76results about How to "Increase reproductive number" patented technology

The invention relates to the technical field of grape seedling cultivation, in particular to a grape seedling cultivating method. The grape seedling cultivating method includes the following steps that firstly, in early April, a grape seedling is cut into a grape shoot for cutting, wherein the length of the grape shoot for cutting ranges from 0.5 m to 0.8 m, and 4 to 6 bud eyes are reserved in the grape shoot for cutting, and then the grape shoot for cutting is disinfected through an ultraviolet sterilization lamp, wherein ultraviolet disinfection time ranges from 15 min to 30 min; secondly, a seedling bag filled with nutrient soil is placed on a seedbed, and the nutrient soil is completely irrigated through drop irrigation, wherein irrigation time ranges from 1 hour to 4 hours, the grape shoot for cutting is inserted into the nutrient soil after irrigation, and the bud eyes of the grape shoot for cutting are exposed out of the nutrient soil. According to the grape seedling cultivating method, seedling cultivation is conducted again in the seedling cultivation bag, nutrition supplied to the grape shoot for cutting is more sufficient, the grape shoot for cutting grows better, the excellent characters of a grape stock plant can be kept, the seedling reproduction number is increased by 50% in average compared with that of a root burying seedling cultivating method, and the yield obtained after fruiting is increased by 40% in average.

The invention discloses a method for building a leaf in vitro regeneration system of begonia rex. The method comprises the following steps of: (1) selecting an explant and sterilizing the explant; (2) carrying out adventitious bud induced cultivation after cutting the processed leaves; (3) carrying out high-voltage electrostatic field treatment; (4) carrying out subculture proliferation cultivation on the adventitious bud induced by the leaves; (5) carrying out rooting culturing on a test-tube plantlet after the subculture proliferation cultivation; and (6) cutting and rooting. The begonia rex is subjected to tissue culturing by adopting the propagation method; the reproduction rate of a culturing period (51d) is 243.1 times; the propagation speed is fast; and the problem of seeding propagation of the begonia rex is effectively solved.

A medium-and-low temperature ground heat and biomass gas combined power generation system is characterized in that a working medium evaporator is connected with a working medium superheater, a prime motor and a power generator of a gas boiler, the prime motor is communicated with a working medium condenser and a working medium storage tank, and the working medium storage tank is communicated with a working medium preheater of the gas boiler through a working medium booster pump; a production well is communicated with a ground heat water processing device and the working medium evaporator, and the working medium evaporator is communicated with a second inner heating pipe and a first inner heating pipe; the first inner heating pipe is communicated with an inverted well, an underground heat storage part and the production well; biomass is communicated with an acidification hydrolysis tank and communicated with a biogas residue fermentation tank and biogas fertilizer through a biogas residue circulation pump, and the biogas residue fermentation tank is communicated with a gas storage tank through a first gas booster pump; the acidification hydrolysis tank is communicated with an anaerobic digestor through an organic acid circulation pump; the anaerobic digestor is communicated with the gas storage tank and a combustor in the gas boiler through a second gas booster pump; and an anaerobic digester filling module is arranged in the anaerobic digestor. A power generation cost calculating method for the medium-and-low temperature ground heat and biomass gas combined power generation system is provided.

The invention discloses a begonia masoniana leaf in-vitro regeneration system establishment method, which is characterized by comprising: selection and disinfection of explants, adventitious bud induction, adventitious bud subculture to obtain a test tube plantlet, root induction, and transplantation. According to the present invention, with the application of the breeding method to carry out tissue culture on the begonia masoniana, the one culture period proliferation rate is more than 1200 times, the breeding is fast, and the difficult problem of the begonia masoniana seedling breeding is effectively solved.

The invention belongs to the field of agricultural technology, and in particular relates to a rapid breeding method for apple non-toxic dwarf self-root stock M9‑T337 stock. The method comprises the following concrete steps: construction of stock parent gardens; construction of breeding gardens; construction of demonstration gardens, and construction of modern apple cultivation demonstration gardens according to the standards of "four reforms, three transformations and three festivals". The invention adopts the layering technology of the same year of planting, shortens the time for seedlings to emerge from the nursery, and improves the breeding efficiency of seedlings. Adopt the method of flat stubble when the new seedling grows to 20cm, promote it to increase 2-5 new shoots, and when most of the new shoots grow to 30cm, 40cm, and 50cm on the seedling stem, press the decomposed sawdust 10cm/time in 3 times, and then Cultivate 5cm of soil to promote new roots of new stock seedlings. Compared with the foreign method of mixing soil with sawdust, it saves labor, time and effort. At the same time, this breeding method shortened the breeding time of non-toxic M9-T337 apple dwarf self-root stock by 50%, and increased the number of reproduction by more than 2 times.

