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Method for establishing rachis in-vitro regeneration system of Heuchera micrantha variety tapestry

An in vitro regeneration, inflorescence rachis technology, applied in the direction of plant regeneration, horticultural methods, botanical equipment and methods, etc., can solve the problems of high pollution rate, difficulty in establishing sterile system, easy to form browning, etc.

Inactive Publication Date: 2019-05-17
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

The tissue culture rapid propagation system is limited by the explants of the female parent. The contamination rate of the explants is too high after simple disinfection, and browning is easy to occur after excessive disinfection. It is difficult to establish a sterile line

Method used

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  • Method for establishing rachis in-vitro regeneration system of Heuchera micrantha variety tapestry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0019] Explant Screening Example:

[0020] 1. Screening of the placement mode of explants in the culture medium

[0021] For the inflorescence axis of explants with similar size and growth state, the different placement methods also lead to different culture effects. The explants placed on an inclined plane germinated early, had a large number, and grew extremely fast, while the inflorescence axis placed on a flat surface had almost no bud point differentiation, and the explants placed more than 35 days tended to brown or dry up gradually.

[0022] Table 1

[0023]

[0024] 2. Examples of medium screening

[0025] During the establishment of the in vitro regeneration system of the callus pathway, the success of callus differentiation is the key step of the regeneration system. The rate of callus differentiation directly affects whether the regenerative tissue culture system is feasible. The combination of basic medium and hormone types and concentrations in the callus d...

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Abstract

The invention discloses a method for establishing a rachis in-vitro regeneration system of Heuchera micrantha variety tapestry. The method comprises following steps: (1), an explant is selected and disinfected; (2), after the treated rachis is cut, callus induction culture is performed; (3), callus is subjected to differentiation to induce adventitious buds; (4), the adventitious buds are subjected to subculture multiplication to culture seedlings; (5), test-tube plantlets obtained after subculture multiplication culture are subjected to rooting culture; (6), seedling hardening culture is performed. The Heuchera micrantha variety tapestry is subjected to tissue culture with the propagation method, the adventitious bud proliferation rate in one subculture period (35d) is 8 times or higher,the propagation speed is high, and the problem of seedling breeding is effectively solved.

Description

technical field [0001] The present invention specifically relates to a kind of alum root ( Heuchera micrantha ) Propagation method in tissue culture. Background technique [0002] Alum root ( Heuchera micrantha ), also known as Coral Bell, is a perennial herbaceous flower of the saxifrage family. It has a wide range of horticultural varieties and colorful leaves, and the color of the leaves will change in different seasons, environments and temperatures. It is an ideal perennial flower border material. At present, alum root plants are deeply loved by home gardening small flower lovers, and the e-commerce sales of some varieties are in short supply. In addition, in gardens, it is also used for understory flower borders, ground covers, garden greening, etc., and is an excellent perennial color-leaf flower in a shade environment. [0003] Alum root plants like partial shade, are tolerant to full light, low temperature, and avoid excessive humidity. They are suitable for pla...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 束晓春李乃伟吴尤宏
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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