In vitro culturing method for photinia fraseri breeding

Abstract

Belonging to plant cultivation technologies, the invention provides an in vitro culturing method for photinia fraseri breeding. The method comprises initiation culture, proliferation culture, and rooting culture. The method specifically consists of the steps of: initiating culture, inoculating a terminal bud or a stem segment with one axillary bud into an initiation culture medium, which is then placed in a culture room of 23-27DEG C, taking a fluorescent lamp as the light source, controlling the illumination intensity at 1500Lx-2000Lx, and conducting illumination for 10h/d and culturing for 20-30d, with the relative humidity of the culture room maintained at 70%-80%; transferring a generated adventitious bud into a proliferation culture medium, which is placed in a culture room of 23-27DEG C, taking a fluorescent lamp as the light source, controlling the illumination intensity at 1500Lx-2000Lx, and conducting illumination for 10h/d and culturing for 20-30d, with the relative humidity of the culture room maintained at 70%-80%, thus producing more adventitious buds, and transferring the robust young seedlings into a rooting culture medium, which is placed in a culture room of 20DEG C, taking a fluorescent lamp as the light source, controlling the illumination intensity at 1500Lx and the illumination time at 12h/d, maintaining the relative humidity of the culture room at 70%-80%, and carrying out rooting culture for 20-25d so as to make the length of the root up to 3-5cm.

Description

technical field [0001] The invention belongs to the field of asexual propagation of trees, and in particular relates to an in vitro cultivation method for the propagation of Photinia fruticosa. Background technique [0002] Photinia fragrans is mostly propagated by cuttings in production. Cuttages generally use branches as propagation materials. The traditional method is to select thick lignified or semi-lignified branches of the year, and cut cuttings about 8-15cm long. The incision is smooth and the upper incision distance The terminal bud is about 1cm, cut off half of the leaves on the top of the strip, and cut off the rest of the leaves. The base of the cut cuttings is treated with a rooting agent and inserted into the substrate to wait for rooting. [0003] First, this method needs a large amount of propagation materials, second, it has a great influence on the growth of the mother tree that is taken, and is unfavorable for tree shape cultivation. Third, it is time-cons...

AI Technical Summary

Problems solved by technology

[0003] First, this method needs a large amount of propagation materials. Second, it has a great impact on the growth of the mother tree that is taken from the material, which is not conducive to the cultivation of the tree shape. Third, when cutting the cuttings, pay attention to keeping the upper incision at a distance of 1 cm from the top bud, which is time-consuming and labor-intensive. Fourth, The reproduction coefficient is low, generally 30% in foreign countries, but only 20% in China