76results about How to "Increase reproductive number" patented technology

The invention relates to the technical field of grape seedling cultivation, in particular to a grape seedling cultivating method. The grape seedling cultivating method includes the following steps that firstly, in early April, a grape seedling is cut into a grape shoot for cutting, wherein the length of the grape shoot for cutting ranges from 0.5 m to 0.8 m, and 4 to 6 bud eyes are reserved in the grape shoot for cutting, and then the grape shoot for cutting is disinfected through an ultraviolet sterilization lamp, wherein ultraviolet disinfection time ranges from 15 min to 30 min; secondly, a seedling bag filled with nutrient soil is placed on a seedbed, and the nutrient soil is completely irrigated through drop irrigation, wherein irrigation time ranges from 1 hour to 4 hours, the grape shoot for cutting is inserted into the nutrient soil after irrigation, and the bud eyes of the grape shoot for cutting are exposed out of the nutrient soil. According to the grape seedling cultivating method, seedling cultivation is conducted again in the seedling cultivation bag, nutrition supplied to the grape shoot for cutting is more sufficient, the grape shoot for cutting grows better, the excellent characters of a grape stock plant can be kept, the seedling reproduction number is increased by 50% in average compared with that of a root burying seedling cultivating method, and the yield obtained after fruiting is increased by 40% in average.

The invention discloses a method for building a leaf in vitro regeneration system of begonia rex. The method comprises the following steps of: (1) selecting an explant and sterilizing the explant; (2) carrying out adventitious bud induced cultivation after cutting the processed leaves; (3) carrying out high-voltage electrostatic field treatment; (4) carrying out subculture proliferation cultivation on the adventitious bud induced by the leaves; (5) carrying out rooting culturing on a test-tube plantlet after the subculture proliferation cultivation; and (6) cutting and rooting. The begonia rex is subjected to tissue culturing by adopting the propagation method; the reproduction rate of a culturing period (51d) is 243.1 times; the propagation speed is fast; and the problem of seeding propagation of the begonia rex is effectively solved.

The invention discloses a grape cuttage seedling method, which comprises the following steps of (1) soil preparation and ridge making; (2) rooting agent selection; (3) material selection and cuttage; and (4) management after cuttage and transplanting. When the cuttage seedling method provided by the invention is adopted, good properties of grape mother strains can be maintained, and the method has the advantages that the propagating factor is high, the plant growth is healthy, strong and regular, the operation is simple and convenient, and the like. The seedling propagating number is improved by more than 80 percent through being compared with that of a root burying seedling method, in addition, the popularization and the application are easy, the cost is low, the propagating period is short, and the seedling growth is fast.

The invention discloses a method which can control the blooming and the propagation of the spring dendrobium. Firstly, a plant growth regulator which is favorable for the growth of the side bud of the plant is selected, and is sprayed on the spring dendrobium plants by proper consistence and proper use method to accelerate the axillary bud to grow. When spraying the plant growth regulator, low temperature of 8-13 DEG C is matched; manure water, other circumstance condition, spray consistence and spray time are controlled, and then the axillary bud is induced to grow into a flower bud and then a flower. Or proper temperature and manure water are matched, and the sxillary bud is induced to grow into a high bud for propagation. The selected plant growth regulator is 6 benzyl aminopurine, the spray consistence is 100ppm to 500ppm; the spray method is leaf surface spray or irrigation. The invention can cause the gemma of the axillary bud of the spring dendrobium to sprout tidily, and can ensure the florescence to be unified, as well as can ensure the propagation efficiency to be high.

