Tissue culture rapid-propagation method of fire axillary bud of North American crabapple tableland
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A tissue culture rapid propagation and plateau technology, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of restricting popularization of cultivation and inability to carry out large-scale propagation, and achieve good practicability, speed up propagation and effect of scale
Active Publication Date: 2015-05-06
江苏青好景观园艺有限公司
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Due to the introduction of plateau fire from abroad, there are few seeds, and most of them are propagated by grafting and cuttings. However, due to the limitation of seasons, scions and cuttings, large-scale propagation cannot be carried out, thus limiting the popularization and cultivation of this variety.
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Embodiment 1
[0023] A rapid propagation method of plateau fire Begonia tissue culture, comprising the following steps:
[0024] 1) Selection and pretreatment of explants: In April, select tender branches about 5 cm long from the mother plant of Plateau Fire Begonia, soak them in soapy water for 10 minutes, brush off surface stains with a soft brush, rinse them with running water for 2 hours, and set aside .
[0025] 2) Disinfection of explants: Soak the explants in 75% alcohol for 30 seconds on the ultra-clean workbench, rinse them with sterile water for 3 times, and then disinfect them with 0.1% mercury liter for 9 minutes. Bacterial water was washed 4 times, and the surface moisture was blotted dry with sterile filter paper for later use.
[0026] 3) Primary culture: After the explants were sterilized, they were cut into about 2 cm stem segments with one axillary bud, and inoculated on the primary medium for cultivation ( figure 1 ). The temperature of the culture room is 25±2°C, the ...
Embodiment 2
[0032] A rapid propagation method of plateau fire Begonia tissue culture, comprising the following steps:
[0033] 1) Selection and pretreatment of explants: In April, select tender branches about 5 cm long from the mother plant of Plateau Fire Begonia, soak them in soapy water for 10 minutes, brush off surface stains with a soft brush, rinse them with running water for 2 hours, and set aside .
[0034] 2) Disinfection of explants: Soak the explants in 75% alcohol for 30 seconds on the ultra-clean workbench, rinse them with sterile water for 4 times, and then disinfect them with 0.1% mercury liter for 9 minutes. Wash with sterile water for 5 times, and blot the surface moisture with sterile filter paper for later use.
[0035] 3) Primary culture: After the explants were sterilized, they were cut into about 2 cm stem segments with one axillary bud, and inoculated on the primary medium for cultivation ( figure 1 ). The temperature of the culture room is 25±2°C, the light time...
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Abstract
The invention discloses a tissue culture rapid-propagation method of a fire axillary bud of a North American crabapple tableland. The tissue culture rapid-propagation method comprises the following steps: taking an axillary bud on a spring twig of a fire crabapple of the North American crabapple tableland as an explant, disinfecting and inoculating, then culturing on an initial culture medium till the axillary bud is germinated, and then transferring the explant to a proliferation culture medium for proliferation culture; when a split bud grows to 2-3 cm, transferring to a rooting culture medium for rooting induction to form a complete plant; carrying out seedling adaptation, domestication and transplantation survival on a plantlet to obtain an expanding propagation seedling. The tissue culture rapid-propagation method disclosed by the invention can be used for inducing the axillary bud of the fire crabapple of the North American crabapple tableland by adopting a tissue culture technology and establishing an in-vitro rapid propagation technical system of an axillary bud regenerated plant, thereby keeping the excellent variety and providing the seedlings on a large scale; in addition, the tissue culture rapid-propagation method disclosed by the invention achieves the proliferation rate of more than 340%, the plantlet rooting rate of more than 65% and the survival rate of more than 80%, can be used for accelerating the propagation speed and scale of the fire North American crabapple and has very good practicability.
Description
technical field [0001] The invention relates to the technical field of crabapple tissue culture, in particular to a method for tissue culture and rapid propagation of fire axillary buds of North American crabapple plateau. Background technique [0002] Begonia Plateau Fire in North America is a small deciduous tree of the Rosaceae apple genus introduced from North America. The new leaves are bright wine red, and the mature leaves are olive green. In late autumn, the leaves gradually change from brown green to wine red. The flowers bloom in late April, and the flowering period is about two weeks. The buds are red and the petals are deep purple. The fruit is lantern-shaped, purple-red, hanging, and the bright red fruit in autumn can bear fruit until January of the next year, with a large amount of fruit. This variety has strong disease resistance, drought resistance and cold resistance, and can be planted in a wide range. It can be used as a potted flower or as a street tree....
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