Application of prussian blue nanoparticles in preparation of drugs for preventing, delaying or treating nervous system degenerative diseases

Abstract

The invention relates to an application of prussian blue nanoparticles in preparation of drugs for preventing, delaying or treating nervous system degenerative diseases. Researches find that the prussian blue nanoparticles have remarkable effects in the aspects of preventing, delaying or treating nervous system degenerative diseases. Cell level test results show that the prussian blue nanoparticles can reduce the ROS level in nerve cells stimulated by hydrogen peroxide and increase the proportion of living cells in the nerve cells stimulated by hydrogen peroxide; and animal level test resultsshow that the prussian blue nanoparticles can significantly reduce the expression level of oxidative stress markers in hippocampus of nervous system degenerative disease model mice, significantly improve the learning and memory ability of nervous system degenerative disease model mice, and improve dyskinesia. The preparation process of the prussian blue nanoparticles is simple, the macro production is easy, the reaction condition is mild, and the surface modification is easy.

Description

Technical field

[0001] The invention belongs to the technical field of biomedicine, and relates to a new application of Prussian blue nanoparticles, in particular to the application of Prussian blue nanoparticles in preparing drugs for preventing, delaying or treating degenerative diseases of the nervous system. Background technique

[0002] Nervous system degenerative diseases are irreversible neurological diseases caused by the loss of neurons in the brain and spinal cord. They mainly include Alzheimer's disease, Parkinson's disease, amyotrophic lateral sclerosis, Huntington's disease, etc. With the aging of society, the incidence of degenerative diseases of the nervous system is on the rise, causing huge social medical expenditure and family pressure. Most degenerative diseases of the nervous system still lack effective treatment methods. Therefore, finding effective methods to prevent, delay and treat such diseases is a problem that needs to be solved urgently.

[0003] Oxidat...

Examples

Embodiment 1

[0048] In this example, a Prussian blue nanoparticle without functional modification was prepared. The preparation method includes the following steps:

[0049] (1) Dissolve 0.01 mM potassium ferrocyanide and 0.001 mM m PEG-COOH (MW5000) in 5 mL deionized water, mix well to obtain a clear solution A; dissolve 0.01 mM ferric chloride in 5 mL deionized water, Fully dissolve to obtain clear solution B; add solution B dropwise to solution A, and react at 60°C for 1 hour;

[0050] (2) After the reaction system is lowered to 25°C, react for 1 hour, centrifuge and wash to obtain Prussian blue nanoparticles without functional modification.

[0051] The prepared Prussian blue nanoparticles were characterized by particle size and potential. The result was that the dynamic light scattering particle size was 80nm and the surface potential was -32mV.

Embodiment 2

[0053] In this example, a single-targeted modified Prussian blue nanoparticle was prepared. The preparation method includes the following steps:

[0054] (1) Dissolve 0.01 mM potassium ferrocyanide and 0.001 mM m PEG-COOH (MW5000) in 5 mL deionized water, mix well to obtain a clear solution A; dissolve 0.01 mM ferric chloride in 5 mL deionized water, Fully dissolve to obtain clear solution B; add solution B dropwise to solution A, and react at 60°C for 1 hour;

[0055] (2) After the reaction system is lowered to 25°C, react for 1 hour, centrifuge and wash, to obtain Prussian blue nanoparticles without functional modification;

[0056] (3) Dissolve the obtained Prussian blue nanoparticles, 0.01 mM 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride in 10 mL of deionized water, and react at 25°C for 15 minutes to obtain a solution D;

[0057] (4) Add 0.01mM N-hydroxysuccinimide and 0.4μM Congo red to solution D, react at 25°C for 24h, centrifuge and wash, and the resulting pre...

Embodiment 3

[0060] In this example, a dual-targeted modified Prussian blue nanoparticle was prepared. The preparation method includes the following steps:

[0061] (1) Dissolve 0.01 mM potassium ferrocyanide and 0.001 mM m PEG-COOH (MW5000) in 5 mL deionized water, mix well to obtain a clear solution A; dissolve 0.01 mM ferric chloride in 5 mL deionized water, Fully dissolve to obtain clear solution B; add solution B dropwise to solution A, and react at 60°C for 1 hour;

[0062] (2) After the reaction system is lowered to 25°C, react for 1 hour, centrifuge and wash, to obtain Prussian blue nanoparticles without functional modification;

[0063] (3) Dissolve the obtained Prussian blue nanoparticles, 0.01 mM 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride in 10 mL of deionized water, and react at 25°C for 10 minutes to obtain a solution D;

[0064] (4) Add 0.01mM N-hydroxysuccinimide, 0.005mg transferrin, and 0.5μM Congo red to solution D, react at 25°C for 24h, centrifuge and wash, a...