A medium-and-low temperature ground heat and biomass gas combined power generation system is characterized in that a working medium evaporator is connected with a working medium superheater, a prime motor and a power generator of a gas boiler, the prime motor is communicated with a working medium condenser and a working medium storage tank, and the working medium storage tank is communicated with a working medium preheater of the gas boiler through a working medium booster pump; a production well is communicated with a ground heat water processing device and the working medium evaporator, and the working medium evaporator is communicated with a second inner heating pipe and a first inner heating pipe; the first inner heating pipe is communicated with an inverted well, an underground heat storage part and the production well; biomass is communicated with an acidification hydrolysis tank and communicated with a biogas residue fermentation tank and biogas fertilizer through a biogas residue circulation pump, and the biogas residue fermentation tank is communicated with a gas storage tank through a first gas booster pump; the acidification hydrolysis tank is communicated with an anaerobic digestor through an organic acid circulation pump; the anaerobic digestor is communicated with the gas storage tank and a combustor in the gas boiler through a second gas booster pump; and an anaerobic digester filling module is arranged in the anaerobic digestor. A power generation cost calculating method for the medium-and-low temperature ground heat and biomass gas combined power generation system is provided.

The invention discloses a begonia masoniana leaf in-vitro regeneration system establishment method, which is characterized by comprising: selection and disinfection of explants, adventitious bud induction, adventitious bud subculture to obtain a test tube plantlet, root induction, and transplantation. According to the present invention, with the application of the breeding method to carry out tissue culture on the begonia masoniana, the one culture period proliferation rate is more than 1200 times, the breeding is fast, and the difficult problem of the begonia masoniana seedling breeding is effectively solved.

The invention discloses a cutting propagation method of mono maple in seedling bags and relates to a fast vegetative propagation of preferred individual plants of the mono maple. The method includes: firstly, subjecting branches of selected fine individual big trees to grafting to obtain nursery stocks, establishing a cutting orchard to obtain annual branches suitable for shoot cutting propagation; and secondly, performing cutting propagation by degradable seedling bags. By the cutting propagation method of mono maple in seedling bags, survival rate of shoot cuttings is increased, propagation amount of new varieties is increased greatly, propagation cycle is shortened and fine characteristics of new varieties of the mono maple are maintained. The cutting propagation method of mono maple in seedling bags is suitable for popularization of propagation technologies of new varieties.

The invention belongs to the field of agricultural technology, and in particular relates to a rapid breeding method for apple non-toxic dwarf self-root stock M9‑T337 stock. The method comprises the following concrete steps: construction of stock parent gardens; construction of breeding gardens; construction of demonstration gardens, and construction of modern apple cultivation demonstration gardens according to the standards of "four reforms, three transformations and three festivals". The invention adopts the layering technology of the same year of planting, shortens the time for seedlings to emerge from the nursery, and improves the breeding efficiency of seedlings. Adopt the method of flat stubble when the new seedling grows to 20cm, promote it to increase 2-5 new shoots, and when most of the new shoots grow to 30cm, 40cm, and 50cm on the seedling stem, press the decomposed sawdust 10cm / time in 3 times, and then Cultivate 5cm of soil to promote new roots of new stock seedlings. Compared with the foreign method of mixing soil with sawdust, it saves labor, time and effort. At the same time, this breeding method shortened the breeding time of non-toxic M9-T337 apple dwarf self-root stock by 50%, and increased the number of reproduction by more than 2 times.

The invention discloses a blueberry tissue culture method. The method includes the following steps that tender stems or stem tips of annual vegetative shoots are selected, leaves are cut off in a laboratory, and the stems or stem tips are sterilized and cut into stem segments which are 2 cm long; the sterilized stem segments are moved into wild-mouth bottles containing solid culture media, a sterile system is built, MS culture media are used, sealing is conducted, and the culture bottles are placed in a culture room to be cultured; by means of culture preservation, a sterile system of blueberries is basically built, and at this time, a subculture process starts; tissue culture seedlings obtained after subculture enter the rooting culture stage, and an in-bottle rooting mode is adopted; the rooted tissue culture seedlings are conveyed into a transitional greenhouse for transition by means of transition matrixes, during transplantation, the culture media on roots of the tissue culture seedlings are cleaned away, then the tissue culture seedlings are transplanted on the matrixes and covered with a small shed after transplantation, and transitional culture is conducted for 15-20 d. The rooting rate can reach 85% or above, and the survival rate of transplantation can reach 90% or above.