The invention discloses a blueberry tissue culture method. The method includes the following steps that tender stems or stem tips of annual vegetative shoots are selected, leaves are cut off in a laboratory, and the stems or stem tips are sterilized and cut into stem segments which are 2 cm long; the sterilized stem segments are moved into wild-mouth bottles containing solid culture media, a sterile system is built, MS culture media are used, sealing is conducted, and the culture bottles are placed in a culture room to be cultured; by means of culture preservation, a sterile system of blueberries is basically built, and at this time, a subculture process starts; tissue culture seedlings obtained after subculture enter the rooting culture stage, and an in-bottle rooting mode is adopted; the rooted tissue culture seedlings are conveyed into a transitional greenhouse for transition by means of transition matrixes, during transplantation, the culture media on roots of the tissue culture seedlings are cleaned away, then the tissue culture seedlings are transplanted on the matrixes and covered with a small shed after transplantation, and transitional culture is conducted for 15-20 d. The rooting rate can reach 85% or above, and the survival rate of transplantation can reach 90% or above.

The invention discloses a method for cultivating sweet potato seedlings, and the planting time is from the middle ten days of November every year to the June the next year. The method comprises the following steps that (1) a ploughed field is ridged; (2) preprocessing is carried out on shorn sweet potato seedlings, the sweet potato seedlings on every two adjacent ridges are planted oppositely, and then the sweet potato seedlings are watered; (3) when the outdoor temperature is lower than or equal to15 DEG C, a tunnel is built between every two adjacent ridges with bamboo sticks, the tunnels are covered with films, and the films are removed when the outdoor temperature keeps at 25 DEG C or above. Through the method for cultivating sweet potato seedlings, the planting time of spring sweet potatoes is advanced by 20-30 d compared with a potato block seedling cultivation method, the breeding frequency of sweet potato seedlings is increased, yield of the sweet potato seedlings is increased, cultivation of new species is further facilitated, a large number of seedlings can be cultivated within a short time through the method, and the application and popularization speed is improved. In addition, the method can improve the survival and growth capacity of sweet potatoes at low temperature and further improve the utilization rate of land.

The invention discloses a soilless propagation method of monopterus albus Zuiew. The soilless propagation method comprises the following steps: setting an opened net cage which is not less than 15m<2> in area in a pond or a greenhouse, putting a pile of round myriophyllum aquaticum in the net cage every 1.5m, putting two parent monopterus albus Zuiew in each pile of the myriophyllum aquaticum, feeding mixed bait every 2 to 3 days, and moving monopterus albus Zuiew fries attached to roots of Pistias tratiotes out of the net cage for independent breeding in 15th to 30th days after bubbling and spawning of the monopterus albus Zuiew. According to the soilless propagation method disclosed by the invention, the area of a monopterus albus Zuiew propagation net cage is enlarged, the myriophyllum aquaticum is utilized to replace soil so as to be used as a monopterus albus Zuiew nest, the number of parent monopterus albus Zuiew in the net cage is increased, the pairing success rate of the parent monopterus albus Zuiew is greatly increased, and meanwhile, a parent stress reaction is relieved by utilizing rich roots of the myriophyllum aquaticum, so that the influence of adverse weather is cast off, the propagation frequency is increased, and the soilless propagation method has the characteristics of convenience in operation, wide application range, high germination quantity per unit area, high economic benefit and the like.

The invention provides a special additive for silo of composite rice straws and a preparation method thereof; the method comprises the following steps: 20g to 40g of fresh green plants is added with 100g to 200g of sterile distilled water, and the obtained mixture is ground and stirred fully in a mixing stirrer and is filtered with sterile gauze so as to obtain green sap; the green sap carries out anaerobic fermentation for 48 hours at the temperature of 30 DEG C, then the fermentation liquid is diluted by 20 times, vacuumized, cooled and dried and then the fermentation liquid of the green sap is obtained; and the fermentation liquid of the green sap and dehydrogenation sodium acetate are mixed according to the proportion of 100 : 1(V/W) so as to obtain the special additive for silo of composite rice straws of the invention. The product of the invention prepares the spray usage of silo additives prepared according to the water content of rice straws in the silo material (use spraying equipment), can realize silo of rice straws of the entire plant on a large scale, can guarantee that the quality of the silo can reach higher level more than one grade (evaluation standard for international silo quality) and improves the aerobic stability of the silo greatly so as to avoid the secondary fermentation loss of hollow rice straws.

