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Rapid propagation method for cultivating tissue culture seedling by utilizing Mokara axillary bud tissue

A technology of tissue culture and tissue culture seedlings, which is applied in the field of plant cultivation, can solve the problems of less plant tissue culture, achieve great economic benefits, and increase the effect of reproduction speed and reproduction number

Inactive Publication Date: 2013-03-20
三亚柏盈热带兰花产业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few research reports on the tissue culture of Mohs orchid in China, and most of the research on Mohs orchid abroad is on the study of its flowers, and there are relatively few reports on the tissue culture of plants.

Method used

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  • Rapid propagation method for cultivating tissue culture seedling by utilizing Mokara axillary bud tissue
  • Rapid propagation method for cultivating tissue culture seedling by utilizing Mokara axillary bud tissue
  • Rapid propagation method for cultivating tissue culture seedling by utilizing Mokara axillary bud tissue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1. Induction of axillary buds: Cut off 30 cm from the top of the stem of Mohs orchid, peel off all the leaves to expose the stem, take the axillary buds on each node as explants, disinfect with 75% alcohol for 30 s, wash with water 1-2 times, and place in the Disinfect with 2% sodium hypochlorite solution for 15 minutes, rinse with water for 3 to 4 times, and finally disinfect with 2% sodium hypochlorite solution for 3 minutes, rinse with water for 3 to 4 times, shake the triangular flask intermittently during the whole disinfection process, so that the explants can fully contact Disinfectant, so as to achieve better disinfection effect. Cut the sterilized stem section into 4cm stem section with at least 2 axillary buds in the middle, then carefully remove the axillary buds on the stem section without damaging the growth point, and inoculate it on the induction medium for cultivation. Each bottle of medium is inoculated with 2 After about 15 days of induction culture, t...

Embodiment 2

[0042] The implementation steps and the transplanting method of tissue culture bottle seedlings are the same as in Example 1, and the results are shown in Table 1 and Table 2. The basal medium and the medium of each stage are respectively:

[0043] Basal medium: ①macroelements: KNO 3 685mg / L; KH 2 PO 4 200mg / L; (Ca) 3 PO 4 180 mg / L; MgSO 4 ·7H 2 O 300 mg / L; (NH) 2 SO 4 400mg / L; ②Trace elements: FeSO 4 ·7H 2 O 22.0mg / L; Na 2 -EDTA 31.0 mg / L; MnSO 4 ·H 2 O 11.0mg / L; ZnSO 4 ·7H 2 O2.0mg / L; H 3 BO 3 1.5mg / L; ③ inositol 150mg / L; ④ sugar 15g / L; ⑤ agar powder 5.0g / L; pH adjusted to 5.4.

[0044] The induction medium is based on the basal medium with coconut water 150ml / L, a-naphthaleneacetic acid 0.3mg / L, 6-benzylaminopurine 3.5mg / L, and adenine 2mg / L.

[0045] Proliferation medium is based on the base medium with coconut water 130ml / L, peptone 1.5g / L, a-naphthaleneacetic acid 0.2mg / L, 6-benzylaminopurine 3.0mg / L, adenine 2.5mg / L.

[0046] Cluster bud inducti...

Embodiment 3

[0050] The implementation steps and the transplanting method of tissue culture bottle seedlings are the same as in Example 1, and the results are shown in Table 1 and Table 2. The basal medium and the medium of each stage are respectively:

[0051] Basal medium: ①macroelements: KNO 3 890mg / L; KH 2 PO 4 170mg / L; (Ca) 3 PO 4 250 mg / L; MgSO 4 ·7H 2 O 350 mg / L; (NH) 2 SO 4 700mg / L; ②Trace elements: FeSO 4 ·7H 2 O 20.0mg / L; Na 2 -EDTA 28.0 mg / L; MnSO 4 ·H 2 O 8.0mg / L; ZnSO 4 ·7H 2 O2.5mg / L; H 3 BO 3 2.0mg / L; ③ inositol 200mg / L; ④ sugar 16g / L; ⑤ agar powder 4.8g / L; pH adjusted to 5.3.

[0052] The induction medium is based on the basal medium by adding 180ml / L of coconut milk, 0.2mg / L of a-naphthaleneacetic acid, 4.0mg / L of 6-benzylaminopurine, and 1.5mg / L of adenine.

[0053] Proliferation medium is based on the base medium with coconut water 160ml / L, peptone 1.2g / L, a-naphthaleneacetic acid 0.3mg / L, 6-benzylaminopurine 2.0mg / L, adenine 2.0mg / L.

[0054] Cl...

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Abstract

The invention belongs to the technical field of plant cultivation, and relates to a rapid propagation method for cultivating a tissue culture seedling by utilizing a Mokara axillary bud tissue. The method comprises an axillary bud inducing process, a protocorm propagation process, a process of inducing cluster bud with protocorm, a strong seedling cultivation process, and a rooting cultivation process. The Mokara axillary bud tissue is used as an explant; and the Mokara seedling is rapidly bred by protocorm differentiation. Therefore, the propagation speed and the propagation quantity of the Mokara seedling are improved; a rapid propagation system of Mokara tissue culture is built; an effective path is provided for large-scale industrialized production of the Mokara seedling, and the rapid propagation method has great economic benefits.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and relates to a method for propagating tissue-cultured seedlings (test-tube seedlings) in plant biotechnology, in particular to a rapid propagation method for culturing tissue-cultured seedlings by utilizing Morse blue axillary bud tissue. Background technique [0002] Mokara is a new type of orchid hybridized by Singapore senior orchid breeder Hou Weili with three genera of Vanda, Quiquila and Spider Orchid. In the past ten years, Mohs orchid has been popular all over the world with its unique flower appearance. The planting area and market demand have increased year by year, and it has become one of the main cut orchid varieties. At present, the propagation method of Mohs orchid mainly adopts the traditional single-stem cutting-off propagation method, which is to take Mohs orchid plants that have grown for more than 3 to 4 years, and the plants are strong and the roots are healthy an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 刑孔惠孙崇格
Owner 三亚柏盈热带兰花产业有限公司
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