The invention discloses a method for cultivating sweet potato seedlings, and the planting time is from the middle ten days of November every year to the June the next year. The method comprises the following steps that (1) a ploughed field is ridged; (2) preprocessing is carried out on shorn sweet potato seedlings, the sweet potato seedlings on every two adjacent ridges are planted oppositely, and then the sweet potato seedlings are watered; (3) when the outdoor temperature is lower than or equal to15 DEG C, a tunnel is built between every two adjacent ridges with bamboo sticks, the tunnels are covered with films, and the films are removed when the outdoor temperature keeps at 25 DEG C or above. Through the method for cultivating sweet potato seedlings, the planting time of spring sweet potatoes is advanced by 20-30 d compared with a potato block seedling cultivation method, the breeding frequency of sweet potato seedlings is increased, yield of the sweet potato seedlings is increased, cultivation of new species is further facilitated, a large number of seedlings can be cultivated within a short time through the method, and the application and popularization speed is improved. In addition, the method can improve the survival and growth capacity of sweet potatoes at low temperature and further improve the utilization rate of land.

The invention discloses a soilless propagation method of monopterus albus Zuiew. The soilless propagation method comprises the following steps: setting an opened net cage which is not less than 15m<2> in area in a pond or a greenhouse, putting a pile of round myriophyllum aquaticum in the net cage every 1.5m, putting two parent monopterus albus Zuiew in each pile of the myriophyllum aquaticum, feeding mixed bait every 2 to 3 days, and moving monopterus albus Zuiew fries attached to roots of Pistias tratiotes out of the net cage for independent breeding in 15th to 30th days after bubbling and spawning of the monopterus albus Zuiew. According to the soilless propagation method disclosed by the invention, the area of a monopterus albus Zuiew propagation net cage is enlarged, the myriophyllum aquaticum is utilized to replace soil so as to be used as a monopterus albus Zuiew nest, the number of parent monopterus albus Zuiew in the net cage is increased, the pairing success rate of the parent monopterus albus Zuiew is greatly increased, and meanwhile, a parent stress reaction is relieved by utilizing rich roots of the myriophyllum aquaticum, so that the influence of adverse weather is cast off, the propagation frequency is increased, and the soilless propagation method has the characteristics of convenience in operation, wide application range, high germination quantity per unit area, high economic benefit and the like.

The invention relates to a secondary shearing method for de-viruses potato germchit which comprises transplanting the test tube seedling, employing the breeding techniques of shearing, cutting and enlarging toxic seeds sprout to the fitness sprouts. The disclosed method can diminish the complex procedure of indoor test tube sprout breeding, it is time- and labor-conserving.

The invention provides a special additive for silo of composite rice straws and a preparation method thereof; the method comprises the following steps: 20g to 40g of fresh green plants is added with 100g to 200g of sterile distilled water, and the obtained mixture is ground and stirred fully in a mixing stirrer and is filtered with sterile gauze so as to obtain green sap; the green sap carries out anaerobic fermentation for 48 hours at the temperature of 30 DEG C, then the fermentation liquid is diluted by 20 times, vacuumized, cooled and dried and then the fermentation liquid of the green sap is obtained; and the fermentation liquid of the green sap and dehydrogenation sodium acetate are mixed according to the proportion of 100 : 1(V / W) so as to obtain the special additive for silo of composite rice straws of the invention. The product of the invention prepares the spray usage of silo additives prepared according to the water content of rice straws in the silo material (use spraying equipment), can realize silo of rice straws of the entire plant on a large scale, can guarantee that the quality of the silo can reach higher level more than one grade (evaluation standard for international silo quality) and improves the aerobic stability of the silo greatly so as to avoid the secondary fermentation loss of hollow rice straws.

