8results about How to "Improve intake efficiency" patented technology

This invention provides a fluorinated DSPE-PEG material, its preparation method, and its applications, belonging to the fields of nanomedicine delivery systems and biomedicine. This invention prepares fluorinated DSPE-PEG materials with different fluorination modification rates by fluorinating distearate phosphatidylethanolamine polyethylene glycol amino groups, and uses these materials as carriers to load sangxin (a type of glycerol) to form fluorinated nanomicelles. This invention systematically screens the effect of fluorination modification rate on the performance of the nanomicelles, determines the optimal fluorination modification rate, and confirms that it can overcome the defect of low cellular uptake of PEGylated carriers and enhance the anti-colorectal cancer activity of the loaded drugs. The fluorinated nanomicelles have uniform particle size, good stability, and excellent biocompatibility with the carrier material. This invention provides a new strategy for the efficient delivery of small molecule active ingredients from traditional Chinese medicine and the treatment of colorectal cancer.

The invention relates to lysosome-based nano drug-loaded particles and application thereof, and belongs to the technical field of biological medicines. According to the invention, the lysosome membrane is used as a biomimetic membrane source to coat the surface of the drug nanoparticle, so that the structure stability of the lysosome membrane is maintained, and the activity of hydrolase which naturally exists is retained to the greatest extent, so that the drug-loaded nanoparticle can perform in-situ degradation on an extracellular matrix of a tumor tissue in an in-vivo delivery process; therefore, the physical barrier of the tumor tissue is effectively weakened, the permeation, distribution and cellular uptake ability of the nano-drug in the tumor tissue are remarkably enhanced, the condition that immune cells enter the tumor tissue is improved on the basis, and finally the tumor treatment effect is improved.

The application belongs to the technical field of biological medicine and molecular biology, and particularly relates to a CAR-macrophage with enhanced efferocytosis targeting apoptotic cells and a preparation method and application thereof. The CAR provided by the application comprises a signal peptide segment, a ligand recognition domain, a tag gene, a hinge region, a transmembrane region and an intracellular signal domain, can recognize lipid or protein signals exposed on the surface of apoptotic cells in an inflammatory microenvironment, and realizes targeted phagocytosis and aggregation in an inflammatory area. DKP type unsaturated ionizable lipids have protonation characteristics in an acidic microenvironment, which helps mRNA encapsulation and endosome escape. Lipid nanoparticles contain CAR mRNA and can respond to broken surface ligands. Under inflammatory conditions, the ligands fall off to expose DOPS, thereby improving the endocytosis capacity of macrophages. The CAR-macrophage and the nanoparticles can be used to prepare drugs for treating diseases such as metabolic-associated fatty liver disease and atherosclerosis, realize multiple synergies, have high specificity and safety, and have good industrialization prospects.

This invention relates to the field of biomedical nanomaterials and pharmaceutical formulations, and discloses a biomimetic nanoenzyme drug for treating gouty arthritis, its preparation method, and its application. The biomimetic nanoenzyme drug is a core-shell structured nanoparticle, comprising, from the innermost layer to the outermost layer: ① a catalytic and antioxidant core, formed by a composite of carbon dots and manganese dioxide; ② a molecularly imprinted polymer layer coating the core, the molecularly imprinted polymer being formed using uric acid as a template molecule; and ③ a platelet cell membrane layer coating the molecularly imprinted polymer layer. This invention utilizes the aforementioned biomimetic nanoenzyme drug for treating gouty arthritis, its preparation method, and its application. This drug, through a multi-level biomimetic assembly strategy, integrates carbon dots (CDs) to enhance antioxidant activity, molecularly imprinted polymers (MIPs) to impart uric acid selectivity, and platelet membrane (PLM) camouflage to improve targeting and compatibility, thereby achieving a safe and highly effective synergistic treatment for gouty arthritis.

This invention belongs to the field of pharmaceutical preparation technology, specifically relating to a hyaluronic acid-modified co-supported nanoparticle, its preparation method, and its application. The nanoparticle has a core-shell structure, with the core being a coordination complex of zinc-doped Prussian blue and sinomenine hydrochloride, and the outer shell being a bovine serum albumin-hyaluronic acid conjugate. This invention improves the therapeutic effect on knee osteoarthritis.

The invention relates to the field of plant external vesicles, in particular to a ginseng external vesicle composition loaded with quercetin as well as a preparation method and application thereof, and the preparation method comprises the following steps: preparing ginseng external vesicle supernate; the preparation method comprises the following steps: dissolving quercetin in dimethyl sulfoxide to obtain a quercetin solution; mixing the quercetin solution with the ginseng outer vesicle supernate, and incubating at room temperature for 1-2 hours; and centrifuging and carrying out solid-liquid separation to obtain a precipitate, namely the composition. The quercetin is loaded by using the extracellular vesicles, so that ROS generation can be reduced, the activity of SOD in cells can be improved, and the MDA content can be reduced. The activity of key enzyme in a cell anti-oxidation system is enhanced, so that damage caused by oxidative stress is relieved, and a certain repairing effect is achieved.

This invention belongs to the field of biomedical technology, specifically relating to a dihydroporphyrin e6 conjugate and its applications. Using dihydroporphyrin e6 and N-p-toluenesulfonyl ethylenediamine as raw materials, an N-p-toluenesulfonyl ethylenediamine-dihydroporphyrin e6 conjugate is prepared by condensation; then, the N-p-toluenesulfonyl ethylenediamine-dihydroporphyrin e6 conjugate is condensed with excess galactosamine hydrochloride to prepare an N-p-toluenesulfonyl ethylenediamine-dihydroporphyrin e6-digalactosamine conjugate. This conjugate exhibits improved water solubility and can target liver cancer cells and the intracellular endoplasmic reticulum, showing potential application value in the field of photodynamic immunotherapy for liver cancer.

The application discloses a universal targeted delivery system suitable for multi-category functional compositions, a preparation method and application, and belongs to the technical field of active ingredient delivery. The application takes a targeted functional peptide combined with a hydroxypropyl-beta-cyclodextrin complex system as a core, and is matched with liposomes, exosomes, antibody coupling and stimulus-responsive delivery systems; the molecular weight of the targeted functional peptide is limited to 120Da-5000Da, and the particle size of the liposomes is limited to 20nm-200nm. The application can load twelve categories of functional active components and traditional Chinese medicine extracts, and is suitable for health foods, nutritional supplements, traditional Chinese medicine preparations and ordinary functional foods. The application optimizes carrier parameters and combination modes through tests, is not a simple superposition of prior art, has strong global adaptability, a simple process and high industrialization, can effectively improve the stability of active ingredients and the targeted delivery effect, has high technical barriers, and has high application and commercial values.