21results about How to "Improve intake efficiency" patented technology

The invention discloses a GSH response type cell membrane bionic nano-drug, a preparation method and application, the cell membrane bionic nano-drug comprises cell membrane vesicles serving as a carrier, the cell membrane vesicles are prepared by performing cell membrane extraction and membrane extrusion on immune cells of which the membrane surfaces overexpress azide, and the cell membrane vesicles serve as a carrier. The azide on the cell membrane vesicles and the bio-coupling compound are bonded through a click chemical reaction to form-SH groups, and the-SH groups and the small-molecule inhibitor are combined together in a disulfide bond mode through the cross-linking agent. The drug is connected with a small-molecule inhibitor through a disulfide bond, so that the drug loading rate is relatively higher, the stability is better, and drug leakage in the blood circulation process is relatively less; the small-molecule inhibitor can be completely released only in a tumor site microenvironment, so that the tumor m6A methylation level is reduced, the EMT state is reversed, the tumor drug resistance is overcome, and a better tumor treatment effect is displayed in cooperation with chemotherapy drugs for tumor treatment.

The invention discloses low-GI nutritional meal replacement powder and a preparation method thereof, and belongs to the technical field of preparation of low-GI meal replacement powder. The meal replacement powder is prepared from the following components in parts by weight: 10-20 parts of whey protein powder, 5-10 parts of konjaku flour, 3-5 parts of a roxburgh rose and cactus polysaccharide / sacha inchi protein compound, 3-5 parts of quinoa flour, 3-5 parts of highland barley flour, 3-5 parts of chickpea flour, 3-5 parts of corn flour, 3-5 parts of coarse rice flour, 1-3 parts of citrus fibers, 1-3 parts of pumpkin fibers, 1-3 parts of inulin and 1-3 parts of a hawthorn extract. 1 to 3 parts of blueberry extract, 0.5 to 2 parts of DL-malic acid and 0.5 to 2 parts of isomaltulose. The roxburgh rose cactus polysaccharide, the sodium alginate and the sodium carboxymethyl cellulose jointly act on the sacha inchi protein, hydrogen bonds and Van der Waals force are formed, protein degradation is slowed down, the slow release effect is enhanced, the bioavailability is improved, and the sacha inchi protein contains multiple amino acids and can assist muscle recovery; the whey protein powder and the konjaku flour are utilized, so that the raw materials are uniformly dispersed, the granularity is refined, and the brewing performance and the digestion and absorption efficiency are improved.

The present invention provides a drug delivery system, specifically provides a pharmaceutical carrier lipid conjugate, a pharmaceutical composition containing the lipid conjugate, and its preparation and application, which relates to the field of drug delivery. The lipid conjugate proposed in the present invention can bind to tumor cell surface receptors to improve cell uptake efficiency through structural improvement of the lipid conjugate itself and targeting peptides on the conjugate, thereby improving the expression level.

This invention provides a fluorinated DSPE-PEG material, its preparation method, and its applications, belonging to the fields of nanomedicine delivery systems and biomedicine. This invention prepares fluorinated DSPE-PEG materials with different fluorination modification rates by fluorinating distearate phosphatidylethanolamine polyethylene glycol amino groups, and uses these materials as carriers to load sangxin (a type of glycerol) to form fluorinated nanomicelles. This invention systematically screens the effect of fluorination modification rate on the performance of the nanomicelles, determines the optimal fluorination modification rate, and confirms that it can overcome the defect of low cellular uptake of PEGylated carriers and enhance the anti-colorectal cancer activity of the loaded drugs. The fluorinated nanomicelles have uniform particle size, good stability, and excellent biocompatibility with the carrier material. This invention provides a new strategy for the efficient delivery of small molecule active ingredients from traditional Chinese medicine and the treatment of colorectal cancer.

The invention relates to lysosome-based nano drug-loaded particles and application thereof, and belongs to the technical field of biological medicines. According to the invention, the lysosome membrane is used as a biomimetic membrane source to coat the surface of the drug nanoparticle, so that the structure stability of the lysosome membrane is maintained, and the activity of hydrolase which naturally exists is retained to the greatest extent, so that the drug-loaded nanoparticle can perform in-situ degradation on an extracellular matrix of a tumor tissue in an in-vivo delivery process; therefore, the physical barrier of the tumor tissue is effectively weakened, the permeation, distribution and cellular uptake ability of the nano-drug in the tumor tissue are remarkably enhanced, the condition that immune cells enter the tumor tissue is improved on the basis, and finally the tumor treatment effect is improved.