The invention discloses a tissue culture method for Punica granatum and a culture medium. The culture method comprises explants picking, induction of differentiated seedlings, proliferation culture of differentiated seedlings, rooting culture of differentiated seedlings and transplantation of tissue culture seedlings to the field. The culture medium comprises an induction medium, a proliferation medium and a rooting medium. A Punica granatum tissue culture induction method is also included. The three mediums are respectively prepared and applied correspondingly. By the method of the invention, propagation coefficient of Punica granatum is raised; some insufficiencies of a traditional tissue culture technology are compensated; contamination rate of explants is reduced; and differentiation rate and transplanting survival rate of buds are enhanced. by the preparation method of the induction medium, the proliferation medium and the rooting medium, culture period of tissue culture seedlings can be shortened, there is no variation, and uniformity of seedlings is high. In addition, proliferation is rapid, propagation coefficient is raised. Rooting rate is high, there is no callus between roots and seedlings, and roots are all normal roots. By the cooperation of the three mediums, survival rate of domestication and proliferation coefficient can be greatly raised, and cost of tissue culture seedlings is reduced.

A preparation method of a medium for rapid breeding of Photinia fraseri belongs to the technical field of plant cultivation and comprises steps of initial medium preparation, enrichment medium preparation and rooting medium preparation. The initial medium is prepared by using MS as a basal medium with the addition of 2.0mg / L of 6-BA, 0.15mg / L of NAA, 0.1% of active carbon, 3% of cane sugar and 0.65% of an agar powder at pH 5.8. The enrichment medium is prepared by using half of MS as a basal medium with the addition of 2.5mg / L of 6-BA, 0.5mg / L of NAA, 3% of cane sugar and 0.65% of an agar powder at pH 5.8. The rooting medium is prepared by using half of MS as a basal medium with the addition of 1.5mg / L of IBA, 0.2mg / L of NAA, 1.5% of cane sugar and 0.65% of an agar powder at pH 5.8. The preparation method provided by the invention can be used to greatly increase the breeding quantity, accelerate the breeding speed and reduce the cost.

The invention discloses a pear tree cutting seedling-raising method. The method comprises the steps that in the early morning in July, on the condition that the temperature is 20-25 DEG C and the humidity is 70-80%, branches with the length being 8-10 cm of a pear tree are cut down to be used as cutting slips, roots of the cutting slips are soaked and steeped into a potassium permanganate solution for 1-2 min, the roots of the cutting slips are inserted into a rooting agent to be soaked and steeped for 0.5-1 h and then taken out to be inserted into a cutting medium, and then light culture is performed for 45-55 d and then transplanting is performed. By adopting the cutting seedling-raising method, the good characters of pear tree stock plants are kept, and the pear tree cutting seedling-raising method has the advantages that the propagation coefficient is high, plants grow robustly and neatly, and operation is easy and convenient, the reproductive number of seedlings is increased by 60% or above compared with a root embedding seedling-raising method, the fruit yield is stable, and the quality is high.

The invention belongs to the technical field of plant cultivation, and relates to a rapid propagation method for cultivating a tissue culture seedling by utilizing a Mokara axillary bud tissue. The method comprises an axillary bud inducing process, a protocorm propagation process, a process of inducing cluster bud with protocorm, a strong seedling cultivation process, and a rooting cultivation process. The Mokara axillary bud tissue is used as an explant; and the Mokara seedling is rapidly bred by protocorm differentiation. Therefore, the propagation speed and the propagation quantity of the Mokara seedling are improved; a rapid propagation system of Mokara tissue culture is built; an effective path is provided for large-scale industrialized production of the Mokara seedling, and the rapid propagation method has great economic benefits.

The invention relates to the technical field of grape secondary fructifying and provides a grape secondary fructifying method. The grape secondary fructifying method is characterized by comprising the following steps of 1 cutting slips; 2 purchasing nursery stock bags; 3 performing irrigation before cutting; 4 performing cuttage; 5 performing management; 6 performing planting field management; 7 grape trellis erection. The grape secondary fructifying method can keep excellent characters of grape stock plants and has the advantages of being high in propagation coefficient, enabling the growing plants to be healthy, strong and order and being simple and convenient to operate and the like, the nursery stock propagation amount is averagely increased by 70% compared with a root burying and seedling cultivation method, the secondary fructifying yield is averagely improved by 30% compared with primary fructifying, plant diseases and insect pests do not easily occur, and secondary fruits are bright-colored and good in taste.

