Rapid propagation method for cultivating tissue culture seedling by utilizing Mokara axillary bud tissue
A technology of tissue culture and tissue culture seedlings, which is applied in the field of plant cultivation, can solve the problems of less plant tissue culture, achieve great economic benefits, and increase the effect of reproduction speed and reproduction number
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Embodiment 1
[0030] 1. Induction of axillary buds: Cut off 30 cm from the top of the stem of Mohs orchid, peel off all the leaves to expose the stem, take the axillary buds on each node as explants, disinfect with 75% alcohol for 30 s, wash with water 1-2 times, and place in the Disinfect with 2% sodium hypochlorite solution for 15 minutes, rinse with water for 3 to 4 times, and finally disinfect with 2% sodium hypochlorite solution for 3 minutes, rinse with water for 3 to 4 times, shake the triangular flask intermittently during the whole disinfection process, so that the explants can fully contact Disinfectant, so as to achieve better disinfection effect. Cut the sterilized stem section into 4cm stem section with at least 2 axillary buds in the middle, then carefully remove the axillary buds on the stem section without damaging the growth point, and inoculate it on the induction medium for cultivation. Each bottle of medium is inoculated with 2 After about 15 days of induction culture, t...
Embodiment 2
[0042] The implementation steps and the transplanting method of tissue culture bottle seedlings are the same as in Example 1, and the results are shown in Table 1 and Table 2. The basal medium and the medium of each stage are respectively:
[0043] Basal medium: ①macroelements: KNO 3 685mg / L; KH 2 PO 4 200mg / L; (Ca) 3 PO 4 180 mg / L; MgSO 4 ·7H 2 O 300 mg / L; (NH) 2 SO 4 400mg / L; ②Trace elements: FeSO 4 ·7H 2 O 22.0mg / L; Na 2 -EDTA 31.0 mg / L; MnSO 4 ·H 2 O 11.0mg / L; ZnSO 4 ·7H 2 O2.0mg / L; H 3 BO 3 1.5mg / L; ③ inositol 150mg / L; ④ sugar 15g / L; ⑤ agar powder 5.0g / L; pH adjusted to 5.4.
[0044] The induction medium is based on the basal medium with coconut water 150ml / L, a-naphthaleneacetic acid 0.3mg / L, 6-benzylaminopurine 3.5mg / L, and adenine 2mg / L.
[0045] Proliferation medium is based on the base medium with coconut water 130ml / L, peptone 1.5g / L, a-naphthaleneacetic acid 0.2mg / L, 6-benzylaminopurine 3.0mg / L, adenine 2.5mg / L.
[0046] Cluster bud inducti...
Embodiment 3
[0050] The implementation steps and the transplanting method of tissue culture bottle seedlings are the same as in Example 1, and the results are shown in Table 1 and Table 2. The basal medium and the medium of each stage are respectively:
[0051] Basal medium: ①macroelements: KNO 3 890mg / L; KH 2 PO 4 170mg / L; (Ca) 3 PO 4 250 mg / L; MgSO 4 ·7H 2 O 350 mg / L; (NH) 2 SO 4 700mg / L; ②Trace elements: FeSO 4 ·7H 2 O 20.0mg / L; Na 2 -EDTA 28.0 mg / L; MnSO 4 ·H 2 O 8.0mg / L; ZnSO 4 ·7H 2 O2.5mg / L; H 3 BO 3 2.0mg / L; ③ inositol 200mg / L; ④ sugar 16g / L; ⑤ agar powder 4.8g / L; pH adjusted to 5.3.
[0052] The induction medium is based on the basal medium by adding 180ml / L of coconut milk, 0.2mg / L of a-naphthaleneacetic acid, 4.0mg / L of 6-benzylaminopurine, and 1.5mg / L of adenine.
[0053] Proliferation medium is based on the base medium with coconut water 160ml / L, peptone 1.2g / L, a-naphthaleneacetic acid 0.3mg / L, 6-benzylaminopurine 2.0mg / L, adenine 2.0mg / L.
[0054] Cl...
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