65results about How to "Improve intake efficiency" patented technology

The invention belongs to the field of biotechnology, and relates to a slightly acidic environment targeted polypeptide modified tumor targeted nano drug delivery system, and a preparation method thereof. The slightly acidic environment targeted polypeptide modified tumor targeted nano drug delivery system is prepared via self-assembly of a slightly acidic environment targeted polypeptide modified dendrimer encapsulated gene, wherein the surface of the dendrimer is rich of amino groups. According to the preparation method, a polypeptide of transmembrane helix protein C derived from bacteria visual purple is used for modifying a high molecular carrier, enrichment and adhesion onto cells is realized via a pH sensitive cell membrane insertion method, and entering into cells is realized via electrostatic adsorption guided endocytosis, so that untaking of tumor cells on drugs is improved, and toxic and side effects are reduced. According to the tumor targeted nano drug delivery system, cell membrane is taken as the target point, and the polypeptide is taken as the target head group in tumor slightly acidic environment, targeting and curing efficiency are high, the preparation method is simple and convenient, and tumor cell drugs, which are derived from human or animal, and is used for targeted therapy, can be prepared.

An evaporative cooler for cooling a gas stream, in particular an air stream, including a number of cooling elements located in a flow channel, is provided. A liquid, preferably water, is supplied by a feed device and will be vaporized or evaporated. In one aspect, the surface of at least one of the cooling elements has hydrophilic properties, at least in one sub-region designed to form a liquid film.

The invention belongs to the technical field of nano materials, and in particular relates to carboxymethyl chitosan nanoparticles modified with glycyrrhizic acid, as well as a preparation method and application thereof. The nanoparticles disclosed by the invention are in a core-shell type; a hydrophobic polymer serves as a kernel packaged medicament; and carboxymethyl chitosan serves as a hydrophilic shell and is covalently linked with hepatic targeting ligand glycyrrhizic acid. The preparation method comprises the following steps of: firstly grating and copolymerizing carboxymethyl chitosan and hydrophobic monomers and self-assembling to form nanoparticles in the action of an initiator; secondly, oxidizing glycyrrhizic acid to obtain a glycyrrhizic acid aldehyde solution, mixing the glycyrrhizic acid aldehyde solution with the nanoparticles at a certain proportion and introducing targeting groups, and carrying out ultrasonic loading on the medicament. Compared with the prior art, the preparation method is simple and easy to control and has good repeatability and better stability and safety; and the targeting property and the tumor-inhabiting effect of the anti-tumor medicament can be strengthened remarkably. The nanoparticles serve as a targeting delivery vector for hydrophobic medicaments or molecular probes and can be used for treatment and diagnosis of liver diseases.

An air intake device for a watercraft adapted to suppress noise generating from an intake system of an engine and can obtain good intake efficiency. The air intake device includes intake ports opening to a side surface of the engine, and an air cleaner located on the rear side of the engine. An outlet of the air cleaner is connected through an intake passage to the intake ports of the engine. An air duct extends from an inlet of the air cleaner along the side surface of the engine to the position corresponding to the front end of the engine. The air duct has a front opening that faces downward.

The invention provides an alpha helix cationic polypeptide as well as a preparation method and application of the alpha helix cationic polypeptide. The alpha helix cationic polypeptide takes a photosensitizer porphyrin as a core and alpha helix polypeptides as four arms, and a nucleotide drug prepared from the polymer can be applied to a nucleotide drug delivery system. The polymer provided by theinvention has good biocompatibility, low cytotoxicity and light activation ability. The polymer provided by the invention is self-assembled in a water solution to form nanoparticles which has good stability, biocompatibility and low cytotoxicity; the preparation method is simple and high in repeatability, and the polymer serving as a carrier is not only capable of protecting nucleic acid such asDNA from being degraded, but also capable of being combined with the scale effect of the nanoparticles so as to be used for treating diseases; and in addition, the transfection promoting effect for different cancer cells can be achieved by virtue of the light activation ability.

The invention provides antineoplastic hydrogel polypeptide and a preparation method. The polypeptide comprises a self-assembling structural domain at the C end and a killing structural domain at the Nend, and the self-assembling structural domain is connected with the killing structural domain through a flexible structural domain. According to an antineoplastic melittin hydrogel slow-releasing agentia, the antineoplastic hydrogel polypeptide carries antineoplastic active polypeptide to obtain the agentia. The preparation method comprises the following steps that 1), an antineoplastic hydrogelpolypeptide aqueous solution and an antineoplastic active polypeptide aqueous solution are prepared; 2), the antineoplastic hydrogel polypeptide aqueous solution and the antineoplastic active polypeptide aqueous solution which are prepared in the step 1) are mixed to obtain a mixed solution; 3), an NaCl solution is dropwise added into the mixed solution prepared in the step S2 until the concentration of the NaCl solution is 0.9%, and after standing, the antineoplastic melittin hydrogel slow-releasing agentia is prepared. The technical purposes of promotion of the uptaking efficiency of activepolypeptide cells, cytoplasm location, preparation of the antineoplastic hydrogel polypeptide and the preparation method can be achieved.