The invention discloses a tissue culture method for Punica granatum and a culture medium. The culture method comprises explants picking, induction of differentiated seedlings, proliferation culture of differentiated seedlings, rooting culture of differentiated seedlings and transplantation of tissue culture seedlings to the field. The culture medium comprises an induction medium, a proliferation medium and a rooting medium. A Punica granatum tissue culture induction method is also included. The three mediums are respectively prepared and applied correspondingly. By the method of the invention, propagation coefficient of Punica granatum is raised; some insufficiencies of a traditional tissue culture technology are compensated; contamination rate of explants is reduced; and differentiation rate and transplanting survival rate of buds are enhanced. by the preparation method of the induction medium, the proliferation medium and the rooting medium, culture period of tissue culture seedlings can be shortened, there is no variation, and uniformity of seedlings is high. In addition, proliferation is rapid, propagation coefficient is raised. Rooting rate is high, there is no callus between roots and seedlings, and roots are all normal roots. By the cooperation of the three mediums, survival rate of domestication and proliferation coefficient can be greatly raised, and cost of tissue culture seedlings is reduced.

The invention discloses a method for establishing a paeonia lactiflora in-vitro regeneration system. The system is characterized by comprising the following steps: obtaining an explant, preparing callus, carrying out differentiation of adventitious buds, carrying out proliferation, carrying out root induction, carrying out seedling hardening, and carrying out transplanting. According the application, the in-vitro regeneration system of a paeonia lactiflora variety of paeoniae alba is systematically established, and the application is one of the important ways to solve the shortage of peony seedlings, has very important significance for the south domestication of the paeonia lactiflora and the development, promotion and utilization of the paeonia lactiflora, and has a wide application prospect.

The invention discloses a method for establishing a rachis in-vitro regeneration system of Heuchera micrantha variety tapestry. The method comprises following steps: (1), an explant is selected and disinfected; (2), after the treated rachis is cut, callus induction culture is performed; (3), callus is subjected to differentiation to induce adventitious buds; (4), the adventitious buds are subjected to subculture multiplication to culture seedlings; (5), test-tube plantlets obtained after subculture multiplication culture are subjected to rooting culture; (6), seedling hardening culture is performed. The Heuchera micrantha variety tapestry is subjected to tissue culture with the propagation method, the adventitious bud proliferation rate in one subculture period (35d) is 8 times or higher,the propagation speed is high, and the problem of seedling breeding is effectively solved.

The invention discloses a dahlia cultivation and reproduction method. The method comprises the following steps that 1, earth-nuts are selected, and germination is accelerated; 2, a seedbed is made; 3, normal buds are broke off and inserted in the seedbed, watering is conducted, the temperature and humidity are controlled, water is sprayed to preserve moisture at any time, and after the buds take roots and can absorb nutrients from a culture soil, cuttage and transplantation are conducted after twenty more days of growth of the buds; 4, enough amount of decomposed manure is applied to a plowed soil, restbalk surfaces are made according to view and admire needs, the restbalk surfaces are about 60 cm in width, furrows are about 40 cm in width, dahlia seedlings after rooting are transplanted into the restbalk surfaces, profound water is poured, the distances between the seedlings are 1 m, bamboo poles are inserted on the sides of the dahlia seedlings, seedling poles are connected to the bamboo poles, so that the stability of the seedlings is maintained, and the seedlings are prevented from being broke off; 5, water, fertilizer and cultivation management after transplantation are well conducted. According to the method, the reproduction output of the dahlia earth-nuts is significantly improved, a more extensive plantation can be achieved, and dahlias obtained through a cuttage method are more convenient for planting of landscape construction.

The invention discloses a tissue culture propagation method for Heuchera micrantha variety 'Obsidian'. The method comprises following steps: (1), an explant is selected and disinfected; (2), after treated rachis is cut, callus induction and culture are performed; (3), callus is subjected to differentiation for induction of adventitious buds; (4), the adventitious buds are subjected to subculture multiplication to form seedlings; (5), test-tube plantlets obtained after subculture multiplication are subjected to rooting culture; (6), seedling hardening culture is performed. The Heuchera micrantha variety 'Obsidian' is subjected to tissue culture with the propagation method, the adventitious bud proliferation rate in one subculture period (35d) is 10 times or higher, the propagation speed ishigh, and the problem about seedling breeding is effectively solved.

