Application of Pinctada martensii mucus in extraction of exosome, exosome as well as extraction method and application of exosome

A technology of Pinctada martensii and an extraction method, applied in the biological field, can solve problems such as lack, and achieve the effect of improving the utilization value
CN114480249APending Publication Date: 2022-05-13GUANGDONG OCEAN UNIVERSITY +1
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
GUANGDONG OCEAN UNIVERSITY
Publication Date
2022-05-13

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Abstract

The invention provides application of pinctada martensii mucus to extraction of exosomes, the exosomes and an extraction method and application of the exosomes. According to the method, the vesicular nanoparticles are separated from the pinctada martensii mucus by adopting an ultracentrifugation method and are identified as the exosome, so that reference is provided for extracting the bioactive exosome from marine organisms, and meanwhile, the utilization value of the pinctada martensii is also improved. The exosome extracted from the pinctada martensii mucus can enhance the cell viability, reduce the ROS level in cells, inhibit the activation of NF-kappa B and NLRP3 pathways and reduce the expression level of related inflammatory factors mRNA, and has a certain anti-inflammatory effect.
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Description

technical field

[0001] The present invention relates to the field of biotechnology, in particular, to the use of Pinctada martensii mucus for extracting exosomes, exosomes and its extraction method and application. Background technique

[0002] Exosomes and extracellular vesicles are spherical nanoparticles composed of lipids, proteins, nucleic acids and other biological factors. At present, there is no clear classification of extracellular vesicles and exosomes in the world. Usually, they are called For vesicular nanoparticles. As a vesicular nanoparticle secreted by cells, exosomes can transmit material information between cells, so exosomes have been used in the treatment of various diseases, such as anti-tumor, anti-inflammation and anti-oxidation. Since exosomes are cell products, the use of exosomes as a therapeutic approach is less cytotoxic and more biocompatible than other therapeutic approaches.

[0003] Exosomes are distributed in the body fluids and cells of ma...

Claims

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