The application belongs to the technical field of biological medicine and molecular biology, and particularly relates to a CAR-macrophage with enhanced efferocytosis targeting apoptotic cells and a preparation method and application thereof. The CAR provided by the application comprises a signal peptide segment, a ligand recognition domain, a tag gene, a hinge region, a transmembrane region and an intracellular signal domain, can recognize lipid or protein signals exposed on the surface of apoptotic cells in an inflammatory microenvironment, and realizes targeted phagocytosis and aggregation in an inflammatory area. DKP type unsaturated ionizable lipids have protonation characteristics in an acidic microenvironment, which helps mRNA encapsulation and endosome escape. Lipid nanoparticles contain CAR mRNA and can respond to broken surface ligands. Under inflammatory conditions, the ligands fall off to expose DOPS, thereby improving the endocytosis capacity of macrophages. The CAR-macrophage and the nanoparticles can be used to prepare drugs for treating diseases such as metabolic-associated fatty liver disease and atherosclerosis, realize multiple synergies, have high specificity and safety, and have good industrialization prospects.

The invention provides a milk exosome coated PLGA (poly (lactic-co-glycolic acid)) nano antituberculosis drug system as well as preparation and application thereof. The structure of the system is as follows: anti-tuberculosis drug-milk exosome-coated PLGA-anti-tuberculosis drug. The antituberculous drug is selected from one or a combination of more of aminoquinolones, benzothiazoles, aryl quinoline, nitrobenzamides and benzothiazinone antituberculous drugs. According to the nano antituberculosis drug system, the circulation time of nano particles in blood is prolonged, and the bioavailability of the drug is improved. The cellular uptake efficiency of the nanoparticles is enhanced, and the drug delivery effect is improved. The immunological rejection and the potential toxicity are reduced, and the treatment safety is improved.

This invention relates to the field of biomedical nanomaterials and pharmaceutical formulations, and discloses a biomimetic nanoenzyme drug for treating gouty arthritis, its preparation method, and its application. The biomimetic nanoenzyme drug is a core-shell structured nanoparticle, comprising, from the innermost layer to the outermost layer: ① a catalytic and antioxidant core, formed by a composite of carbon dots and manganese dioxide; ② a molecularly imprinted polymer layer coating the core, the molecularly imprinted polymer being formed using uric acid as a template molecule; and ③ a platelet cell membrane layer coating the molecularly imprinted polymer layer. This invention utilizes the aforementioned biomimetic nanoenzyme drug for treating gouty arthritis, its preparation method, and its application. This drug, through a multi-level biomimetic assembly strategy, integrates carbon dots (CDs) to enhance antioxidant activity, molecularly imprinted polymers (MIPs) to impart uric acid selectivity, and platelet membrane (PLM) camouflage to improve targeting and compatibility, thereby achieving a safe and highly effective synergistic treatment for gouty arthritis.

The invention provides an Anti-PD-L1 and Anti-HER2 double-antibody guide nano-drug system as well as a preparation method and application thereof, and belongs to the technical field of composite material preparation and biological medicine. Perovskite quantum dots with a wide photoluminescence spectrum are combined to the surface of accurately designed and prepared Gd-MOF to obtain a nano composite material, and then the nano composite material is used as a carrier to be covalently coupled with Anti-PD-L1 and Anti-HER2 antibodies and loaded with an anti-cancer drug to obtain a double-antibody guided nano drug system. The double-antibody guided nano-drug system provides a multi-mode imaging strategy for tumor diagnosis, exerts the treatment effect of combining multiple treatment methods in tumor combined treatment, and realizes the synergistic effect of chemical treatment, immunotherapy and photodynamic therapy; the invention can be applied to the development and clinical practice of tumor diagnosis technology and tumor treatment drugs.

The invention discloses a preparation method of an anticancer drug auxiliary agent with a slow release function. The preparation method comprises the following steps: S1, preparing mesoporous alumina; s2, preparation of pretreated mesoporous alumina; s3, preparation of organic mesoporous alumina; s4, preparing modified mesoporous alumina; and S5, preparing the anti-cancer drug auxiliary agent. According to the anti-cancer drug adjuvant with the slow release function, the multifunctional anti-cancer drug adjuvant is prepared through multi-step surface modification, the adjuvant has the excellent effects of a mesoporous structure, cyclodextrin inclusion, folic acid targeting, drug absorption promotion and the like, efficient loading and controllable release of drugs can be achieved, and the anti-cancer drug adjuvant with the slow release function can be used for preparing the anti-cancer drug adjuvant with the slow release function. The active targeting and drug absorption promoting capabilities are also realized, so that the treatment effect of the anti-cancer drug is improved, and the bioavailability of the anti-cancer drug is improved.