The invention provides a lactic acid bacteria fermentation medium for feed and a preparation method thereof, belonging to the technical field of microorganisms, wherein the fermentation medium comprises whey protein hydrolysate obtained by acid hydrolysis of whey protein with inulin. The preparation method of fermentation medium comprises: preparing whey protein hydrolysate: carrying out acid hydrolysis on whey protein added with inulin; and mixing the resulting whey protein hydrolysate, yeast powder, glucose, buffer salt, Mg<2+> water-soluble compound, Fe<2+> water-soluble compound, and MnSO4. As that fermentation medium of the invention can prevent the damage of oxygen to the feed lactic acid bacteria, more ATP is provided for the feeding lactic acid bacteria, the growth requirement ofthe fermentation medium for the feed lactic acid bacteria is prolonged, the acid stress resistance and the propagation quantity of the feeding lactic acid bacteria are improved, and high-density culture is realized. The whey protein hydrolysate prepared by the preparation method of the invention comprises D Type amino acids and flavoring amino acids.

The invention discloses a siraitia grosvenori seedling method and belongs to the technical field of seedling. The siraitia grosvenori seedling method includes the steps of 1), subjecting siraitia grosvenori cuttings to disinfection with ultraviolet rays; 2), inserting the cuttings in moist nutrient soil to have bud eyes exposed; 3), performing drop irrigation every 2-4 days, transplanting new buds to planting grooves after growing for 3-6 days and watering the new buds every 5-8 days; 4), setting a frame for the siraitia grosvenori after the cuttings grow to 1.2-1.5 meters, fixedly mounting seedling bags on the frame and reserving 3-6 holes, dipping rooting powder on the cuttings at the height corresponding to the seedling bags, allowing the cuttings to penetrate the seedling bags, filling the nutrient soil in the seedling bags, and spraying water on the nutrient soil in the seedling bags every 1-3 days till the new buds grow from the holes. With the method, problems that the survival rate of seedling propagation of the siraitia grosvenori is low resulting in the fact that seedling diseases and inset pests are serious and the yield of the siraitia grosvenori is low and the like can be effectively solved.

The invention discloses a method for establishing a paeonia lactiflora in-vitro regeneration system. The system is characterized by comprising the following steps: obtaining an explant, preparing callus, carrying out differentiation of adventitious buds, carrying out proliferation, carrying out root induction, carrying out seedling hardening, and carrying out transplanting. According the application, the in-vitro regeneration system of a paeonia lactiflora variety of paeoniae alba is systematically established, and the application is one of the important ways to solve the shortage of peony seedlings, has very important significance for the south domestication of the paeonia lactiflora and the development, promotion and utilization of the paeonia lactiflora, and has a wide application prospect.

The invention discloses a method for establishing a rachis in-vitro regeneration system of Heuchera micrantha variety tapestry. The method comprises following steps: (1), an explant is selected and disinfected; (2), after the treated rachis is cut, callus induction culture is performed; (3), callus is subjected to differentiation to induce adventitious buds; (4), the adventitious buds are subjected to subculture multiplication to culture seedlings; (5), test-tube plantlets obtained after subculture multiplication culture are subjected to rooting culture; (6), seedling hardening culture is performed. The Heuchera micrantha variety tapestry is subjected to tissue culture with the propagation method, the adventitious bud proliferation rate in one subculture period (35d) is 8 times or higher,the propagation speed is high, and the problem of seedling breeding is effectively solved.

