32results about How to "Improve acid stress resistance" patented technology

The invention discloses a method for using the transcription factor Crz1p to regulate acid stress resistance of Toruula glabrata, belonging to the field of bioengineering. In the present invention, by deleting or overexpressing the Cgcrz1 gene of Torulopsis glabrata, the acid stress resistance of the strain is correspondingly reduced or improved. The present invention also compares the cell membrane fatty acid, sterol composition, ratio and permeability of the deletion mutant strain Cgcrz1Δ under acid stress. Acid stress resistance.

The invention discloses a method for improving the acid stress resistance of recombinant Escherichia coli, which belongs to the technical field of microbial engineering. The present invention uses the gene of trehalose 6-phosphate hydrolase TreC as the target gene and Escherichia coli as the expression host to successfully construct a class of Escherichia coli engineering bacteria that can be widely used in the preparation of food, medicine, feed and chemicals; the Escherichia coli The itaconic acid stress resistance of Bacillus engineered bacteria has been significantly improved, up to 163.5 times higher than wild strains; the succinic acid stress resistance of Escherichia coli engineered bacteria has been significantly improved, up to 2.3 times higher than wild strains.

The invention discloses a glyceraldehyde-3-phosphate dehydrogenase and applications thereof, and belongs to the technical field of genetic engineering and microbial engineering. The glyceraldehydes-3-phosphate dehydrogenase with an amino acid sequence as shown in SEQ ID No.1 has the function of helping microorganisms produce triacylglycerol (TAG). By culturing the recombinant Mortierella alpina containing the glyceraldehydes-3-phosphate dehydrogenase in a shaking table for 7 days, the total fatty acid content and the polyunsaturated fatty acid content of the Mortierella alpina containing the glyceraldehydes-3-phosphate dehydrogenase can be increased by 12.56% and 12.79% respectively, compared with the Mortierella alpina containing no glyceraldehydes-3-phosphate dehydrogenase. The result provides abundant theoretical support for further improving the capability of oil-producing microorganisms such as Mortierella alpina to produce triacylglycerol (TAG) and further improving the capability of microorganisms to produce polyunsaturated fatty acid (PUFAs) through a genetic engineering means.

The invention discloses acid stress resistant recombinant lactic acid bacteria and application thereof, and belongs to the field of bioengineering technology. By overexpression of TraG gene derived from L.lactis NZ9000 in L.lactis NZ9000, the recombinant L.lactis NZ9000 (pNZ8148 / TraG) with acid stress resistance remarkably enhanced is obtained. By stress for 3 h at pH 4.0, survival rate of the recombinant strain is 61.3 times higher than survival rate of a contrast. The invention also provides a method for raising acid stress resistance. The method of the invention has good industrial application value.

The invention discloses an acid-stress-resisting component and belongs to the technical field of biological engineering. An rbsA gene obtained from lactococcus lactis (L.lactis NZ9000) is excessivelyexpressed in the lactococcus lactis (L.lactis NZ9000) to obtain recombinant lactococcus lactis (L.lactis NZ9000) (pNZ8148 / RbsA) with the remarkably improved acid-stress-resisting capability. The survival rate of a recombinant strain is 5.8 times as much as that of a control group after the recombinant strain is stressed for 3h under the condition that the pH (Potential of Hydrogen) is 4.0. The invention further provides a method for improving the acid stress resistance; the method has good industrial application value.

The invention discloses a lactic acid bacteria engineering bacterium with improved acid stress resistance and an application thereof, belonging to the technical fields of genetic engineering and microbial engineering. The present invention takes the gene encoding metal ABC transporter permease ZitP (metal ABC transporter permease) as the target gene, and uses lactic acid bacteria as the expression host to successfully construct a kind of protein that can be widely used in the preparation of food, medicine, feed and chemicals. Lactic acid bacteria engineering bacteria: The acid stress resistance of this lactic acid bacteria engineering bacteria has been significantly improved, up to 14.5 times higher than that of wild strains.

The invention discloses lactic acid bacteria engineering bacteria with improved acid stress resistance, and belongs to the genetic engineering and microbial engineering technical fields. The lactic acid bacteria engineering bacteria which can be widely applied in preparation of food, medicines, feeds and chemicals are successfully constructed by taking a gene encoding phosphoribosylaminimidazole-succinimide synthetase PurC as a target gene and lactic acid bacteria as an expression host. The acid stress resistance of the lactic acid bacteria engineering bacteria is significantly improved, and the maximum acid stress resistance is improved by 83.2 times than that of wild strains.

