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A method for the establishment of an alum root inflorescence axis in vitro regeneration system

A technology for in vitro regeneration and inflorescence rachis, applied in plant regeneration, horticultural methods, botanical equipment and methods, etc., can solve problems such as high pollution rate, easy browning, and difficulty in establishing sterile lines

Active Publication Date: 2021-05-18
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The tissue culture rapid propagation system is limited by the explants of the female parent. The contamination rate of the explants is too high after simple disinfection, and browning is easy to occur after excessive disinfection. It is difficult to establish a sterile line

Method used

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  • A method for the establishment of an alum root inflorescence axis in vitro regeneration system

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Experimental program
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Effect test

Embodiment 1

[0030] Explant Screening Example:

[0031] 1. Screening of the placement mode of explants in the culture medium

[0032] For the inflorescence axis of explants with similar size and growth state, the different placement methods also lead to different culture effects. The explants placed on an inclined plane germinated early, had a large number, and grew extremely fast, while the inflorescence axis placed on a flat surface had almost no bud point differentiation, and the explants placed more than 35 days tended to brown or dry up gradually.

[0033] Table 1

[0034]

[0035] 2. Examples of medium screening

[0036] During the establishment of the in vitro regeneration system of the callus pathway, the success of callus differentiation is the key step of the regeneration system. The rate of callus differentiation directly affects whether the regenerative tissue culture system is feasible. The combination of basic medium and hormone types and concentrations in the callus d...

Embodiment 2

[0040] 1. Selection and disinfection of explants: select the inflorescence rachis that has grown for about 20 days as the explants, wash them clean, and then disinfect them. The disinfection process is: rinse them with clean water, rinse them once with Amway detergent, and shake them on a shaker for 15 minutes. -20min; after rinsing with running water for 20-30min, disinfect with 75% ethanol for 10-20s on the aseptic operating table, immediately pour 0.1% mercuric chloride for disinfection for 12min, and finally wash 4 times with sterile water, shaking 3- After 5 minutes, the sterilized inflorescence rachis was cut into 2 cm long segments, and 50 inflorescence rachis segments were inserted in total.

[0041] 2. Induction of callus: take the explant fragments obtained in step (1), and inoculate them in the induction medium (invert the slant of the medium in advance) on a slant to induce callus. The culture temperature is 25±2°C, first cultured in the dark for 10 days, and then ...

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Abstract

The invention provides a method for establishing an alum root inflorescence axis in vitro regeneration system, which is characterized in that it comprises the following steps: selection and disinfection of explants, induction and differentiation of callus, proliferation of adventitious buds, induction of roots, Seedling hardening, tissue culture seedling transplanting, the application method can obtain a large number of aseptic seedlings of alum root, solve the problem of rapid propagation of alum root, within one culture cycle, multiply more than 300 times, the survival rate also obtains 98.71%, the proliferation rate and The improvement of the transplanting survival rate has greatly filled the gap in the market and has broad application prospects.

Description

technical field [0001] The present invention relates to alum root ( Heuchera micrantha ) tissue culture propagation method, which belongs to the technical field of seedling propagation of herbaceous ornamental plants, and specifically relates to a method for establishing an alum root inflorescence axis in vitro regeneration system. Background technique [0002] Heuchera micrantha (Heuchera micrantha), also known as coral bell, is a perennial herbaceous flower of the saxifrage family Heuchera micrantha. Shallow roots. The leaves are base-shaped, broad-hearted, 20-25cm long, dark purple, evergreen in warm areas, small, bell-shaped flowers, 0.6-1.2cm in diameter, red, symmetrical on both sides, prefer partial shade, and tolerate full light; it is often used in gardens It can be used in forest flower borders, ground cover, garden greening and so on. The flowering period is from April to October. It is an ideal perennial flower border material. It is also a perennial flower ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 束晓春王忠严冬琴李乃伟郑玉红庄维兵张凤姣吴尤宏
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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