The invention discloses a dahlia cultivation and reproduction method. The method comprises the following steps that 1, earth-nuts are selected, and germination is accelerated; 2, a seedbed is made; 3, normal buds are broke off and inserted in the seedbed, watering is conducted, the temperature and humidity are controlled, water is sprayed to preserve moisture at any time, and after the buds take roots and can absorb nutrients from a culture soil, cuttage and transplantation are conducted after twenty more days of growth of the buds; 4, enough amount of decomposed manure is applied to a plowed soil, restbalk surfaces are made according to view and admire needs, the restbalk surfaces are about 60 cm in width, furrows are about 40 cm in width, dahlia seedlings after rooting are transplanted into the restbalk surfaces, profound water is poured, the distances between the seedlings are 1 m, bamboo poles are inserted on the sides of the dahlia seedlings, seedling poles are connected to the bamboo poles, so that the stability of the seedlings is maintained, and the seedlings are prevented from being broke off; 5, water, fertilizer and cultivation management after transplantation are well conducted. According to the method, the reproduction output of the dahlia earth-nuts is significantly improved, a more extensive plantation can be achieved, and dahlias obtained through a cuttage method are more convenient for planting of landscape construction.

The invention discloses a tissue culture propagation method for Heuchera micrantha variety 'Obsidian'. The method comprises following steps: (1), an explant is selected and disinfected; (2), after treated rachis is cut, callus induction and culture are performed; (3), callus is subjected to differentiation for induction of adventitious buds; (4), the adventitious buds are subjected to subculture multiplication to form seedlings; (5), test-tube plantlets obtained after subculture multiplication are subjected to rooting culture; (6), seedling hardening culture is performed. The Heuchera micrantha variety 'Obsidian' is subjected to tissue culture with the propagation method, the adventitious bud proliferation rate in one subculture period (35d) is 10 times or higher, the propagation speed ishigh, and the problem about seedling breeding is effectively solved.

A tissue cultured seedling micrografting method for acer palmatum 'orange dream' is disclosed. The method includes (1) a step of culturing a sterile stock, namely a step of cutting an acer elegantulum branch segment with sprouts, performing tissue culture to obtain an acer elegantulum sterile seedling, and cutting the acer elegantulum sterile seedling in an inclined matter to obtain the sterile stock, (2) a step of culturing a sterile scion, namely a step of cutting an acer palmatum 'orange dream' branch segment with sprouts, performing tissue culture to obtain an acer palmatum 'orange dream' sterile seedling, and cutting the acer palmatum 'orange dream' sterile seedling in an inclined matter to obtain the sterile scion, (3) a step of tissue cultured seedling micrografting, namely a step of aligning the oblique section of the sterile stock and the oblique section of the sterile scion, fixing to obtain a micrografted seedling, inoculating the micrografted seedling to a medium and culturing, and (4) a step of seedling hardening and transplanting, namely a step of transferring the micrografted seedling obtained in the step (3) into a greenhouse, performing earlier-period seedling hardening, transferring into a seed bed, performing seedling hardening, performing mist spray treatment, sterilizing, and transplanting after the seedling hardening is completed. The method increases the grafting efficiency, increases the reproductive output, is high in survival rate and can be used for large-scale cultivation of small-size acer palmatum 'orange dream' seedlings.

The invention discloses a method for preparing newcastle disease and infectious bronchitis virus solution. The method specifically comprises the following steps: firstly, diluting newcastle disease virus seeds and infectious bronchitis virus seeds; inoculating newcastle disease virus seed diluent into an embryonated egg and after incubating for 24 hours, inoculating infectious bronchitis virus seed diluent into the same embryonated egg; and incubating at a temperature of 37 DEG C to obtain the newcastle disease and infectious bronchitis virus solution. According to the method disclosed by the invention, the mutual interface and restriction of two viruses in the inoculating and incubating process are effectively reduced, the mortality and elimination rate of the embryonated egg in the culturing process is reduced, the virus proliferation number is increased, the process is simplified, raw materials are saved and the production cost is reduced.

