Tissue culture rapid-propagation method of fire axillary bud of North American crabapple tableland

Abstract

The invention discloses a tissue culture rapid-propagation method of a fire axillary bud of a North American crabapple tableland. The tissue culture rapid-propagation method comprises the following steps: taking an axillary bud on a spring twig of a fire crabapple of the North American crabapple tableland as an explant, disinfecting and inoculating, then culturing on an initial culture medium till the axillary bud is germinated, and then transferring the explant to a proliferation culture medium for proliferation culture; when a split bud grows to 2-3 cm, transferring to a rooting culture medium for rooting induction to form a complete plant; carrying out seedling adaptation, domestication and transplantation survival on a plantlet to obtain an expanding propagation seedling. The tissue culture rapid-propagation method disclosed by the invention can be used for inducing the axillary bud of the fire crabapple of the North American crabapple tableland by adopting a tissue culture technology and establishing an in-vitro rapid propagation technical system of an axillary bud regenerated plant, thereby keeping the excellent variety and providing the seedlings on a large scale; in addition, the tissue culture rapid-propagation method disclosed by the invention achieves the proliferation rate of more than 340%, the plantlet rooting rate of more than 65% and the survival rate of more than 80%, can be used for accelerating the propagation speed and scale of the fire North American crabapple and has very good practicability.

Description

technical field [0001] The invention relates to the technical field of crabapple tissue culture, in particular to a method for tissue culture and rapid propagation of fire axillary buds of North American crabapple plateau. Background technique [0002] Begonia Plateau Fire in North America is a small deciduous tree of the Rosaceae apple genus introduced from North America. The new leaves are bright wine red, and the mature leaves are olive green. In late autumn, the leaves gradually change from brown green to wine red. The flowers bloom in late April, and the flowering period is about two weeks. The buds are red and the petals are deep purple. The fruit is lantern-shaped, purple-red, hanging, and the bright red fruit in autumn can bear fruit until January of the next year, with a large amount of fruit. This variety has strong disease resistance, drought resistance and cold resistance, and can be planted in a wide range. It can be used as a potted flower or as a street tree....

AI Technical Summary

Problems solved by technology

Due to the introduction of plateau fire from abroad, there are few seeds, and most of them are propagated by grafting and cuttings. However, due to the limitation of seasons, scions and cuttings, large-scale propagation cannot be carried out, thus limiting the popularization and cultivation of this variety.

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