Roxburgh rose pomace combined phenol with remarkable antioxidant activity as well as preparation method and application thereof

A technology of antioxidant activity and prickly pear pomace is applied in the directions of food ingredients as antioxidants, medical preparations containing active ingredients, applications, etc. High productivity, increased survival effect

Active Publication Date: 2022-02-25
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the extraction, substance identification, and antioxidant activity of roxburghii pomace combined with phenols in vivo and in vitro.

Method used

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  • Roxburgh rose pomace combined phenol with remarkable antioxidant activity as well as preparation method and application thereof
  • Roxburgh rose pomace combined phenol with remarkable antioxidant activity as well as preparation method and application thereof
  • Roxburgh rose pomace combined phenol with remarkable antioxidant activity as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] A preparation of roxburghii pomace binding phenol with significant antioxidant activity, comprising the following steps:

[0047] (1) Raw material pretreatment: wash the pomace of Rosa roxburghii and put it on a tray, dry it in a blast drying oven at 45°C for 48 hours, and pass it through a 40-mesh sieve after crushing;

[0048] (2) Degreasing: Mix the dry powder of Rosa roxburghii pomace with n-hexane at a mass volume ratio of 1:10 g / mL, shake at room temperature for 2 h, centrifuge at a centrifugal force of 6000 g for 5 min, collect the residue, and repeat the operation 6 times. The residue was dried in a blast drying oven at 45°C for 48 hours to obtain dry powder of degreased thorn pear pomace;

[0049] (3) Removal of free phenols: mix the dry powder of degreased roxburghii pomace with a mass-to-liquid ratio of 1:15 g / mL and ethanol with a volume concentration of 70%, extract at room temperature for 2 h, centrifuge at 6000 g for 5 min, and discard the upper The clea...

Embodiment 2

[0052] A preparation of roxburghii pomace binding phenol with significant antioxidant activity, comprising the following steps:

[0053] (1) Raw material pretreatment: wash the pomace of Rosa roxburghii and put it on a tray, dry it in a blast drying oven at 45°C for 48 hours, and pass through a 50-mesh sieve after crushing;

[0054] (2) Degreasing: Mix the dry powder of Rosa roxburghii pomace with n-hexane at a mass-to-liquid ratio of 1:20 g / mL, shake at room temperature for 1.5 h, centrifuge at a centrifugal force of 5000 g for 10 min, collect the residue, and repeat the operation 5 times , the residue was dried in a blast oven at 45°C for 48 hours to obtain a dry powder of degreased thorn pear pomace;

[0055] (3) Removal of free phenols: mix the dry powder of degreased roxburghii pomace with a mass-to-liquid ratio of 1:10 g / mL and ethanol with a volume concentration of 70%, extract at room temperature for 3 h, centrifuge at 5000 g for 10 min, and discard the upper The clea...

Embodiment 3

[0058] A preparation of roxburghii pomace binding phenol with significant antioxidant activity, comprising the following steps:

[0059] (1) Raw material pretreatment: wash the pomace of Rosa roxburghii and put it on a tray, dry it in a blast drying oven at 45°C for 48 hours, crush it and pass it through a 60-mesh sieve;

[0060] (2) Degreasing: Mix the dry powder of Rosa roxburghii pomace with n-hexane at a mass-to-liquid ratio of 1:30 g / mL, shake at room temperature for 1 h, centrifuge at a centrifugal force of 4000 g for 15 min, collect the residue, and repeat the operation 4 times. The residue was dried in a blast drying oven at 45°C for 48 hours to obtain dry powder of degreased thorn pear pomace;

[0061] (3) Removal of free phenols: mix the dry powder of degreased roxburghii pomace with a mass-to-liquid ratio of 1:20 g / mL and ethanol with a volume concentration of 70%, extract at room temperature for 1 h, centrifuge at 4000 g for 15 min, and discard the upper The clear...

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Abstract

The invention discloses roxburgh rose pomace combined phenol with remarkable antioxidant activity as well as a preparation method and application of the roxburgh rose pomace combined phenol. The method comprises the following steps: mixing roxburgh rose pomace dry powder with n-hexane, shaking, centrifuging, collecting residues, and drying to obtain degreased roxburgh rose pomace dry powder; mixing the degreased roxburgh rose pomace dry powder with ethanol, and extracting at normal temperature; mixing the obtained residues with a 2-4mol/L NaOH solution, introducing protective gas, sealing, extracting at room temperature for 1-2 hours, adjusting the pH value of the solution to 2-4 by using HCl to obtain a mixed solution, extracting by using ethyl acetate, centrifuging, collecting the ethyl acetate phase, concentrating under reduced pressure, and freeze-drying to obtain the roxburgh rose pomace combined phenol extract. The roxburgh rose pomace combined phenol extract prepared by the invention is high in purity and rich in phenolic substances, has relatively high DPPH and ABTS free radical scavenging ability and oxygen free radical absorption ability, and can also be used in healthy foods or medicines for adjuvant therapy of oxidative stress related diseases.

Description

technical field [0001] The invention belongs to the field of health food, and in particular relates to a roxburghii pomace-bound phenol with significant antioxidant activity, a preparation method and application thereof. Background technique [0002] Prickly pear (Rosa Roxburghii Tratt) is a fruit crop distributed in mountainous areas with an altitude of 500-2500 meters in southwest China, especially in Guizhou. Rosa roxburghii is rich in polysaccharides, ascorbic acid, polyphenols, superoxide dismutase and other nutrients, and has a long history of both medicine and food. In recent years, many studies have confirmed that Rosa roxburghii has various biological activities such as anti-oxidation, anti-atherosclerosis, hypoglycemia, anti-aging, anti-tumor, etc., which has attracted widespread attention. By 2020, the planting area of ​​thorn pears in Guizhou will expand to 2 million mu, the output of fresh fruit and related products will reach 100,000 tons and 26,200 tons respe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/105A23L2/52A23L3/3472A61K36/738A61P39/06A61K131/00
CPCA23L33/105A23L2/52A23L3/3472A61K36/738A61P39/06A23V2002/00A61K2236/13A61K2236/17A61K2236/15A61K2236/35A61K2236/51A61K2236/333A61K2236/39A61K2236/53A23V2200/30A23V2200/02A23V2250/2132
Inventor 李超黄丹思扶雄黄强
Owner SOUTH CHINA UNIV OF TECH
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