This invention belongs to the field of pharmaceutical preparation technology, specifically relating to a hyaluronic acid-modified co-supported nanoparticle, its preparation method, and its application. The nanoparticle has a core-shell structure, with the core being a coordination complex of zinc-doped Prussian blue and sinomenine hydrochloride, and the outer shell being a bovine serum albumin-hyaluronic acid conjugate. This invention improves the therapeutic effect on knee osteoarthritis.

The invention discloses a preparation method and application of a sulfonamide zwitterionic block polymer. The invention first synthesizes a novel sulfonamide zwitterionic compound MAC-PEG-SAs, and then uses a hydrophobic macromolecular initiator to initiate atom transfer radical polymerization of MAC-PEG-SAs to prepare a zwitterionic block polymer of sulfonamide. In the obtained polymer, PCL, PLA and PLGA are hydrophobic segments, P(MAC-PEG-SAs) is a hydrophilic segment, and the block polymer can encapsulate hydrophobic drug molecules in an aqueous solution to form drug-loaded nano-micelles. The drug-loaded nanoparticles formed by encapsulating hydrophobic drugs can be efficiently enriched in hepatocellular carcinoma or brain glioma tissues, and have strong application potential in the field of anti-tumor drug delivery.

The present invention discloses the application of extracellular vesicles derived from HL-60 cells in a drug delivery system for targeting tumors. After LPS and IFN-γ treatment, the expression levels of related pro-inflammatory factors in the extracellular vesicles obtained in neutrophils were significantly upregulated, especially ICAM-1, an important indicator of neutrophil activation, was significantly increased at both mRNA and protein expression levels. Neutrophil-derived EVs with high expression of ICAM-1 are more easily adhered and captured by tumor cells with high expression of integrins. Drug-loaded EVs are prepared by loading Dox with neutrophil-derived EVs, which effectively improves the efficiency of drug-loaded EVs in vivo being taken up by tumor cells and their ability to kill tumor cells.

The invention discloses a hybrid bionic RBCM / Zn-MOF nano-drug delivery carrier and a preparation method and application thereof, and relates to the technical field of bionic nano-drug delivery, the nano-drug delivery carrier is FA-RBCM-CuS (at) ZIF-8, and the process comprises the following steps: synthesizing PVP-CuS NPs, mixing 2-methylimidazole and PVP-CuS NPs, adding zinc nitrate hexahydrate, and preparing CuS (at) ZIF-8; the preparation method comprises the following steps: connecting FA to the surface of RBCM to obtain FA-RBCM, mixing the FA-RBCM with CuS (at) ZIF-8, and extruding to obtain the FA-RBCM-CuS (at) ZIF-8 nano-drug delivery carrier. The obtained sample is uniform in particle size distribution and stable in physicochemical property. The carrier is high in DOX encapsulation efficiency and loading capacity, has responsive drug release capacity in a simulated tumor microenvironment, and is good in biological safety.

The invention relates to the field of plant external vesicles, in particular to a ginseng external vesicle composition loaded with quercetin as well as a preparation method and application thereof, and the preparation method comprises the following steps: preparing ginseng external vesicle supernate; the preparation method comprises the following steps: dissolving quercetin in dimethyl sulfoxide to obtain a quercetin solution; mixing the quercetin solution with the ginseng outer vesicle supernate, and incubating at room temperature for 1-2 hours; and centrifuging and carrying out solid-liquid separation to obtain a precipitate, namely the composition. The quercetin is loaded by using the extracellular vesicles, so that ROS generation can be reduced, the activity of SOD in cells can be improved, and the MDA content can be reduced. The activity of key enzyme in a cell anti-oxidation system is enhanced, so that damage caused by oxidative stress is relieved, and a certain repairing effect is achieved.

This invention belongs to the field of biomedical technology, specifically relating to a dihydroporphyrin e6 conjugate and its applications. Using dihydroporphyrin e6 and N-p-toluenesulfonyl ethylenediamine as raw materials, an N-p-toluenesulfonyl ethylenediamine-dihydroporphyrin e6 conjugate is prepared by condensation; then, the N-p-toluenesulfonyl ethylenediamine-dihydroporphyrin e6 conjugate is condensed with excess galactosamine hydrochloride to prepare an N-p-toluenesulfonyl ethylenediamine-dihydroporphyrin e6-digalactosamine conjugate. This conjugate exhibits improved water solubility and can target liver cancer cells and the intracellular endoplasmic reticulum, showing potential application value in the field of photodynamic immunotherapy for liver cancer.