The invention discloses a structural molecule for affinity and target cell uptake capability of enhanced integrin receptor. The structural molecule has a molecular structure shown in a formula (I); the structural molecule is obtained by carrying out reaction between two cRGD oligopeptides and 6-aminocaproic acid, glutamate oligopeptide or/and arginine oligopeptide, 8-amino-3,6-dioxaoctanoic acid or 3-maleimidopropionic acid and the like to perform dehydration condensation on amino and carboxyl. According to the structural molecule disclosed by the invention, tumor cells expressing an alpha v beta 3 receptor and neovascular endothelial cells can be specially combined, the carried molecules such as nucleotide enter cytoplasm through receptor-mediated endocytic uptake, so that the uptake efficiency of expression cells of the alpha v beta 3 receptor is improved and the release of the carried molecules from a target cytoplasm endosome is promoted, and therefore, the structural molecule can be applied to preparation of targeted therapy drugs or disease diagnosis tracer agents.

Belonging to the tumor therapy protein related technical field, the invention specifically relates to a dendritic cell (DC) targeted affinity peptide TY peptide and application thereof. The TY peptideis 12 peptide, and the specific amino acid sequence is TITHFQFGPTVY. Based on the TY peptide, the invention further designs a novel nano-transportation system capable of targeting DC on the basis ofMSN, and loads the model antigen OVA and immunoadjuvant CpG to prepare the nano-carrier MSN(mesoporous silica nanoparticles)-TY/OVA/CpG successfully. Experiments indicate that: the MSN-TY/OVA/CpG canbe effectively ingested by immature DC, thus improving the efficiency of antigen ingestion by DC, promoting the maturation of DC and effectively activating DC, also effectively promoting antigen processing and presentation, stimulating secretion of cytokines, and further triggering antigen-specific CTL immunoreaction and anti-tumor immunoreaction.

The invention discloses a preparation method of a mesoporous apatite nano-drug carrier with reduction responsiveness and cellular targeting property for a hepatoma cell, belonging to the field of biomedical materials. The invention aims at solving the problems that the current drug carrier is high in toxic and side effects and low in degradability. The preparation method comprises the steps of firstly, preparing a template agent; secondly, preparing a mesoporous hydroxylapatite nanoparticle; thirdly, aminating mesoporous hydroxylapatite; fourthly, carboxylating mesoporous apatite; fifthly, preparing disulfide bond functionalized mesoporous apatite; and sixthly, preparing the mesoporous apatite nano-drug carrier with reduction responsiveness and cellular targeting property. The mesoporous apatite nano-drug carrier prepared by using the preparation method can be used for recognizing hepatoma cells in a targeting way, so that the uptake of the normal tissues to the drug is reduced, and the in-vivo toxic and side effects of the drug are reduced. The drug is regularly and quantitatively introduced to pathological tissues, so that the utilization ratio of the drug is increased.

The invention provides a preparation method of ultra-small-size iron oxide nanoparticles. The method comprises the following steps: dissolving ferric acetylacetonate in diethylene glycol with the concentration of 0.02-0.16 mol/L in the diethylene glycol, carrying out thermal decomposition on ferric acetylacetonate in an inert gas environment at 200-220 DEG C until a reaction system is converted into a black solution from reddish brown, and carrying out steps of heating reflux, cooling, separating and purifying. The invention further provides an application of the ultra-small-size iron oxide nanoparticles prepared by the preparation method in preparation of an MRI cell tracer. The preparation method is simple, the preparation method is simple, various parameters are easy to control, the prepared iron oxide nanoparticles are accurately controlled within the range of 2-5 nm, the prepared iron oxide nanoparticles are very sensitive to the influence of water quantum MRI signals of marked cells, the positive contrast of the ultra-small-size iron oxide nanoparticles serving as a contrast agent is enhanced, and positive enhancement is displayed for magnetically marked cells in an MRI image.

The invention relates to the technical field of immunotherapy or vaccine prevention and treatment, in particular to a heterocyclic compound containing two or more sulfur atoms and an application of the heterocyclic compound to preparation of a nano vaccine. The invention provides an application of the heterocyclic compound which contains at least two sulfur atoms and can be covalently or non-covalently connected with polypeptide to preparation of the nano vaccine. Nanoparticles prepared by self-assembly of the compound and an antigen can enter dendritic cytoplasm in a transmembrane mode, and the uptake efficiency of the antigen and an immunologic adjuvant is improved. In the process of entering the cells, biodegradation of an enzyme body in lysosome on antigens or nucleic acid adjuvants can be effectively avoided or reduced, so that the nano vaccine disclosed by the invention can efficiently activate the dendritic cells and improve the cross presentation effect on the antigens, further CD8 + T cells can be effectively activated, and proliferation of the T cells can be promoted. Therefore, the nano vaccine provided by the invention can prevent tumor cell proliferation and virus infection by utilizing efficient immune activation and immune regulation effects.