A tissue cultured seedling micrografting method for acer palmatum 'orange dream' is disclosed. The method includes (1) a step of culturing a sterile stock, namely a step of cutting an acer elegantulum branch segment with sprouts, performing tissue culture to obtain an acer elegantulum sterile seedling, and cutting the acer elegantulum sterile seedling in an inclined matter to obtain the sterile stock, (2) a step of culturing a sterile scion, namely a step of cutting an acer palmatum 'orange dream' branch segment with sprouts, performing tissue culture to obtain an acer palmatum 'orange dream' sterile seedling, and cutting the acer palmatum 'orange dream' sterile seedling in an inclined matter to obtain the sterile scion, (3) a step of tissue cultured seedling micrografting, namely a step of aligning the oblique section of the sterile stock and the oblique section of the sterile scion, fixing to obtain a micrografted seedling, inoculating the micrografted seedling to a medium and culturing, and (4) a step of seedling hardening and transplanting, namely a step of transferring the micrografted seedling obtained in the step (3) into a greenhouse, performing earlier-period seedling hardening, transferring into a seed bed, performing seedling hardening, performing mist spray treatment, sterilizing, and transplanting after the seedling hardening is completed. The method increases the grafting efficiency, increases the reproductive output, is high in survival rate and can be used for large-scale cultivation of small-size acer palmatum 'orange dream' seedlings.

The invention discloses a method for preparing newcastle disease and infectious bronchitis virus solution. The method specifically comprises the following steps: firstly, diluting newcastle disease virus seeds and infectious bronchitis virus seeds; inoculating newcastle disease virus seed diluent into an embryonated egg and after incubating for 24 hours, inoculating infectious bronchitis virus seed diluent into the same embryonated egg; and incubating at a temperature of 37 DEG C to obtain the newcastle disease and infectious bronchitis virus solution. According to the method disclosed by the invention, the mutual interface and restriction of two viruses in the inoculating and incubating process are effectively reduced, the mortality and elimination rate of the embryonated egg in the culturing process is reduced, the virus proliferation number is increased, the process is simplified, raw materials are saved and the production cost is reduced.

Belonging to plant cultivation technologies, the invention provides an in vitro culturing method for photinia fraseri breeding. The method comprises initiation culture, proliferation culture, and rooting culture. The method specifically consists of the steps of: initiating culture, inoculating a terminal bud or a stem segment with one axillary bud into an initiation culture medium, which is then placed in a culture room of 23-27DEG C, taking a fluorescent lamp as the light source, controlling the illumination intensity at 1500Lx-2000Lx, and conducting illumination for 10h/d and culturing for 20-30d, with the relative humidity of the culture room maintained at 70%-80%; transferring a generated adventitious bud into a proliferation culture medium, which is placed in a culture room of 23-27DEG C, taking a fluorescent lamp as the light source, controlling the illumination intensity at 1500Lx-2000Lx, and conducting illumination for 10h/d and culturing for 20-30d, with the relative humidity of the culture room maintained at 70%-80%, thus producing more adventitious buds, and transferring the robust young seedlings into a rooting culture medium, which is placed in a culture room of 20DEG C, taking a fluorescent lamp as the light source, controlling the illumination intensity at 1500Lx and the illumination time at 12h/d, maintaining the relative humidity of the culture room at 70%-80%, and carrying out rooting culture for 20-25d so as to make the length of the root up to 3-5cm.

The invention discloses a novel intelligent beekeeping box. The novel intelligent beekeeping box comprises an intelligent solar panel, a beehive cover, an intelligent temperature regulation panel, an inlet, a beehive panel, a first queen bee hive, a beehive partition board, a second queen bee hive, a ventilation port, a temperature controller, a fixing support, an intelligent controller, a state indication lamp and a switch, wherein the intelligent temperature regulation panel is mounted on the lower side of the beehive cover; the inlet is formed below the intelligent temperature regulation panel; the beehive panel is mounted on the left side of the inlet; the first queen bee hive is mounted on the right side of the beehive panel; the beehive partition board is arranged below the first queen bee hive; the outlet is formed below the beehive partition board; the second queen bee hive is mounted on the right side of the outlet; the temperature controller is mounted on the left side of the ventilation port; the intelligent controller is mounted on the right side of the fixing support; the switch is mounted on the right side of the intelligent controller; the state indication lamp is arranged above the switch. The device can be regulated according to operation requirements when used, can be operated more quickly, is more stable in overall structure and operates more stably.