transplanted into a cultivation substrate to obtain regenerated *Gnaphalium affine* plants. This application establishes a rapid propagation system for *Gnaphalium affine*.

The invention discloses leg essence containing rutin, the leg essence is a double-phase essence, and the leg essence is composed of an oil phase and a water phase according to a weight ratio of (1-4): (2-7), the water phase is prepared from the following components in parts by weight: 0.01 to 1.5 parts of rutin liposome, 0.1 to 5 parts of grape vine extract, 0.1 to 3 parts of microcirculation dynamic factors, 0.1 to 4 parts of jania rubens extract, 0.5 to 1.5 parts of hexanediol, 0.5 to 0.8 part of p-hydroxyacetophenone, 1 to 8 parts of 1, 2-pentanediol, 0.2 to 1 part of thickening agent and the balance of water; the oil phase is prepared from the following components in parts by weight: 0.1 to 6 parts of abelmoschus manihot essential oil, 1 to 10 parts of plukenetia volubilis seed oil, 1 to 10 parts of pumpkin seed oil, 0.1 to 5 parts of zingiber zingiberensis extract, 0.1 to 3 parts of grapefruit essential oil, 20 to 50 parts of coconut oil alcohol-octoate / caprate, 20 to 40 parts of isopropyl myristate and 10 to 30 parts of squalane. The components in the formula supplement each other and have a synergistic effect, the conditions of leg edema and obesity are effectively improved, the water storage capacity of the skin is promoted, blood circulation and metabolism are promoted, and the oil is not greasy.

The invention provides a traditional Chinese medicine composition for inhibiting osteosarcoma progress by activating macrophages, and relates to the technical field of traditional Chinese medicine preparations and immunoregulation. The traditional Chinese medicine composition for inhibiting osteosarcoma progress by activating macrophages is prepared from the following traditional Chinese medicinal materials in parts by weight: 20 to 40 parts of radix astragali seu hedysari, 5 to 15 parts of bulbus fritillariae thunbergii, 20 to 40 parts of semen coicis, 20 to 40 parts of herba hedyotidis diffusae, 4 to 8 parts of radix platycodonis, 10 to 20 parts of herba salviae chinensis, 10 to 20 parts of radix cudramiae, 10 to 20 parts of pumice and auxiliary medicinal materials. According to the invention, macrophages are induced to be polarized to M1 type, so that the phagocytic ability and cytokine release of the macrophages are enhanced, and tumor cells are killed and inhibited. In-vitro and in-vivo experiments show that the traditional Chinese medicine composition can significantly enhance the anti-tumor activity of macrophages and inhibit proliferation, migration and metastasis of tumor cells, so that a safe and effective traditional Chinese medicine scheme is provided for tumor immunotherapy.

This application provides a composite microbial community and a method for copper extraction by bioleaching assisted by a coupled electric field. The composite microbial community includes *Thiobacillus ferrooxidans*, *Aspergillus niger*, and *Gnaphalium affine*. The composite microbial community is obtained by inoculating *Thiobacillus ferrooxidans*, *Aspergillus niger*, and *Gnaphalium affine* in a composite culture medium at an inoculation ratio of 2:(0.5~2):(0.5~2) and co-culturing them. Under the assistance of an electric field, the composite microbial community synergistically promotes the leaching and extraction of copper from copper-containing substances. This method, through the coupling and coordination of biological and electric field effects, improves the copper leaching and recovery efficiency, and has the advantages of mild conditions, environmental friendliness, and high resource utilization efficiency.