An intake pipe of an internal combustion engine includes a main flow passage allowing air introduced into the engine to flow therethrough, an upper branch flow passage branching off from the main flow passage and communicating with #2 cylinder, #4 cylinder, and #6 cylinder, a lower branch flow passage branching off from the main flow passage sand communicating with #1 cylinder, #3 cylinder, and #5 cylinder, and a partition wall allowing the upper branch flow passage and the lower branch flow passage to be apart. The partition wall has an end portion that faces the main flow passage. The end portion is formed of a spherical surface. With such a configuration, the intake pipe of the internal combustion engine, which intake pipe provides sufficient improvement in intake efficiency, is provided.

The invention relates to a preparation method of RI7217 and muscone co-modification DTX long-circulation lipidosome. The preparation method comprises the following steps of: S1: firstly, carrying out amination modification on a muscone ring, and enabling the amination muscone and distearoyl phosphatidyl acetamide-N-hydroxy succinimide-polyethylene glycol to react to synthesize and prepare distearoyl phosphatidyl ethanolamine-polyethylene glycol-muscone; and S2: taking distearoyl phosphatidyl ethanolamine-polyethylene glycol-maleimide maleophthalimide, distearoyl phosphatidyl ethanolamine-polyethylene glycol, distearoyl phosphatidyl ethanolamine-polyethylene glycol-muscone copolymers, egg yolk lecithin and cholesterol as materials, preparing the lipidosome by a film dispersion method, and then, through covalent linkage of a maleimide group on the surface of the lipidosome and sulfhydrylation RI7217, preparing RI7217-LP and RI7217-LP-M.

The invention discloses a biomimetic multifunctional lipoprotein nanoparticle modified by biological peptide and a preparation method and application thereof, the multifunctional biomimetic lipoprotein nanoparticle is composed of a biomimetic lipoprotein nano-carrier shell modified by biological peptide and a nano-form drug core, and the carrier shell is composed of apolipoprotein bionic peptide, biological peptide and natural phospholipid substitute. The preparation method comprises a film dispersion method and an emulsification evaporation method. The preparation method disclosed by the invention is simple and mild in condition and low in cost, and the prepared biological peptide modified biomimetic multifunctional lipoprotein nanoparticle has the advantages of high drug loading capacity, high biomimetic property, strong penetrability, biological safety, lesion site targeting property, drug loading mode diversity and the like. A nano-drug delivery platform can realize 'diagnosis and treatment integration' and 'multi-mode combined treatment', and can realize timely diagnosis and efficient treatment of complex progressive diseases such as tumors, neurodegenerative diseases and the like.

The invention discloses a preparation method and application of a nanocomposite material for inhibiting postoperative tumor recurrence. The method has the advantages of simple preparation, uniform distribution of nano-drugs, little influence on loaded drug activity and the like. At the same time, the prepared nanocomposite material has good mechanical properties and biocompatibility, and can be implanted in a body for long-term drug sustained release. Polycaprolactone is used as a bulk material, mesoporous silica nanoparticles are used as a drug carrier, doxorubicin is used as a chemotherapeutic drug, the biodegradable nanocomposite material doped with inorganic nano-drugs is prepared by a solvent-free low-temperature ball milling method, doped nanoparticles are uniformly distributed in the bulk polymer material, and the nanocomposite material has mechanical properties of the bulk polymer material. A new method is provided for preparing the nanocomposite material and realizing implantable localized delivery of anticancer drugs, and the nanocomposite material is hopeful to be applied to inhibition of postoperative tumor recurrence.

The invention provides an effective and safe preparation method of oral recombinant human lactoferrin sericin nanoparticles and an application. Uptake efficiency of rhLF cells can be improved, LPS induced macrophage inflammation can be inhibited, and DSS-induced acute colitis can be treated. The sericin nanoparticles containing rhLF are prepared from rhLF transgenic silkworm cocoons as a raw material, by sericin extraction, dialysis and ethanol induction, and are directly used for oral administration treatment of colitis. The sericin nanoparticles are suitable for large-scale production, simple to operate, and high in application value.

The invention discloses glutathione response type double-loading medicine polymer micelle and a preparation method and application thereof and belongs to the field of biological medical engineering materials. The preparation method disclosed by the invention is mild and simple and avoids special treatment. The prepared double-loading medicine polymer micelle disclosed by the invention has the advantages of good water solubility and good biocompatibility; furthermore, metabolite of the double-loading medicine polymer micelle is easy to degrade and has smaller toxicity; ingredients of the double-loading medicine polymer micelle are simple, the raw materials are easy to obtain, and the double-loading medicine polymer micelle is hopeful to be widely applied to the field of biological and medical engineering materials. The micelle can be used for wrapping hydrophobic drug; meanwhile, a positive-electricity high molecular material can carry gene medicine through electrostatic interaction toform a double-loading medicine carrier to give play to a synergistic effect. As polypeptide has a modifying effect, the polypeptide has the ability of entering tumor cells; furthermore, gene medicineand hydrophobic medicine can inhibit growth of tumor cells through different mechanisms, so that a treating effect of the polymer micelle to the tumor cells is improved; thus, the double-loading medicine polymer micelle has a wide application prospect in compound chemotherapy for malignant tumors.