Technical field

[0001] The invention belongs to the technical field of biomedicine, and relates to a new application of Prussian blue nanoparticles, in particular to the application of Prussian blue nanoparticles in preparing drugs for preventing, delaying or treating degenerative diseases of the nervous system. Background technique

[0002] Nervous system degenerative diseases are irreversible neurological diseases caused by the loss of neurons in the brain and spinal cord. They mainly include Alzheimer's disease, Parkinson's disease, amyotrophic lateral sclerosis, Huntington's disease, etc. With the aging of society, the incidence of degenerative diseases of the nervous system is on the rise, causing huge social medical expenditure and family pressure. Most degenerative diseases of the nervous system still lack effective treatment methods. Therefore, finding effective methods to prevent, delay and treat such diseases is a problem that needs to be solved urgently.

[0003] Oxidat...

Examples

Embodiment 1

[0048] In this example, a Prussian blue nanoparticle without functional modification was prepared. The preparation method includes the following steps:

[0049] (1) Dissolve 0.01 mM potassium ferrocyanide and 0.001 mM m PEG-COOH (MW5000) in 5 mL deionized water, mix well to obtain a clear solution A; dissolve 0.01 mM ferric chloride in 5 mL deionized water, Fully dissolve to obtain clear solution B; add solution B dropwise to solution A, and react at 60°C for 1 hour;

[0050] (2) After the reaction system is lowered to 25°C, react for 1 hour, centrifuge and wash to obtain Prussian blue nanoparticles without functional modification.

[0051] The prepared Prussian blue nanoparticles were characterized by particle size and potential. The result was that the dynamic light scattering particle size was 80nm and the surface potential was -32mV.

Embodiment 2

[0053] In this example, a single-targeted modified Prussian blue nanoparticle was prepared. The preparation method includes the following steps:

[0054] (1) Dissolve 0.01 mM potassium ferrocyanide and 0.001 mM m PEG-COOH (MW5000) in 5 mL deionized water, mix well to obtain a clear solution A; dissolve 0.01 mM ferric chloride in 5 mL deionized water, Fully dissolve to obtain clear solution B; add solution B dropwise to solution A, and react at 60°C for 1 hour;

[0055] (2) After the reaction system is lowered to 25°C, react for 1 hour, centrifuge and wash, to obtain Prussian blue nanoparticles without functional modification;

[0056] (3) Dissolve the obtained Prussian blue nanoparticles, 0.01 mM 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride in 10 mL of deionized water, and react at 25°C for 15 minutes to obtain a solution D;

[0057] (4) Add 0.01mM N-hydroxysuccinimide and 0.4μM Congo red to solution D, react at 25°C for 24h, centrifuge and wash, and the resulting pre...

Embodiment 3

[0060] In this example, a dual-targeted modified Prussian blue nanoparticle was prepared. The preparation method includes the following steps:

[0061] (1) Dissolve 0.01 mM potassium ferrocyanide and 0.001 mM m PEG-COOH (MW5000) in 5 mL deionized water, mix well to obtain a clear solution A; dissolve 0.01 mM ferric chloride in 5 mL deionized water, Fully dissolve to obtain clear solution B; add solution B dropwise to solution A, and react at 60°C for 1 hour;

[0062] (2) After the reaction system is lowered to 25°C, react for 1 hour, centrifuge and wash, to obtain Prussian blue nanoparticles without functional modification;

[0063] (3) Dissolve the obtained Prussian blue nanoparticles, 0.01 mM 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride in 10 mL of deionized water, and react at 25°C for 10 minutes to obtain a solution D;

[0064] (4) Add 0.01mM N-hydroxysuccinimide, 0.005mg transferrin, and 0.5μM Congo red to solution D, react at 25°C for 24h, centrifuge and wash, a...

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