The invention provides a divalent vaccine for pigs, wherein, the active ingredients are porcine aftosa inactivation antigen and porcine reproductive and respiratory obstrucion syndrome inactivation antigen with the proportion of 1 to 0.6 - 1.5. The invention also provides a preparation method of the divalent vaccine for pigs, which comprises the processes that monolayer cell is cultured by a rolling bottle, the basic virus and the virus culture fluid are accessed, the Ph value of the culture fluid is regulated to be 7.4 plus or minus 0.2 by adopting an HEPES or NaHCO3 buffer system and the accession amount of the virus and the culture fluid are 300 to 800 ml / 15L per bottle; the virus fluid is collected after the cytopathic effect appears and the virus is inactivated after the potency of the virus is measured; 50V of adjuvant and the mixed inactivation antigen are emulsified according to the volume ratio of 1 to 1, and an 11 model white oil or Marcol 52 model oil and the mixed inactivation antigen are emulsified according to the volume ratio of 1 - 2.0 to 1 for the purpose of confecting the divalent vaccine for pigs. The divalent vaccine of the invention can be put into super-large scale production without restricting the use of virus strain, and can ensure the immune efficacy while the immune dosage is reduced.

The invention discloses a tissue culture propagation method for Heuchera micrantha variety 'Quiet Oasis'. The method comprises following steps: (1), an explant is selected and disinfected; (2), aftertreated rachis is cut, callus induction and culture are performed; (3), callus is subjected to differentiation for induction of adventitious buds; (4), the adventitious buds are subjected to subculture multiplication to form seedlings; (5), test-tube plantlets obtained after subculture multiplication are subjected to rooting culture; (6), seedling hardening culture is performed. The Heuchera micrantha variety 'Quiet Oasis' is subjected to tissue culture with the propagation method, the adventitious bud proliferation rate in one subculture period (35d) is 10 times or higher, the propagation speed is high, and the problem about seedling breeding is effectively solved.

Belonging to plant cultivation technologies, the invention provides an in vitro culturing method for photinia fraseri breeding. The method comprises initiation culture, proliferation culture, and rooting culture. The method specifically consists of the steps of: initiating culture, inoculating a terminal bud or a stem segment with one axillary bud into an initiation culture medium, which is then placed in a culture room of 23-27DEG C, taking a fluorescent lamp as the light source, controlling the illumination intensity at 1500Lx-2000Lx, and conducting illumination for 10h / d and culturing for 20-30d, with the relative humidity of the culture room maintained at 70%-80%; transferring a generated adventitious bud into a proliferation culture medium, which is placed in a culture room of 23-27DEG C, taking a fluorescent lamp as the light source, controlling the illumination intensity at 1500Lx-2000Lx, and conducting illumination for 10h / d and culturing for 20-30d, with the relative humidity of the culture room maintained at 70%-80%, thus producing more adventitious buds, and transferring the robust young seedlings into a rooting culture medium, which is placed in a culture room of 20DEG C, taking a fluorescent lamp as the light source, controlling the illumination intensity at 1500Lx and the illumination time at 12h / d, maintaining the relative humidity of the culture room at 70%-80%, and carrying out rooting culture for 20-25d so as to make the length of the root up to 3-5cm.

The invention discloses a novel intelligent beekeeping box. The novel intelligent beekeeping box comprises an intelligent solar panel, a beehive cover, an intelligent temperature regulation panel, an inlet, a beehive panel, a first queen bee hive, a beehive partition board, a second queen bee hive, a ventilation port, a temperature controller, a fixing support, an intelligent controller, a state indication lamp and a switch, wherein the intelligent temperature regulation panel is mounted on the lower side of the beehive cover; the inlet is formed below the intelligent temperature regulation panel; the beehive panel is mounted on the left side of the inlet; the first queen bee hive is mounted on the right side of the beehive panel; the beehive partition board is arranged below the first queen bee hive; the outlet is formed below the beehive partition board; the second queen bee hive is mounted on the right side of the outlet; the temperature controller is mounted on the left side of the ventilation port; the intelligent controller is mounted on the right side of the fixing support; the switch is mounted on the right side of the intelligent controller; the state indication lamp is arranged above the switch. The device can be regulated according to operation requirements when used, can be operated more quickly, is more stable in overall structure and operates more stably.