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Blueberry tissue culture method

A tissue culture and blueberry technology, which is applied in horticultural methods, botanical equipment and methods, cultivation and other directions, can solve the problems of low production efficiency, high cost, low reproduction coefficient, etc., and achieves saving production cost, promoting the formation and increasing reproduction. effect of quantity

Inactive Publication Date: 2017-01-04
云南省农业科学院园艺作物研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In the existing blueberry tissue culture technology, a single medium is generally used. An important problem in this technical method is that the reproduction coefficient is low. According to the difference in the concentration of hormones in the medium, the reproduction coefficient is generally between 3 and 12. , due to the low reproduction coefficient, it leads to problems such as low production efficiency and high cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A kind of blueberry tissue culture method, comprises the following steps:

[0038] 1) Explant treatment:

[0039] Select the young stems or tips of the vegetative branches of the current year, cut off the leaves in the laboratory, clean the dust with distilled water, and disinfect the stems or tips with 75% alcohol for 30 seconds , and then sterilized with 0.1% mercuric chloride for 20 to 60 minutes (the time is determined according to the degree of maturity of the explants, the more tender branches or buds are disinfected, the shorter the disinfection time), after the disinfection, the stem segment or shoot tip Cut into stems of about 2cm for later use;

[0040] 2) Aseptic system establishment:

[0041] Transfer the sterilized stems into a wide-mouthed bottle with solid medium. MS medium is used for the establishment of an aseptic system. The amount of medium in each bottle is 30-40ml. 10, then sealed, and then put the culture bottle into the culture room for cultivat...

Embodiment 2

[0049] A kind of blueberry tissue culture method, comprises the following steps:

[0050] 1) Explant treatment:

[0051] Select the young stems or tips of the vegetative branches of the current year, cut off the leaves in the laboratory, clean the dust with distilled water, disinfect the stems or tips with 75% alcohol for 30 seconds, and then use 0.15% mercuric chloride disinfection for 20 minutes (the time is determined according to the degree of maturity of the explant, and the more tender branches or buds are disinfected, the shorter the disinfection time), after the disinfection, cut the stem segment or stem tip into a stem segment of about 2 cm for use;

[0052] 2) Aseptic system establishment:

[0053] The sterilized stem section is moved into the wide-mouthed bottle with solid medium, the MS medium used for the establishment of the sterile line, the quantity of every bottle of culture medium is 30ml, and the blueberry stem sections or stem tips that are moved into each...

Embodiment 3

[0061] A kind of blueberry tissue culture method, comprises the following steps:

[0062] 1) Explant treatment:

[0063] Select the young stems or tips of the vegetative branches of the current year, cut off the leaves in the laboratory, clean the dust with distilled water, disinfect the stems or tips with 75% alcohol for 30 seconds, and then use 0.15% mercuric chloride disinfection for 60 minutes (the time is determined according to the degree of maturity of the explant, and the more tender branches or buds are disinfected, the shorter the disinfection time), after the disinfection, cut the stem segment or stem tip into a stem segment of about 2 cm for use;

[0064] 2) Aseptic system establishment:

[0065] The stem section after the disinfection is moved into the wide-mouthed bottle that is placed with solid medium, the MS medium that aseptic system is established, the quantity of every bottle of culture medium is 40ml, and the blueberry stem section or stem tip that every ...

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Abstract

The invention discloses a blueberry tissue culture method. The method includes the following steps that tender stems or stem tips of annual vegetative shoots are selected, leaves are cut off in a laboratory, and the stems or stem tips are sterilized and cut into stem segments which are 2 cm long; the sterilized stem segments are moved into wild-mouth bottles containing solid culture media, a sterile system is built, MS culture media are used, sealing is conducted, and the culture bottles are placed in a culture room to be cultured; by means of culture preservation, a sterile system of blueberries is basically built, and at this time, a subculture process starts; tissue culture seedlings obtained after subculture enter the rooting culture stage, and an in-bottle rooting mode is adopted; the rooted tissue culture seedlings are conveyed into a transitional greenhouse for transition by means of transition matrixes, during transplantation, the culture media on roots of the tissue culture seedlings are cleaned away, then the tissue culture seedlings are transplanted on the matrixes and covered with a small shed after transplantation, and transitional culture is conducted for 15-20 d. The rooting rate can reach 85% or above, and the survival rate of transplantation can reach 90% or above.

Description

technical field [0001] The invention belongs to the field of plant cultivation, and in particular relates to a blueberry tissue culture method. Background technique [0002] Blueberry, also known as lingonberry, is a shrub or small tree of the genus Vaccinium (Vaccinium) of the Vaccinium family. Blueberry belongs to the genus Vaccinium (Vaccinium spp.) General term for green shrubs. [0003] Blueberries are extremely rich in nutritional value, with an average soluble solid content of about 12%. In addition to sugar and acid common to conventional fruits, they also contain a large number of substances that are not available in other fruits that are beneficial to human health, such as: a large amount of water-soluble phenols There are as many as 15 kinds of pigments. Pharmacological studies have found that the total anthocyanins of blueberry have various functions such as promoting the resynthesis of retinoids on the human retina, improving circulation, anti-ulcer, and anti-i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00C05G1/00
CPCA01G24/00A01G31/00A01H4/001A01H4/008C05D9/00C05F11/02
Inventor 李坤明陈瑶王蜀陈伟胡忠荣
Owner 云南省农业科学院园艺作物研究所
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