Seed germination and rapid propagation method for cymbidium orchid

Abstract

The invention discloses a seed germination and rapid propagation method for cymbidium orchid, and relates to the technical field of plant tissue culture. The method comprises the steps that culturing is conducted through a seed germination culture medium and then conducted through a protocorm seedling differentiation culture medium, wherein the seed germination culture medium is a VW culture medium containing 6-benzyl adenine with the concentration of 0.5 mg / L to 1.0 mg / L, and the protocorm seedling differentiation culture medium is a KC culture medium containing banana juice with the mass fraction of 10%. According to the method, the seed germination and propagation speed of the cymbidium orchid can be significantly increased, the method is especially suitable for Zijingxiangyun cymbidium orchid and Laoranzi varieties difficult to germinate and culture, the culture time can be shortened, the culture technology is simple, conditions are created for commercialized production of the cymbidium orchid, and the orchid market demand exceeding supply is met.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a Cymbidium seed germination rapid propagation method, which achieves the purpose of rapidly producing seedlings through in vitro germination of orchid germplasm resource seeds. Background technique [0002] Orchid, a traditional famous flower in China, is rich in germplasm resources, widely distributed, and has high ornamental value. Its propagation method is generally branch propagation, which can only be carried out once every 3-4 years, and the propagation speed is relatively slow, which is difficult to meet the needs of the market. Orchid seeds are tiny, but they are surprisingly numerous, with millions of seeds in a single capsule. The number of orchid seeds is large, but because orchid seeds are very small and powdery, their structure can only be seen clearly under a microscope. It has no endosperm, only a simple embryo, surrounded by a loose, transparent, i...

AI Technical Summary

Problems solved by technology

At present, there have been successful cases of in vitro germination of other orchids, but orchids have strict requirements on germination conditions, especially Cymbidium, which is particularly sensitive to the components of the medium, hormone types, culture conditions, and culture time. The existing ones adapted to Cymbidium There are few schemes for Cymbidium, the patent title is "a medium composition suitable for aseptic germination of Cymbidium seeds and its method", and the patent application number "200910101739.0" discloses a technical scheme for cultivating Cymbidium using improved MS medium , but still have the disadvantages of low germination rate and slow reproduction speed, which cannot meet the needs of large-scale production

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