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A lactic acid bacteria fermentation medium for feed and a preparation method thereof

A lactic acid bacteria fermentation and culture medium technology, applied in the field of microorganisms, to achieve the effects of low production costs, extended growth needs, and convenient operation

Inactive Publication Date: 2019-01-15
黄拥亮
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The high-density culture of lactic acid bacteria has been widely used in the dairy industry, but the research and application in the food industry has just started, and there are still many technical problems to be solved, especially the fermentation medium of lactic acid bacteria for feeding needs to be optimized

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0035] Preparation of whey protein hydrolysate: acid hydrolyze the whey protein added with inulin;

[0036] The obtained whey protein hydrolysate, yeast powder, glucose, buffer salt, Mg 2+ Water-soluble compound, Fe 2+ Water-soluble compound, MnSO 4 Mix evenly to obtain lactic acid bacteria fermentation medium for feed.

[0037] In one embodiment of the present invention, the specific steps for preparing the whey protein hydrolysate are: adding a concentration of 3.5-4.5 mol / L to the whey protein added with inulin according to a material-to-liquid ratio of 1:4.2-5.8 The hydrochloric acid solution is condensed and refluxed at 95-105°C for 10-15h for acid hydrolysis. The whey protein hydrolysate obtained by the preparation method has high nutritional value, and contains the umami-flavored amino acids glutamic acid and aspartic acid and the sweet-flavored amino acids serine, glycine, threonine and alanine. , Proline, can improve the utilization rate of feed lactic acid bacteria to th...

Embodiment 1

[0041] A feed lactic acid bacteria fermentation medium containing whey protein hydrolysate; the above-mentioned whey protein hydrolysate is obtained by acid hydrolyzing whey protein with inulin; wherein the degree of polymerization of inulin is 15, containing 2.7% γ-inulin; the addition amount of inulin is 5.8% of the weight of whey protein.

[0042] The fermentation medium contains 20g / L whey protein hydrolysate, 2g / L yeast powder, 14g / L glucose, 5g / L buffer salt, and Mg 2+ Water-soluble compound 0.3g / L, Fe 2+ Water-soluble compound 0.1g / L, MnSO 4 0.04g / L; Among them, Mg 2+ Water-soluble compound is MgSO 4 ; Fe 2+ The water-soluble compound is FeSO 4 ; Buffer salt is sodium citrate.

[0043] A method for preparing a fermentation medium for lactic acid bacteria for feed, comprising:

[0044] Preparation of whey protein hydrolysate: add a 3.5 mol / L hydrochloric acid solution to the whey protein added with inulin according to the material-to-liquid ratio of 1:4.2, and reflux for 10 ho...

Embodiment 2

[0047] A feed lactic acid bacteria fermentation medium containing whey protein hydrolysate; the above-mentioned whey protein hydrolysate is obtained by acid hydrolyzing whey protein with inulin; wherein the degree of polymerization of inulin is 17, containing 3.2% γ-inulin; the addition amount of inulin is 6.4% of the weight of whey protein.

[0048] The fermentation medium contains 26g / L whey protein hydrolysate, 4g / L yeast powder, 20g / L glucose, 7.5g / L buffer salt, and Mg 2+ Water-soluble compound 0.4g / L, Fe 2+ Water-soluble compound 0.2g / L, MnSO 4 0.05g / L; Among them, Mg 2+ Water-soluble compound is MgCl 2 ; Fe 2+ The water-soluble compound is FeCl 2 ; Buffer salts are potassium dihydrogen phosphate, sodium citrate and sodium acetate in a weight ratio of 1:0.8:0.4.

[0049] A method for preparing a fermentation medium for lactic acid bacteria for feed, comprising:

[0050] Preparation of whey protein hydrolysate: add a 4.0mol / L hydrochloric acid solution to the whey protein added...

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Abstract

The invention provides a lactic acid bacteria fermentation medium for feed and a preparation method thereof, belonging to the technical field of microorganisms, wherein the fermentation medium comprises whey protein hydrolysate obtained by acid hydrolysis of whey protein with inulin. The preparation method of fermentation medium comprises: preparing whey protein hydrolysate: carrying out acid hydrolysis on whey protein added with inulin; and mixing the resulting whey protein hydrolysate, yeast powder, glucose, buffer salt, Mg<2+> water-soluble compound, Fe<2+> water-soluble compound, and MnSO4. As that fermentation medium of the invention can prevent the damage of oxygen to the feed lactic acid bacteria, more ATP is provided for the feeding lactic acid bacteria, the growth requirement ofthe fermentation medium for the feed lactic acid bacteria is prolonged, the acid stress resistance and the propagation quantity of the feeding lactic acid bacteria are improved, and high-density culture is realized. The whey protein hydrolysate prepared by the preparation method of the invention comprises D Type amino acids and flavoring amino acids.

Description

Technical field [0001] The invention belongs to the technical field of microorganisms, and specifically relates to a feed lactic acid bacteria fermentation medium and a preparation method thereof. Background technique [0002] In the new century, my country's livestock and poultry breeding industry has developed rapidly. The process of large-scale farming is getting faster and faster, and scientific farming has also been widely used. But the drawbacks that have always existed in the breeding industry have not been well resolved. For example, drug residues and bacterial resistance caused by long-term abuse of antibiotics are potentially harmful to human health. Furthermore, the feces and sewage produced in livestock and poultry breeding, if not properly treated or used, will seriously pollute the environment and affect human survival. The proposal and in-depth study of microecology theory is the main method to solve the problems in the aquaculture industry, and it has gradually...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20
CPCC12N1/20
Inventor 黄拥亮
Owner 黄拥亮
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