The present invention relates to the technical field of intelligent nutrition management, and in particular to a method for optimizing dietary energy intake for obese people, the method comprising: determining nutritional characteristic values ​​of various foods according to a nutrient content sequence and performing a sorting operation to obtain a main nutrient data string of the various foods; analyzing each main nutrient data string and performing a corresponding first-class energy labeling; determining the energy density level and expansion level of various foods and performing a corresponding second-class energy labeling; based on the first-class energy labeling result and the second-class energy labeling result, correcting a primary energy sequence and adding a label stamp to obtain an actual energy sequence table; periodically obtaining parameters of a person to be optimized, inputting the parameters of the person to be optimized into a linear regression model to output an actual progress direction; and combining the actual energy sequence table, the first-class energy labeling result and the second-class energy labeling result according to the actual progress direction to output a dietary energy prediction result.

The invention belongs to the technical field of preparation of anti-tumor drugs, and particularly relates to ginger-sourced drug-loaded microparticles for targeting colorectal cancer and application of the ginger-sourced drug-loaded microparticles. The ginger-sourced drug-loaded microparticles for targeting colorectal cancer provided by the invention comprise ginger-sourced microparticles and drugs which are wrapped by the ginger-sourced microparticles and can inhibit colorectal cancer, and the surfaces of the ginger-sourced microparticles are also modified with transferrin binding ligands. The ginger-sourced drug-loaded microparticles can target colorectal tumor tissues and penetrate into the deep part of the tumor through oral delivery, so that the ginger-sourced drug-loaded microparticles are efficiently taken by colorectal cancer tumor cells and tumor stem cells. In addition, the ginger-sourced drug-loaded microparticles can also cooperate with drugs to inversely polarize tumor-associated macrophages into M1 type, improve the tumor microenvironment, enhance the drug sensitivity of colorectal cancer tumor cells, effectively kill colorectal cancer tumor cells and tumor stem cells, and achieve efficient inhibition of colorectal cancer.

The invention discloses a functional food for reducing fat and losing weight and a preparation method thereof. Comprising the following components in parts by weight: 30 to 50 parts of limonin-rutin-isoquercitrin-folic acid nanoparticles, 20 to 40 parts of fucoxanthin-glycyrrhizic acid-isoquercitrin-mannose nanoparticles, 0.1 to 0.5 part of vitamin D3-linoleic acid-isoquercitrin-hyaluronic acid nanoparticles, and the balance of water or solid filler auxiliary materials. The surfaces of limonin, fucoxanthin and vitamin D3 nanoparticles are respectively modified by folic acid, mannose and hyaluronic acid, and a biological enhancer and isoquercitrin are introduced, so that efficient intestinal absorption of weight-losing components such as limonin, fucoxanthin and vitamin D3 is promoted, meanwhile, the components are subjected to formula design, and the weight-losing effect is improved. The fat-reducing and weight-losing effects of the formula product are greatly improved, the fat accumulation is effectively reduced, and the body weight is reduced; the product can be prepared into capsules, tablets, electuary, oral liquid or beverage.

The application discloses a universal targeted delivery system suitable for multi-category functional compositions, a preparation method and application, and belongs to the technical field of active ingredient delivery. The application takes a targeted functional peptide combined with a hydroxypropyl-beta-cyclodextrin complex system as a core, and is matched with liposomes, exosomes, antibody coupling and stimulus-responsive delivery systems; the molecular weight of the targeted functional peptide is limited to 120Da-5000Da, and the particle size of the liposomes is limited to 20nm-200nm. The application can load twelve categories of functional active components and traditional Chinese medicine extracts, and is suitable for health foods, nutritional supplements, traditional Chinese medicine preparations and ordinary functional foods. The application optimizes carrier parameters and combination modes through tests, is not a simple superposition of prior art, has strong global adaptability, a simple process and high industrialization, can effectively improve the stability of active ingredients and the targeted delivery effect, has high technical barriers, and has high application and commercial values.

The invention relates to the field of pharmacy, in particular to a beta lactam antibiotic catechol derivative and preparation and application thereof. The beta lactam antibiotic catechol derivative is a compound shown in the formula I, and # imgabs0. The beta lactam antibiotic catechol derivative disclosed by the invention is prepared by taking dihydroxybenzoic acid as a catechol type siderophore, and coupling the catechol type siderophore with beta lactam antibiotics; the coupled antibiotics can be transported to the acting part in a targeted manner by utilizing a unique siderophore transport system of bacteria, and the drug effect can be gradually improved aiming at the drug sensitive flora of a parent nucleus. According to the invention, the drug with drug resistance can play the drug effect again, and drug resistance caused by insufficient antibiotic transport due to factors such as bacterial surface variation is avoided.