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Method for preparing newcastle disease and infectious bronchitis virus solution

A chicken infectious and bronchitis technology, applied in the direction of antiviral agents, viral antigen components, medical preparations containing active ingredients, etc. Increase virus titer, improve product quality, and reduce the effect of mutual interference

Inactive Publication Date: 2012-07-18
TIANJIN RINGPU BIO TECH
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  • Abstract
  • Description
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Problems solved by technology

[0010] However, because the growth of chicken infectious bronchitis virus can obviously inhibit the reproduction of chicken Newcastle disease virus, at the same time, there is mutual interference between the two in the breeding process
In actual production and application, if homeomorphic multiplication is inoculated in one step at the same time, it will inevitably cause unsatisfactory reproduction of IBV and NDV, affecting the quality and immune effect of the vaccine

Method used

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  • Method for preparing newcastle disease and infectious bronchitis virus solution

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Comparison scheme
Effect test

Embodiment 1

[0031] Comparison of Two Methods for Preparation of Newcastle Disease-Chicken Infectious Bronchitis Virus Liquid

[0032] 1. Two-step inoculation test method of homeomorphic double virus

[0033] 1.1 Virus seed dilution

[0034] Chicken Newcastle disease virus seed and chicken infectious bronchitis virus seed are diluted with sterilized physiological saline according to the method according to the regulations, and the virus seed dilution is obtained, wherein chicken infectious bronchitis virus seed (IB M41) is used as 10 -3 Double dilution, chicken Newcastle disease virus (ND La Sota) as 10 -4 double dilution.

[0035] 1.2 Inoculation

[0036] A total of 2500 pieces of 10-day-old SPF chicken embryos were taken, and 5 batches of parallel experiments were carried out, and 500 chicken embryos were injected into each batch.

[0037] Inoculate each embryo with 0.1ml of chicken Newcastle disease virus dilution, and incubate at 37°C for 24 hours, then inoculate chicken embryos wi...

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Abstract

The invention discloses a method for preparing newcastle disease and infectious bronchitis virus solution. The method specifically comprises the following steps: firstly, diluting newcastle disease virus seeds and infectious bronchitis virus seeds; inoculating newcastle disease virus seed diluent into an embryonated egg and after incubating for 24 hours, inoculating infectious bronchitis virus seed diluent into the same embryonated egg; and incubating at a temperature of 37 DEG C to obtain the newcastle disease and infectious bronchitis virus solution. According to the method disclosed by the invention, the mutual interface and restriction of two viruses in the inoculating and incubating process are effectively reduced, the mortality and elimination rate of the embryonated egg in the culturing process is reduced, the virus proliferation number is increased, the process is simplified, raw materials are saved and the production cost is reduced.

Description

technical field [0001] The invention relates to a method for preparing a virus liquid for poultry vaccines, in particular to a method for preparing a chicken Newcastle disease-chicken infectious bronchitis virus liquid. Background technique [0002] Newcastle disease (ND) and infectious bronchitis (IB) are still the main infectious diseases that endanger the poultry industry in my country. They have the characteristics of rapid spread and great harm, and can cause acute high-level contact diseases. After the poultry industry tends to be intensive and industrialized with high-density feeding methods, in order to prevent the occurrence of Newcastle disease and infectious bronchitis, the requirements for vaccines are also higher. The prevention and control of these two diseases has caused great concern in the industry. Pay attention to. [0003] Newcastle disease (ND) is an acute and highly contagious infectious disease of poultry caused by Newcastle disease virus (NDV). The ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/295A61K39/215A61K39/17A61P31/14
Inventor 刘建陈利红杨旭黄娜
Owner TIANJIN RINGPU BIO TECH
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