The invention discloses acid stress resistant recombinant lactic acid bacteria and a construction method thereof, and belongs to the field of bioengineering technology. By overexpression of LtrC genederived from L.lactis NZ9000 in L.lactis NZ9000, the recombinant L.lactis NZ9000 (pNZ8148 / LtrC) with acid stress resistance remarkably enhanced is obtained. By stress for 3 h at pH 4.0, survival rateof the recombinant strain is 3.6 times higher than survival rate of a contrast. The invention also provides a method for raising acid stress resistance. The method of the invention has good industrialapplication value.

The invention discloses an acid stress resistant component and application thereof, and belongs to the field of bioengineering technology. By overexpression of CmhR gene derived from L. lactis NZ9000in L. lactis NZ9000, recombinant L. lactis NZ9000 (pNZ8148 / CmhR) with acid stress resistance remarkably enhanced is obtained. By stress for 3 h at pH 4.0, survival rate of the recombinant strain is 12.3 times higher than survival rate of a contrast. The invention also provides a method for raising acid stress resistance. The method of the invention has good industrial application value.

The invention discloses a method for improving the acid stress resistance of Serratia marcescens, which belongs to the technical fields of genetic engineering and microbial engineering. The present invention significantly improves the acid stress resistance of Serratia marcescens by overexpressing the DNA-binding protein XrpA in Serratia marcescens; The survival rate after culturing for 12 hours under the environment of 4.0 was 1.4 times that of the wild type Serratia marcescens.

The invention discloses a method for improving the acid stress resistance of T. glabrata, belonging to the field of biological engineering. The present invention constructs a gene deletion mutant strain Cgade12Δ by transforming T. glabrata, measures the intracellular ATP level of the constructed strain, and analyzes the organic acid tolerance of the strain through a plate growth experiment and cell activity. After the deletion of the gene CgADE12 was found, under 0.2% acetic acid stress, the intracellular ATP level of the strain decreased, the growth ability under acetic acid stress increased, and the acetic acid tolerance was enhanced.

The invention discloses a Lactobacillus engineered bacterium with improved acid stress resistance and application thereof, and belongs to the technical field of genetic engineering and microbial engineering. With a gene of an encoded energy-coupling factor transporter ATP enzyme cfA2 serving as a target gene and Lactobacillus as an expression host, the Lactobacillus engineered bacterium widely applicable to the preparation of foods, drugs, feeds and chemicals is successfully constructed; acid stress resistance of the Lactobacillus engineered bacterium is evidently improved, which is 598.7 times as high as that of a wild strain.

The invention discloses an anti-acid-stress component and application thereof, belonging to the field of bioengineering technology. According to the invention, two recombinant Lactococcus lactis strains with substantially improved resistance to acid stress, i.e., L. lactis NZ9000 (pNZ8148 / RecT-A76) and L. lactis NZ9000 (pNZ8148 / RecT-MG1655), are obtained by overexpression of recT genes, originated from Lactococcus lactis L. lactis A76 and Escherichia coli E. coli MG1655, in Lactococcus lactis NZ9000, respectively. After the two recombinant Lactococcus lactis strains suffer from stress in an environment with a pH value of 4.0 for 3h, the survival rates of the two strains are 10.5 times and 1.4 times the survival rates of control strains, respectively; and after the two strains suffer from stress caused by 20% (v / v) ethanol for 6 h, the survival rates of the two strains are 3.7 times and 2.1 times the survival rates of control strains, respectively. The invention also provides a method for improving resistance to acid stress. The method has good industrial application value.

The invention discloses an Escherichia coli engineering bacterium with improved acid stress ability and its application, belonging to the technical field of microbial engineering. The present invention uses the gene of the nickel ion ABC transporter periplasmic binding protein NikA as the target gene and Escherichia coli as the expression host to successfully construct a class of Escherichia coli engineering bacteria that can be widely used in the preparation of food, medicine, feed and chemicals; The acid stress ability of the strain has been significantly improved, and its resistance to itaconic acid is 6.3 times that of the control strain; the resistance to D-lactic acid is 3.3 times that of the control strain; the resistance to succinic acid is 3.3 times that of the control strain 1.6 times.